J Integr Plant Biol. ›› 1986, Vol. 28 ›› Issue (1): -.

• Research Articles •    

A Study on the Application of C17 Medium for Anther Culture

Wang Pei and Chen Yu-rong   

Abstract: Media with varied levels of minor elements and KNO3, NH4NO3, KH2PO4, MgSO4·7H2O, CaCl2· 2H2O and Fe-Salts were screened to obtain high yield of callus from wheat anther by using orthogonal tests. Of all the seven minor elements treated, the one without NaMoO4·2H2O and with 11.2 mg/l MnS04·4H2O was found to be the most effective. Among the five kinds of organic materials tested, biotin appear- ed to have the highest influence on the induction frequency of anther callus and its opimum dosage was 1.5 mg/l. Based on these data, C17 medium was developed and its induction frequency of callus with good quality from wheat anther reached 12.19% in the Institute in 1980. The maximum diffrentiation freguency of anther callus obtained was 50% on C17 medium. C17, medium compositions are as follows (Mg/l): 1400 KNO3, 150 CaCl2-2H2O, 150 MgSO4·7H2O, 300 NH4NO3, 400 KH2PO4, 27.85 FeSO4·7H2O, 37,25 Na2-EDTA, 11.2 MnS04·4H2O, 8.6 ZnSO4·7H2O, 6.2 H3BO3, 0.83 KI, 0.025 CuSO4·5H2O, 0.025 CaCl2·: 6H2O, 8 glycine, 0.5 nicotinic acid, 0.5 thiamine hydrochloride and 1.5 biotin. The re- differentiation medium is supplemented with 2 2,4-D+0.5 KT+7000 agar+90000 sucrose, pH5.8. The dedifferentiation supplemented with 0.5 IBA+2 KT+7000 agar+ 30000 sucrose, pH 5.8.

Key words: Medium, Anther culture, Callus, Induction frequency

Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn
Copyright © 2022 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q
备案号:京ICP备16067583号-22