Abstract: A lipid-depleted cytochrome b 6f (Cyt b 6f) preparation was obtained from spinach (Spinacia oleracea L.) chloroplasts. Upon reconstitution of this preparation with the membrane lipids purified from spinach thylakoid, the effects of different membrane lipids on the electron transfer activity were studied. The results show that the electron transfer activity of Cyt b 6f is obviously stimulated to different extents, respectively,bymonogalactosyldiacylglycerol(MGDG),digalactosyldiacylglycerol(DGDG), phosphatidylcholine (PC), phosphatidylglycerol (PG) and sulfoquinovosyldiacylglycerol (SQDG), and that the extents of stimulation may be closely related to the charge of the membrane lipids. The stimulation of non-charged lipids (MGDG, DGDG) and neutrally-charged lipid (PC) was high with a maximum enhancement of 89%, 75% and 77%, respectively; but the stimulation of two kinds of negatively-charged lipid (PG and SQDG) was relatively low with a maximum enhancement of 43% and 26%, respectively.

Key words: cytochrome b 6f complex, thylakoid membrane lipid, reconstitution, electron transfer