Obtaining a Transgenic Upland Cotton Harboring Two Insecticidal Genes

Abstract

Abstract: pBinLK carried two insecticidal genes, pea lectin (P-Lec)gene and soybean Kunitz trypsin inhibitor (SKTI) gene, were successfully transferred into 4 upland cotton ( Gossypium hirsutum L. ) cultivars, "Xinluzao-1 ", "Xinluzhong-2", "Jihe-321" and "Liao-9" via Agrobacterium-mediated transformation. Hypocotyl segments from aseptic seedlings were used as receipient. After co-cultivation of hypocotyl segments with A. tumefac/ens (Smith et Townsend) Conn, kanamycin-resistant calli were screened, and somatic embryos and regenerated plants were obtained through various media. Transgenic cotton plants harboring two insecticidal genes were confirmed by NPT-Ⅱ ELISA, PCR and PCR Southern. rllae results of bioassay demonstrated that the transgenic plants showed significant resistance to the larvae of cotton bollwonn (Heliothis armigera Hubner).

Key words: Upland cotton, Transformation, Agrobacterium tumefaciens, Insecticidal genes

WANG Wei, ZHU Zhen, GAO Yue-Feng, SHI Chun-Lin, CHEN Wan-Xin, GUO Zhong-Chen and LI Xiang-Hui. Obtaining a Transgenic Upland Cotton Harboring Two Insecticidal Genes[J]. J Integr Plant Biol., 1999, 41(4): -.

[1]	Wenbo Xu, Miao Zhang, Yangcun Li, Wanwan He, Shengchun Li and Jiang Zhang. Complete protection from Henosepilachna vigintioctopunctata by expressing long double-stranded RNAs in potato plastids [J]. J Integr Plant Biol., 2023, 65(4): 1003-1011.
[2]	Caixiang Wang, Juanjuan Liu, Xiaoyu Xie, Ji Wang, Qi Ma, Pengyun Chen, Delong Yang, Xiongfeng Ma, Fushun Hao and Junji Su. GhAP1-D3 positively regulates flowering time and early maturity with no yield and fiber quality penalties in upland cotton [J]. J Integr Plant Biol., 2023, 65(4): 985-1002.
[3]	Boqing Zhao, Yingli Zhou, Xiangmei Jiao, Xi Wang, Baoshan Wang and Fang Yuan. Bracelet salt glands of the recretohalophyte Limonium bicolor: Distribution, morphology, and induction [J]. J Integr Plant Biol., 2023, 65(4): 950-966.
[4]	Zhelong Lin, Lei Chen, Shanjie Tang, Mengjie Zhao, Tong Li, Jia You, Changqing You, Boshu Li, Qinghua Zhao, Dongmei Zhang, Jianli Wang, Zhongbao Shen, Xianwei Song, Shuaibin Zhang and Xiaofeng Cao. Efficient CRISPR/Cas9-mediated genome editing in sheepgrass (Leymus chinensis) [J]. J Integr Plant Biol., 2023, 65(11): 2416-2420.
[5]	Hee-Jeong Jeong, and Ki-Hong Jung. Rice tissue-specific promoters and condition-dependent promoters for effective translational application [J]. J Integr Plant Biol., 2015, 57(11): 913-924.
[6]	Wang Li, Bing Wang, Man Wang, Min Chen, Jing-Ming Yin, Ghullam Murtaza Kaleri, Rui-Jie Zhang, Tie-Niu Zuo, Xiong You and Qing Yang. Cloning and characterization of a potato StAN11 gene involved in anthocyanin biosynthesis regulation [J]. J Integr Plant Biol., 2014, 56(4): 364-372.
[7]	Caiping Cai, Wenxue Ye, Tianzhen Zhang and Wangzhen Guo. Association analysis of fiber quality traits and exploration of elite alleles in Upland cotton cultivars/accessions (Gossypium hirsutum L.) [J]. J Integr Plant Biol., 2014, 56(1): 51-62.
[8]	Jianhui Ma, Hengling Wei, Ji Liu, Meizhen Song, Chaoyou Pang, Long Wang, Wenxiang Zhang, Shuli Fan, and Shuxun Yu. Selection and Characterization of a Novel Photoperiod-Sensitive Male Sterile Line in Upland Cotton [J]. J Integr Plant Biol., 2013, 55(7): 608-618.
[9]	Wenxiang Zhang, Shuli Fan, Chaoyou Pang, Hengling Wei, Jianhui Ma, Meizhen Song, and Shuxun Yu. Molecular Cloning and Function Analysis of Two SQUAMOSA-Like MADS-Box Genes From Gossypium hirsutum L. [J]. J Integr Plant Biol., 2013, 55(7): 597-607.
[10]	Martin A. J. Parry, and Malcolm J. Hawkesford. An Integrated Approach to Crop Genetic Improvement [J]. J Integr Plant Biol., 2012, 54(4): 250-259.
[11]	Jia Liu, Qijie Zheng, Qiuxiang Ma, Kranthi Kumar Gadidasu, and Peng Zhang. Cassava Genetic Transformation and its Application in Breeding [J]. J Integr Plant Biol., 2011, 53(7): 552-569.
[12]	Reza Maali Amiri, Natalia O. Yur'eva, Khristina R. Shimshilashvili, Irina V. Goldenkova-Pavlova, Vasiliy P. Pchelkin, Elmira I. Kuznitsova, , Vladimir D. Tsydendambaev, Tamara I. Trunova, Dmitry A. Los, Gholamreza Salehi Jouzani, and Alexander M. Nosov, . Expression of Acyl-lipid Δ12-desaturase Gene in Prokaryotic and Eukaryotic Cells and Its Effect on Cold Stress Tolerance of Potato [J]. J Integr Plant Biol., 2010, 52(3): 289-297.
[13]	Hai-Hong Shang, Chuan-Liang Liu, Chao-Jun Zhang, Feng-Lian Li, Wei-Dong Hong and Fu-Guang Li. Histological and Ultrastructural Observation Reveals Significant Cellular Differences between Agrobacterium Transformed Embryogenic and Non-embryogenic Calli of Cotton [J]. J Integr Plant Biol., 2009, 51(5): 456-465.
[14]	Lin Cheng, Yijing Zou, Shuli Ding, Jiajing Zhang, Xiaolin Yu, Jiashu Cao and Gang Lu. Polyamine Accumulation in Transgenic Tomato Enhances the Tolerance to High Temperature Stress [J]. J Integr Plant Biol., 2009, 51(5): 489-499.
[15]	Zhong Zhao, Yan Zhu, Mathieu Erhardt, Ying Ruan, and Wen-Hui Shen. A Non-canonical Transferred DNA Insertion at the BRI1 Locus in Arabidopsis thaliana [J]. J Integr Plant Biol., 2009, 51(4): 367-373.

Obtaining a Transgenic Upland Cotton Harboring Two Insecticidal Genes

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments