J Integr Plant Biol. ›› 1998, Vol. 40 ›› Issue (7): -.
• Research Articles •
YONG Wei-Dong, LIANG Tie-Bing, XIE An-Yong, LI Cong and SONG Yan-Ru
Abstract: Using NADPH-dependent acetoacetyl-CoA reductase gene (phbB) and poly-β-hydroxybutyrate (PHB) synthase gene (phbC) cloned from Alcaligenes eutrophus H16 and expression vector pKK223-3, the authors constructed an E. coli expression vector pKCB containing independent phbB and phbC operators, respectively, and transfered it into E. coli JM109. The microscopy and GC analysis indicated that E. coli JM109 (containing pKCB) induced by IPTG could synthesize poly-β- hydroxybutyrate (PHB). By DNA processing, three tuber-specific plant expression vectors, pP- SAGB (containing phbB), pBIBGC ( containing phbC) and pPSAGCB ( containing both phbB and phbC), were successfully constructed. In 5 transformed potato cuhivars, the authors screened 20 positive lines.
Key words: Poly-β-hydroxybutyrate, Expression vector, Transformation potato
YONG Wei-Dong, LIANG Tie-Bing, XIE An-Yong, LI Cong and SONG Yan-Ru. phbB, phbC Gene Expression in E. coli and Their Transformation and Identification in Potato[J]. J Integr Plant Biol., 1998, 40(7): -.
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