Abstract: d sperm cell-rich) were isolated from disrupted pollen of Orychophragmus violaceus (L.) O.F. Schulz with 10 lan nylon filter and sucrose gradient centrifugation. Proteins were extracted using a phenol procedure and polypeptide patterns were analysed with two-dimensional polyacrylamide gel electrophoresis. The sperm cell-ri& fraction had 249 spots and the sample fraction contained 204 spots. The interleaf of sample and 30% sucrose fraction revealed 190 spots, the 30% sucrose fraction 244 spots and the interleaf of 30% and 40% sucrose fraction 167 spots. Compared the spots of the sperm cell-ri& fraction with those of the other four fractions, more than 18 spots were unique to the sperm cell-rich fraction and most of the unique spots were above 60 kD.

Key words: Sperm cell of Orychophragrnus violaceus, Two-dimensional electrophoresis, Special protein