Construction of Molecular Linkage Map in Masson Pine Using RAPD Markers and Megaga  metophytes from a Single Tree

Abstract

Abstract: A set of 300 random ohgonucleotide primers was screened for random amplified polymorphic DNA (RAPD) fragments within a sample of 79 megagametophyte DNAs of a single masson pine ( Pinus massoniana Lamb. ) to generate RAPD markers and construct the molecular linkage map for masson pine. 64 repeatable RAPD markers segregated in a 1 present to 1 absent ratio. Through multipoint linkage analysis, 47 markers were assigned to 13 different linkage groups(pairs). It covered a tdtal genetic distance of over 692.5 cM (centimorgan). The average distance between markers was 14.7 cM. It supplied a linkage framework for a more saturated linkage map of masson pine.

Key words: Random amplified polymorphic DNA, Molecular marker linkage map, Masson pine Megagametophytes

Yin Tong-ming, Huang Min-ren, Wang Ming-xiu, Zhu Li-huang and Zhai Wen-xue. Construction of Molecular Linkage Map in Masson Pine Using RAPD Markers and Megaga metophytes from a Single Tree[J]. J Integr Plant Biol., 1997, 39(7): -.

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Construction of Molecular Linkage Map in Masson Pine Using RAPD Markers and Megaga metophytes from a Single Tree

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