J Integr Plant Biol. ›› 1997, Vol. 39 ›› Issue (2): -.

• Research Articles •    

Genotypic Mapping Using RFLP Markers on Doubled Haploids Derived from a Cross Between indica and japonica Rice

Li Ping, Zhu Li-huang, Zhou Kai-da and Chen-Ying   

Abstract: A rice RFLP map with 233 loci based on a population of 81 doubled haploids (DH) from the indica/japonica hybrid of "Gui 630'/"02428" was used to analyse the genome ratios and graphical genotypes of DH lines. The “Gui 630” genome ratio of the individual DH lines varied from 29.3% to 78.6% with an average of 49% while the ratios of most DH lines ranged between 44% and 49%. Of the mapped RFLP markers 130 showed skewed segregations with a significant deviation from the expected monogenic ratio, but the numbers of the markers deviated towards male and female parent were approximately equal. It was found that the markers with the segregation deviation in the same direction tend to cluster on some chromosomes and some of their regions. The average "Gui 630' genome ratios of different chromosomes in the DH population varied greatly between 29% and 65 %. In addition, several chromosomes were inherited completely from either one of the parents in some DH lines, indicating the rare occurrence of crossover along the pairing homologous chromosomes during meiosis.

Key words: Rice, Hybrid of indica and japonica, Doubled haploid, Graphical genotype, Genome ratio, Distorted segregation

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