J Integr Plant Biol. ›› 1990, Vol. 32 ›› Issue (11): -.

• Research Articles •    

Fractional Selection of Somatic Embryos in the Production of Carrot Artificial Seeds

Zhu Cheng, Huang Mei-juan, Niu Xiao-mu, Li Xiu-qing and Fu Xiao-di   

Abstract: Carrot somatic embryos were selected fractionally from the cell suspension with an equipment of embryonic cell clumps selector. The operation and optimal conditions of this equipment used for selection of somatic embryos (SEs) have been tested and determined repeatedly. Three classes of SEs in different sizes, numbers and morphology were obtained. The rate of fractional selection with this equipment was 872 mature embryos/100 ml cell suspensionlh. These 3 classes of selected SEs were encapsulated in alginate beads to produce artificial seeds. In vitro germination frequencies of these seeds with production of seedling-quality plantlets were tested on MS medium in sterilized condition. In addition seedlings from artificial seeds grew normally to adults in soil pots. These results indicated that the use of selected SEs to produce artificial seeds increases the seed quality obviously.

Key words: Artificial seed, Somatic embryo, Carrot

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