Abstract: In order to culture the regenerated stamens of hyacinth in vitro to maturity, the effects of temperature on regeneration of stamens, microsporogenesis and pollen development were studied. Results showed: the proper temperature for stamen regeneration was 25℃. The temperature going down gradually was advantageous to the microsporogenesis and pollen development. The most suitable temperatures for differentiation of microsporocyte, meiotic division and pollen first mitosis were 20–25℃, 20℃ and 10℃ respectively. Under such temperature condition, it was possible to culture the regenerated stamens to maturity, and pollen grains in the stamens had the higher germination frequency. On the contrary, unsuitable temperature condition will make microsporogenesis and pollen development stop at certain development stage, it will finally result in pollen abortion.

Key words: Hyacinthus orientalis L., Temperature effect, Stamen regeneration, Microsporogenesis, Pollen development