Abstract: The generative cells isolated from pollen grains of Hemerocallis minor Mill. were cultured in modified Ks and MS agarose media surrounded by liquid media containing anthers as a feeder. Microscopical observations on a total of 5961 cells revealed that nuclear divisions occured leading to the formation of 2–4 nuclei within one cell. The average induction frequency among 14 culture experiments was 3.27% and the 11.46% as the highest. The generative cells varied considerably from spindle, oval to spherical shape. Among them the spherical cells appeared easier to be triggered. There were equal and unequal first nuclear divisions with almost equal chance of their occurence. A few of the cellspassed through the second nuclear division, producing 3--4 nucleate cells. Cytokinesis resulting in 2-celled structures seemed to be induced in rare cases. This is the first experimental attempt to culture isolated generative cells and the results show the potential of free generative cells surviving and developing under In Vitro conditions.

Key words: Generative cell, In Vitro culture, Nuclear division, Hemerocallis minor mill.