J Integr Plant Biol. ›› 1990, Vol. 32 ›› Issue (5): -.

• Research Articles •    

Dynamic Change of Amyloplast DNA in Developing Cotyledon Ceils of Lotus

Sun De-lan   

Abstract: It was first shown that the amyloplasts in young cotyledon cells of lotus (Nelumbonucifera Gaertn.) and the isolated amyloplasts stained with Feulgen reaction and treated by DNase, had Feulgen-positive reaction and Feulgen-negative reaction respectively. The isolated amyloplasts stained with DAPI demonstrated blue fluorensence. Some experiments proved that DNA content in the amylopasts increased gradually with the development of cotyledon, showing the dynamic change of DNA in the amyloplasts. Electron microscopic observation revealed that there was no membrane surrounding the DNA region of the amyloplasts illustrating the characteristic of its protokaryotic organisms. The DNA fibrils were about 25A in diameter.

Key words: Young cotyledon ceils of lotus, Feulgen reaction, Amyloplast DNA

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