J Integr Plant Biol. ›› 1989, Vol. 31 ›› Issue (11): -.

• Research Articles •    

Establishment of an Autotetraploid Rice Anther Clone with High Frequency and Long Term Plant Regeneration

Qin Rui-zhen Tong Qing-juan, Xu Zheng and Guo Xiu-ping   

Abstract: Results of study on clone A87203 4C-1-5 from anther culture of homologous tetraploid rice were reported. The trials showed that the clone had a bud multiplication rate of 150–200 times, Under stereoscope, buds were differentiating on buds, forming bud masses. It had a callus induction frequency about 5 times when the bud masses were clipped into pieces and cultured on MS medium for dedifferentiation. Buds with the characteristics of high multiplication were able to be produced from about 50% of the calli when they were transferred onto MS medium for differentiation. It still maintained a vigorous multiplying ability though it had been subcultured for more than 30 generation during the past 1 year or more. Numerous plantlets were able to be produced by culture under regulated temperature and light conditions. A number of plantlets deriving from the buds had been planted in the fields this year. Most of them were normal, however, few plants had morphological variations, with 6 trisomic (2n+1 =25) plants observed during meiotic division of their pollen mother cells.

Key words: Autotetraploidy rice, Anther clone Trisome

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