J Integr Plant Biol. ›› 1989, Vol. 31 ›› Issue (10): -.

• Research Articles •    

Callus Regeneration from Protoplasts of Panax ginseng

Li Le-gong   

Abstract: Protoplasts were enzymatically isolated from the cultured cell line No. 246 of Panax ginseng and suspended in a 67V-D liquid medium. After 35 days of culture, the cell clusters derived from protoplasts were transfered to a 67V-E semisolid medium. When the cell clusters grew to 2 mm approximately, they were transfered to common 67V medium for the callus regeneration.

Key words: Panax ginseng, Protoplast culture, callus regeneration

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