J Integr Plant Biol. ›› 1989, Vol. 31 ›› Issue (9): -.
• Research Articles •
Dong Nin, Wang Li-rong, Lan Lan and Zhang De-yi
Abstract: Barley flour was defatted with petroleum ether and eliminated the salt and water soluble proteins with 2 times 5% NaCl and I time redistilled water extracting. After the above procedures, the hordein I was extracted with 55% isopropanol. The dry hordein I was solublized in 2% SDS and then separated by sephadex G-100 chromatography. The peak 2 hordein's molecular weight was 14–20 kD in SDS-PAGE protein pattern. Authors defined this group of hordein as hordein A. Its lysine content was 2.6%. The further experiments showed that this group of hordein A was soluble easily in isopropanol and alcohol but difficultly in 5% or 0.5 mol/l NaCl solution. It could be suggested that this group of hordein A is prolamine but not salt-soluble protein.
Key words: Hordein A, High-lysine, Prolamine
Dong Nin, Wang Li-rong, Lan Lan and Zhang De-yi. Solubility of the High-Lysine Component of Hordein A[J]. J Integr Plant Biol., 1989, 31(9): -.
0 / / Recommend
Add to citation manager EndNote|Ris|BibTeX
URL: https://www.jipb.net/EN/
https://www.jipb.net/EN/Y1989/V31/I9/