Physical Mapping of Recombinant Plasmids Containing Broad Bean Chloroplast  Ribosomal  RNA Genes

Abstract

Abstract: Two BamHl fragments containing broad bean chloroplast rRNA genes were cloned using the bacterial plasmid pBR322 as a vector and Escherichia coli HB101 as host bacterial. Physical maps of the two cloned ct DNA BamHI fragments containing rRNA genes were constructed by cleavage with several restriction endonucleases and Southern blot hybridization with E. coli 16S-23S rRNAs. Recombinant plasmids pVFBI6 and pVFB32 contain a 16S rRNA sequence on the 4.70 kb BamHl fragment, a 23S rRNA sequence and 4.5S/5S rRNA sequences on the 5.65 kb BamHl fragment, respectively.

Key words: Recombinant DNA, Gene cloning, rRNA genes, Broad bean chloroplast

Li Jla, Tang Su, Zhan Xiao-ming, Chen Qin-chuang, Weng Xing-hua and Wang Xun-ming. Physical Mapping of Recombinant Plasmids Containing Broad Bean Chloroplast Ribosomal RNA Genes[J]. J Integr Plant Biol., 1989, 31(3): -.

[1]	Zhirui Yang and Feng Qin. The battle of crops against drought: Genetic dissection and improvement [J]. J Integr Plant Biol., 2023, 65(2): 496-525.
[2]	Jingyang Gao, Peijiang Feng, Jingli Zhang, Chaopei Dong, Zhao Wang, Mingxiang Chen, Zhongliang Yu, Bowen Zhao, Xin Hou, Huijuan Wang, Zhaokun Wu, Razia Sultana Jemim, Haidong Yu, Doudou Sun, Pei Jing, Jiafa Chen, Weibin Song, Xuecai Zhang, Zijian Zhou and Jianyu Wu, . Enhancing maize's nitrogen-fixing potential through ZmSBT3, a gene suppressing mucilage secretion [J]. J Integr Plant Biol., 2023, 65(12): 2645-2659.
[3]	XU Hong, ZHANG Xiang-Qi, WANG Xian-Ping, GUO Ai-Guang. Molecular Cloning of Agropyron intermedium Low-Molecular-Weight Glutenin Subunit Genes from a Triticum aestivum-Ag. intermedium Addition Line TAI-13 [J]. J Integr Plant Biol., 2004, 46(5): 595-602.
[4]	QIN Qiao-Ping, ZHANG Shang-Long, CHEN Jun-Wei, XIE Ming JIN Yong-Feng, CHEN Kun-Song, Syed ASGHAR. Isolation and Expression Analysis of Fructokinase Genes from Citrus [J]. J Integr Plant Biol., 2004, 46(12): 1408-1415.
[5]	XIA Ming, WANG Xiao Bing, LI Hai Bo, WU Ping. Identification of the Rice Vacuolar ATPase B Subunit Gene and Its Expression Pattern Analysis Under Phosphorus Deficiency [J]. J Integr Plant Biol., 2002, 44(5): 573-578.
[6]	ZHAO Da-Zhong, CHEN Dan-Ying, CHEN Min, LIU Chun-Xiang, YANG Cheng and ZHAI Zhong-He. The Existence of Intermediate Filament in Arabidopsis thaliana Cell [J]. J Integr Plant Biol., 1999, 41(8): -.
[7]	PENG Kai-Man and ZHANG Qi-Fa. Acosmid Library Constructed to the Elite Rice Cultivar [J]. J Integr Plant Biol., 1999, 41(3): -.
[8]	HU Jian-Guang, ZHAO Xiang-Shan, LIU Jun, YUAN Zi-Qiang and YANG Jin-Shui. Isolation and Characterization of a cDNA Encoding Maize Cyto solic Malate Dehydrogenase [J]. J Integr Plant Biol., 1999, 41(1): -.
[9]	Xie An-yong, Cui Xiao-jiang and Song Yan-ru. Cloning and Sequencing of phbB and phbC in Alcaligenes eutrophus H16 and Construction of Their Plant Expression Vectors [J]. J Integr Plant Biol., 1995, 37(8): -.
[10]	Wang Guang-li,Qian Zhong-xing and Lin Zhong-ping. Cloning and Sequencing of the Gene Encoding 10 kD Zein of Maize [J]. J Integr Plant Biol., 1993, 35(7): -.
[11]	Chu Rui-yin,Zhang Xu,Pan Nai-sui and Chen Zhang-liang. The cDNA Cloning and Nucleotide Sequence of the Gene Encoding Nonstrucure Protein of Rice Dwarf Virus Genome Segment 10 [J]. J Integr Plant Biol., 1993, 35(2): -.

Physical Mapping of Recombinant Plasmids Containing Broad Bean Chloroplast Ribosomal RNA Genes

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 11

Recommended Articles

Metrics

Comments