J Integr Plant Biol. ›› 1987, Vol. 29 ›› Issue (4): -.

• Research Articles •    

Induction of Endosperm Calluses and Regeneration of Endosperm Plantlets of Asparagus officinalis

Liu Shu-qiong, Gui Yao-lin, Gu Shu-rong and Xu Ting-yu   

Abstract: The endosperm callus has been induced from the young endosperm of Asparagus officinalis L. on the MS supplemented with auxin. The induction frequency of callus amounts to 65.9%–83.1%. When the callus was transferred to the medium supplemented with lower concentration of NAA 0.1 ppm or containing BA 1 ppm and NAA 0.5 ppm, the differentiation of shoots, roots and a few embryoids began to occur. A few calluses and embryoids can develop into plantlets. The chromosome number in the cells from the same root tip and shoot apex of endosperm plantlet is very unstable. They can be euploids (n=10, 2n=20, 3n=30, 4n=40). or aneupl0ids (n=6, 7, 17, 25, 53).

Key words: Asparagus officinalis, Endosperm culture, Endosperm plantlets

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