Agrobacterium -mediated Transformation of Rice with Help of Bombardment

Abstract

Abstract: Oryza sativa L. ssp. japonica cv. Zhonghua 8, which is recalcitrant to infection of Agrobacterium tumefaciens (Smith et Townsend) Conn strain EHA105 with ordinary binary vector pCambia 1301, was transformed through Agrobacterium mediated transformation with help of bombardment. The transformation efficiency can be raised greatly. Single copy of gene insertion in the genome of transgenic rice plants was proved by Southern analysis and the expression of GUS gene was observed. GUS gene and hygromycin-resistant gene show 3∶1 segregation in progenies of the transgenic rice plants.

Key words: Agrobacterium , bombardment, transformation, rice

MING Xiao-Tian, YUAN Hua-Yi, WANG Li-Jiang, CHEN Zhang-Liang. Agrobacterium -mediated Transformation of Rice with Help of Bombardment[J]. J Integr Plant Biol., 2001, 43(1): -.

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Agrobacterium -mediated Transformation of Rice with Help of Bombardment

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