J Integr Plant Biol. ›› 1998, Vol. 40 ›› Issue (10): -.

• Research Articles •    

Heat- shock Induced Protein in Pea Leaves Which is Immunoreactive to Gro EL Antibody

CHEN Zhong and SU Wei-Ai   

Abstract: A high molecular weight protein was found in pea ( Pisum sativum L. ) seedling by means of Westem-blotting, and it consisted of a ct (60.4 kD) and α β (65.5 kD) subunits. The protein had low ATPase activity. Its expression could be enhanced by 3 to 4 folds under heat-shock stress, but was not affected by exogenous ABA. The results of localization and 35S-Met labeling showed that it was a cytoplasmic protein and its synthesis was not inhibited by chloramphenicol.

Key words: Gro EL, Western-blot, Heat-shock, Pea

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