J Integr Plant Biol. ›› 1985, Vol. 27 ›› Issue (3): -.

• Research Articles •    

Cellular Networks in Epidermal Peels of Onion

Chen Zi-qing, Zhang Wei-cheng and Lou Cheng-hou   

Abstract: Cellular networks ill epidermal peels of onion bulb can be distinguished by first removal of superficially attached cytoplasmic constituents with Triton phos- phate buffer and then by staining with Coomassie blue R 250(Fig. 4). Two distinct kinds of networks can be further recognized by treatment with colchicine and cytochalasins: one thinner network underneath the periphery of plasmalemma can be abolished by colchicine (Fig. 7, 8); and the other thicker one which associates tangentially with the nucleus was more distinctive after cytochalasin B treatment (Fig. 5, 6). Discussion is made regarding these two networks in wall-enclosed plant cells as revealed by the present technic.

Key words: Network, Microtubule, Fibrils, Cytochalasin B, Colchicine, Onion, Coomassie blue R 250

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