February 1985, Volume 27 Issue 2


          Research Articles
A Comparative Study of Peroxidase Isoenzymes between Resistant and Susceptible Wheat Varieties to Powdery Mildew
Author: Jin Hua-fen and Shen Yang
Journal of Integrative Plant Biology 1985 27(2)
Abstract (Browse 1870)  |  Full Text PDF       
Cytochemical Localization and Histoelectrofocusing of Nonspecific -Esterase in the Scutellum and the Aleurone Layer of Oat Seeds
Author: Xu Shi-xiong (S.Y. Zee) and Chen Qing-rang H. Y. Chan
Journal of Integrative Plant Biology 1985 27(2)
Abstract (Browse 1729)  |  Full Text PDF       
Microfossil Assemblages from Upper Carboniferous in Ordos Basin, Northwest China
Author: Geng Guo-cang
Journal of Integrative Plant Biology 1985 27(2)
    This paper is resulted from a systematic study of sporo-pollen of Ordos Basin of Northwest China has been found. Single spore assemblage in the Taiyuan Formation contains abundant Pteridophic and Pteridosperm spores and a few Gymnosperm pollen. 11 new species are described. New sporopollen taxa include, Punctatisporites gansuensis, Auritulinasporites ningxiaensis, Gulisporites discersus, Trivolites scabratus, Psomospora anulatus, Gravisporites rugu- laris, Velamisporites breviradidlis, Murospora strialatus, Foveomonoletes foveolatus, Cy- clogranisporites microtriagulus (comb. n.), Cycadopites pachyrrhachis (comb. n.) and Punatatisporites sp., Tantillus sp., Converrucosisporites sp., Columnisporites sp.. and Zono- spheridium reticulatoides and Z. granuloides of Acritarchs. There are some differences between spore assemblages of eastern and western parts of Ordos Basin. Western part is characteristic by more abundance of cingulum spores. The age of the microfossil is considered to be of the Late Carboniferous.
Abstract (Browse 1906)  |  Full Text PDF       
Studies on the Genus Ullmannia from South China
Author: Feng Shao-nan and Zhu Jla-nan
Journal of Integrative Plant Biology 1985 27(2)
    This paper deals with the taxonomy of the genus Ullmannia. The specimens of U. aff. bronnii or U. cf. bronnii previously recorded from South China may be regarded as U. bronnii, This paper also states the stratigraphical and geographical distributions of Ullmannia and diseusses the palaeoelimate and the palaeogeography of South China from the late early Permian to the end of Permian. Moreover, this paper states the general eh.araeter of U. bronnii from South China and describes the Cathaysiopteris crassinervis sp. nov.
Abstract (Browse 1857)  |  Full Text PDF       
Studies on the Distribution Characteristic of Mineral Elements in the Stem of Pinus tabulaeformis
Author: Ren Ji-kai, Chen Ling-zhi, Miao You-gui and Kong Fan-zhi
Journal of Integrative Plant Biology 1985 27(2)
    The element analysis of annual rings shows the characteristic of the transfer and distribution of mineral elements in the stem, it may be useful to promote understanding the process of mineral cycling in the forest ecosystem. Analysing the elements in the annum ring and bark of stem disk of 28 aged pine tree, the significant negative correlation exists between the concentration of Ca, K, Mg, Na, Mn, and the age of annual rings. The significant positive correlation of the contents of P, Cu with the age of annum ring appears as well. The positive correlation exists between the contents of Ca, K, Mg, Na, P, Fe, Mn. Al, Zn, Cu, N in bark and the age of the stem bark. The mineral ash of inner bark is relatively high, it is 3.3-fold and 13-fold higher than that in bark and xylem respectively.
Abstract (Browse 1754)  |  Full Text PDF       
The Chemical Constituents of the Roots of Mallotus apelta LOUR Muetl -Arg
Author: Shan Xue-qin, Feng Lian-bin and Wu Cheng-shun
Journal of Integrative Plant Biology 1985 27(2)
    Five crystalline compounds A, B, D, E, F were isolated for the first time from the roots of Mallotus apelta were identified as erythrodiol-3-acetate for A, -sitosterol for B, 3, 29-dihydroxylupane for D, ursolic acid acetate for E, except for F, via chemical and spectroscopic analysis. It was shown that the crystallines of A, B, D, E, and F possessed some bacteriostatic activities on Staphylococus aureus, Bacillus subtilis, Escherichia coli and Bacillus pyocyaneum.
Abstract (Browse 2029)  |  Full Text PDF       
The Fragrance Components of the Jasrninnm sambac (L.) Alton collected by Simultaneous Steam Distillation and Solvent Extraction
Author: Sun Shou-wei, Ma Ya-ping and Wu Cheng-shun
Journal of Integrative Plant Biology 1985 27(2)
    The volatile fragrance components of Jasminum sambac (L.) Aiton flower were collected by a modified simultaneous steam distillation and solvent extraction apparatus (SDE). The obtained extract was concentrated to 100 L by the gas entrainment method in which the extract was distilled under reduced pressure with the influence of a steam of nitrogen. The concentrate was analyzed by GC-MS with PEG-20 M capillary column, and the Kovats indexes were determined at the same time whereby the nalkanes were added into extract. 28 compounds were identified. The major components were: linalool, benzyl acetate, ciscaryophyllene, cis-3-benzyl benzoate, methyl anthranilate and indole. These results show the components of the extract have no qualitative difference from absolute oil. The advantages of the above procedure were discussed in detail.
Abstract (Browse 2011)  |  Full Text PDF       
Studies on the Distribution of Fatty Acids in the Oils of the Family Lauraceae
Author: Wang Jing-ping, Meng Shao-jiang and Li Jing-min
Journal of Integrative Plant Biology 1985 27(2)
    Forty-nine species of seed oils and 6 species of pulp oil from 11 genera were determined and the fatty acid composition of these oils was examined by gas liquid chromatography. The 11 genera belong to four tribes of subfamily Lauroideae. The major fatty acids of the seed oils from the tribe Cinnamomeae (Cinnamomum, Sassafras) and Litseeae ( Actinodaphne, Litsea, Neolitsea, Lindera, Laurus ) are saturated and unsaturated C10, C12, C14 acids except that of Neocinnamomum candatum (Nees) Merr. The seed oils from tribe Perseae (Machilus, Phoebe) and Cryptocaryeae (Cryptocarya) contain more than a trace of C10, C12, C14 acid. The major acids are 16:0 and unsaturated C18 acids. The component acids of pulp oils are different from that of the seed oils of the same species. The oils contain high proportion of palmitic, oleic and linoleic acid. The unsaturated C10, C12, C14 acids from seven seed oils (Litsea cubeba (L.) Pers., L. auriculata Chien et Cheng, Neolitsea chuii Merr, N. cambodiana Lec., Lindera rubronervia Gamble, L. praecox (Sieb. et Zucc.) Bl., L. reflexa Hemsl.) were separated and id- entified. They are cis-4-decenoic, cis-4-dodecenoic and cis-4-tetradecenoic acids. The three acids have not been found in the seed oils of any other plant family.
Abstract (Browse 1928)  |  Full Text PDF       
Purification and Characterization of Low Molecular Weight RNA of Chloroplasts
Author: Cheng Zhen-qi, Zhang Hung Gu Li-ya, Zhao Kun and Cao Wei-guo
Journal of Integrative Plant Biology 1985 27(2)
    Purified intact chloroplasts were obtained from plant leaves by homo- genizing at low speed and filtration. The chloroplast RNA were extracted from the chloroplasts with a mixture of extracting buffer and phenol. The chloroplasts of Oenan the stolonifera DC. contain not only 5S RNA and 4S RNA but also 4.5S RNA among low molecular weight RNA. However there are only 5S RNA and 4S RNA in chloroplasts of Metasequoia glyptostroides and Ginkgo biloba leaves.
Abstract (Browse 1908)  |  Full Text PDF       
The Positive Correlations Between the Activity of Superoxide Dismutase and Dehydration Tolerance in Wheat Seedlings
Author: Wu Ban-gan and Glenn W.Todd
Journal of Integrative Plant Biology 1985 27(2)
    Damage to crops by drought is still a serious problem in large areas of the world. Considerable research has been undertaken to discover the mechanisms of drought injury and drought resistance of plants. However, the critical features of drought injury have not yet been identified. In the past ten years a free radical hypothesis has been suggested to account for subcellular damage caused by severe environments. Superoxide (oxygen radical) is normally produced in hydrated tissues. It is controlled by free radical scavenging reactions. One such scavenger is the enzyme superoxide dismutase (SOD). Under water stress, production of excess free radicals may occur in dehydrated plant tissues and this probably damages the membranes by causing peroxidation of the lipid components. So far few studies have been done to determine if drought injury is correlated with the free radical mechanism. In the present study, the SOD activities in wheat seedlings under water stress have been investigated by measuring the photoreduction of nitro blue tetrazolium using a spectrometric method. Meanwhile, the viabilities of wheat seedlings during drying were followed by tetrazolium test. These results provided information on the relationship between SOD activity and the dehydration tolerance of the plant. Results indicated that SOD activity changed with the time after germination. The activity of SOD of 24 h seedlings was 1.9 times higher than those of 72 h seedlings based on fresh weight. SOD activity in shoot was also higher than in root. These results were consistent with the results obtained from rating of the viabilities of seedlings during drying. The 24h seedlings were more tolerant of dehydration than 72 h seedlings and root were more sensitive of drought than shoot. In addition, shoot and root tips showed the higher SOD activities than non-tip region and they also showed a higher survival ability upon dehydration. In dehydration and subsequent rehydration, SOD activity, different from many other enzymes in plants, increased rather than declined during drying. After rehydration SOD activity returned to nearly the original level. Therefore, the positive correlations were found to exist between SOD activity and dehydration tolerance. It is reasonable to suggest that SOD enzyme may play a protective role against damage caused by free radicals which may be produced excessively during dehydration in wheat seedling.
Abstract (Browse 1965)  |  Full Text PDF       
Chemical Structure of Macrocalyxoformin D
Author: Wang Zhao-quan Wang Xian-rong and Dong Jin-guang and Xu Guang-yi
Journal of Integrative Plant Biology 1985 27(2)
    Macrocalyxoformin D, an new diterpenoid having a spiro secokaurene- skeleton, was isolated from the leaves of Rabdosia macrocalyx (Dunn.) Hara from (Labiatae) collected in the south of Anhui. On the basis of chemical and spectroscopic data, the chemical structure of macrocalyxoformin D was established as (). Macroca- lyxoformin D was shown to have inhibitory action on Hela cells, staphylococcus aureus and bacillus hay in vitro.
Abstract (Browse 1698)  |  Full Text PDF       
Effects of Sulfur and 6-Benzvladeine on the Nitrate Reductase Activity in Rice Seedlings
Author: Zang Ying-ju, Liu Zhen-sheng and Xiao Ping
Journal of Integrative Plant Biology 1985 27(2)
    The effects of sulfur and 6-Benzyladeine on the nitrate reductase activity in rice seedlings were studied by the water culture method. The activity of nitrate reductase was decreased, when plants were grown in sulfur deficient solution. Both sulfur deficient plant and the control were treated in nutrient solution with 6-Benzyladenine concentration of 0.01, 0.1 or 1ppm. It was found that the nitrate reductase activity of former plant was increased, while the activity of the latter one was decreased. When the plants were treated in untrient solution with 6-Benzyladenine concentration of 1 ppm, the transformation of inorganic sulfur to organic compounds was markedly increased in the sulfur deficient plant. However it was decreased in control plant.
Abstract (Browse 1706)  |  Full Text PDF       
Studies on the Callus Formation of Mesophyll Protoplast from Gentiana scabra Bunge
Author: Zhou Yun-luo Qian Ying-qian (Y . C. Chien), Cai Qi-gui, Zhang Zhi-guo and Yan Xiao
Journal of Integrative Plant Biology 1985 27(2)
    Protoplasts were isolated from mesophyll cells of shoot cultures of gentian (Gentiana scabra Bunge). First division occurs after 4C5 days in culturing and small calli are visible to the naked eye within one month. Low temperature pretreatment of source materials is the important factor in connection with the division of mesophyll protoplasts of gentian. It seems that sustain division is within the limits of the protoplasts of small size only. Calli derived from protoplasts are being differentiated.
Abstract (Browse 1817)  |  Full Text PDF       
The Relations Between Chromosome Aberration and the Intercellular Chromatin Migration in Pollen Mother Cells of Vicia faba
Author: Wang Xin-yu and Zheng Guo-chang(Cheng Kuo-chang)
Journal of Integrative Plant Biology 1985 27(2)
    Squash, paraffin section and chromosome banding technique were used to observe the condition of the intercellular chromatin migration (cytomixis) and chromo- some aberration in pollen mother cells (PMCs) of Vicia faba. The following phenomena were found in our experiments; 1. Cytomixis also occurred at synizesis stage. 2. 8.08% of PMCs had a chromosome number deviating from their normal numbers (n=6). 3. The changes in chromosome structure included the chromosomes with a increased or decreased length and chromosome bridges. 4. Appearance of anucleate and binucleate PMCs. Plenty of evidenee shows that the last three phenomena are related to the first one.
Abstract (Browse 1730)  |  Full Text PDF       
Studies on Nucleic Acid and Protein Synthesis and D evelopment of Male Gametnphyte of Clivia nobilis Cultured in Vitro
Author: Tang Pei-hua and Zhu Ying-min
Journal of Integrative Plant Biology 1985 27(2)
    A simple method for culture male gametophyte (MG) of Clivia nobilis in vitro was established and the process of their development was observed. After research on dynamic of nucleic acid and protein synthesis of MG in various developmental stages by using of inhibitors and autoradiography authors found that DNA synthesis fro mrelease of tetrad to sperm only takes place in nucleus of interphase. There is no 3H-tymidine incorporation into vegetative nucleus (Vn), generative nucleus (Gn) or nucleus of sperm in 96 hours before dehiscence of anthers (BDA). The dynamic of protein synthesis is similar to the same of RNAs. Both of them have three peaks and two intermissions. The 1st peak is in 12C9 days BDA. The 2nd is in 7C5 days BDA and intermitted from 48 hours BDA. The 3rd begins from the 1st hour after culture (AC) decleases at 6th hour AC and stops before 20th hour AC. The kind of inhibitor, the time and quanlity of treatment are affected the morphogenesis, showing the relationship to synthesis among DNA, RNA and protein and to the same between biomacromolecular and morphological development of MG.
Abstract (Browse 1760)  |  Full Text PDF       
Observations on the Ultrastructure of the Root Nodules and Its Endophyte of Elaeagnus mollis Diels
Author: Wang Yi-yan, Du Da-zhi, Cui Guo-liang and Li Rong-er
Journal of Integrative Plant Biology 1985 27(2)
    The ultrastructure of the root nodule infecting cell of Elaeagnus mollis Diels and its shapes of the endophyte were observed by light microscope, scanning electron microscope and transmission electron microscope. The endophyte in the root nodules of Elaeagnus mollis D. has five different developing forms: hyphae, nitrogen fixing vesicles, sporangia, sporangio-spores and bacteroid-like cells. The roles played by the different forms of the endophytes in symbiotic nitrogen fixation arc discussed also.
Abstract (Browse 1911)  |  Full Text PDF       
Anatomical Studies of After-Ripening of Coptis chinensis Seed
Author: Li Zheng-li Lee Cheng-lee and Li Rong-ao
Journal of Integrative Plant Biology 1985 27(2)
    The seeds of Coptis chinensis Franeh took 9C10 months to germinate after they were collected and buried under ground.. This is due to the fact that the embryo of Coptis chinensis was not fully developed after shedding but remained in the stage of globular or early heart-shaped embryo, and the latter of which has to pass through a rather long period before it developed into torpedo-embryo stage. Embryo completed its growth about January of the following year. The delay in seed germination is because of the embryo not being fully developed after shedding. However, the primordium of the first foliage leaf initiated early in the apical meristem of the young embryo. Perceiving the whole process of the young embryo growth, it seems possible that, if a suitable condition is envisaged to shorten the heart-shaped embryo stage, the whole germinating process of seed might be somewhat curtailed.
Abstract (Browse 1962)  |  Full Text PDF       
Studies of Karyotypes in Three Wild and Four Cultivated Species of Gossypium
Author: Nie Ru-zhi and Li Mao-xue
Journal of Integrative Plant Biology 1985 27(2)
    Karyotypes in seven species of Gossypium, G. thurberi, G. advidsonii, G. raimondii of D group; G. herbaceum, G. arboreum of A group; G. hirsutum and G. ba- rbadense of AD group were studied in 1983. It can be simplified as follows: G. thurberi 2n = 2x = 26 = 24m + 2Sm (2SAT); G. davidsonii 2n= 2x = 26 20m+6Sm(4SAT); G. raimondii 2n=2x= 26= 20m+6Sm(2SAT); G. herbaceum 2n = 2x = 26 = 18m + 4Sm+4St(4SAT); G. arboreum 2n = 2x = 26 = 18m + 6Sm (2SAT) + 2St(2SAT); G. hirsutum 2n = 4x =52 = 32m + 18Sm(4SAT) +2St (2 SAT); G. barbadense 2n = 4x = 52 = 38m + 12Sm (2SAT) + 2St(2SAT). This paper also deals with the supplier in A group and D group of tetraploids.
Abstract (Browse 1943)  |  Full Text PDF       


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