April 1985, Volume 27 Issue 4


          Research Articles
The Vertical Distribution of Blade Density in Flying Sowing Stand of Pinus yunnanensis Franch
Author: Lin Hong-rong and Jiang-Hong
Journal of Integrative Plant Biology 1985 27(4)
Abstract (Browse 1834)  |  Full Text PDF       
Influence of Cold Pretreatment on the Production of Pollen Callus in Anther Cultures of Barley
Author: Huang Bin
Journal of Integrative Plant Biology 1985 27(4)
Abstract (Browse 1834)  |  Full Text PDF       
Study on Spikelet Budding Directly from Immature Panicle Culture in Rice
Author: Ling Ding-hou, Chen Wan-ying, Chen Mei-fang and Ma Zhen-rong
Journal of Integrative Plant Biology 1985 27(4)
Abstract (Browse 1797)  |  Full Text PDF       
The Significance of the Found Fossil Species of Wodehouseia in China
Author: Zhou Shan-fu
Journal of Integrative Plant Biology 1985 27(4)
    Fossil pollen of Wodehouseia were first found by E. A. Stanley in 1961.There are several species of Wodehouseia found from the drill cores of the Lower member of Taizhou Formation in Rutung, North Jiangsu. It provides an evidence forjudging the Lower member of Taizhou Formation to be Maestrichtian age. In the assemblage of the Lower member of Taizhou Formation, there are not onlyAquiapollenites and Wodehouseia but also Morinoipollenites and Jianghanpollis. TheAquilapollenites and Wodehouseia are typical genera occurred in the humid, subtropicalclimatic phytogeoprovince in the ¡°Aquilapolles District¡±, circumpacific and Siberiaduring Late Cretaceous age. The Morinoipollenites and Jianghanpollis are typical ge-nera occurred in the arid(or semiarid), subtropical climatic phytogeoprovince in widesouthern areas of our country at that time. This mixed assemblage provides a key tocompare the assemblage in wide southern areas of our country with those in other countries. The author discusses the geographical distributions and distributions of geologicalperiod of Wodehouseia in detail. The structure of Wodehouseia and the paleoecology of the parent plant of Wodehouseia pollen are explored. 7 species including 3 new spe-cies are described in this paper. The new species are Wodehouseia jiangsuensis sp. nov., W. circinata sp. nov., W. zoros sp. nov.
Abstract (Browse 1913)  |  Full Text PDF       
Huia Recurvata --- A New Plant from Lower Devonian of Southeastern Yunnan China,
Author: Geng Bao-yin
Journal of Integrative Plant Biology 1985 27(4)
    A new genus and species Hula recurvata is described from the Posong-chong Formation (Siegenian) of Lower Devonian in Wenshan district, Yunnan, China.This plant possesses pseudomonopodial and dichotomous axes with terminal spikes andit sometimes has single sporangia below the branching point within the fertile regions.Sporangia with stalks are ovate, adaxially recurved and downwards and spirally arrangedon the axis of the spike. Protostele is centrarch. The characteristics stated above showthe new plant has close affinity to Taeniocrada decheniana, but it differes from the members of any other rhyniophytes as well as zosterophyllophytes. A new family¡ª¡ªTaeniocradaceae can be established and is provisionally assigned to Rhyniophytina. Evolutionary line of early land-plants, from Rhyniaceae to Taeniocradaceae and then to Bari-nophytaceae is discussed in this paper.
Abstract (Browse 2343)  |  Full Text PDF       
Head Space Constituents of Different Varieties of Osmanthus fragrans
Author: Zhu Mei-li, Ding De-sheng, Huang Zu-xuan, Song Zhi-ying and Lin Yiu-fu
Journal of Integrative Plant Biology 1985 27(4)
    This work deals with the head space constituents of different varietiesof Osmanthus fragrans. A porous crosslinked polystyrene resin (Amberite XAD-4)trap was used to absorbing the head space of fresh flowers and the constituents weredetermined by using GC, GC/MS/DS. Thus, fifty six components have been identifiedand thirty seven of them were unreported: i.e. ethyl acetate, 3-methyl butanone, 3-hydroxy-2-butanone, 5-hexen-2-one, 3,3-dimethyl hexane, undecan-6-one, myrcene, decane,limonene,¦Áand ¦Â-ocimene, 6-methyl-3, 5-heptadien-2-one,1,6-diacetoxy-hexane,5-phen-ylmethoxy-l-pentanol,3-eyelohexene-l-methanol, ,7-dimethyl-3,5-octadiene, menthone,menthol, ethyl benzaldehyde, 2-cyclohexen-l-yl-2-methyl-5-(1-methylethenyl) acetate, 4-methyl-2-heptanol, 1,4-benzene dicarboxaldehyde, hexyl butanoate, ethyl benzoate, cin-namic aldehyde, 1,4-dimethyl-3-eyclohexen-l-ol ethanone, carvone, 2-hydroxymethyl be-nzoic acid, benzoic acid, 3,4-dihydro-l-2(H)-naphthalenone, 8,8-dimethyl-4-methylene, 1-oxaspiro-[2, 5]-oct-5-ene, 2,4-dimethyl phenyl ethenone, 1-ethoxyethyl benzene, 4,6,6-tri-methyl-bicyelo-[3, 1, 1]-hept-3-en-2-one, 1,4-phenylene bis ethanone-l,1, diethyl O-phtha-late and dibutyl-O-phthalate.
Abstract (Browse 2351)  |  Full Text PDF       
The Structure of Remsamabilinin B
Author: Rui He-kai, He Qing-ying, Yu Qiu-mei, Gan Li-xian and Chen Yu-qun
Journal of Integrative Plant Biology 1985 27(4)
    A new cucurbitacin glycoside, named hemsamabilinin B (2), was isolat-ed from Hemsleya amabilis Diels. 2 was hydrolyzed by enzyme on acid to produce 23,24-dihydrocucurbitacin F (3) or D-glucose respectively. On the basis of the spectrosco-pic data of 2 and its acetate (4) (1H NMR, 13CNMR and MS), the structure of 2 wasassigned as 2-0-¦Â-D-glucopyranoside of 3.
Abstract (Browse 1900)  |  Full Text PDF       
The Isolation and Characterization of Triterpenoid Constituents from Maytenus confertiflora Lou et Chen
Author: Wang Xue-fen, Pu Quan-long, Chen Jia-yuan, Wei Rong-fang and Jiang Da-qu
Journal of Integrative Plant Biology 1985 27(4)
    A new triterpenoid confertiflorol along with other two triterpenoids(canophyllol and wieforlide A) have been isolated from the stems of Maytenus conferti-flora Luo et Chen (Celastraceae). The chemical structure of confertifiorol, as 28, 29-dihydroxyfriedelan-3-one, has been confirmed on the basis of chemical studies and spectrometric analysis.
Abstract (Browse 1935)  |  Full Text PDF       
Effect of Zinc on the Contents of Tomato Chloroplast DNA, RNA, Protein and Chl-Protein Complexes in Relation to Light
Author: Zhang Gui-chang, Wu Zhao-ming and Cui Cheng(Tsui Cheng)
Journal of Integrative Plant Biology 1985 27(4)
    Changes of chloroplast DNA, RNA and protein contents in normal andzinc deficient tomato plants grown under different light intensity were studied in thispaper. It was shown that there is no much difference between normal and zinc deficient chloroplast DNA, RNA and protein e0ntents when the plants were grown underlower light intensity, while the DNA, RNA and protein contents of zinc deficient plantsdecreased dramatically under higher light intensity. The DNA and protein contentsof normal plants increased when light intensity was increased from 18000 lx to 45000 lx.The P700 Chl a-protein complexes and light harvesting Chl a/b-protein complexes of zincdeficient plants decreased seriously under higher light intensity.
Abstract (Browse 1959)  |  Full Text PDF       
Changes of Abscisic Acid and Gibberellin Contents during the Release of Dormancy in Winter Buds of Populus tomentosa Carr.
Author: Tan Zhi-yi, Dong Yi-min, Gao Xiu-ying and Fang Yao-ren
Journal of Integrative Plant Biology 1985 27(4)
    Changes of abscisie acid (ABA) and gibberellin (GA3) contents during the release of dormancy in winter bud of Populus tomentosa Carr. were determinedwith GC. After leaf fall in autumn, content of ABA in the bud was 888.0 ¦Ìg/kg. fr.wt. Obvious decrease in ABA content was observed during the bud released from dormancy. The bud kept in room temperature opened about two months earlier thanthat under natural condition; and the rate of decrease of ABA content in these budwas also more rapid. The ABA contents of buds with similar outer appearance werecompared, either the bud from outside under natural condition or under room temperature, they were similar, although time of their occurrence was quite different, withalmost a difference of about two months. From this fact it has been assumed that thereis a close relationship between the release of dormancy and the decrease in ABA content. Another fact was noticed before Dec. 6, no GA3 could be detected in the dormantbud. From Jan. 9 and thereafter, GA3 content increased gradually and reached itsmaximum (20 ¦Ìg/kg. fr.wt) by Mar. 19, the bud was inflated. GA3 decreased again as the bud was opened, it seems to be that the process of releasing dormancy in bud mightbe promoted by GA3.
Abstract (Browse 1935)  |  Full Text PDF       
Proteins of Developing Rice Embryos As Characterized by Twodimensional Gel Electrophoresis
Author: Miao Guo-hua and Tang Xi-hua Tang Si-hua
Journal of Integrative Plant Biology 1985 27(4)
    Developmental changes of rice (Oryza sativa, subsp, japonica) in embryonic proteins during embryogenesis were investigated by modified two-dimensionalpolyacrylamide gel electrophoresis. The results indicated that there were apparently differences in the embryonic proteins between the embryos at 7th and 13th day after anthesis. Some proteins only appeared in the embryos al 7th day, disappearing at 13th day. Then some new proteinsappeared at 21th day embryos, which were different from that disappeared during differentiation. In concomitant with the completion of embryo differentiation, the number of acidic proteins decreased, while the basic ones showed an increasing trend. Itwas also found that in the 7th day embryos, there were a higher relative percent ofembryonic protein spots in the region of higher molecular weight, while in the 13thday there were higher relative percent of ones in the region of lower molecular weight.The electrophoretic pattern of rice germ lectin (RGL) showed that the synthesis ofRGL was associated with embryo differentiation. According to these results, we propose that some of embryonic proteins which areonly found at early stage of embryogenesis may be important factors for the regulationof embryo differentiation. Although the function of these proteins is still an openquestion, these specific proteins, at least, represent an excellent mark for plant embryodifferentiation.
Abstract (Browse 1952)  |  Full Text PDF       
Selection of Streptomycin-Resistant With Abnomal Flower Mutant and Their Progeny of Nicotiana tabacum
Author: Chen Jia-yu, Xu Jin-xiang, Wang Lian-qing, Zhao Shi-min and Wang Pei-tian
Journal of Integrative Plant Biology 1985 27(4)
    Calluses from the germinated seeds of N. tabacum were treated withstreptomycin and erthromycin. 60 regenerated plantlets from the calluses treated withstrepomycin were obtained. According to the flower shape these plantlets were classified into three kinds as follows: 1. Normal flower type, 2. Abnormal flower type, 3.Both normal and abnormal flower in same plant. It was found that the abnormal flowercould not self fertile but could be pollinated by other flower. The progenies of thenormal X abnormal flower were observed. Some of them Showed abnormal flowers. Ithad been suggested, therefore that stretomycin may not only induce mutant of chloroplast but also induce mutant of nucleus because the characters of flower axe controlledby nucleus.
Abstract (Browse 2142)  |  Full Text PDF       
Preliminary Studies of Chromosome Morphology of Several Wild and Cultivated Eggplants
Author: Wu Shi-bin and Li Zheng-li (Lee Cheng-lee)
Journal of Integrative Plant Biology 1985 27(4)
    This paper deals with the chromosome morphology of three wild eggplants (Solanum indicum L., S. indicum L. var. recurvatum C. Y. Wu et S. C. Huang,S. coagulans Forsk.) grown in Shi-shuang-banna and two cultivars (S. melongena L.var. serpentinum Bailey, S. melogena L. var. esculantum Nees). All their chromosomenumbers are 2n=24. The chromosome idiograms of wild eggplants axe as follows: Solanum indicum,2G+12J+2I+8M; S. indicum var. recurvatum, 8G+12J+2I+2M; S. coagulans ( No. 20),10J+2I+12M; S. coagulans(No. 21), 8J+14M+2L; and those of cultivars are: S. melongena var. serpentinum, 6G+12J+2I+4M; S. melongena var. esculantum, 12J+2I+10M. The chromosome idiograms among three wild eggplants are quite different. However, they are rather closely related in each other between the two cultivars. Butthe relative length and the relative position of the sat-chromosomes in each chromo)meidiogram are conspicuously variable.
Abstract (Browse 2124)  |  Full Text PDF       
Comparative Wood Anatomy of Three Species of Normal and Dwarf Pines
Author: Li Zheng-li (Lee Cheng-lee) and Zhang Xin-ying
Journal of Integrative Plant Biology 1985 27(4)
    Wood structure of three species of normal and dwarf pines, i.e. Pinusthunbergii, P. tabulaeformis and P. densiflora, were comparatively studied. The degreesof breadth of annual rings vary in all three species, whether normal or dwarf. In general, after the initiation of growth annual rings gradually increased in breadth andreached maximum by the 3td or 4th year, and then decline to a certain degree of breadthin subsequence. However, the annual rings of normal pines are wider than those ofdwarf pines. There are many false annual rings mingled with annual rings in dwarfpines. The length and breadth of tracheids exhibited remarkable variation among annualrings. Normal pine tracheida are longer and wider than those of dwarf pine and thedifferences of breadth are more significant. Nevertheless there is no correlation between the width of annual rings and the length or breadth of tracheids. These findingsmight indicate that a favorable growth condition not only can promote the growth of:pines but also can avert tracheids shortening.
Abstract (Browse 2110)  |  Full Text PDF       
Ultrastructural Studies on the Degeneration Processes in Wheat Nucellar Cells
Author: You Rui-lin
Journal of Integrative Plant Biology 1985 27(4)
    During its development and enlargement, the embryo sac encroachesupon adjoining nucellar cells continuously, leading to their degeneration completely. Thefirst signs of degeneration appear in the vacuolated nucellar cells at a distance to theembryo sac. In the process, two types of degeneration are found in wheat nucellarcells. In type I the degeneration is carried out through the in situ autolysis of theircytoplasm. The ribosome density decreases drastically in the cytoplasm of the nucellarcells. Organelles therein become suspended in a more diluted cytoplasmic matrix. Debris of dissolved cytoplasmic components distribute throughout the cytoplasm andvacuoles. Chromatin masses in the nucleus decrease obviously or chromatin becomecondensed in the mean time. Degenerate cells of this type become empty and dead atlast, lying on the border of the embryo sac. Type ¢ò of degeneration is quite contrary to the first, the process is carried out through the proliferation and dilation ofrough endoplasmic reticulum cisternae, and their engulfment and sequestering of thecytoplasmic components. Degeneration type ¢ò is associated with the increasing affinityof dyes, such as lead, in the protoplast. Finally, plasma membrane and tonoplast breakdown, protoplast shrinks and converts to a dark disorganized mass. During the process of the degeneration type ¢ò, a series of complicated changes occur in the nucleusand cytoplasm.
Abstract (Browse 1944)  |  Full Text PDF       
Study on the Chemical Constituents of Lancan tibetica Hook F. et Thoms
Author: Zheng Run-de, Wang Mao-tian and Zhang Hui-di
Journal of Integrative Plant Biology 1985 27(4)
    A new lignancide named lantibeside (1) was isolated from Lancea ti-betica Hook. f. et Thoms. The structure of lantibeside, C32H42O15, mp 214¡æ, was eluci-dated as 4"-0-[¦Â-D-xylosyl-(1-6)-¦Â-D-glucosyl]-phillygenol by spectral analysis andchemical methods. Pillygenol (2) and oleanic acid (3) were isolated and identifiedalso.
Abstract (Browse 2006)  |  Full Text PDF       
Studies on the Chemical Constituents of Essential oil from the Leaves of Da Ye Fangzang Cinnarnornurn porrectum
Author: Zhu Liang-feng, Lu Bi-yao, Li Yu-jing and Mai Lang-tian
Journal of Integrative Plant Biology 1985 27(4)
    Da Ye Fangzang is a physiological type of containing d-linalool of Cin-namomum porrectum (Roxb) Kosterm. The essential oil in leaves of Da Ye Fangzangcan be extracted by steam distillation with yields of 1.1¨C1.4%. It contained d-linalool95.08% and it can be used in aromatic. We used the methods of GC, GC/MS/DS, IR,and optical ana]ysis, d-linalool and other 29 different chemical constituents were identi-fied.
Abstract (Browse 1922)  |  Full Text PDF       
Isolation of Viable Embryo Sacs and Their Protoplasts of Nicotiana tabacum
Author: Hu Shi-yi, Li Le-gong and Zhu Cheng
Journal of Integrative Plant Biology 1985 27(4)
    Isolation of fixed and fresh embryo sacs has been reported. However,the isolation of protoplasts of embryo sac elements is reported here for the first time.The protoplasts of egg cell, synergids, central cell and antipodal cells have been isolated with the retaining of their viability. Though this is a preliminary work, it indicatesthe potentiality of isolation of naked female gametes of angiosperms, which may beused in genetic manipulation and plant biotechnology. Nicotiana tabacum was grown in the greenhouse of the Department of Biology,Peking University. From opened and unpollinated flowers, the ovaries were removedand sterilized with 70% alcohol. The ovules were dissected out from those ovaries andfollowed by incubation (4¨C8 hrs. 28¡æ) in anenzyme solution containing 2% driselase, 0.65 M mannitol and 0.25% potassium dextran sulfate. Ovules from 3 4 ovariescould be incubated with 1 ml of enzyme solution in a 3 cm petri dish. All these manipulations and the following procedures were carried out under sterile conditions. Afterincubation, ovules were washed 3 times with a washing solution of 0.65 M mannitol.The isolated embryo, sacs and their protoplasts were obtained by gently squashing digested ovules in a small volume of washing solution on a slide. When the fresh ovules were incubated 3¨C3.5 hrs in the enzyme solution, the embryosacs may be successfully isolated in an intact manner, either for mature or immatureembryo sacs. The isolated embryo sac looked plump, viable and very distinct in itsstructure. If the isolated embryo sacs were incubated in 0.01% fluorescein diacetate(FDA) used as a test for the viability of the embryo sac, and observed under fluorescein microscope, the cytoplasm of all embryo sac elements, including egg cell, synergids,central cell and antipodal cells, showed strong fluorescence. It is proved that these iso-lated embryo sacs are still viable. When the incubation of ovules was prolonged as to 8 hrs in certain cases, theboundary wall of the embryo sac may be partially digested and the protoplasts of embryo sac elements came out from micropylar or chalazal end after squashing. The difference of the protoplasts derived from different embryo sac elements could be recognized by their relative size and other characteristics. The egg protoplast is smallerthan that of the synergid. However, the protoplasts of antipodal cells were. obviouslysmaller than that of egg. But the central cell protoplast was the largest among theseprotoplasts and possessed two polar nuclei and a very large central vacuole. All theseisolated protoplasts of embryo sac elements were also proved viable with FDA method. The importance of isolated protoplasts of embryo sac elements is discussed withrespect to genetic manipulations.
Abstract (Browse 2091)  |  Full Text PDF       
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