May 1986, Volume 28 Issue 5


          Research Articles
The Chemical Constituents of Morinda officinalis How
Author: Wang Yan-fang, Wu Zhao-hsa, Zhou Xin-yue, Yie Jing-jian and Qian Qi-tang
Journal of Integrative Plant Biology 1986 28(5)
Abstract (Browse 2180)  |  Full Text PDF       
Enzymatic Synthesis of Proline in Pollen
Author: Sun Chao, Zhu Guang-lian and Cao Zong-xun(T. H. Tsao)
Journal of Integrative Plant Biology 1986 28(5)
Abstract (Browse 1872)  |  Full Text PDF       
Morphological Observation on Pure Cultured Strains from Root Nodules of Elaeagnaceae
Author: Yuan Chang-fang
Journal of Integrative Plant Biology 1986 28(5)
Abstract (Browse 1937)  |  Full Text PDF       
On the Anatomy of Dadoxylon Taiyuanensis
Author: Li Zbong-ming
Journal of Integrative Plant Biology 1986 28(5)
    This paper reports a new species of Dadoxylon Dadoxylon taiyuanensis from the coal balls found near Xiedao village of the Western' Moutain of Taiyuan, Shanxi Province, China. These specimens are well preserved which show the endarch primary xylom and a solid pith, so it suggests that they would be more approximate to conifer woods. In addition, statistical method is used in discription and comparision of the secondary xylem rays.
Abstract (Browse 2306)  |  Full Text PDF       
Middle Jurassic Flora from Shouthern Margin of Eerduosi Basin
Author: Duan Shu-ying, Chen Ye and Niu Mao-lin
Journal of Integrative Plant Biology 1986 28(5)
    This paper deals with some Middle Jurassic fossil plants from southern margin of the Eerduosi Basin. There are 36 species in 13 genus in this flora, i.e. Equisetum multi- dentatus Oishi, E. sp., Coniopteris hymenophylloides (Brongn.) Seward, C. bella Harris, C, burejensis (Zalessky) Seward, C. szeiana Choweet Yeh, C. tatungensis Sze, C. spp., Todites williamsoni (Brongn.) Seward, T. cf. williamsoni (Brongn.) Seward, Cladophle- bis asiatica Chow et Yeh, C. argutula (Heer) Fontaine, C. hsiehiana Sze, C. ( Gleichenites) takeyamae Oishi et Takahasi, C. cf. gracils Sze C. triangularis Oishi, C. grabauiana Pan C. cf punctata (Thoms) Chow et Yeh, C. spp., Baiera cf. concinna (Heer) Kawasaki, B. furcata (L. et H.) Braun, B. sp., Sphenobaiera longifolia (Pomel) Florin, Ginkgo obrutchewi Seward, G. sp., Phoenicopsis angustifolia Heer P. manchurica (Yo- koyama) Yabe, p. sp., Czekanowskia rigida Heer, C. sp., Pterphyllum sp., Elatocladus cf manchurica (Yokoyama) Yabe, E. sp., Podozamites lanceolatus (L. et. H.) Braun P. sp., Carpolithus sp. etc. The dominant groups of this flora are Filicopsida and Ginkgopsida. So it could be compared with the flora of Yanan Formation from inner Earduosi Basin and other floras from Northeast, Northwest and China, such as the floras of Dutung, Mentougon and Dongsheng etc. This flora is different from the Late Triassic flora and the Early Jurassic flora of North China. It belongs to the Coniopteris-Phoenicopsis flo- ra, which was assigned to Early-Middle Jurassic flora by Prof. Sze. But after studying, the authers believe that this flora should belong to Middle Jurassic rather than Early Jurassic. The climate was warm and damp in that time in North China.
Abstract (Browse 2406)  |  Full Text PDF       
A Primary Study about the Relationship Between Nature Stand Production of Picea asperata and ecology environment Condition
Author: Jiang Hong
Journal of Integrative Plant Biology 1986 28(5)
    In this paper, the relationship among nature stand production of Picea asperata forest and ecology environment condition was studied. The nature stand average net- primary-production of P. asperata is 5626kg/ha/a, photo-energy utilize rate (P) is 1.63%, general production (Pg) is 18.85 kcal/m2/d. Spruce tree average net-primary- production (NPP) is 4676 kg/ha/a, P is 1.35%, and Pg is 15.57 kcal/m2/d. The close relationship among ecology environment condition, leaf area index (LAI) and NPP of stand and spruce tree was further studied. It was discovered that among NPP of stand and spruce tree and elevation, slope direction, and stand density are quadric curve correlation; among NPP of stand and spruce tree and soil thickness, LAI is linear correlation, these mathematical model was builded. The poly-factor regression relationship among the NPP of stand and spruce tree and LAI, gradient, elevation, stand density, nomal density, soft thickness, rate of stand grade I tree and slope direction was studied and statistics analysised. In different condition, the photoenerge utilize rate, general production and economy coefficient was further system studied from plant physiology-ecology.
Abstract (Browse 1924)  |  Full Text PDF       
The Chemical Constituents of Duzhong (The Bark of Eucommia ulmoides Oilv.)
Author: Li Dong, Wang Hang-long, Chen Jia-ming and Xu Jun-wen
Journal of Integrative Plant Biology 1986 28(5)
    Seven constituents have been isolated from the bark of Eucommia ulmoides Oliv. Based on spectral analysis and determination of Physico-chemical constants they have been identified as: nonacosane (), n-triacontanol (), -sitosterol (), betulin (), betulic acid (), ursolic acid (), vanillic acid ().
Abstract (Browse 2017)  |  Full Text PDF       
The Chemical Principles from the Female lnftorescences of Typha angustata
Author: Xu Li-feng, Wang Su-xian and Zhu Ting-ru
Journal of Integrative Plant Biology 1986 28(5)
    Seven crystalline compounds have been isolated from the female inflorescences of Typha angustata Bory et Chaub. They were identified as vanillic acid (), E-p-hydroxy- cinnamic acid (), Protocatechuic acid (), E-Pro-penoic acid-3- (4-hydroxyphenyl)- 2,3-dihydrohypropyl ester (), Succinic acid (), p-hydroxybenzaldehyde () and D-mannitol (). () is a new compound. (), (), () and () were attained for the first time from Typhaceae.
Abstract (Browse 2116)  |  Full Text PDF       
Studies on the Constituents of Chinese Traditional Drug Suan Zau Ren
Author: Zeng Lu, Zhang Ru-yi and Wang Xu
Journal of Integrative Plant Biology 1986 28(5)
    Suan Zau Ren (the seeds of Zizyphus spinosus Hu) is a widely used chinese traditional drag for treatment of insomnia, neurasthenia and sometimes for sleepiness caused by physical emaciation in chinese traditional medicine. Its dichloromethane extract afforded four pentacyclic triterpenes of the lup-20(29)-en group, namely betulin (), betulinic acid(), ceanothic acid(), and alphitolic acid (), and one sterol, daucosterol (). Their structures were identified by the spectroscopic analysis. The later three compounds were isolated from Suan Zau Ren for the first time.
Abstract (Browse 2023)  |  Full Text PDF       
A Study on the Chemical Components of the Essentialoi from Peel of Citrus medica L. var. muhensis W. D. et Y.
Author: Wen Ming-zhang, Huang Yuan-zheng, Xiao Shun-chang, Ren Wei-jian, Zhao Hui Yang Shi-jie
Journal of Integrative Plant Biology 1986 28(5)
    The chemical components of the essential oil from peel of Citrus medica L. var. muliensis W. D. et Y. has been studied by means of the GC-MS-DS, the retention index of eapillarly gas chromatography and the Authentic sample addition process. Twenty-nine constituents has been identified from sixty-four seperated peaks. The main constituents is d-limonene (68.2%), geranial (9.5%), neral (5.37%), nerol (2.72%), -ocimen (2.47%) and (+)-Carvone.
Abstract (Browse 2274)  |  Full Text PDF       
Isolation and Identification of the Constituents of Volatiles of Dry Flowers of Aglaia odorata Lour
Author: Wang Tian-song, Huang Ai-jin, Sun Yi-liang, Wu Zhu-ping and Lin Mi-xin
Journal of Integrative Plant Biology 1986 28(5)
    The headspace volatiles of the dry flowers of Aglaia odorata were trapped by a modified circulating adsorption method and were analyzed by GC/MS techniques after desorption by solvent. The components were identified by re, ass spectral and temperature-programmed retention index data on two types of glass capillary columns (OV-101 and PEG-20 M). 54 compounds have been identified from the volatiles, including 21 sesquiterpenes, 8 aldehydes, 7 alkyl benzenes, 6 alkanes, 6 other oxygen-containing compounds and I nitrogen containing compound. 29 compounds have not been reported in the studies on the constituents of the essential oil or absolute oil of the flowers.
Abstract (Browse 1973)  |  Full Text PDF       
The EfFect of B9 upon the Structure of Leaves and Chloroplasts in Peanut plant
Author: Chen Ru-min and Pan Rui-chi
Journal of Integrative Plant Biology 1986 28(5)
    When peanut plants (Arachis hypogaea L.) were treated with aqueous solution of Bo (2000 ppm) by spraying application, the cells of the assimilating tissue were enlarged and the thickness of the leaves were increased. B9 promoted the development of the chloroplast, so that the number of chloroplast, grana and the lamella were increased. As a result, the volume of the container of chlorophyll was enlarged. In addition, the chlorophyll content of file leaves also increased after B9 treatment, there fore the leaves turned dark green.
Abstract (Browse 1871)  |  Full Text PDF       
The Relationship Between Flowering Induction and the Changes of some Phosphatase Activities in Pharbitts nil
Author: Jin Shi-ping, Jin Shu-mei, Cao Guo-yi and Ren Xi-chou
Journal of Integrative Plant Biology 1986 28(5)
    This paper is dealing with the effects of the photoperiodic induction of flowering on the activities of four phosphatases (DNase, RNase, PDase, and 5-NDase) in Pharbitis nil. According to our experimental results, the patterns of changes of activity in these four phosphatases after short-day induction can be put into two cases. One is the case of DNase, which activities are decreased altar short-day treatment both in cotyledons and apices indicating that floral induction leads to dropping of DNA metabolism level Another is the case of the other three phosphatases with a very similar but quite different characteristic from that of DNase. In cotyledons, activities of the three phosphatases after induction are higher than those in the non-induced group. In apices, activities are also higher just after induction, and then drop gradually while activities in the non-induced group rise sharply so that both the two contrast curves show a form of X. These changes are evidently interrelated with RNA metabolic regulation in the apices after floral induction.
Abstract (Browse 1843)  |  Full Text PDF       
The Fine Structural Observation on the Development of Embryo and Endosperm in the Intergene- rfc Crosses of Fadish (Raphanus sativus L.) Cabbageo (Brassica oleracea L.)
Author: Qiu De-bo and D.J. Harberd
Journal of Integrative Plant Biology 1986 28(5)
    The present report deals with the structural abnormalities and abortion of the endosperm, and the related abnormal development of the embryos in the intergeneric crosses of radish (Raphanus sativus L.) with cabbage (Brassica oleracea L.) . The weak development of ER, the occurence of starch grains in chloroplasts, the curious distribution of chloroplasts around the nuclei, and the earlier formation of cellular endosperm are some primary structure abnormalities of endosperm and they may indicate the poor development, low metabolic activities, and precocious growth in the hybrid endosperm. The endosperm abortion, which starts from chalazal end endosperm, comprises the damage of both structure and function of ER membrane, and the disintegration of nuclei and organelles, or even death. The hybrid embryos degenerated very early in 2-cell stage or even zygote stage in case the endosperm Aborted early. In case of endosperm late-aborted, the hybrid embryos grew for quite a long period of time but become slow down as the endosperm showed abnormal development and started to abort. As the results, the embryos were smaller, the embryo propers and the suspensors showed no growth in step with one another, and the embryo cells appeared structural abnormalities, and finally degenerated and aborted.
Abstract (Browse 1907)  |  Full Text PDF       
Studies on 6-benzyladenine Localization in Callus Cells of Tobacco
Author: Sun Jing-san Sun Ching-san, Zhu Zhi-qing Chu Chih-ching, Li Shou-quan and Zhu Ying-min
Journal of Integrative Plant Biology 1986 28(5)
    The localization of 6-benzyladenine in the callus cell of tobacco was studied by means of autoradiography. Electron microscope autoradiographs showed that the silver grains localized at not only cell membrane system such as plasma membrane, tonoplast, plastid membrane and mitochondrion membrane but also nucleus especially nucleolus. The results of the present study indicated that 6-benzyladenine receptor site associated with both cell membrane system and nucleus.
Abstract (Browse 1974)  |  Full Text PDF       
Plant Regeneration from Protoplasts of Brassica Juncea L.
Author: Lu De-yang, Wang Chao, Chen Zheng-hua, Li Wen-bin and Chen Ying
Journal of Integrative Plant Biology 1986 28(5)
    Protoplasts isolated enzymatically from epicotyl and growing tip of Bressica juncea divide to form callus on Kp8 medium. Plant regeneration is obtained from protoplast- derived callus of on MSD3 medium. High concentration of inositol in differentiation medium stimulates plant or shoot regeneration from the epicoty protoplast origin.
Abstract (Browse 1834)  |  Full Text PDF       
The Polymorphy of Fronds and Chromosome Number of Ophioglossum petiolatum Hook
Author: Wang Zhong-ren
Journal of Integrative Plant Biology 1986 28(5)
    A morphological and cytological study of a population of Ophioglossum petiolatum Hook. from the vicinity of Kunming, Yunnan Province was carried out. The fronds of this species are very variabte in. either size or figure. The outlines of laminae of different plants vary from narrowly lanceolate to ovate or broadly ovate (length: breadth can be from 6:1 to 1:1). The apices of laminae range from taper-pointed to acute or blunt, and the bases of laminae from attenuate to cuneate or nearly truncate. In most cases, the spore mother cells of the species have 480 bivalents at pro-meta- phase I of meiosis. The spores produced after normal meiosis are seemingly available. It shows that the species is an octoploid and probably sexual In few plants, many univalents at metaphase I of meiosis have also been seen, but we have failed to count their exact chromosome numbers. However, it reveals that there is a second cytotype in the population. From the fact that O. petiolatum has the great morphological variability and approximately constant chromosome number in a single population, the ecological conditions may not be the main reason of morphological variations of the Species. The high level of polyploidy and probable inbreeding system, the subterranean gametophytes must be hard to outcross, may give it a great ability of genetic lead which increases the genetic heterogeneity. and morphological varibility of populations. The voucher specimens are deposited inthe Herbarium of our Institute (PE). I am grateful to Prof. W. M. Chu of Yunnan University for his helpful suggestion and discuss.
Abstract (Browse 2233)  |  Full Text PDF       
The Crossability of Triticum aestivum with Tetraploid Hordeum bulbosum
Author: Li Da-wei and Hu Qi-de
Journal of Integrative Plant Biology 1986 28(5)
    In order to study the crossability of wheat with H. bulbosum a series of wheat varieties from various sources and their F1 hybrids as well as tetraploid H. bulbosum from different countries were used as parents in this experiment. The main results of the experiment are showed as follows: 1. Twenty-one wheat varieties from Europe, West Asia, America, China, Australia etc. 11 countries and regions respectively as famele parents were crossed with four tetraploid H. bulbosum from Hungary, USSR, Canada and Germany. The seed set percentages in the intergeneric cross combinations ranged from 0.00% to 49.93%. Statistical analysis revealed that there were significant differences between the seed set percentages of wheat varieties. The crossability, with H. bulbosum might differed in different varieties of wheat from same country. 2. Various F1 hybrids resulting from 13 and 26 intervarietal cross combinations of wheat and their parents were crossed with H. bulbosum from Hungary and USSR respectively in different years. The mean seed set percentages of F1 hybrids, their male and female parents were 26.53%, 15.38%, 20.30% and 39.1%, 34.8%, 26.7% respectively. The results indicated that when some wheat varieties having poor-crossability with H. bulbosum were hybridized with other varieties especially with those varieties having high- crossability, the crossability of their F, hybrids probably had higher crossability than their parents having poor-crossability. Six F1 hybrids of wheat obtained from six com- binations of reciprocal crosses, in which the completely non-crossable varieties Hope, Xiao- Bai-Mang and high-crossable varieties Chinese Spring, Fortunate were used as male or female parent alternately, were crossed as female parents with H. bulbosum. All of 6 F1 hybrids were crossable and gave the percentage of seed set from 7.00% to 42.57%, although they ought to carry the dominant Kr genes responsible for non-crossability, which were passed on to F1 hybrids by non-crossable varieties, the parent Hope or Xiao-Bai- Mang. Clearly that is due to the gene interaction between female and male parents. Be- sides Kr loci in wheat, probably other genes can influence the crossability. 3. There were significant differences between clones of H. bulbosum in the crossa- bilities with wheat. In conclusion F1 hybrids of wheat have higher crossability with H. bulbosum than their parents having poor-crossability. This could be advantageous for exploitation of this technique. By selecting even more efficient clones of H. bulbosum and improving embryo culture techniques, the H. bulbosum technique will be used in wheat breeding program- mes probably.
Abstract (Browse 2222)  |  Full Text PDF       
Enzymatic Isolation of Archegonium of Pinus Yunnanensis Fr. and Observations on the Proembryo Development
Author: Liu Cheng-yun
Journal of Integrative Plant Biology 1986 28(5)
    The enzymatic isolation of megagametophyte from fixed and fresh of Pinus yunnanensis Fr. were macerated in a mixture solution of 3% pectinase and 3% cellulase at the 30 for 10C13 hours, and the archegonium has been isolated. Materials were stained with A-S, Feulgen and PAS, and following procedure was cleared in the lactophenol. The ventral canal and egg cell which from division of central cell, the functional sperm nucleus becomes pressed against the egg nucleus, and the new cytoplasm round the zygote nucleus were observated under the microscope of usual light. The four proembryonal nuclei divide simultaneously, resulting in eight nuclei arranged in two tiers of four cells each, and following development were formed wall. In the end, the proembryo of six- teen cells were formed. Two methods on enzymatic and dimethyl sulfoxide with potassium hydroxide to isolate the female sexual cells of gymnosperms have been compared, and several problems are discussed.
Abstract (Browse 1892)  |  Full Text PDF       
The Chemical Constituents of Garctnia xishuanbannanensis Y. H. Li Native to China
Author: Zhong Ji-yu, Wang Wen-duan, Tao Guo-da and Li Kai-lin
Journal of Integrative Plant Biology 1986 28(5)
    Garcinia xishuanbannanensis Y. H. Li is a rare tree species belonging to the Guttiferae and endemic in Xishuangbanna District of Yunnan. From its stem-bark two polyisoprenyl benzophenones-xanthochymol and isoxanthochymol have been isolated. They were identified on the basis of physico-chemical properties and UV, IR, 1HNMR, 13CNMR and MS analysis. The test of antitumor and antibacteria properties of these two compounds in mam- mals will be reported subsequently.
Abstract (Browse 2094)  |  Full Text PDF       
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