March 1987, Volume 29 Issue 3


          Research Articles
Studies on the Chemical Constituents of llligera Rhasiana C. B. Clarke
Author: Yuan A-xing, Qin Liao and Kang Shu-hua
Journal of Integrative Plant Biology 1987 29(3)
Abstract (Browse 2041)  |  Full Text PDF       
Effect of Devernalization on Soluble Protein Component in Winter Wheat Seedling and Subsequent Plant Developmental State
Author: Li Xiu-zhen, Hao Nai-bin and Tan Ke-hui
Journal of Integrative Plant Biology 1987 29(3)
Abstract (Browse 1909)  |  Full Text PDF       
The Influence of Hormones on Microspore Development of Wheat Anther Culture In Vitro
Author: Tian Xiao-ming and Li Qiu-hong
Journal of Integrative Plant Biology 1987 29(3)
Abstract (Browse 1788)  |  Full Text PDF       
Studies on Some Angiospermous Fossil Woods Excavated from the Central Part of HubeiProvince
Author: Qi Guo-fan, Xu Rui-hu Deng Jian-ru and Yang Jia-j邦
Journal of Integrative Plant Biology 1987 29(3)
    Five angiospermous fossil woods of Late Tertiary have been excavated from Xinzhow County, Hubei Province. From the observation of the wood anatomical features they are pre- liminarily identified as Bischofia javanica B1., a species of Leguminosae, and two species of Lauraceae.
Abstract (Browse 1821)  |  Full Text PDF       
The Chemical Elements of Planted Forest of Pinus tabnlaeformis
Author: Chen Ling-zhi Hu Yi-hui, Kong Fan-zhi, Miao You-gui, Ren Ji-kai, Chen Qing-lang and Bao Xian-cheng
Journal of Integrative Plant Biology 1987 29(3)
    The concentration of chemical elements in the edifieator Pinus tabulaeformis of 28- aged pine plantation from leaves, small branches(< 1.5 cm diameter), branches (> 1.5 cm diameter) to trunk graduately declined. C was the most abundant element in pine plantation. The N content was more abundant in leaves, Ca, K, Mg. P were plentiful as well. The mineral content in small root (<2 cm diameter) was richer than that in root (>2 cm diameter). There were plenty of C, N, P. and Ca in the shrub layer, especially in the leaves of shrubs. The concentration of mineral elements in aboveground part of grasses was obviously lower than those in the leaves of shrubs. Comparing chemical elements of litter with those in living leaves and branches, the contents of Fe and A1 in the litter increased, the other elements more or less decreased. The accumulation of C in different layer of plantation except litter reached 22.43 t/ha, the accumulation amounts of Ca and N ranged 157每161 kg/ha. The retention of C in pine plantation was the highest among the elements, Ca retained in tree layer and N, K in shrub layer showed very plentiful also. Ca returned by litter was more than the others except C. The efficiency of P and K uptaked by plant from soil was high. To use the P and K fertilizers will accelerate the growth of Pine plantation.
Abstract (Browse 1949)  |  Full Text PDF       
Essence Constituents of Rosa chinensis Flower
Author: Bu Xin, Huang Ai-jin, Sun Yi-liang, Wu Zhu-ping and Liu Mi-xin
Journal of Integrative Plant Biology 1987 29(3)
    The essence of Rosa chinensis, flower were trapped by a modified circulating adsorption method with XAD-4 as adsorbent and were analyzed by GC and GC/MS techniques. The components were identified by mass spectral and temperature-programmed retention index data on dual glass capillary columns. The major constituents are linalool acetate (14.98%), limonene (12.07%), benzene methyl methoxy (9.88%), citronellol (4.82%), beta-caryophyllene (4.55%), hexanol acetate (3.98%), linalool (3.18%), hexanol (3.17%) etc.
Abstract (Browse 1883)  |  Full Text PDF       
Studies on the Chemical Constituents of Piper hancei Maxim III
Author: Li Shu-ming and Hah Gui-qiu
Journal of Integrative Plant Biology 1987 29(3)
    In continuation with our work on the neolignans of Piper hancei, two new neolignans, hancinone B (ia) and hancinone C (j), with a known compound, crotepoxide, were isolated. The structures of la and j have been identified by spectroscopic and chemical analysis.
Abstract (Browse 2006)  |  Full Text PDF       
A Preliminary Study on Neutral and Acid Inhibitors in the Seed of Kalopanax scptemlobus
Author: Huang Yu-guo
Journal of Integrative Plant Biology 1987 29(3)
    The method of extraction, isolation, purification and identification of the neutral and acid inhibitors in the seeds of Kalopanax Septemlobus was given. It is proved that the neutral inhibitor is coumarin, the acid inhibitor is abscisic acid (ABA) by means of paper chromatograph, thin layer chromatograph, high performance liquid chromatograph (HPLC), color reaction and bioassay. The neutral inhibitor can strongly inhibite not only the seed germination of Brassica Chinensis and the radical elongation, but also that of the seed of Kalopanax septemlobus. The study showed that ABA and coumarin exist in the seed coat, endosperm and pericarp of the Kalopanax Septemlobus seed. Both ABA and coumarin can transport from seed cover (pericarp, seed coat)to the interior (endosperm, embryo) as the seeds were stored in refrigerator. In addition, the different results of extracting neutral inhibitor with water, methanol, and alcohol were compared in this paper.
Abstract (Browse 1904)  |  Full Text PDF       
The Effects of Naphthalene Acetic Acid, Kinetin and Wound on the Callus Formation with Relation to the Biosyntheses of Tryptophan and Indole Acetic Acid in the Cotyledon of Mung Bean
Author: Ma Qing-hu and Cui Cheng (Tsui Cheng)
Journal of Integrative Plant Biology 1987 29(3)
    The effects of the exogenous auxin〞〞NAA, kinetin and wound on the callus formation of the cotyledon of mung bean (Phaseolus radiatus L.) with relation to the biosyntheses of endogeonous tryptophan (Trp) and IAA have been studied. The results indicate that wound plays an important role in the callus formation. The size of wounded surface is directly proportional to the proliferation of the callus tissue. The levels of free Trp and endogenous 1AA decreased in the initial period of the callus formation of mung bean cotyledon but increased in the later period. The substitution of L-Trp for NAA in the culture medium would play almost the same role in the callus proliferation. It has proposed that the exogenous phytohormones affect the biosyntheses of endogenous Trp and IAA, thereafter induce the callus formation. The wound has an important synergism with the phytohormones in this process.
Abstract (Browse 2345)  |  Full Text PDF       
Effect of Auxin, Kinetin and Monosaccharide on Callus Cell wall Composition of Abutilon avicennae Gaertn
Author: Wei Yu-ning and Li Yao-dong
Journal of Integrative Plant Biology 1987 29(3)
    The effects of exogenous kinetin, IAA, glucose (the component of cellulose) and galactose (one of tile inhibiting compounds of cellulose synthesis) on callus cell wall composition of China jute (Abutilon avicennae Gaertn) were investigated. When the China jute callus materials were treated with 2 mg/l IAA and H3-glucose, there was a 437% increase of the Ha-glucose, as compared with control. When galactose was added simultaneously with H3-glucose, there was a 138% increase of the H3-glucose as compared with control. Galactose inhibited H3-glucose incorporate content in cellulose, and also in pectin and hemicellulose. In the experiment with 10 mg/l kinetin treatment of the callus materials, when galactose was added, the incorporation H3-glucose was inhibited. It is interesting that the kinetin enhanced H3-galactose incorporation into all cell wall components. When it was added simultaneously with glucose, glucose inhibited H3-galactose into the cell Wall components. Results showed that the hormones effect cell wall component, not only concerned with hormones, but also concerned with exogenous monosaccharides. When exogenous monosacchrides were added to the culture medium (as galactose, glucose) in combination or respectively, they are quite different in effecting hormone induced China jute callus cell wall. When the callus was treated by IAA, galactose inhibited H3-glucose incorporation into cell wall components. When it was treated by kinetin, glucose inhibited H3-galactose incorporation into cell wall components.
Abstract (Browse 1948)  |  Full Text PDF       
The Effect of Low Temperature on Superoxide Dismutase in Hybrid Rice and Their Parental Lines
Author: Liu Hong-xian, Zeng Shao-xi, Wang Yi-rou, Li Ping, Chen De-feng and Guo Jun-yan Kuo Chun-yen
Journal of Integrative Plant Biology 1987 29(3)
    The hybrid rice and their parental lines were exposed to the low temperature of 0, 每2, 每4⊥ for 16 hours. The activities and isoenzymic patterns of SOD in various organelles of rice seedlings were studied. The experimental results are as follows: 1. This enzyme was located mainly in the cytosol (96%), the rest in mitochondria (2%) and chloroplasts (2%). The electrophoretogram showed that there were four isoenzymic activity bands of SOD in cytosol and mitochondria, three bands in chloroplasts. One band with relatively slow migrating rate was Mn-SOD isoenzyme and others were Cu-Zn SOD isoenzymes. 2. The change of SOD activities was obvious in the plant pretreated at 每2⊥ and 每4⊥ temperature for 16 hours. The effect of low temperature on SOD in various organelles was different. The sensitivity of SOD in chloroplasts was higher than those in mitochondria and cytosol. The two activity bands of Cu-Zn SOD isoenzyme in chloroplasts and mitochondria also decreased at low temperature. 3. The response of SOD activities in chloroplasts from hybrid and parent of rice to low temperature showed that the cold tolerance of hybrid progeny was similar to that of the material lines.
Abstract (Browse 1853)  |  Full Text PDF       
Polyacrylamide Gel Electro rhoresis Analysis of Actin in Pollen Mother Cells of Higher Plants
Author: Wang Xin-yu
Journal of Integrative Plant Biology 1987 29(3)
    The existence and change of actin in the pollen mother cells of Secale cereale L., Vicia faba L., and Lilium davidii var willmottiae were identified by the application of the polyacrylamide gel electronphoresis. It was proved that the pollen mother cells of the three plants contained certain amount of actin at cytomixis stage and that this protein began to appear at early zygotene stage and to disappear after tetrad stage. Finally, the relation between the actin and cytomixis was discussed.
Abstract (Browse 1912)  |  Full Text PDF       
Investigation on Fertilization of Cephalotaxus
Author: Chen Zu-keng, Li Ying and Wang Fu-xiong Wang Fu-hsiung
Journal of Integrative Plant Biology 1987 29(3)
    The structure of spermatogenous cell of Cephalotaxus is unique among the gymnosperms. While towards the mature stage, its nucleus is close to one side of the spermatogenous cell, and on the other side there is abundant and prominent . cytoplasm, which contain a group of the aggregate cytoplasms of radial arrangement similar to blepharoplast of spermatogenous cell of Ginkgo. But, there are two opposite blepharoplasts at either side of the nucleus in Ginkgo, and while there is only one blephareplast at one side in Cephalotaxus. This is one feature of the sexual process in Cephalotaxus. When the pollen tubes approach the top of the archegonia, the division of the spermatogenous cell takes place and there are two almost similar sperm cells both in size and morphology. It is interesting to note that the cytoplasm of the sperm cell contains certain granules of nucleolus-like structure, which appears to be a rare phenomenon among the gymnosperms. This is another feature of the sexual process in Cephalotaxus. These two features are the important characters of Cephalotaxaceae. The egg morphology of Cephalotaxus is also unique among the conifers, its outline looks like a carrot. The upper part of the egg is rather wide and is about 85 to 108 米m in width. On the other hand, the opposite end is gradually becoming narrow and about 910 to 1100 米m in total length. So the ratio of the length and width in the egg of Cephalotaxus is about 10:1. The structure of the egg in Celhalotaxus fortunei and C. oliveri have the following common feature: 1. When their eggs mature the cytoplasm of the egg at lower part of the nucleus possesses deep- staining and fine granules of 2 to 3 groups of aggregate cytoplasm. 2. During maturation of the egg, some of the granules of nucleolus-like structure are scattered in the cytoplasm. As fer- tilization takes place the number of these granules reaches the peak. This condition has been encountered in the egg of Amentotaxus argotaenia. Therefore we could conclude that they are closely related between Cephloraxaceae and Taxaceae. The fertilization of Cephalotaxus fortunei occured on May 10 to 24 (1983), and that of C. oliveri took place on May 28 to June 13 (1983). The fertilization of the genus belong to the type of undergoing mitosis prior to complete fuse of both male and female nuclei. This type of fertilization has been found only in Pinaceae and Cephalotaxaceae. After fertilization the structure of fertilized egg becomes prominent in polar organization. In other words, the cytop- lasm at upper part of the fertilized egg becomes highly vacuolated and that at lower portion, conversely, is rich in abundant proteinous vacuoles and certain granules of nucleolus-like structure dispersed in the cytoplasm. Because the division and differentiation of the proembryo are proceeding at the base of the archegonium, the large inclusions and the nucleolus-like granules may be involved in the nourishing and development of the proembryo.
Abstract (Browse 2067)  |  Full Text PDF       
Plantlets Regenerated from Callus Culture of Narcissue tazetta
Author: Gu Heng-sen, Gao Cui-hua and Wang Shu-fen
Journal of Integrative Plant Biology 1987 29(3)
Abstract (Browse 1740)  |  Full Text PDF       
The Dominant Species of the Dwarfing Mossy Forest on the Summit of Mt. Ailao in Jingdong Yunnan Lithocarpus pachy Phylloides
Author: Xie Shou-chang
Journal of Integrative Plant Biology 1987 29(3)
Abstract (Browse 2055)  |  Full Text PDF       
A Study on the Triglycerides Composition in Oils of Canarium pimela Leenh
Author: Ling Xue-kun and Chen Wen-xiang
Journal of Integrative Plant Biology 1987 29(3)
Abstract (Browse 1857)  |  Full Text PDF       
The Relationship Between the Intercellular Chromatin Migration of Pollen Mother Cells and the Changes of Chromosome Numbers During the Genesis of Male Gametes in Allium Cepa
Author: Wang Chong-ying and Zheng Guo-chang
Journal of Integrative Plant Biology 1987 29(3)
    During the genesis of microspores and male gametes, the percentages of cells with an increas- ed and decreased chromosome number at metaphase j, mitotic metaphase of microspores and generative cells approximated to the one at previous stage (metaphase i, metaphase Il, and microspores) respectively, and the one at metaphase 1 again approximated to the percentages of chromatin intercellular migration of type 2.3↙1 and 1↙2.3 respectively, which had no significant diversity statistically. According to this fact, it was considered that the changes of chromosome numbers were related to the chromatin intercellular migration at synizesis stage and that pollen mother cells with an abnormal chromosome number at metaphase i basically were able to continue developing untill the formation of male gametes.
Abstract (Browse 1916)  |  Full Text PDF       
Somatic Embryogenesis from Sugarcane Protoplasts
Author: Yan Qiu-sheng, Zhang Xue-qin and Gu Ming-guang
Journal of Integrative Plant Biology 1987 29(3)
    Calli derived from leaf explant of sugarcane F134 pollen plant were used as the material for isolating protoplasts. Protoplasts were cultured in modified MS medium by means of liquid-thin layer culture. The first cell division of protoplasts was observed within a week in culture. Calli were formed about 5每6 weeks after culture. Selected calli consisting of embryonic cell masses were transferred onto differentiation medium without 2,4-D or with lower concentration of 2,4-D, but supplemented with BA. About 2每3 weeks, coleoptiles have been developed in some calli, radicls or roots were differentiated in some others. The process of somatic embryogenesis from protoplasts traced systematically from the first division to the formation of calli.
Abstract (Browse 1852)  |  Full Text PDF       
Floral Morphology of Populus lasiocarpa Oliv and Its Phylogenetic Position in Populus
Author: Hong Tao, Ma Zuo-li and Chen Jing-shi
Journal of Integrative Plant Biology 1987 29(3)
    The results of this study, which involved macroscopic, microscopic and SEM investigation of flowers of Populus lasiocarpa Oliv., may be stated as follows: 1. Female, male and bisexual flowers possess well developed perianth greenish in color with obvious veins and glandular teeth. The branched vascular bundles and stomata apparatus are observed in the transection of the perianth. It suggests that the perianth is homologous with the leaf. 2. Each pistil of female and bisexual flowere possesses 3(每5) separate styles. The ovary is I-celled with 16每34 ovules. Each male flower has 41每110 stamens. The connective of stamen is protrusive. Presence of bisexual, polygamous flower and monoecism is not rare. 3. In comparison with several other species of Populus, P. lasiocarpa is supposed to be the most ancient and primitive species of the Populus. The central and south-west China perhaps is one of distributional center of Populus.
Abstract (Browse 2576)  |  Full Text PDF       
Observation on the Sexual Reproduction of Peridinium bipes Stein (Dinophyceae)
Author: Liu Zhi-li and Zhang Shu-qun
Journal of Integrative Plant Biology 1987 29(3)
    Sexual reproduction of the dinoflagellate Peridinium bipes Stein was observed. At the late growth season (from late March to early April), the small, unarmored motile cells i.e. gametes are produced by division of the cate cell. Isogamy occurs in this species. Two gametes in fusion are morphologically indistinguishable, but their behavior are different. Before fusion, two gametes are connected by a transparent granular structure and move quickly for about ten minutes. When the plasmogamy almost completes, the fusing cells stop moving for a while and the transverse flagellum of one gamete is cast off. By staining with modified carbol fuchsin, it was proved that the karyogamy takes place soon after plasmogamy and the change of chromosomes in this period was also observed. The zygote keeps motile for about 14 days before casts off its two flagella and becomes aplanozygote. During this period i.e. planozygote stage, the zygote enlarges from 55 ℅ 50 米m to 75 ℅70 米m, intercolary bands connecting thecai plates widen, lots of oil droplets are produced as storage granules. After sinking to the bottom of flask, aplano- zygote continues changing: exospore wall is cast off, mesospore wall and endospore wall are thickened, oil droplets turn to starch grains, protoplast contracts and becomes spherical, a large red lipid granule, perhaps eye-spot, appears. Afterward, aplanozygote has become hypnozygote i.e. resting cyst. The type of sexual reproduction, the amphiesma of the zygote, the resistance of hypnozygotic wall to acid and alkali, the relationship between fossil dinoflagellate remain and the wall of hypnozygote were discussed. It was also considered that the three formas of P. bipes named by Huber-Pestalozzi were the different stages of zygote development.
Abstract (Browse 2346)  |  Full Text PDF       


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