February 1988, Volume 30 Issue 2


          Research Articles
A study on the Chemical Constituents of the Essential oil from Dendranthema indicum (L.) Des Moul
Author: Lin Zheng-kui and Hua Ying-fang
Journal of Integrative Plant Biology 1988 30(2)
Abstract (Browse 1948)  |  Full Text PDF       
Peroxidase Isozymes of Saccharum, Arundinaceum and Their Hybrids
Author: Liao Zhao-zhou, Tang Ming-de and Jin Yu-feng
Journal of Integrative Plant Biology 1988 30(2)
Abstract (Browse 1877)  |  Full Text PDF       
An Abnormal Embryo Sac in Eriospermu luteorulerum Baker
Author: Lu An-ming
Journal of Integrative Plant Biology 1988 30(2)
Abstract (Browse 1799)  |  Full Text PDF       
Observation on Enzymatically Isolated-embryo Sacs in Adenophora axiIliflora: Fertilization,Zygote and Endosperm
Author: Wu Yan and Zhou Chang
Journal of Integrative Plant Biology 1988 30(2)
Abstract (Browse 1817)  |  Full Text PDF       
Ultrastructural Change in Sesbania Cann Abina Root Nodules During Their Developments
Author: Jing Yu-xiang and Zhang Bao-tian
Journal of Integrative Plant Biology 1988 30(2)
Abstract (Browse 1959)  |  Full Text PDF       
A Preliminary Study on Light-photosynthetic Characteristic in the Leaves at Various Age for Aneurolepidium Chinense
Author: Du Zhan-chi and Yang Zong-gui
Journal of Integrative Plant Biology 1988 30(2)
    In June and July, net photosynthetic rate and its light intensity coefficient, as well as light saturation point, near saturation point and half saturation point are the highest during sevaral days after fully expanded leaf; namely, in uppermost expanded leaf for Aneurolepidium chinense. Aforesaid characteristic indexes of younger leaves and older leaves are lower. The difference of net photosynthetic rate or light intensity coefficient Of photosynthesis in 'the leaves of various age with falling light intensify reduces gradually. Light compensation point in halfunfolded young leaf is lower, then increased gradually with the ageing leaf. The prime-leaves play the major role in photosynthetic production due to the strong capability of light utilization. The young leaves have the ability to utilize weak light and the old leaves are more tolerant to shade.
Abstract (Browse 1942)  |  Full Text PDF       
Chemical Constituents of Glycyrrhiza inflata Bat
Author: Yang Shi-lin and Liu Yong-long
Journal of Integrative Plant Biology 1988 30(2)
    There are six species of the genus Glycyrrhiza, growing in China. Along with G. uralensis, the species G. inflata is one of the widely distributed in Gansu province and Xinjang autonomous region. This species has been used in medical practice for a long time and recorded in the Chinese Pharmacopoeia of 1977 edition. The chemical constituents of G. inflata has not been reported in detail. This paper reports the isolation and identification of twelve compounds including eight flavonoids, three triterpenoids and a phytosterol from the roots of G. inflata gathered from Jinta county, Gansu province. On the basis of chemical and spectroscopic data, their structures were identified as licocha-lcone A and B, licoflavone, liquiritin, liquiritigenin, isoliquiritigenin, 4,7-dihydrox-yflavone, ononin, glycyrrhizic acid, glycyrrhetinic acid, ll-deoxyglycyrrhetinic acid and -sitosterol respectively. Licochalcone A was the major component among the flavonoids. It's 13C NMR data were recorded and their assignments were given for first time.The 4,7-dihydroxyflavone and ononin were isolated from the genus Glycyrrhiza for the first time.
Abstract (Browse 2615)  |  Full Text PDF       
Effects of Abscisic Acid in Senescence of Garlic Al1tium sativura Scape
Author: Wang Wen-hong, Tan Zhi-yi and Ji Song-yang
Journal of Integrative Plant Biology 1988 30(2)
    Removing the apical cloves from the excised garlic scapes could delay the senescence of scape and decrease the rate of dry matter loss during storage. The ABA content of all the portions of the scapes with apical cloves is decreased as the prolongation of storage period; but its content of all the portions of the scapes with the cloves reaches their peak one after another within the first 6 days of the experiment period. The peak of ABA in the apical cloves appears earliest; the ABA content of any portion of the scapes is lower than that of the cloves, and gradually decreases from upper to lower portions, and the peak of ABA content appears in the sequence as above. Exogenous ABA which is added to the top of the scapes without cloves would simulate the function of the apical cloves in part and accelerates the chlorophyll destruction and senescence of the scapes. No ethylene can be detected by the G. C., in the process of senescence. The authors suggest that ABA is mainly synthesized in the cloves, and then transported into the scapes from there. In the senescence of garlic scapes and in the redistribution of material between the apical cloves and the scapes (the relation between the sink and source), ABA functions as transporting information and promoting senescence but ethylene has not such a function. The senescence of garlic scape could be divided into two stages: first the static phase and second, the active phase.
Abstract (Browse 2229)  |  Full Text PDF       
Study on the Esterase Isozyme from the Related Citrus Plants
Author: Liao Ying-fen
Journal of Integrative Plant Biology 1988 30(2)
    In this experiment, the polyacrylamide gel electrophoresis is adopted to test the esterase isozyme of seeds and seedling of the fourteen species of related Citrus plants. The relationship with each other is analysed according to the resutls of zymogram and scanning chart. The data show that there are differences of various degrees in the number of the isozyme bands, the enzyme activity, the migration of enzymes and their chromatogram scanning. The isozyme of the same species has different forms of expression in the different period of development. The esterase isozyme zymogram of the Citrus seed is generally stable. It can be used as the biochemical and genetical index to detemine the genetic ralationship of Citrus.
Abstract (Browse 1934)  |  Full Text PDF       
Damage of PSII by Sulfite
Author: Liu Rong-kun and Hiroshi Inoue
Journal of Integrative Plant Biology 1988 30(2)
    The photoreduction of DCIP by PS pasticles isolated from spinach leaves was inhibited by sulfite and the degree of inhibition was increased with the increase of sulfite concentration. The site of sulfite damage was on the oxidation side. In dark, electron flow from H2O to DCIP and from DPC to DCIP was not affected by sulfite. With certain concentrations of sulfite, the damage to PS particle varied with time of sulfite treatment and the mechanism of the damage might be related to the discretion of 33 kD polypeptide from thylakoid membrane and the leakage of Mn. Sulfite did not specifically damage the newly prepared thyla- koid, but this was the case with aged thylakoid. The rate of DCIP photoreduction decreased as the aging process was prolonged. Decrease in Mn content correlated with the decrease of DCIP photoreduction. Especially in the presence of EDTA, with the decrease of Mn, the rate of electron transport was severely reduced.
Abstract (Browse 1878)  |  Full Text PDF       
Composition and Distribution of Phospholipids in Hevea Latex Lipids
Author: Bao Feng, Xu Yong-rui, Zhao Qin-xiu and Liu Zu-tang
Journal of Integrative Plant Biology 1988 30(2)
    In this paper qualitative analyses of the phospholipid components of Hevea latex of two asexual progeny Hongxing No. 1 (wind-tolerant species) and the 93-110 (chill-tolerant species) were carried out by using one dimensional thin-layer chromatography. Eight phospholipid components were separated, and six of them were detected, the two among' them are phospholipid components never detected before. Besides, authors have conducted rapid quntitative analysis of the distribution of various phospholipid components in total lipid, rubber phase and lower layer of Hevea latex by thin- layer chromatographic scanning and the corrective method of absorbance proportional coefficint.
Abstract (Browse 2239)  |  Full Text PDF       
Ultrastructure of Chloroplast and Photosynthetic Properties of the Leaves at Different Position in Maize
Author: Wang Qun-ying and Hu Chang-hao
Journal of Integrative Plant Biology 1988 30(2)
    The leaves at the different position, the basal, the middle and the upper, were examined with TEM. The content of chlorophyll and photosynthetic rate in those leaves was measured. The ultrastructure of chloroplast varies with the different position of leaves. The amount of grana lamellae, stroma lamellae and stroma increased generally with the rising position of leaves. That of the ear leaf at the middle position, was the highest than that of the others. In the upper leaf, the amount of grana lamellae, stroma lamellae and stroma was reduced. Also, the content of chlorophyll and the photosynthetic rate in the ear leaf was higher as compared with that is the other positional leaves. The observations revealed that the tendency of the variation in ultrastructure of the leaves at different position was in conformity with photosynthetic properties. The ultrastructure and function of ear leaf were better than that of other leaves.
Abstract (Browse 2121)  |  Full Text PDF       
DNA Synthesis in Microspores in Anther Culture of Wheat (Triticum aestivum L.)
Author: Zeng Jun-zhi
Journal of Integrative Plant Biology 1988 30(2)
    Anthers with mid-unlnucleate microspores were cultured on W5 medium supplemented with 0.5 mg/l kinetin, 2 mg/l 2,4-D and 9% or 3% sucrose. At a series of interval (0, 1, 1.5 2 14 days) after cultured, the anthers were labelled with 3H-thymidine (4 MCi/mi) for 24 h, fixed, and then performed autoradiography according to conventional method. Results show that after cultured for 24 h, 3H-thymidine was incorporated into some late-uninucleate microspores (see Plate I, 3), and after for 2.5 days, vegetative nuclei in pollen grains were la- belled (see Plate I, 4). Usually, vegetative nuclei were labelled frequently and generative ones were labelled rarely. Sometimes generative cell which could synthesis DNA might develop suspensor-like structure individually (see Plate I, 13). During early stage of development of a multicellular pollen grain, the DNA synthesis in the cells were synchronized. With pollen development, the synchronism of DNA synthesis was destroyed. When anthers cultured on medium with 3% sucrose, DNA in microspores could be synthesized normally, and the number of labelled microspores was more than that of anthers cultured on medium with 9% sucrose. However, on medium with 3% sucrose, the nuclei in microspores stopped dividing after one or two divisions and the cell wall of them could not be formed and multicellular pollen was not observed. It seems that the absence of multicellular pollen on medium with 3% sucrose was primarily due to the block of cell division and cell wall formation, not due to the interruption of DNA synthesis.
Abstract (Browse 2143)  |  Full Text PDF       
Study on the Somatic Embryogenesis from leaf of Begonia fimbristipula Hance in vitro
Author: Zhang Lan-ying, Li Geng-guang and Guo Jun-Yan
Journal of Integrative Plant Biology 1988 30(2)
    Liquid SH medium supplemented with 0.125 mg/l 2,4-D, 0.25C0.50 mg/l BA, and 10% v/v CM or 0.2 w/v CH, incubating on the shaker was favourable to directly initiating normal development and large number of somatic embryoids from leaf of Begonia fambristipula Hance in vitro. Globular, hearttorpedo and cotyledon shaped embryoids appeared in the same medium after incubation for 50 days. Although somatic embryoids and adventitious buds also originated from the epidermal cells of the leaf, there were some differences between them: The development of somatic embryoid involved four different periods, but the adventitions buds had none; Globular embryoid would fall away from explant and distributed in the medium, but buds would remain tightly connected with explant throughout; torpedocotyledon embryoids have the specific structures with shoot and root ends, two cotyledons with the middle apical meristem and a procambium, but the adventitious buds have the shoot apex only.
Abstract (Browse 2134)  |  Full Text PDF       
Ultrastructural Studies of Vegetative Cells on Jaoa bullata (Jao) Fan
Author: Wang Mo-shan, Su Wen-tuan, Wang Xiao-ming and Liang Jia-ji
Journal of Integrative Plant Biology 1988 30(2)
    The present study reports the ultrastructural features of the vegetative cells in Jaoa bullata (Jao), Fan., an endemic species of Chlorophyta in China. Its thallus is composed of 3 layers of cells. Outer cells are the smallest in size, containing abundant cytoplasm, rich in various organelles; their vacuoles are smaller. The cells of mid-layer possess a large central vacuole. Their cytoplasm becomes a thin layer appressed against the cell wall; Various kinds of the organelles are still clearly visible. Inner ceils are extremely vacuolated. Their cytoplasm looks like degenerated. The cells are uninucleate, each containing a parietal, laminate chloroplast with numerous pores, which cause chloroplast a net-like appearance. The photosynthetic lamellae in a chloroplast include a number of thylakoids mainly in pairs. Chloroplast contains several pyrenoids, which are penetrated by 1 or 2 thylakoids. The present study deals with the structural characteristics of the mitochondria, plastids, endoplasmic reticulum and dictyosomes in the cells of Jaoa bullata. Two groups of intranuclear microtubules are present in a cell. Based on the similarity to Ulva mutabilis in the thylakoid arrangement and the ultrastructural features of the pyrenoids, authors suggest that daoa bullata (Jao) Fan may be closely related to Ulvaceae, among the advanced taxa in the evolution of Chlorophyta.
Abstract (Browse 2339)  |  Full Text PDF       
Electron Microscopic Observation on the Infected Cells During Pea (Pisum sativum L.) Nodule Senescence
Author: Han Shah-hua and A. F. Yang
Journal of Integrative Plant Biology 1988 30(2)
    Ultrastructural changes of the infected cells have been observed by transmission electron microscopy during pea root nodule senescence. The infected cells and bacteroids of pea nodules ultimately senesce, their senescence has certain laws and features. Firstly, peribacteroid membrane were loosened, leaving a large electron-empty space with fibrillar and vesicular material. Then bacter0id cytoplasm lost features and aggregated into some clustered electron- dense material. At next stage bacteroids were structurally emtpy and appeared like ghost cells. Companying bacteroid senescence, host cytoplasm changed from dark to light in electron density and cell organelles gradually decreased. After the host cell tonoplasts and plasmalemma broke down, the infected cells showed a chaotic state of bacteroids and host cell debrises. Finally, infected cells disintegrated completely. Sometimes some young bacteria were seen in the intercellular spaces surrounded by degenerating cells, in the degenerating cytoplasm. A few infection threads were also found among the disintegrated bacteroids, even some of them were releasing the bacteria into the degenerating host cytoplasm.
Abstract (Browse 2010)  |  Full Text PDF       
The Ultrastructure of Mature Embryo Sac in Dolichos lablab
Author: Chen Yi-ming and Wang Fu-xiong(Wang Fu-hsiung)
Journal of Integrative Plant Biology 1988 30(2)
    The ultrastructure of the egg and the central cell in Dolichos lablab was investigated. When the embryo sac is mature, cell wall is formed only at the micropylar end of the egg and the synergids. At the chalazal end, no cell wall exists between the egg and the synergid, bet- ween the egg and the central coll, between the synergid and the central cell, and the plasma membranes of the adjacent cells are very close to each other. In some regions between the plasma membrane of the egg and that of the central cell, some moderate electron dense substances are present. Many mitochondria and plastids are present in the egg cytoplasm, but ER and dictyosomes are few. At the micropylar end of the synergid an elaborate filiform appara- tus is distinct, and many ER tubules are dispersed in the cytoplasm of the micropylar end. It shows that the synergid would have a secretory function. The synergid contains numerous rough ER in the cytoplasm at the chalazal end, and between the plasma membrane of the egg and that of the synergid there are some vesiculae, indicating active metabolite transport betwe- en two cells. The central cell displays a high metabolic activity since numerous mitochondria, dictyosomes and ER are present in its cytoplasm. Its cell wall ingrowths form a lot of finger-like projections penetrating into the cytoplasm, which displays the feature of the transfer cell. There are many lipid bodies in the peripheral, cytoplasm. It is suggested that the central cell would play an important role in the nutrition of the embryo sac.
Abstract (Browse 2367)  |  Full Text PDF       
On the Application of the Analysis ct Variance in the Identification of Dadoxylon woods
Author: Li Zhong-ming
Journal of Integrative Plant Biology 1988 30(2)
    This paper suggests that the analysis of variance could be used to the distinction of Da- doxylon-like woods and some quantitave characters of Dadoxylon taiyuanensis, Amyelon radicans, A. xui, A. equivius and Billigea resinosa, for example, diameter of ray cells and tracheids, height of rays, and diameter ratio of ray to tracheid, are compared and discussed. The comparision and discussion show quantitave character is more useful to identification of fossil plants than others if analysis Of variance is used.
Abstract (Browse 1914)  |  Full Text PDF       
The Study on the Constituents of leaf Essential Oils in Citrus limonia Osbeck
Author: Chu Jian-qing, Zhang Zheng-ju and Pu Fan
Journal of Integrative Plant Biology 1988 30(2)
Abstract (Browse 1951)  |  Full Text PDF       
Preliminary Analysis on Chemical Constituents of Essential Oil from Inflorescence of Artemisia Annua L.
Author: Liu Qun, Yang Zhi-yun, Deng Zhi-bang, Sa Gou-heng and Wang Xin-fu
Journal of Integrative Plant Biology 1988 30(2)
Abstract (Browse 1971)  |  Full Text PDF       
Studies on the Alkaloids of Alseodaphne hainanensis Merr
Author: Zhang Fen-xian, Liu Mei-fang, Li Yu-jing and Mia Lang-tian
Journal of Integrative Plant Biology 1988 30(2)
    Six alkaloids were isolated from the stem of Alseodaphne hainanensis Merr. (Lauraceae) collected in Hainan Island. Four of them were identified as xylopinine, armepavine, doryafranine, and 1-(4-methoxybenzyl)-6,7-methylenedioxy-l,2,3,4-tetrahydroisoquinoline by means of spectral analysis and comparison of physico-chemical constants.
Abstract (Browse 2240)  |  Full Text PDF       
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