April 1989, Volume 31 Issue 4

 

          Research Articles
A Study on Nuclear Inclusion in the Cells of Flowers and Leaves of Catalpa
Author: Huang Jin-sheng, Fan Ru-wen and Jiang Li
Journal of Integrative Plant Biology 1989 31(4)
      
    
Abstract (Browse 1793)  |  Full Text PDF       
A Study on Chemical Constituents of the Essential Oil from Mosla scabra (Thunb.) C. Y. Wu et H. W. Li
Author: Lin Zheng-kui and Hua Ying-fang
Journal of Integrative Plant Biology 1989 31(4)
      
    
Abstract (Browse 1937)  |  Full Text PDF       
A Preliminary Study on the Seasonal Change of Photosynthetic Rate in Stipa grandis Community
Author: Jin Qi-hong, Sheng Xiu-wu, Qi Qiu-hui and Hong Liang
Journal of Integrative Plant Biology 1989 31(4)
      
    
Abstract (Browse 1762)  |  Full Text PDF       
The Seasonal Dynamics of the Biomass of Vitex Shrubland in Mountainous Area of Huairou County in Beiiing
Author: Dai Xiao-bing
Journal of Integrative Plant Biology 1989 31(4)
      
    Vitex shrubland is a typical secondary shrubland in mountainous area of Huairou County and also in the low-mountainous area of north China. Over 80% of the biomass of the shrubland are contributed by its dominant species-Vitex negundo var. heterophylla. Due to frequent cutting, the aboveground biomass of Vitex shrubland was continually removed, and that of its underground accounts for 55% of the total biomass. In 1986, the primary net production of Vitex was 6363.6 kg﹞ha-1﹞a-1, in which 72.4% came from its roots, only 12.4% was contributed by the stems. The highest growth rate of the roots of Vitex appeared from May to July and the average productivity in this period was 49.7 kg﹞ha-1﹞d-1. The highest growth rate of stems with the average of 8.69 kg﹞ha-1﹞d-1 was found from July to October. Obviously, the photosynthetic products had firstly transported to the roots and were accumulated, and then transported to the stems. The allocation of photosynthetic products in the different parts of shrub organ showed that photosynthetic products were accumulated in ball root, then transport to other parts of roots. The top of stems was the first part where photosynthetic products were accumulated, then other parts of stems. A comparison with the biomass of other Vitex shrublands in the similar enviroment in Beijing area shows that biomass of the shrublands in north slope is larger than that in south slope. Comparing the biomass of Vitex shrubland with the biomasses of artificial forests in the same area with similar habitat we found that trees in the site occupied by the shrubs which we studied, can offer 5 times biomass than that of the shrubs.
Abstract (Browse 1892)  |  Full Text PDF       
A New Understanding of the Bohaidinioid Dinoflagellates (Cont.)
Author: Xu Jin-li and Mao Shao-zhi
Journal of Integrative Plant Biology 1989 31(4)
      
    After the first dinoflagellates monograph was published in China in 1978, the new information about the Bobaidinioid group has been accumulated, which results in reviewing our understanding of this group. The main emendations here emphasize the combination archeopyle of tAa+3Ia or tAa+3I type, the number and distribution of girdle projections, and the nature of girdle folds of this group, on which the systematic position of Bohaidinioideae belonging to Deflandreaceae of Peridiniineae has been allocated. Five forms of seven species of four genera have been emended, and two new species and three new forms have also been described in this paper. They are Bohaidina alveolae, B. spinosa form spinosa, B. spinosa form quadrata, and B. spinosa form fusiforma. In addition an attemp has been made of reconstructing the paleoecology of Bohaidinioideae and its possible role in the process of dinoflagellates migration from marine to freshwater, based on reviewing the geological history of nonmarine dinoflagellate records in the world, the occurrence of Bohaidinioideae and other associated genera and species in the same stratum, combined with available geological and palaeontological evidences.
Abstract (Browse 1934)  |  Full Text PDF       
Studies on the Alkaloids of Stephania yunnanensis
Author: Chen Yan, Fang Sheng-ding, Liang Dong and Jiang Fu-yin
Journal of Integrative Plant Biology 1989 31(4)
      
    Ten known alkaloids, sinoacutine (i), 1-tetrahydropalmatine (j), d-stepharine (k), 1- stepholidine (l), 1-corydalmine (m), stephanine (n), palmatine (o), dehydrocorydalmine (p), stepharanine (q) and roemerine (r) were isolated from the roots of Stephamia yunnanensis H. S. Lo. Their structures were determined from spectral data and chemical evidence.
Abstract (Browse 2079)  |  Full Text PDF       
Studies on the Chemical Composition of the Head Space and Essential Oil of Fresh Flower of Ku-Shui rose (Rosa setate X R. rugosa)
Author: Xue Dun-yuan, Chen Ning, Li Zhao-lin and Chen Yao-zu
Journal of Integrative Plant Biology 1989 31(4)
      
    The chemical composition of the head space and essential oil of fresh flowers of KU-Shui rose, which were collected by adsorption with Paropark Q (60每80 mesh) and steam distillation-extraction respectively, have been identified both by capillary gas chromatography/mass spectrometry and the Kovats Index of the components. From the head space, 63 compounds have been identified, among which 27 compounds were not present or found only in trace amount in the essential oil. From the essential oil, 79 compounds have been identified, among which 55 compounds have not been found in this flower previously.
Abstract (Browse 2008)  |  Full Text PDF       
Direct Budding from Explants in Young Panicle Culture of Some Monocotyls
Author: Ling Ding-hou, Chen Mei-fang, Chen Wan-ying and Ma Zhen-rong
Journal of Integrative Plant Biology 1989 31(4)
      
    In the young inflorescence (panicle) culture of 22 species of 6 genera, i.e. Oryza, Echino- chloa, Pennisetum, Setaria, Panicum and Musa, it was found that the young inflorescences budded directly from explants in 19 species of all 6 genera in the culture medium containing NAA and kinetin, but no 2,4-D. According to the frequency and the speed of direct spikelet budding (DSB), these species could be classified into four groups (Table l). The response of the young panicles in the first group (Cultivar rice, O. spontanea, Echinochloa and some interspecific hybrids) was very rapid in the 2,4-D free medium. Within a week, the young panicles of the first group distinctly sweelled then the buds began to develop from the spikelets after 15 days of inoculation. The frequency of DSP in this group was more than 70%. The response of the young panicles in the second group (O. perenis (512), O. officinalis (525) O. punctata (544), O. nivara (1050) and O. rufipogon Ï O. nivara (648) was not as rapid as that of the first group. A frequency of DSP around 20% was observed after 30 days of inoculation. The response of the young panicles in the third group) O. australiansis, Panicum maximum, Musa X paradisiaca (ABA) was rather tardy and slow, the frequency of DSP being less than 10%. The duration of DSP was more than one month. In the fourth group, the young panicles did not respond to the medium in this experiment. Besides the spikelet budding, sometimes adventitious bud could develop from the cutting place of the panicle axis usually the budding from the spikelets inhibited the growth of adventitious buds, but in this case the growth of adventitious bud could suppress the DSP. Some spikelets budded directly from the panicles of Setaria and Echinoshloa which were cultured in the same test tube, while the other spikelets of the same panicle were flowering. Calli were formed from some spikelets of Panicum and Oryza, while the others of the same panicle in the same test tube were budding.
Abstract (Browse 1922)  |  Full Text PDF       
Relation of Integrity of Outer Membrane with Its Protection from Oxygen in Anabaena Cell
Author: Wang Ye-qin, Feng Bo and Li Shang-hao
Journal of Integrative Plant Biology 1989 31(4)
      
    Exposure of Anabaena 7120 cells to membrane perturbant such as EDTA or Tris (pH 8.0, 37⊥, for 5每10 min) resulted in the release of outer membrane lipopolysaccharide and proteins from cells. After Tris treatment, the sensitivity of cells to crystal violet and detergents such as SDS and Triton x-100 increased and whole-cell alkaline phosphatase activity enhanced obviously, suggesting that the structure of outer membrane was modified and its permeability increased. At the same time. Tris was found to reduce nitrogen fixation activity of cells considerably in air, but not in anaerobic condition. Reconstitution of Tris-treated cells with released material might recover nitrogen-fixing activity of cells clearly, indicating that the structure of outer membrane is closely related to the protection of nitrogen fixation from oxygen. Although EDTA-treated cells released more lipopolysacharide and proteins than those of Tris-treated cells, the permeability of outer membrane and nitrogen-fixing activity were not influenced significantly. SDS-gel electrophoresis showed that Tris-treated cells released 3每4 specific polypeptides which were not present in the released material from EDTA-treated or water-treated cells. These experiments suggest that membrane perturbants-induced loss of outer membrane function is mediated through the modification of specific position in outer membrane.
Abstract (Browse 1782)  |  Full Text PDF       
Nitrogen Fixation and ATPase of Root Nodules from Sesbania cannabina with Chemical and Physical Treatments
Author: Jing Yu-xiang, Shan Xue-qin and Zhang Bao-tian
Journal of Integrative Plant Biology 1989 31(4)
      
    The nitrogenase activity of root nodules from Sesbania cannabina plants treated with Na2S2O4, DTT and trypsin was increased by 108%每114%, 106%每117% and 103%每119%, respectively. EM observation showed that the density of ATP-hydrolase as the marker of lead phosphate particles which were distributed on the peribacteroid membrane was much more significant than that of the control, but the bacteroids in peribacteroid membrane did not have ATP-hydrolase particles present. Dark treatment of the same age plants accelerated the nodule senescence and the ATP-hydrolase particles most densified on the peribacteroid membrane of the nodules, meanwhile, dense ATP-hydrolase particles also appeared in a number of degenerative bacteroids. This again confirms the conformation change of ATP-ase in bacteroids from ATP synthetase to ATP-hydrolase and its relation to nitrogen fixation with the senescence of nodules. The comparison of ATP-hydrolase particle density on the peribacteroid membrane of the nodule cells with different treatmemts are carried out and the role of the ATP-hydrolase on the peribacteroid membrane in substance transportation are discussed.
Abstract (Browse 1791)  |  Full Text PDF       
Cytological Studies on Triploid Lacquer Tree
Author: Shang Zong-yan, Li Ru-juan, Zhang Ji-zu and Liu Qian-hu
Journal of Integrative Plant Biology 1989 31(4)
      
    Rhus verniciflua Stokes is an economic tree endemic to China. In 1985, we discovered a natural triploid cultivar in the rich cultivar resources of Lacquer trees from Shaanxi province for the first time. The karyotypes of diploid and triploid lacquer trees are reported in this paper. They are K=2n=2x=30=14m+12sm+2st+2t; K=2n=3x=45=24m+15sm+3st+3t. 3t. By comparing the two karyotypes and studying chromosome pairing of the meiosis, it is proved that this triploid lacquer tree is a autotriploid.
Abstract (Browse 1969)  |  Full Text PDF       
Construction of Chloroplast Genomic Library of Amaranthus cruentus R104 and Cloning of the Gene for the Large Subunit of Ribulose-1, 5-disphosphate Carboxylase/Oxygenase
Author: Gan Lu, Li Xiao-bing, Hu Nai-bi, Yue Shao-xian and Zhu Li-huang
Journal of Integrative Plant Biology 1989 31(4)
      
    Grain amaranth is an annual food and forage crop and C4-plant with characteristics of rich nutrients, high photosynthesis efficiency and strong stress-resistance. Chloroplast DNA (ctDNA) was prepared from Amaranthus cruentus R104. The ctDNA was digested with restriction enzymes Barn Hi, EcoRi, Bgk, Psti and Saj, and the fragments thus obtained were electrophoresed in 0.7% agarose gel. The Length of the doublestranded ctDNA was measured to be 140 kb or so. BamHI-digested fragments of R104 ctDNA were inserted into pBR322 and a chloroplast genomic library has been constructed. The recombinant clone containing rbcL gene has been identified and selected out from the library. The restriction analysis of the clone showed that the rbcL gene of A. cruentus R104 has the structure similar to that of C4-plant corn. Moreover the reason of unsuccessful cloning of 5.9 kb BamHI fragment containing psbA gene is discussed.
Abstract (Browse 1909)  |  Full Text PDF       
New Steroidal Saponin of Stem and Leaf of Fritillaria ussuriensis Maxim
Author: Xu Dong-ming, Wang Shu-qin, Huang En-xi and Xu Mao-li
Journal of Integrative Plant Biology 1989 31(4)
      
    A new steroidal saponin, pingpeisaponin, was isolated from stem and leaf of Fritillaria ussuriensis Maxim by column chromatographic technique. On the basis of the IR, MS, 1H NMR and 13C NMR spectra of pingpei saponin, the structure has been established as 24汐-hydroxyl diosgenin-3-O-汐-L-rhomno-pyranosyl-(l-2)-汕-D-glucopyranoside.
Abstract (Browse 1949)  |  Full Text PDF       
Spiral Thickenings of Vessel Elements in Magnoliaceae in China
Author: Shu-ming and Li Zheng-li (Lee Cheng-lee)
Journal of Integrative Plant Biology 1989 31(4)
      
    Spiral thickenings in the inner walls of vessels were studied in 10 genera 69 species of Magnoliaceae in China under scanning electron microscopic observation. Spiral thickenings were found in some species. The majority of these species showed complete thickenings of the inner wall, however, in some of which the spiral thickenings were less obvious at both ends. In others, these spiral thickenings existed only in the middle portion, uniformly or irregularly distributed.
Abstract (Browse 2053)  |  Full Text PDF       
 

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