July 1989, Volume 31 Issue 7


          Research Articles
A Study of Shoot Apex Grafting is in Vitro for Virus Elimination Citrus
Author: Ma Feng-tong and T. Murashige
Journal of Integrative Plant Biology 1989 31(7)
Abstract (Browse 1874)  |  Full Text PDF       
A Salt Adaptation Protein in Cultured Carrot Cells
Author: Wang Hong-xin and Hu Zhi-ang
Journal of Integrative Plant Biology 1989 31(7)
    A protein accumulation has been observed in carrot cell line L104-2 after adapting to grow in Murashige and Skoog medium supplemented with 1% NaCl. The apparent molecular weight of its monomer was estimated at about 66 kD by using sodium dodecyl sulfate polyacrylamide gel electrophoresis. Gathered together with the fact that other osmotic reagents such as polyethyleneglycol did not induce its accumulation which indicated that this 66 kD protein is somewhat different from the 26 kD osmotins reported before from tobacco and tomato cells, therefore, it seems a salt adaptation protein.
Abstract (Browse 1869)  |  Full Text PDF       
The Morphology and Systematic Position of Uncatoella verticillata
Author: Geng Bao-yin
Journal of Integrative Plant Biology 1989 31(7)
    Uncatoella verticillata was first described by Li Xingxue anti Cai: Chongyang in 1978: from the Lower Devonian of Yunnan, China. It consists of slender internodes and swollen nodes with whorled branchlets. Li and Cai tentatively considered it as Incertae sedis in the' algae, because the reproductive organs were not found. The material described in this paper provided the new information on the reproductive organs (oogonia with vertical and segmented enveloping cells), which were collected from the original locality of Uncatoella in the Qujing region of Yunnan. On the basis of its gross morphology (vegetative and reproductive parts), Uncatoella can be assigned to Sycidiaceae of Sycidiales.
Abstract (Browse 2043)  |  Full Text PDF       
Studies on the Naphthaquinone Constituents of Onosma confertum W. W. Smith and Quantitative Determination of Shikonin
Author: Ai Ke-hui, Li Feng-ying, Li Yong, Wang Wei-bo and Wu Yu-yong
Journal of Integrative Plant Biology 1989 31(7)
    Four compounds were isolated from Onosma confertum W. W. Smith. They were identified as deoxyshikonine (), , -dimethylacrylshkonin (), acetylshikonine () and shikonin () according to their physical and chemical constants from MS, IR, 1H-NMR, CD-HPLC determination. The total quantity of shikonin obtained after basic hydrolysis of its derivatives was determined.
Abstract (Browse 2143)  |  Full Text PDF       
Studies on the Alkaloids of Stephania succifera Lo. et Tsoong
Author: Chen Yan, Fang Sheng-ding, Akinori Suzuki and Akira Isogai
Journal of Integrative Plant Biology 1989 31(7)
    Eleven alkaloids were isolated from roots of Stephania succifera Lo et Tsoong. They were identified as crebanine (), tetrahydropalmatine (), dehydrocrebanine (), palmatine (). dehydrocorydalmine (), crebanine nitrate (), oxocrebanine (), corydalmine (), phanostenine (), discretamine () and corypalmine () by means of TLC. UV, NMR MS spectral analyses and the fact that no depression of MP. with authentic samples was observed. Compounds , , , , , and were first reported from this plant.
Abstract (Browse 2124)  |  Full Text PDF       
Study on Chemical Components of Davidia involucrata Baill. Native to China
Author: Xiang Gui-qiong and Lu Fu-sun
Journal of Integrative Plant Biology 1989 31(7)
    Six compounds have been isolatea from the branch of Davidia involucrata Baill which is native to China. Their structures were identified as taraxerone (D1), taraxerol (D2), -sitoste- rol (D3), 3-O-methyl- 3, 4-O, O-methylideneellagic acid (D4), 3, 3, 4-5-trimethylellagic acid (D5) and ellagic acid (D6) by means of chemical methods and IR 1H-NMR, MS, respectively.
Abstract (Browse 2054)  |  Full Text PDF       
Structures of Sarcorucinine D and Pachyaximine A, B
Author: Qiu Ming-hua, Nie Rui-lin, Wang gun and Zhou Jun
Journal of Integrative Plant Biology 1989 31(7)
    In this paper, three new alkaloids, sarcorucinine D (1), and paehyaximine A, B (2, 3) were isolated from Sarcococca ruscifolia Stapf and Pachysandra axillaris Franch, respectively. The structures of sarcorucinine D (1) and pachyaximine A, B (2, 3) were elucidated to be 3-hydroxyl-20-dimethylamino-5-pregnane (1); and 3-methoxyl-20-dimethylamino-pregne-5-ne (2); and 3-methoxyl-16-hydroxyl-20-dimethylaminopregne-5-ne (16-hydroxyl-pachyaxi- mine A) (3), respectively.
Abstract (Browse 2076)  |  Full Text PDF       
Effect of Some Environmental Conditions on the Activity of the Nitrate Reductase-Stabi-lizing Factor (NRsf.)
Author: Gao Jin-hua and Li Zhi-zheng
Journal of Integrative Plant Biology 1989 31(7)
    The purpose of this work is to examine the relation between NR and NRSF in the leaves of the wheat plants under the different environmental conditions. The leaves of wheat plants which were grown in the nutritional solution containing NO3-, present 15 times higher of NR activity than that grown in the nutritional solution containing NH4+, but NRSF, which activated NO3-NR of the former increased only 20% as compared with that of the latter, indicating that NR is independent of NRSF. In addition, in the yellowing leaves dark grown in NO3-N solution and those in the same solution containing W with no nitrogen and Mo, the NRSF is quite stable, and keep higher activity, but no NR activity can be determined. So the NRSF is not considered as a major factor to regulate activity of NR in the leaves.
Abstract (Browse 1984)  |  Full Text PDF       
Preliminary Investigation of Glycine Aminotransferase in Spinach Leaf Peroxisomes
Author: Liang Zheng
Journal of Integrative Plant Biology 1989 31(7)
    Glycerate or OH-pyruvate was converted into serine by spinach leaf peroxisomes. The rate of conversion in intact peroxisomes was about 2.5 times higher than that in broken peroxisomes, with glycine as the NH2-donor. This higher converting rate in intact peroxisomes was not due to the increase of available OH-pyruvate concentration in intact peroxisomes, or the loss of cofactors to the apoenzyme in broken peroxisomes, also not due to the effect of active transferring system of glycine in peroxisomal membrane. The results suggested that there is a glycine aminotransferase in spinach leaf peroxisomes, and the high converting rate in intact peroxisomes was probably due to the difference of conformation or configuration of the glycine aminotransferase between the intact peroxisomes and broken peroxisomes.
Abstract (Browse 1866)  |  Full Text PDF       
Study on the Superoxide Dismutase of Soybean Seeds from Different Species in Subgenus
Author: Xu Bao, Zhuang Bing-chang, Lu Qin-hua and Lu Jing-liang
Journal of Integrative Plant Biology 1989 31(7)
    The SOD isoenzymes of different soybeans were studied in this experiment and the results showed that: 1. there were 7 SOD isoenzyme bands in all soybean genotypes used in this experiment, 2. the SOD activity (unit/g fr. wt.) in different soybean seeds decreased as the evolutionary process advanced, 3 the SOD activity decreased as the wt./100 seeds increased, and the color of seed coat changed from black to yellow. The relationship between SOD activity and the evolutionary process was discussed.
Abstract (Browse 1881)  |  Full Text PDF       
Ultrastructural Studies of Barley Mesophyll Cella lnfected with Barley Yellow Mosaic Viruses
Author: Lin Ya-kang and Yu Zhi-long
Journal of Integrative Plant Biology 1989 31(7)
    lexuous filamentous, rod-shaped particles, and laminated, pinwheel inclusions were observed in the mesophyll cells of the barley plants naturally infected with barley yellow mosaic viruses. These virus particles had a length of 480C920 nm and a width of 10C20 nm. In addition, bundles of filamentous structures which consisted of many particles with more 2000 nm in length were found in the leaves of the infected barley plants. The ultrastructural alterations of the infected mesophyll cells were rather conspicuous. The cytoplasmic matrix was lost seriously, and the chloroplast membrane system was destroyed. The cristae and matrix of the mitochondrium were decreased and some of them became vacuoles. The endoplasmic reticulum (ER) expanded teristic membranous network structures occurred in the cytoplasm of infected cells. The virus particles were often associated at one end with ER and with the membranes of network structures. The nucleus, membrane and wall of ceils also had somewhat variation.
Abstract (Browse 1998)  |  Full Text PDF       
Plate Culture of Cell Clone from Cathamus tinctorius
Author: Zhou Ping and Zheng Guang-zhi
Journal of Integrative Plant Biology 1989 31(7)
Abstract (Browse 1817)  |  Full Text PDF       
Study on the Establishment and Variation of Somaclones from Wheat-Wheatgrass Alien Additional Lines
Author: Wang Guan-lin, He Meng-yuan and Hao Shui
Journal of Integrative Plant Biology 1989 31(7)
    Calli were induced from the young leaves and mature embryos of seven wheat-wheatgrass alien additional lines, and somaclones were established from the calli. A large number of plantlets were obtained and survived after transplantation. The medium WG used for tissue culture of wheat-wheatgrass alien addition lines was set up in the experiment. The methods induced callus with two kinds of media were used. The first medium WG2 supplemented with 4 mg/l 2, 4-D and l mg/l NAA was used for callus formation. The second medium was WG2 with 2 mg/l 2, 4-D, 0.5 mg/l NAA and 0.25 mg/l KT. Differentiation medium used was WG3 with 0.5 mg/l KT, 1mg/l NAA and 100 mg/l adenylic acid. The examination of the chromosomes in regenerated plants indicated that the chromosomal number variation was remarkable. The plants maintaining 2n=44 were only 34.4% of the total regenerated plants, and the plants with the chromosomal number returned to 2n=42 made a relatively greater part of the variational plants regenerated. About half of the regenerated plants showed morphological variations. The crossover, translocation and other chromosomal structural changes were found in PMCs of the variational plants regenerated. Meanwhile, the polycentric chromosome derived from the fusion of several chromosomes and chiasmata of somatic chromosomes were clearly observed in callus cells, indicating that crossover and translocation occurred in somacloaes.
Abstract (Browse 1948)  |  Full Text PDF       
Studies on the Morphogenesis of the Xylem of Euonymus bungeanus Cultured in Vitro
Author: Zhang Xin-ying, Han Li-ling and Li Bai-ling
Journal of Integrative Plant Biology 1989 31(7)
    The isolated xylem of Euonymus bungeanus Maxim. has been successfully induced into plantlets for the first time under culture in vitro. The percentage of plantlet formation was about 8%. The process of cultivation was able to be devided into three stages: 1. To induce the formation of callus, for which the medium H showed the best effect, but it was necessary to supply with the different concentration of 2,4-D, NAA or BA. 2. The differentiation of the callus into plantlets, for which the medium H or MS+BA 2 mg/l+KT 0.5C1.0 mg/l+NAA 0.2 mg/l was most promising and 3. The growth of plantlets, for which the medium White+ NAA 0.1 mg/l+IAA 0.1/1 was most suitable. Further observations based on the histology showed that callus was produced from the ray cells. Meristematic tissue appeared as a discontinuous and more or less regularly arranged bands. Later, some of them were arranged in a cirle like in the normal stem. However, the newly formed meristematic tissue did not produce normal vascular tissues. They could only differentiate into tracheid-like elements on one side and no sieve-like elements differentiatedon the opposite side. In addition, there might be appeared nest-like tracheid mass in groups. The adventitious buds and roots derived from the excised xylem which followed the normal process of ontogenesis, i.e. the buds were from exogenous origin and the roots, endogenous origin.
Abstract (Browse 1953)  |  Full Text PDF       
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