August 1992, Volume 34 Issue 8


          Research Articles
The Sporo-Pollen Assemblage From Fuxian Formantion in Yulin-Hengshan Region, Shaanxi Province
Author: Yan Cun-feng
Journal of Integrative Plant Biology 1992 34(8)
    Eighty-eight species of spores and pollen referred to 66 genera including one new species from the Fuxian Formation in Yulin-Hengshan region of Shaanxi province have been studied. The sporo-pollen assemblage may be dividied into two subassemblages. The main characters are described as follows: There are 30.7% of spores of pteridophyta. The pollen grains of gymnospermae arno:rots for 69.3%. Among the spores, those of the genus of Deltoidospora (9.2%), Cyathidites (5.6%) and Undulatisporites (4.6%) are dominant, Among the pollen grains, those of the genus Classopoilis of Chiolepidiaceae is predominant (41.8%) and Chasmatosporites(6.1%) are comparatively abundant. Besides these, there are some other Jurassic common species found in the assemblage. Comparing with the known assemblages of other places, the author considers that the Fuxian Formation belongs to Early Jurassic Lias Series. The lower part of the formation might correspond the Hettangian stage or the Simemurian Stage It: the early Jurassic, the climate should be comparatively humid with flourishing vegetation in the early stage, and hot and arid in the middle-late stage.
Abstract (Browse 1752)  |  Full Text PDF       
Correlation between Protein Body Formation and Storage Protein Accumulation in Soybean Cotyledons
Author: Zheng Yi-zhi, He Meng-yuan and Hao Shui
Journal of Integrative Plant Biology 1992 34(8)
    At 20 days after flowering (DAF), the 7S 汐' and 汐 subunits began to accumulate. At 25 DAF, the 7S汕, l1SA and llSB subunits appeared. Five days later, the 11SA-4 subunit was present During the period of 25每55DAF, the storage protein content continued to increase. From 55 to 63 DAF, there was a decrease in the synthetic rate of the storage proteins. Comparing these results with the two paths of protein body formation reported previously, we draw the conclusion that the protein bodies developed from vacuoles contained not only the 7S bm also the lis proteins in soybean cotyledon cells.
Abstract (Browse 1742)  |  Full Text PDF       
Anther Wall of Cymbidium Goeringii with Special Reference to the Tapetum
Author: Qu Xin-shun,Li Rong-qian and Wang Jian-bo
Journal of Integrative Plant Biology 1992 34(8)
    The structure of the anther wall in Cymhidum goeringii (Rchb. f.)Rchb.f, was followed through two developmental stages using light and electron microscope: The peculiar anatomical pattern of the anther wall and the cytological characteristics of its tissues are described. Tapetum is the most notable feature in the anther wall. The specialized characteristics of the tapetal cells may be summerized as followings: (1) The tapetum may be distinguished into outer and inner tapetum, but they constitute the continuous layers surrounding the pollinium. The inner tapetum is derived from the connective tissue. (2) The outer and inner tapetum are composed of 2--3 cell layers. They have the same ultrastructural features and functions. (3) There are many lipid-containg plastids in the tapetal cells during their developmental stage. This is the main source of lipid bodies in the tapetal cells during their final developmental stage. The lipid-containing plasmids are derived from the extension of the nuclear membrane. (4) After the formation of tetrads, the secretion and the autolysates from of the tapetal cells not only deposition the surface of the pollinium to form the film, but also flow into the inner region of the polinia between the retrads contributing to their adhesion. The results of this study provide some new data of the tapetal structure and function in the angiosperms.
Abstract (Browse 1718)  |  Full Text PDF       
Anatomical Study of the Rachis of Compsopteris elliptica and the Systematic Position of Compsopteris
Author: Guo Ying-ting, Tian Bao-lin and Han De-xin
Journal of Integrative Plant Biology 1992 34(8)
    The anatomical characters of the rachis of Compsopteris elliptica ex Yang et Chen are described from the calc-petrified specimens of the Late Permian of Panxing, Guizhou, China. The main characters of its rachis are as follows (Plate I, 1每4; 6每9): The vascular bundles of 2每 2.5 cycles and ectophloic type. Phloem, consisting of 1每2 layers of cells. Protoxylem, composed of several small tracheids which are less than 20米m in diameter. Metaxylem, consisting of 2每3 layers of tracheids, about 30米m in diameter, scalariform thickenning. The sclerenchyma zone lies between the vascular bundles, its cell, small, 20每25 米m in diameter, some containing brown substances. The cortex could be divided into two zones: outer zone, consisting of thin-walled cells, and inner zone, thicken-walled cells, with secretory cavities. Epidermis, one layer, rectangular, some with contents. Compsopteris sp. (Plate I,5) 3每4 cycles vascular bundles, the same as C. elliptica in many respects; only larger than C. ellipptica in diameter. It may represent the base part of the rachis or larger rachis. Based on the shape of vascular bundles and the structure of xylem and phloem, Compsopteris is very similar to Angiopteris, Danaea etc., which all belong to the Marattiales. In addition, Huang et al. (1989) found that the sporangia of Compsopteris is similar to that of Danaeites, which belongs to the Maratriales. Therefore, Compsopteris doer not belong to the seed fern, bur the Marattiales.
Abstract (Browse 1871)  |  Full Text PDF       
Change of Sulfur Content in the Bark and Its Application in Monitoring the Air SO2 Pollution in Winter
Author: Liu Rong-kun,Jiang Fang and Tian De-lin
Journal of Integrative Plant Biology 1992 34(8)
    The sulfur content in different parts of the twig the same year is different, i.e. leaf bark wood. Variation exists in different tree species in seasons and appears to be in positive retation with atmospheric SO2 concentration. The bark of hibernal twig of the deciduous leaves possesses significant capacity of sulfur absorption which is interrelated with the total opening of lenticelle and is also influenced by the surface structure of stems as well. This paper presents a regression equation and a calculation chart for monitoring SO2 pollution by sulfur content detected in the bark in winter at some regions in Shenyang. The results are in concert with those determinated by the physical-chemical methods.
Abstract (Browse 1864)  |  Full Text PDF       
The Structures of Triptotetraolide and Wilforjine
Author: Deng Fu-xiao, Xia Zhi-lin, Xu Rong-qing and Chen Jun-yuan
Journal of Integrative Plant Biology 1992 34(8)
    A new diterpenoid lactone named triptotetraolide and a new sesquiterpene alkaloid named wilforjine were isolated from Tripterygium wilfordii Hook. f.. Their structures were elucidated on the basis of UV, IR, 1H-NMR, 13C-NMR, MS and X-ray analysis.
Abstract (Browse 1826)  |  Full Text PDF       
Isolation and Identification of Atractylenolide lV from Atractylodes macrocephala Koidz.
Author: Huang Bao-shan,Sun Jian-shu and Chen Zhong-liang
Journal of Integrative Plant Biology 1992 34(8)
    Nine compounds have been isolated from the rhizomes of Atractylodes macrocephala Koidz. One of them is a new compound, named atractylenolide l. Sitosterol and triterpenoid ester were also isolated firstly in this genus.
Abstract (Browse 1901)  |  Full Text PDF       
Studies on Cell Suspension Culture of Panax notoginseng
Author: Zhou Li-gang, Zheng Guang-zhi, Gan Fan-yuan, Wang Shi-lin and Xu Chun
Journal of Integrative Plant Biology 1992 34(8)
    The optimum period of harvesting in cell suspension culture of Panax notoginseng was 30 days. The time course of sap.nih formation proceeded almost in parallel with the cell growth. An appropriate concentration of oligosaccharms from Panax ginseng, precursor fames.l, mannffol and lysozymum which were added into tbe culture broth 10 days before harvesting, all induced saponin biosynthesis significantly. Oligosaecharins at a concentration of 15ppm(it increased 1 fold of saponin yield, and increased 22.7%(of cell growth rate compared with those of the control) and farnesol at 200ppm(it increased 70.5% of sap.nih yield and stimulated cell growth compared with those of the control) were more effective.
Abstract (Browse 1777)  |  Full Text PDF       
Study on Hairy-Root Cultures of Fagopyrum cymosum
Author: Zhang Yin-lin, Lu Gui-lan, Zhou Xin-hua, Zhao Bao-hua and Li Ying
Journal of Integrative Plant Biology 1992 34(8)
    Transformed hairy root cultures of Fagopyrum cymosum (Trey.) Meisn. have been established by infecting petioles of shoots cultured in vitro with Agrobacterium rhizogenes. These hairy root cultures grew vigorously in MS hormone-free medium. They showed a 1861-fold increase in fresh weight with in 25 days, and grew faster than cell cultures of F. cymosum (only increased 26.7-fold with in the same days). The cultures were shon to synthesis dimeric procyanidin up to 4.5% (dry wt.) approximating the level of original plant. The growth rates of hairy root cultures up to 149.3 mg dry w-t/L/day in large culture vessels (3 L) and dimeric procyanidin (3.58%, dry wt.) were detected.
Abstract (Browse 1834)  |  Full Text PDF       
Effects of Mineral Nutrition on Water Status and Osmotic Adjustment in Spring wheat under Different Moisture Conditions
Author: Xu Meng and Shan Lun
Journal of Integrative Plant Biology 1992 34(8)
    The 朵w and RWC of the leaves of spring wheat at low level of nutrition were higher than those of the spring wheat leaves at high level during the slow drought period. Therefore, the plants at high nutrition level were more sensitive to drought. High mineral nutrition did not enhance the maximum osmotic adjustment but changed the regulatory process of osmotic adjustment under drought condition. During moderate to nearly severe drought period higher osmotic adjustment was shown in the leaves of spring wheat at high nutrition level. The major solute contributed to osmotic adjustment in the leaves of the spring wheat was soluble sugar, mostly reducing sugar.
Abstract (Browse 1737)  |  Full Text PDF       
Isolation and Characterization of Photosystem j Reaction Center D1-D2-Cytochrome b-559 Complex from the Chloroplasts of Spinach
Author: Yu Zhen-bao, Kuang Ting-yun, Tang Chong-qin, Peng De-chuanㄛZheo Qi, Tang Pei-song and Li Cheng-yong
Journal of Integrative Plant Biology 1992 34(8)
    Photosystem j reaction center D1-D2-cytochrome b-559 pigment-protein complex has been isolated and purified from chloroplasts of spinach and its properties have been studied. The Isotared photosystem II reaction center contains close to six chlorophyll a per two pheophytin a molecules. Analysis of fluorescence decaying by phase modulation fluorometry suggests that the reaction center has three components of fluorescence decaying with lifetimes of 1.5 nS, 6.23 nS, 36.26 nS in terms of fractions to total fluorescence yield as 0.06, 0.67, 0.27 respectively. The ,6.25 nS fluorescence component corresponds to chlorophyll a which is energetically uncoupled from the process of charge separation. The proportion of 1.51 nS component is very low, and its source is unclear. The 36.25 nS fluorescence component is attributed to the recombination of the primary radical pair, and so represents the activity of charge separation.
Abstract (Browse 1746)  |  Full Text PDF       
Nutrient Source of Sclerotia of Grifola umbellata and Its Relationship to Armillaria mellea
Author: Guo Shun-xing and Xu Jin-tang
Journal of Integrative Plant Biology 1992 34(8)
    Ways nutrient-uptake of sclerotia of Grifola umbellata and the relationship between G. umbellata and Armillaria mellea were studied. At the primary stage of sclerotia of G. umbellate infected by A. mellea, the hyphae of G. umbellata could obtain nutrients by invading the one- to three-layer-cells of A. mellea cortex which existed in the sclerotia of G. umbellata, ar late stage of A. mellea infection, the nutrient source of sclerotia of G. umbellata mainly depended on its hyphae, adhering on the intercellular space of A. mellea, to suck the metabolic products of A. mellea. After being nourished the hyphae of sclerotia of G. umbellata outside of the rhizomorph of A. mellea began to reproduce, as their nuclear divisions were well observed. The results suggested that the mutual assimilation between G. umbellata and A. meIlea could be defined as a form of special Symbiotic relation.
Abstract (Browse 2172)  |  Full Text PDF       
Relationship between the Nucleo-Cytoplasm Composition and the Kinetic Properties of Ribulose-l,5-bisphosphate Carboxylase/Oxygenase of Rice
Author: Ning Zheng-xiang, Wan Bang-hui and Li Ming-qi
Journal of Integrative Plant Biology 1992 34(8)
    Ribulose-l,5-bisphosphate carboxylase/oxygenase (RubisCO, EC was purified from leaves of rice (Oryza sativa), including four fertile cultivars and six male-sterile nuclear substitution lines: genome of cultivars in O. sativa L. f. spontanea cytoplasm and in O. sativa subsp. indica cv. Gambiaka Kokum cytoplasm, respectively. These RubisCO enzymes were divided into, two categories: (a) RubisCO with identical large subunits but different small subuinits, (b) RubisCO with variable large subunits and identical small subunits. Specific activities for both the carboxylation and oxygenation reactions of RubisCO were determined under standard conditions of activation. It was found that the Vmax(CO2) remained constant, but Km(O2) changed greatly, ranging from 223 米mol/L to 371 米mol/L in (a) group. The small subunits had significant effect on Km(O2) and on Vmax(O2)/Km(O2). No significant variation in Km(CO2), Vmax(O2), and the specificky factor were detected among the RubisCO with varied small subunits. Significant variation in Km(O2) and specificity factor were detected among the enzymes with varied large subunits, which also showed an important effect on Km(CO2) and Vmax(O2). RubisCO with heterologous large subunits had higher carboxylase activity and specificity factor than those with homologous large subunits.
Abstract (Browse 1954)  |  Full Text PDF       
The Puff-Like Structure in Allium cepa and the Effect of 汐-Amanitin Treatment upon the Structure
Author: Xing Miao, Jiao Ming-da, Tong De-juan and Hao Shui
Journal of Integrative Plant Biology 1992 34(8)
    The puff-like structure (PLS) is a loose, fibrillar, spherical structure which has been found in the nucleus of some higher plants and often referred to intranuclear bodies, centromeres, micropuffs and puff-like fibrillar structures. Opinions on the nature of the PLS are still diverged. In order to gain further information upon the nature of the structure, ultrastructural and cytochemical features of the PLS in the nucleus of root tip meristematic cells of Allium cepa L. and the effect of 汐-amanitin(汐-A, a specific inhibitor of RNA polymerase j which is responsible for the transcription of genes coding for mRNA) on it were studied. Two types of PLSs were found in the conventionally-stained samples: one was composed of compact fibres while the other consisted of loose, decondensed fibres. In the PLS of the latter type, a brush-like structure comprising both an axial fibre and lateral fibres was observed, suggesting that transcription was taking place in the PLS. When stained by Bernhard's method, the PLS was shown to contain both RNP and DNP. After the treatment with 汐-A, the PLS composed of compact fibres was still seen but the one consisting of loose fibres was destroyed ultrastructurally and the brush-like structures disappeared. Based on these observations, it is suggested that the PLS would be a morphological manifestation of transcription of the non-nucleolar gene coding for mRNA.
Abstract (Browse 1915)  |  Full Text PDF       


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