April 1993, Volume 35 Issue 4


          Research Articles
The Structure of a New Monoterpene Glycoside From Aster Tataricus L.F.
Author: Cheng Dong-liang,Shao Yu,Yang Li and Zou Pei-xiu
Journal of Integrative Plant Biology 1993 35(4)
    A new monoterpene glycoside named shio nc, side C was isolated from the root of Aster tataricus L. f. (Compositae). lts structure was elucidaied as L-endo-camphanol-8-O-3,5-isopropylidene-¦Â-D-apiofuranosyl- (1-6) -¦Â-glucopyranoside ( ¢ñ ) by means of spectral and chemical evidence.
Abstract (Browse 1814)  |  Full Text PDF       
The Secoiridoides Isolated from Swertia nervosa(G. Don)Wall. ex C. B. Clarke
Author: Luo Yue-hua and Nie Rui-lin
Journal of Integrative Plant Biology 1993 35(4)
    Four secoiridoid glycosides were isolated from Swertia nervosa (G. Don) Wall. ex C.B. Clarke. Three of them were identified as vegeloside (¢ñ), sweroside (¢ó) and swertiamarin (¢ô)on the basis of spectral and chemical evidences. Another compound, nervoside (¢ò), was a new secoiridoid glycoside, its structure was elucidated by means of UV, IR, 1H and 13C-NMR spectra. Nervoside was a 7-¦Á-OCH3 isomer of vegeloside, this was confirmed by NOE experiment.
Abstract (Browse 1892)  |  Full Text PDF       
Studies on the Constituents of the Flavonoids from the Roots of Sophora flavescens Ait
Author: Zhao Yu-ying,Wang Bin,Lei Li-ming,Guo Min-jie, Zhang Ru-yi,HuangLi-ru Yi You-yun and Lou Zhi cen
Journal of Integrative Plant Biology 1993 35(4)
    Eight flavonoids were isolated from the roots of Sophora flavescens Ait. On the basis of their physical and chemical properties and spectral analysis, they were identified as maackiain (1), 4-methoxy-maackiain (2), trifolirhizin (3), nor-anhydroicarithin(4), isokuratione (5), sophoraflavanone B (6), nor-kurarinone (7)and formoronetin (8). Com-pounds 1,2 and 6 were found in Sophora flavescens Ait for the first time.
Abstract (Browse 1809)  |  Full Text PDF       
The Effect of the Induced Substances from Collectotrichum langenarium Related to some Enzyme Activities of Disease Resistance in Xin-jiang Melon
Author: Li Guan
Journal of Integrative Plant Biology 1993 35(4)
    Xingjiang melon (Cucumis melo L.) was treated respectively with elicitors from culture filtrate (El) and mycelial walls (Ew) of Colletotrichum langenarium. Activities of phenylalanine arnmonialyase (PAL) and peroxidase (POD) were significantly higher in the treated pNnts. The effect of inducement was higher with El than with Ew. A new protein from plants treated with El was found on 5-20% polyacrylamide gel gradient electrophoresis. The Rf value of the new protein was 0.7-0.75. But no new protein was found in plants treated with Ew.
Abstract (Browse 1703)  |  Full Text PDF       
Climatic Gradiant of Main Vegetation Types in the Loess Plateau Region
Author: Wang Yi-feng and Xiao Xiang-ming
Journal of Integrative Plant Biology 1993 35(4)
    Based on the climatic data from 286 meteorological stations on the loess plateau China, Thornthwaite¡¯s method (1948) was used to calculate the annual adjusted potential evapotranspiration (APE), moisture index (IM), thermal efficiency index (TE), aridity index (IA) and humidity index (IH). Geographical regression models for each index were developed and applied to generate regional distribution maps for each index. For instance, APE=1812-8.79 Lat-5.91 Long-0.17Alt. APE and IM were closely related to main vegetation types and their distribution on the Loess Plateau. Most vegetation types had negative IM and were usually classified into the dry subhumid-semiarid-arid region. TE of most vegetation types was within the range of 57.1-85.5, belonging to mesothermal category. Zonal vegetation on the southern Loess Plateau was xeric forest, woodland and shrubland, which are different from Quercus liaotungensis kolz. forest on mountains in terms of community characters and distribution.
Abstract (Browse 2074)  |  Full Text PDF       
Kinetic Analysis of the Effect of Lanthanum Chloride on Potassium ion Exudation of Corn Root Segments
Author: Jiao Gen-lin,Tang Xi-ke and Wu Zhao ming
Journal of Integrative Plant Biology 1993 35(4)
    The effects of LaCl3 on membrane permeability and the secretion of proton of the root segments of corn (Zea mays L.) ¡°Jing Zhao No. 8¡± were studied, and the process of K+ exudation was analyzed by means of kinetic method. The exudate conductivity and leakage of K+ and sugar were lowered, and proton secretion was enhanced by LAC18 treatments in corn root segments. Kinetic analysis of K+ exudation illustrated, that (1) mathematical model used for absorption study was suitable for study of exudation process, (2) the maximum absorption rate (Vmax) was increased and Michaelis constant was unchanged in presence of LaCl3 and/or CaCl2, (3) the lowering of K+ leakage was due to the increased Vmax in presence of LaCl3 and/or CaCl2.
Abstract (Browse 1828)  |  Full Text PDF       
Cytological Observations on Nodules,Nodule-Like Structures and Crown Galls on the Roots of Phaseolus vulgaris
Author: Yang Ye hua,Zhou Jun-chu,Zhang Zhong-ming and Shen Hui
Journal of Integrative Plant Biology 1993 35(4)
    The transconjugant of Agrobacterium tumefaciens with C58 genetic background containing plasmid pSym3622 of Rhizobium leguminosarum by. phaseoli was capable of infecting the roots of Phaseolus vulgaris of the wounding sites and inducing the formation of nodule-like structures near the sites of inoculation. These structures were different from the nodule and crown galls induced respectively by R. leguminosarumbv, phaseoli 3622--15 and a virulant strain A208 (pTiT37) of A. tumefaciens. The vascular bundles of the nodule-like structures were located at the central region of the structures and were surrounded by highly vacuolated thin-walled cells rich in starch grains. There was no bacterium neither in cell nor in the intercellular space of these structures evidenced by light and electron microscopic observations. In contrast, many metabolically active bacteroids were observed within the cells and in the intercellular spaces of the effective nodules, in the case of crown galls, there was also no bacterium in any part of the tumours. The internal structures of tumour on the root of Phaseolus vuigaris were visualized as distinguishable coherent areas of cells partitioned by disorganized cell zones. These areas of cells were traced back to be derived from different meristematic zones. At some instance, a number of root-like protrusions which were likely to develop into new roots were observed. In other parts, host cells were visible as a result of cell degeneration.
Abstract (Browse 1948)  |  Full Text PDF       
Comparative Anatomical Studies on the Wood Rays of Sixty-Six Species in Nine Genera of Magnoliaceae in China
Author: Wu Shu-ming,Lin Jin-xing and Li Zheng-li(Lee Chenglee)
Journal of Integrative Plant Biology 1993 35(4)
    A systematical study of the types, height and width of wood rays of sixty-six species in nine genera in Magnoliaceae an China was carried out. The wood rays of major genera and species in the family are heterogeneous rays of type IlA and type IIB. The uniseriate rays are all low rays, the width of which is less than 0.05 mm and belongs to fine group. The multiseriate rays are medium and low rays, the width of which ranges from fine, medium to slightly wide. These characters indicate that Magnoliaceae is a quite primitive family, but it is more specialized than Tetracentron sinensis Oliv., Trochodendron analioides Sieb. et Zucc. and Winteraceae, which also have heterogeneous type I. In addition, these imply that subfam. Magno-lioideae is even more primitive than subfam. Liriodendroideae, and tribus Magnolieae is much primitive than tribus Nichelieae in subfam. Magnolioideae. However, the differences of wood rays are less distinctive in taxa of genera and below genera. Therefore, it is difficult to determine their systematic position merely according to the structure of wood rays.
Abstract (Browse 1866)  |  Full Text PDF       
Embryological Studies in Rheum palmatum L.
Author: Chen Chao and Wang Yao-zhi
Journal of Integrative Plant Biology 1993 35(4)
    Embryology of Rheum palmatum L. has been studied. The main results are as follows. 1. The anther is four sporangiate with monoor multicellular archesporium. The development of anther walls conforms to the monocotyledonous type. The cells of the glandular tapetum become two nuclei in the later stage. The microsporocyte undergoes simultaneous meiotic division and produces tetrahedral tetrad. The pollen grains with three furrows are shed at the 3-celled stage. 2. The ovary is unilocular and contains only one ovule, bitegmic and crassinucellate. The micropyle is formed by the inner integument. The develoment of embryo sac conforms to the Polygonurn type. The single hypodermal cell in nucellus functions as archesporium. The megaspore mother cell undergoes meiosis to form a linear or T-shaped tetrad. Only the chalazal megaspore functions and forms the female gametophyte, whereas the other three degenerate. Two polar nuclei fuse before fertilization takes place. Three antipodal cells persist up to globular embryo stage. 3. The endosperm is of nuclear type. Endosperm nuclei form cells since the later stage of the globular embryo. However the chalazal endosperm nuclei do not form cells. They form a free nuclei endosperm haustorium. The phenomenon of free nuclei fusion has found in the haustorium and other part of the embryo sac. The development of embryo belongs to the Asterad type and the embryo has a small suspensor. 4. The hypostase appears at the stage of the mature embryo sac. It persists for a long time and only leaves a trace at the organized embryo stage.
Abstract (Browse 1935)  |  Full Text PDF       
Cytoplasmic Ultrastructural Changes During Microsporogenesis of Gossypium hirsutum:With Emphasis on
Author: Wang Yi and Hu Shi-yi
Journal of Integrative Plant Biology 1993 35(4)
    Conspicuous cytoplasmic changes took place during the microsporogenesis of Gossypium hirsutum L. These changes mainly involved in the ribosomes, plastids and mitochondria. During meiotic prophase 1, the ribosome population of the cytoplasm diminished and reached to aminimum during pachytene--diplotene interval, and the membrane structures of both plastids and mitochondria turned unclear. In metaphase I, cytoplasmic ribosome population restored to premeiotic level; plastids and mitochondria also regained their normal structures. The disintegration of nucleoloids from nucleus was the main mechanism for the restoration of ribosome population in metaphase I cytoplasm. Endoplasmic reticulum may play an important role in the elimination and protection of part of cytoplasmic ribosomes during prophase I. These obvious cytoplasmic changes are considered to be relevant to sporophyte-gametophyte transition.
Abstract (Browse 1730)  |  Full Text PDF       
A Membrane-Bound NPA-Binding Site in Acer pseudoplatanus L. Suspension Culture Cells
Author: Zhang De-yi,Xing Ti,Chen Rui,Wang Huai-jun and Ding Ru-xian
Journal of Integrative Plant Biology 1993 35(4)
    A specific NPA N-l-naphthylpathalamic acid) -binding site with high affinity was found in the membrane component of Acer pseudoplatanus L. suspension culture cells. The dissociation constant (Kd) of NPA was approximately 7.5¡Á 10-9mol/L. NPA binding was maximal after 60 min of incubation at 4¡æ. Synthetic auxin I-NAA, 2-NAA, 2,4-D and auxin transport inhibitor 2,3,5-TIBA were all able to compete with NPA for the binding. Site with Kd values ranging from 10-6 to 10-4 mol/L. These binding sites could be involved in the regulation of intracellular IAA level, thereby affecting cell growth.
Abstract (Browse 1848)  |  Full Text PDF       
Genetic Engineering of Plant Hormones
Author: Ma Qing hu and Song Yan-ru
Journal of Integrative Plant Biology 1993 35(4)
    The genetic engineering of plant hormones is a new developing research field in plant molecular biology. It will not only have an enormous impact on the research of the plant hormones, but also bear a potential much benefit f or the agricultural production.This article gives a comprehesive review on the above mentioned aspects, and its update advancement will also be discussed.
Abstract (Browse 1796)  |  Full Text PDF       
A Cytological Study of the Genus Ammopiptanthus
Author: Pan Bo-rong and Huang Shao fu
Journal of Integrative Plant Biology 1993 35(4)
    The present paper deals with a karyotype of two species of the genus Ammopiptanthus. The chromosome numbers in root-tip cells of these two species are both 2n=18. The karyotype formulas of the two species are as follows: A. mongolicus K(2n)=18=10m+4m(SAT)+2sm+2st; A. nanus K(2n)=18=8m+2m(SAT) +2sm+2sm(SAT) +4sm(SC). Results obtained through chromosome examination have shown that they both belong to "2A" type of Stebbins¡¯karyotypic asymmetry. But there are differences in chromosome formulas, it is assumed that A. nanus karyotype is a little more advanced than of A. mongolicus.
Abstract (Browse 1784)  |  Full Text PDF       


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