July 1994, Volume 36 Issue 7


          Research Articles
Plagionicin A, With C5-OH, A New Monotetrahydrofuran Acetogenin
Author: Zheng Xiang-ci, Yang Ren-zhou, Xu Ren-sheng and Qin Guo-wei
Journal of Integrative Plant Biology 1994 36(7)
    Compounds P4 and P5 had been isolated from the seeds of Polyalthia plagioneura Diels. The former was identified as howiicin A by the spectral analysis of [ ¦Á ]D, IR, 1 H - NMR, 13C-NMR and MS, the latter was characterized as a new monotetrahydrofuran annonaceous acetogenin with C5-OH, which was named plagionicin A.
Abstract (Browse 1712)  |  Full Text PDF       
Discovery and Significance of the Genus Cardiopteris
Author: Chen Fen, Sun Ke-qin and Zhou Hong-rui
Journal of Integrative Plant Biology 1994 36(7)
    Cardiopteris ningxiaensis sp. nov. was described from the Carboniferous Namurian strata in Zhongning County of Ningxia, China, and the geographical distribution, geological age and evolutionary significance of the genus Cardiopteris was discussed.
Abstract (Browse 1716)  |  Full Text PDF       
DCA Ordination, Quantitative Classification and Environmental Interpretation of Plant Communities in Dongling Mountain
Author: Jiang Hong, Huang Jian-hui, Chen Ling-zhi, Yang Chao-yang and Yang Xiao-qiu
Journal of Integrative Plant Biology 1994 36(7)
    Basing on the detrended corespendenee analysis (DCA) , twb-way indicative species analysis (TWINSPAN) and environmental interpretation mathematical models and warm temperate deciduous-broad leaf forest community samples collected from Dongling mountain in Beijing, primary vegetation types and ecological gradients, and their quantitative relations with environmental factors of the region were studied. The data from 26 climate stations in this region were used to get the multivarient regression for estimating the climate information of various warm temperate deciduous-broad leaf forest according to longitude, latitude and altitude of each sample plot. It is shown that the vegetation types of warm temperate deciduous-broad leaf forest and their distribution are mainly determined by the thermal and moisture ( include soil fertility) gradients. In moisture gradients, in mid-montane from drier to moisture there are Spiraea trilobata shrubland-Vitex negundo shrubland-Prunus sibirica shrubland -manmade Pinus tabulaeformis forest-Quercus liaotungensis forest -mixed deciduous-broad leaf forest- Betula dahurica forest; In subalpine from drier to moisture there are meadow-Caragana jubata shrubland-Betula costata forest. In thermal gradients, from warm to cold there are manmade Pinus tabulaeformis forest-Virex negundo shrubland¨CQuercus liaotungensis forest -Betula dahurica forest-mixed deciduous-broad leaf forest -Spiraea trilobata shrubland -Prunus siberica shrubland -manmade Larix princips forest-Betula platyphylla forest - B. costata forest-subalpine meadow -Caragana jubata shrubiand . Vegetation of Dongling mountain exists 4 polars anal 2 centers, 4 polars are subalpine Caragana jubata shrubland ( drier and cold habitat), mid-low montane Virex negundo shrubland (drier and warm habitat), subalpine B. costata forest (moisture and cold habitat) and mid-montane B. dahurica forest (moisture and warm). Two centers are Quercus liaotungensis forest and mixed deciduous-broad leaf forest (actuality) , and subalpine coniferous forest (the spruce and larch forest, is the potential center). The dominant and indicative plant species are Aconitum sinomontanurn (cold and moisture), Poa botryoides , Dendrantherna zavadskii, Aconiturn kusnezoffii and Carex rigescencs (cold and drier), Maianthemurn bi foliurn and Corylus rnandshurica (warm and moisture) Spiraea trilobata and Prunus siberica (warm and drier). And these species could be indication species for succession, its would indicate the state of pioneer communities. The Plant-geographic mathematical model of DCA axles (1 and 2) and environment factors (climate and soil) have been set up.
Abstract (Browse 2326)  |  Full Text PDF       
Stable Carbon Isotope Ratio and Activities of PEP Carboxylase and PEP Carboxykinase in Pineapple Leaves
Author: Lin Zhi-fang, Peng Chang-lian, Lin Gui-zhu and Li Shuang-shun
Journal of Integrative Plant Biology 1994 36(7)
    Slight flutuation in carbon isotope values were found in counted from top dounward to the 35th in pineapple, Ananas comosus (L.) Merr., but more negative ¦Ä13C value (less heavier 13C) was observed in lower position leaves. The average ¦Ä 13C value was ¨C12.94¡ë in 11 leaves with maximum range of variation as ¨C2.06¡ë. Similar single peak curves were found between PEPCase and PEP carboxykinase activities with leaves at various positions. Both enzymes reached the maximum activity in 8¡ª11th leaves, then declined in others at lower positions. PEP carboxykinase activity was 3.4 folds higher than PEPCase activity under the present experimental condition (25¡ª30 ¡æ). The results indicated that metabolic coordination evisted between dark carboxylation and light decarboxylation. For the obligate CAM plant, pineapple, though the carboxylation and decarxylation activities did occur in old leaves, the CAM level change did much, however.
Abstract (Browse 1805)  |  Full Text PDF       
Isolation and Characterization of Total DNA from Several Endangered Species and Their Allies
Author: Zou Yu-ping,Wang Xiao-quan, Lei Yi-ding, Pei Yan-long and Zhang Zhi-xian
Journal of Integrative Plant Biology 1994 36(7)
    A low pH extraction medium with high salts, which avoids ionization and subsequent oxidation of phenolic compounds during tissue grinding and precipitation of large amounts of materials, were successfully used to obtain total DNA from Cathaya argyrophylla Chun et Kuang, Paeonia suffruticosa var. spontanea, Cimici fuga nanchuanensis Hsiao, Adenophora potaninii (Congeneric species with A. lobophylla) etc. The DNA yields, quality and purity were characterized. These isolated DNA could be used directly for RFLP and RAPD analysis which are useful as molecular genetic markers without sedimentation in cesium chloride gradient or column chromatography. A fast, inexpensive and reliable procedure has been developed for detecting the genetic diversity of endangered plants.
Abstract (Browse 1757)  |  Full Text PDF       
Effects of Polyamines and Kinetin on In Vitro Frond Senescence in Lemna aequinoctialis 6746
Author: Yin Lu-ming
Journal of Integrative Plant Biology 1994 36(7)
    Both polyamines and kinetin could retard the loss of chlorophyll during dark-induced senescence in excised frond of Lernna aequinoctialis 6746. The effect of polyamines on retarding the chlorophyll loss was stronger than that of kinetin. Kinetin remarkably inhibited the loss of soluble proteins and the increase of protease activity, while no similar effects were observed from polyamines. An inhibitor of polyamine biosynthesis, methylglyoxal bis- (guanyl- hydrazone) (MGBG), slightly increased the loss of chlorophyll and soluble proteins. During senescience, both the increase of putrescine (Put) content and the decrease of spermidine (Spd) content were inhibited by kinetin at the concentration of 0.05 mmol/L, but the spermine (Spm) level was not affected by kinetin. The arginine decarboxylase (ADC) activity was dominant in frond of Lemna aequinoctialis 6746. Kinetin slightly increased ADC activity, while it had no marked effect on ornithine decarboxylase (ODC) and s-adenosylmethionine decarboxylase (SAMDC). The possible relationship between polyamines and cytokinins in retarding senescence was also discussed.
Abstract (Browse 1866)  |  Full Text PDF       
The Electrical Analogy Analysis and Simulation Testing of the Relationship Between Net Photosynthetic Rate and Stomatal Conductance
Author: Fu Wei and Wang Tian-duo
Journal of Integrative Plant Biology 1994 36(7)
    The hypothesis about the linear relationship between net photosynthetic rate and stomatal conductance, which had formed the basis of the empirical model proposed by Ball et al, was analyzed by analogy with electrical resistance and simulation testing. The results showed that the relationship between stomatal conductance and net photosynthetic rate was non-linear, but approaches a linear one when there was a large the boundary layer conductance of leaf. In addition, the way in which the ratio of CO2 concentrations in and out of the stomata changes with light and the influence of boundary layer conductance on the ratio had also been simulated.
Abstract (Browse 1716)  |  Full Text PDF       
The Relationship Between the Metal Clusters and the Conformation of Molybdenum-Iron Protein from Azotobacter vinelandii
Author: Huang Ju-fu, Luo Ai-ling, Hu Chang-zheng, Zhong Ze-pu, Wang Zhi-ping, Lin Yong-qi, Dong Qing-chu, Cheng Yu-hua and Li Jia-ge
Journal of Integrative Plant Biology 1994 36(7)
    The ultraviolet CD spectrum of nitrogenase MoFe protein from Azotobacter vinelandii had a negative trough with double peaks at 208 nm and 222 nm, respectively, and the shape of the trough was similar to those of other proteins with a-helix structure. After treatment with o-phenanthroline under an aerobic or anaerobic condition, the height of the peak at 222 nm (h222 nm) decreased with the decrease of the C2H2-reduction activity, Fe content and CD spectra at both 450 nm and 660 nm, or at 450 nm of the treated proteins. However, after reconstituting with a reconstituent solution containing Na2MoO4, Na2S, dithiothreitol and either ferric homocitrate or ferric citrate, the h222 nm Of the reconstituted proteins could be restored as well as the activity, Fe content and CD spectra at both of 450 nm and 660 nm. The results show that there is a significant relationship between the metal clusters (FeMoco and P-cluster) and the conformation of MoFe protein.
Abstract (Browse 1710)  |  Full Text PDF       
The Keratin-Like Intermediate Filament Network Exists in the Chlamydomonas sp.Pyrenoid
Author: Fan Bin, Li Yin-zhen and Zhai Zhong-he
Journal of Integrative Plant Biology 1994 36(7)
    The 10 nm diameter filement network in the Chlamydomonas pyrenoid was investigated using selective extraction and diethylene glycol distearate embedding-free electron microscopy. The pyrenoid was cross reacted with animal keratin-antibody using electron microscopic immunogold labeling. Indirect immunofluorescence observation showed the same result. These suggest that a keratin-like intermediate filament network does exist in the Chlam ydomonas pyrenoid.
Abstract (Browse 1872)  |  Full Text PDF       
Steroidal Glycosides from Aspidistra leshanensis K. Y. Lang et Z. Y. Zhu
Author: Chen Meng-jing
Journal of Integrative Plant Biology 1994 36(7)
    Two steroidal glycosides were isolated from the rhizome of Aspidistra leshanensis K. Y. Lang et Z. Y. Zhu. Their structures were established as 5-O-¦Â-D-glucopyranosyl-convalla- genin-B and aspidistrin on the basis of chemical and spectral evidences. Diosgenin, isonarthogenin, convallagenin-B, ¦Â-sitosterol and a sapogenin with three hydroxyls were obtained from the acid-treated n-BuOH extract.
Abstract (Browse 1833)  |  Full Text PDF       
Comparative Anatomical Observations of Wood Structures of Pinus yunnanensis and P.yunnanensis var. pygmaea
Author: Li Zheng-li(Lee Cheng lee), Fan Yong-jun and Cui Ke-ming
Journal of Integrative Plant Biology 1994 36(7)
    Pinus yunnanensis Franch. and P. yunnanensis Franch. var. pygmaea (Hs¨¹eh) Hs¨¹eh were cultivated at the same area and in the same year. It was found that P. yunnanensis var. pygmaea maintains its dwarfism even growing in a suitable habitat. The wood structures of this dwarf pine exhibit several characters similar to those of the phenotypic dwarf pine, such as that the length of tracheids is shortened; the diameter becomes narrowed; resin canals increase and more or less concentrate in the late wood; the boundary between early wood and late wood is indistinct, etc. However, it is rather difficult to find the twisted tracheids which is commonly seen in the phenotypic dwarf pine.
Abstract (Browse 2074)  |  Full Text PDF       
Laticifer Ultrastructural and Immunocytochemical Studies of Papain in Carica papaya
Author: Zeng Ying, Ji Ben-ren and Yu Bing
Journal of Integrative Plant Biology 1994 36(7)
    Cotyledons of germinating papaya (Carica papaya L. ) seeds and exocarp of young fruits were used as materials for study. The ultrastructural changes occurring during differentiation of laticifer and the ultrastructural environment of papain synthesis were studied by means of TEM and immunocytochemistry. Electron microscopic observations showed that the differentiating laticiferous cells were rich in ribosomes and mitochondria. Endoplasmic reticulum (ER) was well developed and apparently active, forming secretory vesicles of various sizes. With further development, organelles were gradually degenerated and autophagy of cytoplasm within vacuole was evident. ER was dilated and split into fragments. Cell wall perforations occurred at several sites of adjacent laticifer elements. Towards maturity, laticifer was fully filled with vesicles containing electron-dense materials. Organelles disappeared thoroughly but plasmalemma remained. Sections were incubated with anti-chymopapain antibodies followed by goat-anti-rabbit IgG-gold. Labeled gold was found predominantly in ER and the associated vesicles of differentiating laticifer. Several controls were used to establish the specificity of the immunolaheling pattern. Investigations led to the conclusions that ER and polyribosomes were involved in papain synthesis. Papain was stored in the vesicles of ER origin temporally before reorganized into laticiferous vesicles with other components of latex.
Abstract (Browse 1899)  |  Full Text PDF       
Chromosomal Ribonucleoproteins at Mitotic Metaphase in Allium sativum
Author: Wang Xue, Guo Shi-yi, Song Lin-sheng, Fan Ting-yu, Wang Xiu-ling and Zhang Zi-li
Journal of Integrative Plant Biology 1994 36(7)
    Chromosomal ultrastructure and ribonucleoproteins (RNP) at mitotic metaphase in garlic (Allium sativum L. ) were studied. Areas of low electron in density were observed in the centre of some chromosomes with double staining with Ag staining technic. These areas of low electronic density were further observed as perferated holes. With Bernhard staining technique RNP could be detected in the holes but not evenly distributed. RNA identification was further ascertained by NaOH-treatment. The amount of RNP visualized in Bernhard staining technique gradually faded as the duration of EDTA treatment was in creased.
Abstract (Browse 1849)  |  Full Text PDF       
Polyethylene Glycolª²Induced Fusion of Selected Pairs of Single Protoplasts
Author: Sun Meng-xiang, Yang Hong-yuan and Zhou Chang
Journal of Integrative Plant Biology 1994 36(7)
    A new method for polyethylene glycol (PEG) -induced fusion between single pairs of selected protoplasts was developed. The protoplasts were prepared from tobacco leaves. Under an inverted microscope two defined protoplasts were selected with a hand-made micropipette and transferred into a droplet of fusion solution containing 25 % PEG (M. W. 6000), 0. 1 mol/L mannitol and 0. 01 mol/L CaCl2 ¡¤ 2H2O (pH 5.6). Slightly moving the pipette caused the protoplasts to contact and adhere to each other, the fusion pairs were then transferred to a solution containing 10% PEG, 0.35 mol/L sucrose and 0. 01 mol/L CaCl2 ¡¤ 2H2O (pH 5.6) for approximately 10 min, followed by subsequent washing with a solution containing 0.45 mol/L sucrose and 0.04 mol/L CaC12 ¡¤ 2H20 (pH 7¡ª9). Compared with conventional fusion methods adopted to protoplast population, the present method can avoid either blind fusion of protoplasts belonging to one partner and fusion among multiple protoplasts, or the presence of unfused protoplasts, thus ensure the fusion to be precisely at the level of a selected pair of single protoplasts. Moreover, it is simple and convenient enough to show its potentiality for wide application in somatic hybridization and particularly in the case of small quantity of parental protoplasts such as in vitro intergametic fusion studies.
Abstract (Browse 1806)  |  Full Text PDF       
The Chemical Constituents of Goniothalamus howii (¢ò)
Author: Yang Ren-zhou, Zhang Lian-long and Wu Shu-jun
Journal of Integrative Plant Biology 1994 36(7)
    Crystal V, VI and VII were isolated from the seeds of Goniothalamus howii Merr. Et Chun. Based on the analysis of IR, 1H-NMR, 13C-NMR, MS and spectral analysis of their derivatives, they were identified as a new type of annonaceous acetogenins¡ªseco-biste-trahydrofuran annonaceous acetogenins. V and VI were named as howiicin D and howiicin E, respectively. VII is a mixture of two compounds, named howiicins F and C, which have the same plane structure.
Abstract (Browse 1868)  |  Full Text PDF       


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