September 1998, Volume 40 Issue 9


          Research Articles
Effect of PEG and Salt on Crystallization of Bacterioferritin and Nitrogenase MoFe Protein from Azotobacter vinelandii
Author: HUANG Ju-Fu, WANG Dao-Yong, Wang Zhi-Ping, LUO Ai-Ling, GU Shu-Rong and LI Jia-Ge
Journal of Integrative Plant Biology 1998 40(9)
    MgCl2 was added to the supernatant of the first crystallization of MoFe protein to give a final concentration of 14.6 mmol/L, followed by centrifugation. The treated supematant solution and MoFe protein could be crystallized by using method of siting drop with PEG 6000 and MgC12 as a precipitant and salt, respectively. The larger crystal from the supermatant was observed when the final concentration of PEG and MgCl2 was 4.5% and 15.6 mmol/L, respectively; but small crystal was observed when the concentration was 0 and 23.8 mmol/L, respectively. The larger crystal in brown rectangular prism of MoFe protein was also obtained using the same crystallization method when the final concentration of PEG and MgCI2 was 7.44% and 338.0 mmol/L, respectively. It suggests that the two protein crystals seem to be different, the former being bacterioferritin and the later as nitrogenase MoFe protein.
Abstract (Browse 1806)  |  Full Text PDF       
On the Evolution of the Palynoflora and Climatic Environment During Late Pleistocence in Tengger Desert, China
Author: MA Yu-Zhen, ZHANG Hu-Cai, LI Ji-Jun, Pachur H J and Wnnemann B
Journal of Integrative Plant Biology 1998 40(9)
    Palynoflora studies on the Duantouliang section, located at 39º40'N, 103º55'E in Northwestern Tengger Desert, China showed that, based on the spore-pollen assemblages, the major vegetation and climatic environment between 42 000 to 23 000 a BP could be divided into the following different periods: . From 42 000 to 38 000 a BP, the spore-pollen assemblages displayed that the mixed conifer/deciduous broad-leaved forests developed on the mountain and its foothill regions where the bare Gobi-desert are at present; At that time, Populus and Salix forests and grassland surrounded the Paleaolake, the climate condition was much warmer and humid than today; . From 38 000 to 37 000 a BP, the climate was warm and moist, it was the most suitable period for the plant growth, the studied area was dominated by the temperate and warm-temperate mixed broad-leaf deciduous and needleleaf forest, there was meadow spreaded on the river sides and lake beaches; . From 31 000 to 30000 a BP, the needleleaf forests and cold-temperate Salix oritrepha shrubs were flourishing, and the climate at that time was relatively cold;. From 30000 to 28000 a BP, the temperatures began increasing, the high lake levels was formed during this time, and the vegetations were meadows and swamps; V. From 28 000 to 23 000 a BP, temperate Cupressaceae and Betula mixed conifer/deciduous forests grew on mountain and foothill region, grassland developed on plain areas, Salix was on lake and river sides. This indicates a warm and moist climate condition but it was drier than the earliest period.
Abstract (Browse 1888)  |  Full Text PDF       
The Community Types and Biomass of Sibiraea angustata Scrub and Their Relationship with Environmental Factors in Northwestern Sichuan
Author: WU Ning
Journal of Integrative Plant Biology 1998 40(9)
    Sibiraea angustata (Rehd.) Hand.-Mazz. scrub is a kind of typical and representative community on the eastern Qinghai-Tibetan Plateau, and is distributed on the subalpine and alpine belts in northwestern Sichuan with the latitude between 31~ and 34~ 47 quadrats were surveyed in the representative distributionarea of Sibiraea angustata scrub in order to probe into its types, characteristics, relationship with environmental factors and biomass structure. Combined with the quantitative classification, the result showed that Sibiraea angustata formation in the northwestern Sichuan could be divided into 4 association groups and 10 association types. Furthermore, the analysis of PCA (principal component analysis) ordination determined that the distribution of the plant communities was closely related to the moisture and organic matter content in the soil and the vertical change of heat. Meanwhile, the lowest classification unit of the community-association was most susceptible to the variation of environmental factors. The research of biomass expressed that the total biomass of the Sibiraea angustata community fluctuated between 38.99 thm- 2 and 47.72 thm-2, among which the biomass of the shrub layer and herb layer were 25.87- 32.16 thm-2 and 12.05 - 13.73 thm-2 respectively. Generally speaking, it could be concluded that on the high altitude area the biomass of the aboveground was more than that of the underground, such as the underground biomass of Sibiraea angustata community was 19.04% C42.72% more than that of the aboveground.
Abstract (Browse 1847)  |  Full Text PDF       
Foliar 13C Within Temperate Deciduous Forest: Its Spatial Change and Interspecies Variation
Author: YAN Chang-Rong, HAN Xing-Guo, CHEN Ling-Zhi, HUANG Jian-Hui and SU Bo
Journal of Integrative Plant Biology 1998 40(9)
    The stable carbon isotope ratios (13C) of dominant tree and shrub species in typical temperate broad-leaved forests in Dongling Mountain, northwestern part of Beijing, China, were studied. The results indicated that the values of foliar 8 13C differed among the species tested, in the following order of Prunus armeniaca var. ansu Maxim. > Fraxinus rhychophylla Hce. Virex negundo var. heterophylla (Franch.) ReM. Quercus liaotungensis Koiz. Syringa pekinensis Rupr. > Juglans mandshurica Maxim. The 13C values were also affected by the maturity of leaves. The newly-developed leaves had higher 13C values than the mature ones, especially in Juglans mandshurica and Quercus liaotungensis. The conditions of habitats, particularly soil depth and moisture content, also had pronounced effect on foliar 13C. The foliar 13C values of plants grown in dry habitats were higher than those in moist ones. They were also affected by the position of leaves in the canopy: leaves at upper canopy showed higher 13C values than the lower ones. It is suggested that stable isotope technique may be effective for the study of ecophysiology of plants in the warm temperate regions of China.
Abstract (Browse 2047)  |  Full Text PDF       
The Chemical Components of Essential Oils from the Leaves of 110 Species and Cultivars of Citrus Plants
Author: HUANG Yuan-Zheng and CHEN Quan-You
Journal of Integrative Plant Biology 1998 40(9)
    Seventy-two chemical components of essential oils from the leaves of 110 species and cultivars of Citrus were analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). Among the plants analyzed, there are 3 species in papeda, 12 species and cultivars in Osmocitrus, 18 cultivars in C. aurantium L., 15 cultivars in C. sinensis Osbeck, 16 species and cultivars in Citrophorum, 16 species and cuhivars in Cephalocitrus, 30 species and cultivars in Acrumen. As resources of essential oils, some valuable plants were found such as Citrus hystrix D. C., C. junos (Sieb.) Tan. cv. Xiecheng, C. junos (Sieb.) Tan. cv. Luohancheng and C. tankan Hayata. Our study has provided systematic data of the chemical components of the essential oils for the taxonomic work of Citrus plants.
Abstract (Browse 1873)  |  Full Text PDF       
Cell Growth and Flavonoids Production in Suspension Culture of Saussurea medusa
Author: XING Jian-Min, ZHAO De-Xiu, LI Mao-Yin, YE He-Chun, LI Guo-Feng and LI Zuo-Hu
Journal of Integrative Plant Biology 1998 40(9)
    Cell suspension cultures were established from Saussurea medusa Maxim. callus cultures. The effects of different rotation speeds of the gyratory shaker, different inoeulum sizes and different pH values of the medium on cell growth and flavonoid formation were studied. The result showed that the optimum rotation speed, inoeulum size and initial pH value of the medium were 90C120 r/min,50C 80 g FW/L and 5.5C6.0 respectively for cell growth and flavonoids formation in the suspension cultures. Sucrose was better than glucose and fructose for the suspension cultures. The optimum concentration of sucrose for cell growth and flavonoid production was 40 g/L, and the concentration of flavonoids could be as high as 1 423.25 mg/L. High performance liquid chromatographic analysis of cell suspension culture extracts showed that the concentrations of jaceosidin and hispidulin in the flavonoids were 22.11% and 0. 15% respectively.
Abstract (Browse 2175)  |  Full Text PDF       
Technical Factors of Artemisin Biosynthesis in Artemisia annua Hairy Root Culture
Author: LIU Chun-Zhao, WANG Yu-Chun, OUYANG Fan, YE He-Chun and LI Guo-Feng
Journal of Integrative Plant Biology 1998 40(9)
    The optimum cultural conditions of growth and artemisinin accumulation of hairy roots in Artemisia annua L. were identified as follows: the initial pH 5.8 ~ 6.0; the rate of flask shaking, 130 ~ 150 r/min; the liquid medium volume per flask, 25%; light cycle, 16 h/d; temperature, 30 . Under the above conditions, up to 233.3 mg/L of artemisinin could be obtained after 25 days of culture.
Abstract (Browse 1838)  |  Full Text PDF       
Establishment of an Index to Appraise Cold-resistant Breeding for Citrus in Early Stage
Author: LI Wei, SUN Zhong-Hai, ZHANG Wei-Cai and LIU Dao-Hong
Journal of Integrative Plant Biology 1998 40(9)
    Cold-resistance of citrus protoplasm and field plants in 5 different varieties was studied. The relative analysis between cold resistance of citrus protoplasts and that of field plants indicates that the cold resistance of citrus protoplasm can be considered as an index of cold resistance of field plants.
Abstract (Browse 1837)  |  Full Text PDF       
Purification and Characterization of the Millettia pachycarpa Lectin
Author: ZENG Zhong-Kui, DENG Jun-Lin and BAO Jin-Ku
Journal of Integrative Plant Biology 1998 40(9)
    A lectin with strong hemagglutinating and mitogenic activity was isolated from the seeds of Millettia pachycarpa Benth. by extraction, fraction with (NH4)2 804, ion-exchange chromatography on DEAE-Sepharose and followed by gel filtration on Sephadex G-100. The purified leetin showed a single protein band on PAGE and SDS-PAGE, and exhibited a molecular weight of 40 700 by gel filtration and subunit of 19 800 on SDS-PAGE. It contained 1.78% neutral saceharide and enriched Asp, Glu, Thr, Set, Leu and also contained 4 Trp per molecule. It agglutinated rabbit red cell at 0.48 g/mL and A,B,O types of blood. The reaction could be inhibited by thyroglobulin, muein gastrie and ovomucin, but not by saceharide. The hemagglutination was strongly dependant on Ca2 +, but was not enhanced by Mg2 + ,Mn2 + ,Zn2 +; The lectin was a strong mitogen for human peripheral blood lymphoeytes, showing a transformation rate and mitotic index of 84.3 % and 7.8 %,respectively.
Abstract (Browse 1787)  |  Full Text PDF       
Overexpression of petH Gene of Cyanobacterium synechococcus sp. PCC 7002 in Escherichia coll and Purification of the Expressed Product
Author: LI Rong-Gui, ZHAO Jin-Dong, WU Guang-Yao and WU Xiang-Yu
Journal of Integrative Plant Biology 1998 40(9)
    Fd:NADP+ oxidoreductase (FNR) is one of the key enzymes in photosynthetic electron transport. The gene petH encoding FNR of Synechococcus sp. PCC 7002 was cloned into the expressing vector pET-3 d' and overexpressed in E. coli. The amount of recombinant FNR (rFNR) was over 50% of the total cellular proteins. There were two forms of FNR activity, one is soluble and the other one was in the form of inclusion bodies. The soluble rFNR was purified through ion exchange chromatography and gel chromatography. The rFNR in the form of inclusion bodies was first solubilized with 6.7 mol/L urea, and then refolded into the active form in the presence of flavin adenine dinucleotide (FAD). Further purification was performed by ion exchange chromatography. The rFNR pmified from either form of the expressed product had the maximum absorption spectrum as that of the natural FNR from cyanobacteria, whose maximum absorption was at 273, 385 and 456 ran respectively. N-tenninal sequencing showed that rFNR was indeed a product of petH gene expression, rFNR could catalyze the electron transport from P700 to NADP+ in the presence of ferredoxin. The optimal pH for diaphorase activity of rFNR was 8.0 and the optimal temperature was 30 .
Abstract (Browse 1892)  |  Full Text PDF       
An Intron Sequence is Present in the 3 UTR of Tomato Cf9 Gene
Author: YANG Chong-Lin, WANG Min, LI Yi, QU Li-Jia, GU Hong-Ya and CHEN Zhang-Liang
Journal of Integrative Plant Biology 1998 40(9)
    Tomato leaf mould-resistance gene Cf) was amplified by PCR and cloned from the genomic DNA of a tomato ( Lycopersicon esculentum Mill. ) cultivar ("Zhongza 9'). Sequence analysis indicates that this Cf9 gene is 2 751 bp long and contains an open reading frame encoding a protein of 863 amino acids. An intron of 115 bp in length is found in the 3' untranslated region of this gene. The exon/intron borders are replicate sequences, TCCAGG(T)ATTC, which shares high homology with those of the two genes of Cf2 which is another leaf mould-resistant gene locus in tomato. Compared with the previously reported cDNA sequence of Cf9, a single change of T to C occurs at the nucleotide position of 371 in this PCR-ampliiied Cf) gene, which converts Leu 121 to Pro in the LRR region of the encoded protein.
Abstract (Browse 2146)  |  Full Text PDF       
Rice Transformation with a Phytoalexin Gene and Bioassay of the Transgenic Plants
Author: TIAN Wen-Zhong, DING Li, CAO Shou-Yun, DAI Shun-Hong, YE Song-Qing and LI Liang-Cai
Journal of Integrative Plant Biology 1998 40(9)
    Immature embryos, mature embryos and embryogenie ealli of 6 rice ( Oryza sativa L. ) materials were transformed with particle bombardment. The plasmids pSSVsfl and pVE5 + were used, both containing the phytoalexin gene from grapevine coding for stilbene synthase, but driven by 35S and its own promoter respectively. Through resistance selection for G418 ( 100 to 150 mg/L) or hygromycin (50 mg/L), 54 independent transgenic plants were isolated and further assessed by PCR, Southern blot and Dot blot analyses. The transgenic plants and their progenies were tested for resistance to blast ( Pyricularia oryzae) and bacterial blight of rice ( Xanthomonas oryzae). Preliminary results indicated that the stilbene synthase gene could enhance the resistance of transgenic plants and their progenies to both pathogens.
Abstract (Browse 2142)  |  Full Text PDF       
Study on Regulation of 5 Flanking Regions of Granule-bound Starch Synthase Gene of Potato
Author: SONG Dong-Guang, SUN Guo-Feng, SHAN Hai-Yan, PENG Xiang-Yin, WANG Guang-Qing, WANG Xun-Ming and TAN Jia-Zhen
Journal of Integrative Plant Biology 1998 40(9)
    Binary vectors were constructed by fusing 0.4, 0.8, 1.6, and 2.9 kb 5' flanking regions of GBSS gene with GUS (-glucuronidase). Transient GUS expression was observed in in vitro tuber slices bombarded with 0.8 kb GBSS-GUS construct. These constructs were then transferred into potato ( Solanum tuberosum L. cv. Desiree) via Agrobacterium tumefaciens transformation. Transgenic potato plants were confmned by X-Gluc staining and PCR. Using in vitro tuberization system, GUS expressions were assayed with fluorescence, it was shown that 0.8, 1.6 and 2.9 kb GBSS-GUS expressions were higher than 0.4 kb GBSS-GUS. 1.6 and 2.9 kb GBSS-GUS expressions were about 2 to 10-folds higher in tubers than in stems. In cultured shoots, GBSS-GUS expression could be induced by increased sucrose concentration but inhibited by light.
Abstract (Browse 1858)  |  Full Text PDF       
Study of Intermediate Filaments in Adiantum philippense and Comparative Analysis of Karetin-like Proteins in Some Plant Species
Author: CHEN Dan-Ying, ZHAO Yun, ZHAO Da-Zhong and ZHAI Zhong-He
Journal of Integrative Plant Biology 1998 40(9)
    Network of filaments, 10 nm in diameter, was detected in the frond cells of Adiantum philippense L. by selective extraction combined with whole mount electron microscopy. Western blot analysis showed that the major filament components were cross-reacted with monoclonal antibodies against animal keratin. The phenomenon was in concert with the result of indirect immunofluoreseenee observation. The filaments with 10 nm in diameter could be reassembled in vitro. Using selective extraction, proteins were prepared from cells of Spirulina subtillissima Kutz., thalli of Marchantia polymorpha L., and leaf blades of A. philippense L. , Ginkgo biloba L. and Brassica pekinensis Rupr. Western blot showed that S. subtillissima Kutz. has two acidic keratin-like pro- teins and all of the other four plant species have three basic keratin-like proteins and three acidic keratin-like proteins. These results may suggest the existance of an intermediate filament network with keratin-like proteins in the cells of pteridophyte and their existance in plant cells is popular.
Abstract (Browse 1976)  |  Full Text PDF       
Observation on Rice Embryo Sac Development with Confocal Laser Scanning Microscopy
Author: REN Hong, LIU Yong-Sheng and SUN Jing-San
Journal of Integrative Plant Biology 1998 40(9)
    An easy and rapid procedure has been launched for observafion of embryo sac development of Oryza sativa L. Following fixation and dehydration, whole mounted ovaries were cleared in methyl sulieylate, finally sealed under coverslips with clove oil. With the confocal laser scanmng microscopy procedure, the embryo sac exhibited autofluorescence and could be clearly observed. FAA and 4% glutaraldehyde as fixative produced different results but the latter seemed to be better.
Abstract (Browse 2199)  |  Full Text PDF       
The Pollination Syndrome of Pedicularis rex (Scrophulariaceae) and Its Biogeographic Significance
Author: WANG Hong
Journal of Integrative Plant Biology 1998 40(9)
    The pollination biology of different populations of Pedicularis rex Clarke C B. was studied in Zhongdian of Northwest Yunnan and in Kunming of central Yunnan, respectively. Seed production depends exclusively upon pollination by bmnblebees ( Bombus Latr. ). The flowers are yellow, with short-tubed, erostrate and nectarless corolla. Two species of the bumblebee workers, Bombus friseanus Skorikov and B. lucorum L. foraged in an upright (nototribic) position for pollen. Meanwhile, Bombus friseanus workers also foraged in an inverted (stemotribic) position on P. rex, vibrating pollen from the anthers within the galea. This pollination syndrome was previously reported in the North American species, e.g.P, labradorica which has the same corolla-type with P. rex. A comparison of the corolla types and pollination syndrome on the species of Pediadaris in East Asia and North America indicates that such a pollination syndrome may be only found in the primitive corolla type of Pedicularis. In spite of the separation in the geological history, P. rex and P.labradorica share the same corolla type and pollination syndrome. Further study is needed to elucidate the biogeography of the Pedicularis.
Abstract (Browse 1939)  |  Full Text PDF       
Structural Identification of Huperzine G
Author: WANG Bao-De, JIANG Shan-Hao, GAO Wen-Yun, ZHU Da-Yuan, KONG Xiang-Ming and YANG Yi-Qing
Journal of Integrative Plant Biology 1998 40(9)
    A new lycopodium alkaloid, huperzine G was isolated from the n-butanol fraction of Huperzia serrata (Thunb.) Trev. The structure of this alkaloid was determined through IR, MS, ID-and 2D-NMR spectral analyses.
Abstract (Browse 1886)  |  Full Text PDF       


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