January 1999, Volume 41 Issue 1


          Research Articles
Gene Expression Analysis of a Proline-rich Protein from Bean Under Biotic and Abiotic Stress
Author: CHAI Tuan-Yao and ZHANG Yu-Xiu
Journal of Integrative Plant Biology 1999 41(1)

The PvSR1 ( Phaseolus vulgaris stress-related protein) gene encoding an 11 kD proline-rich protein (PRP) was constitutively expressed in the primary leaf tissue of bean ( Phaseolus vulgaris L. cv. Saxa). Time course studies of mRNA accumulation of the PvSR1 has been studied in this plant against virus infection and various abiotic stresses. Northern blot analysis revealed that the gene expression of PvSR1 was greatly enhanced in the leaf tissue by A1MV (alfalfa mosaic virus) infection, wounding, heat shock, tN, drought and salt stress. The PvSR1 transcripts could also be increased by the exogenous inducing factors such as salicylic acid and 11202. The precise biological roles of PvSR1 is unknown, but the expression of PvSR1 genes in various form of stress suggest that PvSR1 may play an important role during the stress, in maintaining cellular integrity by forming strong hnkages with the cell wall.

Abstract (Browse 1838)  |  Full Text PDF       
Chemical Constituents of the Anemone tomentosa Root
Author: WANG Jun-Ru,PENG Shu-Lin, WANG Ming-Kui, FENG Jun-Tao and DING Li-Sheng
Journal of Integrative Plant Biology 1999 41(1)
    The roots of Anemone tomentosa (Maxim.) Pei (Ranunculaceae) have been used as traditional medicine for the treatment of dysentery, infantile malnutrition, carbuncles, etc. Ethyl acetate extract of the roots displaied stronger antifeedant activity against Leucania separata Walker. Eleven compounds were isolated by silica gel column chromatography from the extract and their structures were identified as 4,5-dimethoxyl-7- methyl-coumarin(1),4-methoxyl-5-methyl-6,7-methylenedioxy-coumarin (2), 4, 7-dimethoxyl-5-methyl-comnarin (3), oleanolic acid (4), oleanonic acid (5), 3-O-¦Â-D-xylopyranosyl oleanolic acid (6),¦Â-sitosterel (7), stigmasterol (8), daucosterol (9), 3-O-¦Â-D-glucopyranosyl stigmasterol (10) and ergosterol peroxide (11) respectively on the basis of spectral evidence. Compounds 1 and 2 are new natural products.
Abstract (Browse 1940)  |  Full Text PDF       
Spores and Pollen from Crude Oils of Weizhou Oilfield in Beibu Gulf of South China Sea
Author: JIANG De-Xin and YANG Hui-Qiu
Journal of Integrative Plant Biology 1999 41(1)
    Fifty-eight species of fossil spores and pollen referred to 28 genera and 3 species of fossil algae referred to 2 genera extracted from the cmde oil samples were recorded. The samples were collected from the reservoirs within the Lower Liushagang Formation of the Weizhou Oilfield in the Southwest Weizhou Depression of the Beibu Gulf Basin. Based on the comparisons between palynomorphs in crude oils and palynomorphs in potential source rocks, the petroleum source rocks of the petmliferous region were determined. According to the paleoecological characteristics of the original plants producing the spores and pollen, the sedimentary enviromnent of the petroleum source rocks was discussed. The results of the study indicate that the Middle Eocene to Lower Oligocene Liushagang Formation should be the main petroleum source rock series of the Beibu Gulf Basin, and the Middle to Upper Oligocene Weizhou Formation should also contain favorable petroleum source rocks; the petroleum source rocks might have formed in the subtropic lacustrine sedimentary environment under warm and damp climatic conditions.
Abstract (Browse 1835)  |  Full Text PDF       
Spatial Distribution Pattern of Cathaya argyrophylla Population
Author: XIE Zong-Qiang, CHEN Wei-Lie, LIU Zheng-Yu, JIANG Ming-Xi and HUANG Han-Dong
Journal of Integrative Plant Biology 1999 41(1)
    Cathaya argyrophylla Chun et Kuang is a Chinese endemic rare species distributed in Sichuan, Guizhou, Guangxi and Hunan Provinces. It is considered as an endangered crop and is listed in the China Red Data of Plants due to its very limited resources. The distribution pattern of C. argyrophylla population was related to its biological characteristics such as the age structure and regeneration mode, and the microenvironmerits. In the old forests, gaps were abundant and occupied by variable-aged individuals of C. argyrophylla, resulting in a contagious distribution pattern. In the young forests random distribution pattern was more often found. Under the same climatic and site condition, the heterogeneity of microenvironments resulting from the different degree of rock cover and soil erosion significantly affected the type and scale of distribution pattern of C. argyrophylla population. Even in the same gap, the center and the edge could have different effects on the distribution pattern due to the uneven sunlight illuminance. As one of the codominants, the distribution of C. argyrophylla in communities was affected by other dominants, especially by the evergreen tree species. Light was the major factor determining the distribution pattern of C. argyrophylla in various communities. The pattem scale of contagious distribution of C. argyrophyUa population was mostly less than 16 ©O, although it differed among communities.
Abstract (Browse 2132)  |  Full Text PDF       
An Approach to Estimating Respiration of Forest Community and Its Application
Author: FANG Jing-Yun
Journal of Integrative Plant Biology 1999 41(1)
    A method for estimating forest community respiration based on the morphological features of the trees and structural characteristics of forest was described. The method mainly involved four procedures, viz. establishment of 1 ) an appropriate relationship between the diameter class and the lengths, i.e. an equation to access the diameter distribution function, of woody organs; 2) a quantitative relationship between the respiration rate and the diameter of woody organs; 3) a model with biological significance for estimating the forest respiration; 4) equations for estimating the forest community respiration. The method has been used to estimate community respiration of three temperate forest types in the mountain of Beijing, Pinus tabulaeformis Carr., Betula platyphylla Suk., and Quercus liaotungensis Koidz. forest. The mean respiration rates of these three forest types were between 1 256 and 1 491 mg CO2 (tree • h)-1, showing no clear difference among them. Their annual community respiration rate was estimated to be 15.5, 8.0 and 4.9 tones dry weight (hm2 • a) -1, respectively, indicating close values to most temperate forests which have been reported around the world.
Abstract (Browse 1805)  |  Full Text PDF       
Role of Simulated Acid Rain on Acer negundo and Plant Nitrition
Author: DU Ying-Lei, Christophe N Nsoukpo¨¦-Kossi,Raymond B¨¦langer and Roger M Leblanc
Journal of Integrative Plant Biology 1999 41(1)
    The short-term effects of simulated acid rain on Acer negundo L. have been investigated using photoacoustic spectroscopy and modulated fluorescence measurements. Three seeding groups of Acer negundo L. were treated with simulated acid rain at pH 3.0, 4.5 and 5.6 levels for three months. The results indicated the photosynthetic activity, expressed in terms of normalized oxygen evolution signal, photochemical energy storage, normalized variable fluorescence (Fv/Fm), photochemical and non-photochemical chlorophyll fluorescence quenching, ranges in the order pH 4.5 > pH 3.0 > pH 5.6. This stimulation effect has been confirmed by increased growth rate observed in relation with increased acidity levels. The nutritional aspects of this favorable effect of the acid rain on plant growth were discussed.
Abstract (Browse 1788)  |  Full Text PDF       
Dynamic Changes of Endogenous Plant Hormones in Rice Grains in Different Parts of Panicle at Grain filling Stage
Author: DUAN Jun, TIAN Chang-En, LIANG Cheng-Ye, HUANG Yu-Wen and LIU Hong-Xian
Journal of Integrative Plant Biology 1999 41(1)
    The grain fresh weight and grain-filling rate at early grain developing stage in rice ( Oryza sativa L. ) were higher in the upper than in the lower part of panicle during grain filling. The grains in the upper and/or the lower part of panicle were analyzed for the endogenous plant hormones. The results indicated that the peak content of ABA and/or GA1 + 3 in the developing grains appeared at about the same stage when the highest increasing rate of grain appeared in the upper and in the lower part of the panicle respectively. After heading, the IAA content in developing grains steadily decreased in both the upper and the lower part of the panicle, GAn + 7 content decreased at first and then increased, and again decreased. At the early grain-filling stage, the ABA and GA1 + 3 contents in the developing grains were higher in the upper than in the lower part of the panicle. The role that the hormones play in the developing grains and the reason why the grains seed set better in the upper than in the lower part of the panicle were discussed.
Abstract (Browse 1766)  |  Full Text PDF       
Characteristics and Crystallization of Nitrogenase MoFe Protein from a nif Z Deleted Strain of Azotobacter vinelandii
Author: WANG Dao-Yong, WANG Yao-Ping, HUANG Ju-Fu,WANG Zhi-Ping, ZHONG Ze-Pu and LI Jia-Ge
Journal of Integrative Plant Biology 1999 41(1)
    ˇ÷nifZ MoFe protein purified from a nifZ deleted strain of Azotobacter vinelandii (DJ194) was shown to be pure by SDS-Polyacrylamide gel electrophoresis. The protein contained 1.5 Mo atoms and 15.9 Fe atoms per molecule, the ratio of Fe to Mo was lower than that of the MoFe protein purified from the wild type strain of A. vinelandii; and Call2, H+ -reduction activity and their ratio (C2H4/H2 (Ar)) were 16.6%, 21.7% and 77.2% of those of the wild type MoFe protein, respectively. Under a somewhat different condition from that for the crystallization of the wild type MoFe protein dark brown rhombohedron crystals of ˇ÷nifZ MoFe protein were obtained. It indicated that the deletion of the ˇ÷nif Z resulted in the decrease of number or change in the structure of P-cluster in the mutant MoFe protein, which caused the significant structured and function of change of the protein.
Abstract (Browse 1748)  |  Full Text PDF       
Establishment of a Highly Efficient Ammonia Secreting Mutant of Synechococcus sp. PCC 7942 and the Glutamine Synthetase Activity, Photosynthesis and Growth in Its Immobilized Cells
Author: QIN Jing-Dong,SHAO Ning, SHI Ding-Ji, XU Xu-Dong, ZHANG Jin-Dong, GUO Ping-Zhong,WANG Wen-Qing and TANG Pei-Song
Journal of Integrative Plant Biology 1999 41(1)
    A recombinate plasmid pDC-ATGS was constructed, which contained the antisense fragment of glnA gene from Anabaena sp. PCC 7120 and transformed the unicellular cyanobactefium Synechococcus sp. PCC 7942. The foreign DNA was inserted into the site of glnA locus of the chromosome through the homologous recombination. By using neomyisin, a highly efficient ammonia secretion mutant was selected. After immobilized, the cells of the mutant within polyurethane (PU) foams, glutamine synthetase (GS) and NIt4+ secretory activity of GS, and its growth and photosynthesis were measured. It was shown that NH4+ secretion of the immobilized mutant was enhanced 156 folds which was much higher than that of free-living cells of the wild type. The activity of GS was decreased by 73.6%. Growth of the mutant was the same as that of the wild type. The activity of photosystem ˘ň in the immobilized mutant cells increased by 44% with 77 K fluorescence spectrum measurement.
Abstract (Browse 1785)  |  Full Text PDF       
Energy Transfer from Cyanobacterial Phycocyanins to Chlorophyll a containing Liposomes
Author: YU Guo-Qiang, ZHANG Hui and RONG Shou-Yu
Journal of Integrative Plant Biology 1999 41(1)
    The phycocycins (PC) from the thermophilic cyanobacterium Myxosarcina concinna Printz and the Chi a-containing liposomes were prepared and their spectral characteristics were measured before the energy transfer from PC to Chi a-containing liposomes was studied. It was found that the efficiency of light energy transfer was decreased when the surface of the vesicle bilayer was negatively charged. It was relatively increased when the membrane was incorporated with the positively-charged surfactaht, dioctadecy-ldimethylammonium chloride (DODAC), of increasing concentration from 0 to 30 mol%. These results indicated that the electrostatic force might have great effects on the energy transfer from phycocycins to Chi a-containing liposomes.
Abstract (Browse 1799)  |  Full Text PDF       
RFLP Variations of Common Wheat Doubled Haploid Progenies from WheatˇÁMaize Crosses
Author: CHEN Chun-Xian, SUN Jing-San and ZHU Li-Huang
Journal of Integrative Plant Biology 1999 41(1)
    Wheat ( Triticum aestivum L. ) and maize ( Zea mays L. ) crosses (the chromosome elimination system) can be used to produce frequently a large number of doubled haploid (DH) wheat lines by embryo rescue and doubling treatment. The resulting DH lines are genetically homogeneous. Significant RFLP variations were detected in common wheat DH progenies from wheat and maize crosses by using wheat rDNA clone pta71 and two maize DNA clones (MR13 and MRSO) homologous to wheat genome as probes. The results revealed that the copy number and restriction fragment length of rDNA in some wheat DH progenies was changed, and also that deletion was detected in several DH plants when probed with MR13 and MR5O. In particular, the RFLP pattern of DH line No. 18 was greatly changed using MR13 as a probe. In this line, three new bands, 40.0 kb, 2.5 kb and 2.0 kb emerged while a 4.3 kb intense band from the parental common wheat genome disappeared. This change may be related to a quite large DNA rearrangement within the wheat genomic DNA or an insertion by alien maize DNA fragment.
Abstract (Browse 1747)  |  Full Text PDF       
Analysis of the Maize 19 kD Zein Gene Promoter Fragment Driving Seed specific Expression
Author: ZHAO Qian, LIANG Hua, MA Chong-Lie and AO Guang-Ming
Journal of Integrative Plant Biology 1999 41(1)
    A deletion works of a maize 19 kD zein gene promoter in the 5'end was performed and six promoter fragments of different length were obtained. A series of expression vectors was constructed and then transferred into tobacco ( Nicotiarta tabacum L. ) plants. GUS activity assays indicated that the expression of 488 bp promoter was tissue-specific, for which GUS was active only in transgenic tobacco seeds. The other four fragments containing 378 bp,302 bp,152 bp and 124 bp also have the activity of promoter. They could drive gus gene expressed not only in seeds but also in roots and petioles.
Abstract (Browse 1946)  |  Full Text PDF       
Cloning of the Homeotic Gene fbp2 from Petunia hybrida and Its Effects in Tobacco Flower
Author: YU Jing-Juan,GUO Feng-Li, ZHAO De-Gang, FU Yong-Fu, HAN Yu-Zhen, AO Guang-Ming and MENG Fan-Jing
Journal of Integrative Plant Biology 1999 41(1)
    Messenger RNAs were isolated from the petunia (Petunia hybrida L. ) flower buds and cDNAs were synthesized from reverse transcription. The petunia homeotic gene fbp2 was amplified by PCR using cDNAs as templates. Sequence analysis indicated that the isolated fragment was composed of 686 bp and the region flanked by two primers showing 99.6% homology to the sequence reported by Angenent et al. Three bases changed, and MADS domain was conserved. Using petunia flower homeotic gene ibp2 cDNA( yfbp2 ) as target gene, the expression vector pBBP2 containing CaMV 35S promoter/yfbp2/nos terminator was constructed and was transformed into Agrobacterium tumefaciens strain LBA4404 pAL4404) by direct transformation method. Incubating the leaf explants of tobacco (Nicotiana tabacum L. var. samsum ) with LBA4404 (pAL4404) and selecting in the medium containing 100 mg/L kanamycln, the regenerated resistant plants were obtained. Polymerase chain reaction and Southern blotting demonstrated that the target gene was integrated into the genome of tobacco. The flowers of the transgenic tobacco were aberrant resulted in expression of fbp2, with petal formed on the stamen.
Abstract (Browse 1943)  |  Full Text PDF       
Isolation and Characterization of a cDNA Encoding Maize Cyto solic Malate Dehydrogenase
Author: HU Jian-Guang, ZHAO Xiang-Shan, LIU Jun, YUAN Zi-Qiang and YANG Jin-Shui
Journal of Integrative Plant Biology 1999 41(1)
    A cDNA fragment derived from a gene over-expressing in hybrid maize ( Zea mays L. ) was isolated with RT-PCR and used as probe to screen cDNA library of hybrid maize seedlings. A positive cDNA clone ZH02 corresponding to the full-length mRNA sequence was obtained, which was shown to have an open reading frame encoding 332 a.a. DNA and proteinase database search revealed that the deduced amino acid sequence of ZH02 has high similarity with the cMDH of Mesembryaathemum crystallium L. and Arabidopsis thaliana (L.) Heynh. up to 90% and 84%, respectively. This is the first report of the full-length gene coding for the cereal cMDH.
Abstract (Browse 1935)  |  Full Text PDF       
Molecular Cloning of a Vernalization related cDNA Clone (Vrc) of Vrc79 in Winter Wheat (Triticum aestivum L.)
Author: ZHAO Da-Zhong, CHONG Kang, WAN Li, XU Ji and TAN Ke-Hui
Journal of Integrative Plant Biology 1999 41(1)
    Vernalization is an essential factor in the flowering development of cold-required plants. There is a key stage of nucleic acid metabolism in the process of vernalization in winter wheat. To probe into the molecular determinants of vernalization, a cDNA library presumably enriching vernalization-related genes was prepared by specially expressed mRNAs from winter wheat ( Triticum aestivum L. cv. Jingdong No. 1) plumules at the key stage of 21 d vernalization being recovered as cDNAs after subtraction with mRNAs from nonvernalized and devernalized plumules. One vernalization-related cDNA clone (Vrc), Vrc79, which was only expressed at the key stage of 21 d vernalization, but not at other stages of nonvernalization, 4 d vernalization and devemalization, was isolated by differential screening of the library, and shown to be a vernalization-specific clone by Northern blot. Result of homology search suggested that Vrc79 was a novel gene identified in higher plant which was different from the cold-stress-induced genes and might play an important role in the floral induction in vernalization-requiring plants.
Abstract (Browse 1920)  |  Full Text PDF       
Organogenesis and Plantlet Regeneration in Vitro of Populus euphratica
Author: GU Rui-Sheng,JIANG Xiang-Ning and GUO Zhong-Chen
Journal of Integrative Plant Biology 1999 41(1)
    The research of organogenesis and in vitro plantlet regeneration of Populus euphratica Oliver was carried out using the tender shoots from mature tree as initial explants and MS medium as the basic medium. The effects of plant growth regulators (PGR) on the regeneration were compared. The results showed that the concentration of PGR was not strictly required for the organogenesis of the excised organs and callus, but the ratio of BA to NAA was important. Calli could be induced from the excised leaves and stems cultured on the medium with 0.5 mg/L BA and 0.5 mg/L NAA. The embryonic callus could be multiplied in dark on the medium supplemented with 0.25 mg/L BA and 0.5 mg/L NAA. For the adventitious bud regeneration of the leaf and callus, supplement with 0.5 mg/L BA and 0.1 mg/L NAA was appropriate, giving a regeneration frequency of 82.9% and 100%, respectively. The suitable level of BA and NAA for the excised stem's was 0.1 mg/L and 0.01 mg/L respectively, yielding a regeneration frequency of 83 %. Rooting occurred on the MS medium with half strength of macronutrient and addition of 0.015 mg/L NAA, and the rooting rate could reach up to 86.2%. The techniques of somatic cell cloning of P. euphratica was established in vitro. The problems of deterioration of the subcultured shoots were also discussed.
Abstract (Browse 1837)  |  Full Text PDF       
Characterization of Genome and Chromosomes in Octoploid Wheat wheatgrass Amphiploid Zhong 2 Using Fluorescence in situ Hybridization and Chromosome Pairing Analysis
Author: GAO Zhi, HAN Fang-Pu,HE Meng-Yuan,MA You-Zhi and XIN Zhi-Yong
Journal of Integrative Plant Biology 1999 41(1)
    Genomic constitution of octoploid wheat-wheatgrass amphiploid Zhong 2 was analyzed by chromosome pairing and fluorescence in sim hybridization techniques. The results indicated that the octoploid wheatwheatgrass chromosomes in Zhong 2 were derived from the distant homologous genomes of wheatgrass ( Agropyron intermedium (Host) P.B. = Elytrigia intermedia (Host) Nevski = Thinotopyrum intermedium (Host) Barkworth and Dewey, and thew distant homologous genomes were not from the E geaome of T. elongatum 2x. Zhong 2 contained 12 wheatgrass chromosomes in which a pair of chromosomes was involved in translocation between wheatgrass and wheat chromosomes.
Abstract (Browse 1776)  |  Full Text PDF       
Ultrastructural Observation on Chloroplast of the Thermo sensitive Green yellow Banded Leaf Mutant in Rice (Oryza sativa ssp.indica)
Author: SHAO Ji-Rong, WANG Yu-Zhong,LIU Yong-Sheng, SUN Jing-San and XIE Rong
Journal of Integrative Plant Biology 1999 41(1)
    The green-yellow bands interrupted discolorated transverse bands on the leaves of the thermo-sensitive rice line l103s (Oryza sativa ssp. indica ) was induced by cold shock and the change of leaf color coincided with the change of chlorophyll contents. Uhrastmctural observation revealed that the chloroplast slructure in the yellow part degenerated, i.e., the number of stocks in granum abnormally decreased, thylakoid system was disintegrated and replaced by few single thylakoid, and then the whole structure of some chloroplast became vesicle-hke structure with high electronic density. Nonetheless, in the green zone of the green-yellow banded leaf, there were slight decrease in the number granum thylakoids, disordered grana arrangement in the chloroplast, and aggregation of osmiophile globule. However, it was found that these structural alternations were reversable in the process of recovery of the yellow part of the leaves into green, and the chloroplast structure renewed normally. It might be concluded that the character of green-yellow bands induced by cold shock was consequent upon a reverse process of degeneration and regeneration.
Abstract (Browse 1858)  |  Full Text PDF       
Anatomical Observation on Differentiation and Granulation Processes of Juice Sacs in Citrus grandis
Author: ZHANG Zhen-Jue, XIE Zhi-Nan and XU Wen-Bao
Journal of Integrative Plant Biology 1999 41(1)
    In Citrus grandis (L.) Osbeck, calls in the inner epidermis and subepidermal layers of carpels divided and formed the primordium of the juice sac which grew into columnar structure. There were mefistem-likecell clusters at the apex and meristem-like cell columns at the centre of file columnar structure. These ceilswere polygonal, each with a big nucleus surrounded by dense cytoplasm. The meristem-like cell colunm,which was absent in the stalk, rail longitudinaly through the centre of the columnar structure. It differentiatednear the stalk 7 weeks after anthesis. Two and a half months after anthesis, miniature of juice sac was formed.Juice sat, consisted of 4 zones, viz, epidermis, subepidermal cell zone, elongated cell zone and juice cell zone. The granulation process of juice sac included four stages. 1. The stage of healthy juice sac: At this stage the juice sac was fulfilled with juice and the sac appeared transparent. The subepidemal cells were col]enchymatuus with thichened comers. Death of pmtoplasts occurred in individual parenchyma cells. 2. Stage ofgelatination. The juice sac was transparent and filled with gelatinous juice. The collenchyma cell wall of the snbepidennal cell were completely thickened. Some cell walls in some juice sacs were lignified. 3. Stage of granulation. A portion of, if not the whole, juice sac is milky white. Walls of more cells in four zones are thickened and liguified. The walls of more cells in the four zones were thickened and lignified. 4. Late granu-lation stage: The juice sac was yellowish white in color, partly hardened and partly atrophied giving the sac inan irregular contour. Most cells of the hardened portion were lignified and most cells of the atrophied portion were dead and became oppressed. Eventually, healthy juice sac reduced, starch in the juice sac reduced and oil drops increased with the prolongation of storage time of fruits.
Abstract (Browse 1875)  |  Full Text PDF       
Comparison of Cytology, Apical Buds and Gutta Content Between Staminate and Pistillate of Eucommia ulmoides Trees
Author: WANG Bing-Wu, WANG Ya-Qing, MO Hua, LUO Li-Xin, LI Mao-Xue and CUI Ke-Ming
Journal of Integrative Plant Biology 1999 41(1)
    Eucommia ulmoides Oliv. is a typical dioecious species, a plant with an approximately equal propertion of the staminate and pistillate trees, suggesting that the gene responsible for sex determination couldbelocated on file sex chromosomes. Nevertheless heteromorphous sex chromosomes were not found in their ovary and anther cells. Homologous chromosome pairing and division regularly occurred during meiosis of the pollen mother cells and occasionally chromosome bridges, chain and ring tetmvalents were observed in a few ceils.However, the length and maximum diameter of the apical buds in the staminate trees were significantly more than those in the pistillate, trees throughout the hudding process from mid-Deoeinber to mid-April ( P < 0.01 ).In contrast, the gutta content of the leaves in the pistillate trees was significantly much nvore than that in thestanfinate trees (P < 0.01 ). And in beth staminate and pistillate trees, however, the gutta content graduallydecreased, in the same propertion between the staminate and pistillate trees, from April to October with the seasonal change and the growth of leaves. These indicated that the size of the buds and the mount of the leafgutta content in E. ulmoides were sex dependant. Since beth of the gutta content and bud-size were quantita- tire characters which were usually contrelled by multiple genes unlikely being all located on the sex chromo-somes, it might be possible that some of the important genes controlling these two characters could be linkedwith the genes responsible for sex determination.
Abstract (Browse 1848)  |  Full Text PDF       
Actin Is Localized in the Nucleolar Skeleton of Physarum polycephalum
Author: ZENG Xian-Lu, XING Miao,JIAO Ming-Da, WANG Xiao-Guang, TAO Wei and HAO Shui
Journal of Integrative Plant Biology 1999 41(1)
    Nucleoli were isolated from the interphase nuclei of Physarum polycephalum Schw. 'lhe nucleolar skeleton was obtained after DNA and most of the nucleolar proteins were extracted with DNase I 0.25 mol/L ( NH4)2SO4 and 2 mol/L NaC1. The nucleolar skeleton appeared as a fibrous network structure composed of fibres about 10 to 30 nm in diameter when observed under the electron microscope. SDS-PAGE analyses revealed about 20 polypeptides in the nucleolar skeleton, including a 43 kD pelypeptide which is equivalent to actin in molecular weight, lmmunofiuorescence observations upon slide preparations of the nueleolar skeleton labeled with anti-actin antibody showed that the nucleolar skeleton emanated bright fluorescence, indicating the existence of thc antigen, lmmunodotting assays further localized actin in the protein preparations of the nucleolar skeleton. Results of immuno-electron microscopy, with anti-actin antibody and protein A-gold as probes, indicated that gold particles were distributed all over the nucleolus of the interphase nucleus.
Abstract (Browse 1712)  |  Full Text PDF       
Genetic Mode of Exogenes in Transgenic Plants
Author: HUA Zhi-Hua and HUANG Da-Nian
Journal of Integrative Plant Biology 1999 41(1)
    The integration and expression of exogenes were reviewed in transgenic plants (To generation) as well as in their progenies. It was analyzed that the integration, expression and stable inheritance of exogenes were different in various transformation events. The disadvantages of present transfonnation methods and new transformation methods which can site specifically introduuce exogenes into receipt chromosomes with restricted copies were discussed.
Abstract (Browse 1896)  |  Full Text PDF       


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