October 1999, Volume 41 Issue 10

 

          Research Articles
Predation and Dispersal of Quercus liaotungensis Acorns by Chinese Rock Squirrel and Eurasian Jay
Author: WANG Wei and MA Ke-Ping
Journal of Integrative Plant Biology 1999 41(10)
      
    Predation and dispersal of Quercus liaotungensis Koidz. acorns by Chinese Rock squirrel ( Sciurotamias davidianus ) and Eurasian jay ( Garrulus glandarius ) were examined in a Pinus tabulaefotrais Carr. forest in Dongling Mountain. Both of them were important acorn dispersal agents. Chinese Rock squirrels frequently forage food in this forest. They consumed a small portion of atoms in situ, while they transported large quantities of atoms in their big cheek pouches. Eurasian jays have great capacity to transport acorns, although they were uncommon visitors to this forest. They carry acorns in their sublingual pouches to other forest. Of the 18 acorns seatterhoarded by a Chinese Rock squirrel, only 3 remained in two different caches 111 days after they were cached. These results indicate that vertebrates play an important role in acorn dispersal and seedling establishment of Quercus liaotungensis.
Abstract (Browse 2008)  |  Full Text PDF       
A Preliminary Study on the Function of Ethylene Induced 60 kD Protein in Carnation Petal
Author: JIANG Wei-Bo, Shimon MAYAK, Abraham H. HALEVY
Journal of Integrative Plant Biology 1999 41(10)
      
    Antibody against the ethylene induced 60 kD protein (E60) in camation( Dianthus caryophyllus L. ) petal was obtained from immunized rabbit serum. The E60 was purified with gel filtration, DEAE- Sephadex and Hydroxylapatite column. By combining IEF, SDS-PAGE and immune-blot analysis, the ethylene induced 60 kD protein was found to be an pi 6.3 peroxidase.
Abstract (Browse 1702)  |  Full Text PDF       
Application of Discriminant Analysis in Distinguishing Plant Photosynthetic Types-A Case Study in Northeast China Transect (NECT) Area
Author: TANG Hai-Ping, JIANG Gao-Ming and ZHANG Xin-Shi
Journal of Integrative Plant Biology 1999 41(10)
      
    Discriminant analysis is an important method in multivariate statistic analysis to distinguish whatever type an individual should belong to. Based on the field actual photosynthetic data obtained from the research platform--Northeast China Transect (NECT), the concept and principle of discriminant analysis were used to distinguish the different plant photosynthetic types. A number of indices related to plant photosynthetic rate measured by a LCA4 photosynthesis system were selected to build the discriminant model. In this case study, 15 plant species from C4 plant functional groups and 51 from C3 plant functional groups were selected to build a discriminant model. The rate of accuracy, of returned classification using methods of squared Mahalanobis distances from group centroids and posterior probabilities, reached to 98.48 %. With the help of this model, any plants' photosynthetic types could be distinguished simply by using their four related parameters, viz., photosynthetic rate, transpiration, stomatal conductance and the temperature difference between leaf surface and atmosphere.
Abstract (Browse 1813)  |  Full Text PDF       
Study on Age and Bunch Structure of Four Stipa Species in Inner Mongolia Plateau
Author: BAI Yong-Fei, XU Zhi-Xin, LI De-Xin and ZHAO Gang
Journal of Integrative Plant Biology 1999 41(10)
      
    The age status of four Stipa L. species, S. baicalensis Roshev., S. grandis P. Smim., S. krylovii Roshev., and S. klemenzii Roshev. in Inner Mongolia plateau was analyzed using a transect sampling method. The ontogeny of Stipa species was divided into five stages: the seedling, juvenile, reproductive, presenile, and senile. Dynamics of the population age speetra were determined mainly by the appearance of new individuals and their death rate. The survival rate of the seedlings was very low while that of file adults was high. The considerable longevity of bunch life allowed the old individuals to accumulate in the population. Both S. grandis and S. krylovii had higher proportion of seedlings than others. The proportion of the juveniles and the reproductives in the population decreased from S. baicalensis community to S. klemenzii community, but the old individuals including the preseniles and the seniles showed a reverse pattern. The aging process occurred in bunch level was characterized by the fragmentation of bunch and the within-bunch- self-thinning of the tillers. The process of within-bunch-self-thinning can be described by power equation Y: aXb. Meanwhile as a response to enviroument changes especially to the fluctuation of annual precipitation and its seasonal distribution both the numbers of reproductive shoots and vegetative shoot mass within a bunch were variable.
Abstract (Browse 1713)  |  Full Text PDF       
Species- and Habitat-variability of Photosynthesis, Transpiration and Water Use Efficiency of Different Plant Species in Maowusu Sand Area
Author: JIANG Gao-Ming and HE Wei-Ming
Journal of Integrative Plant Biology 1999 41(10)
      
    

Photosynthesis ( Pn ), transpiration (E) and water use efficiency (WUE) of more than 66 add sand species from different environmental habitats, shifting sand dune, fixed sand dune, lowland and wetland in the Maowusu Sand Area were analyzed and the relation among these characteristics and the resource utilization efficiency, taxonomic categories and growth forms of the species were assessed. The results showed that species from Chenopodiaceae, Gramineae, keguminosae which possessed the C4 photosynthesis pathway, or C3 pathway and also with nitrogen-fixation capacities had higher or the highest Pa values, i.e., 20 ¡«30μmol CO2·m£­2·s£­l, while that of evergreen shrub of Pinaceae had the lowest Pa values, i.e., 0 ¡« 5 μunol CO2·m£­2·s£­1. Those species from Compositae, Scrophuladaceae, and Gramineae with C3 pathway but no N- fixation capacity had the highest E rates, i.e., 20 ¡«30 mmol H2O· m£­2·s£­1 and again the evergreen shrub together with some species from Salicaceae and Compositae had the lowest E rates, i.e., 0 ~ 5 mmol H2O·m£­2·s£­1. Species from kegmninosae, Gramineae and Chenopodiaceae with C4 pathway or Cs pathway with N- fixation capacity, both shrubs and grasses, generally had higher WUE. However, even the physiological traits of the same species were habitat- and season-specific. The values of both Pa and E in late summer were much higher than those in early summer, with average increases of 26%, 40% respectively in the four habitats. WUE in late summer was, however, 12% lower. Generally, when the environments became drier as a result of habitats changed, i.e., in the order of wetland, lowland, fixed sand dune and shifting sand dune, Pn and E decreased but WUE increased.

Abstract (Browse 1955)  |  Full Text PDF       
Study on Enhanced Production of Taxol from Taxus chinensis var. mairei in Biphasic-liquid Culture
Author: WU Zhao-Liang, YUAN Ying-Jin, LIU Jia-Xin, XUAN Hong-Yu, HU Zong-Ding, SUN An-Ci and HU Chang-Xu
Journal of Integrative Plant Biology 1999 41(10)
      
    The effects of precursor feeding and mixed sugar supplements on the growth and taxol production of Taxus chinensis var. mairei Cheng et Fu in the biphasic-liquid culture were studied. Precursor feeding (phenylanine, benzamide and sodium acetate) and mixed sugars (maltose and sucrose) resulted in significant increase of taxol production not only in the monophasic-liquid culture but also in the biphasic-liquid culture of T. chinensis var. mairei. The synergistic function of precursor feeding on day 10 and mixed sugars on day 11 in the biphasic-liquid culture revealed more significant increase of taxol production, which was four times that of the control (the monophasic-liquid culture without precursors or mixed sugars).
Abstract (Browse 1942)  |  Full Text PDF       
Uvarilactam, a New Lactam from Uvaria microcarpa (Annonaceae)
Author: YU Dong-Lei, GUO Jian, LIAO Yong-Hong, XU Li-Zhen and YANG Shi-Lin
Journal of Integrative Plant Biology 1999 41(10)
      
    Five phenanthrene-l-carboxylic acid lactams: uvarilactam (1), aristololactam A ¢ò (2), aristololactam B ¢ñ(3), aristololactam B ¢ò (4), aristololactam A ¢ñ a (5), and two aporphine alkaloids, 4,5 dioxodehydroasimilobine (6) and oxoanolobin (7) have been isolated from the stems of Uvaria microcarpa Champ. ex Benth. Among them, 1 is a new compound and the others are isolated from this genus for the first time. Compound 3 and 4 are isolated as a mixture. Their structures were identified from interpretation of the 1H-NMR and NOESY spectral data.
Abstract (Browse 1785)  |  Full Text PDF       
Preparation of Actin and Fluorescent Actin Analogs from Plant Cells
Author: REN Hai-Yun
Journal of Integrative Plant Biology 1999 41(10)
      
    The plant actin cytoskeleton provides a dynamic cytoplasmic framework for many fundamental cellular processes like cytoplasmic streaming,cytokinesis and morphogenesis.Understanding the actin organization and structure in plants requires the generation of new probes for measuring actin dynamics in living cells. Fluorescent analog cytochemistry presents an unrivaled opportunity to probe the actin cytoskeleton in living cells. Such method using in the study of plant actin cytoskeleton has not been reported. By using this method, based on the affinity chromatography of profilin with PLP-Sepharose (PLP: poly-L-proline) for actin purification, the author obtained 6 mg of > 98% in purity, polymerizable actin from 10 g of maize (Zea mays L. ) pollen, and this actin was successfully labeled with Oregon Green 488 carboxylic acid. From 10 g of maize pollen, 1.2 mg with 60 % dye/protein ratio, polymerizable, fluorescent actin analog was obtained. The study yields an effective method for purifying plant actin and preparing fluorescent analog, which may provide facilities for the study of actin dynamics in plant ceils.
Abstract (Browse 1768)  |  Full Text PDF       
Plasma Membrane NADPH Oxidase in Tobaccoª²tobacco mosaic virus Interaction
Author: CAI Yi-Ying, ZHANG Hong-Ming and CHEN Jia
Journal of Integrative Plant Biology 1999 41(10)
      
    The plasma membrane NADPH oxidase and its regulatory role in the production of reactive oxygen species (ROS) in tobacco (Nicotiana tabacum L. )-tobacco mosaic virus (TMV) interaction was examined by using tobacco cv. "Samsun NN" (incompatible with TMV, containing the N gene for resistance to TMV) and tobacco cv. "3002" (compatible with TMV) as experimental materials. Plasma membrane (PM) vesicles were isolated from leaves of tobacco by a biphasic aqueous system. The membrane preparations were sealed, highly purified and largely in right-side-out orientation as detected by marker enzyme assays and latency studies of the PM marker, vanadate-sensitive ATPase with non-ionic detergent Triton X-100. The oxidase activity was assayed by the rate of SOD-sensitive Cyt c reduction in PM system. The oxidase activity could be increased about 80% when adding 0.01% Triton X-100 in the reactive system. This result showed that the binding-site of NADPH was on the cytosolic side of the plasma membrane and the production of O2- is on the apoplastic side. DPI (diphenylene iedonium), a specific inhibitor of the NADPH oxidase in neutrophils, also inhibited the NADPH oxidase activity in tobacco. Furthermore, the oxidase activity increased in incompatible interaction, but not in compatible interaction. The role of NADPH oxidase in the production of reactive oxygen species and stimulation of hypersensitive reaction were discussed.
Abstract (Browse 1841)  |  Full Text PDF       
Study on the Heat Shock Protein-Hpc60 from Pea Leaf Cytosol
Author: CHEN Zhong, SU Wei-Ai and TANG Zhang-Cheng
Journal of Integrative Plant Biology 1999 41(10)
      
    The effects of NaC1, PEG and exogenous ABA on the expression of the heat shock protein-Hpc60 from pea ( Pisum sativum L. ) were investigated. The results showed that PEG elevated the quantity of this heat-shock protein, much similar to heat-shock stress. Although NaC1 was able to reduce water potential at the same extent with or even higher than PEG, but it did not increase the level of this protein. This phenomenon was closely correlated with the variations of cytoplasmic ion levels. The authors postulated that NaC1 treatment caused the influx of Na + , thus disrupted the ion homeostasis, whereas PEG treatment did not. So a rather stable Na+/K+ ratio was sustained in the plant cell treated with PEG, which might be prerequisite for the induction of Hpc60. In addition, exogenous ABA was employed in the authors' experiment which showed no influence on the synthesis of this heat-shock protein.
Abstract (Browse 1796)  |  Full Text PDF       
Reactivation of Partially Metalloclusterª²deficient MoFe Protein by Rhenium-containing Reconstituent Solution
Author: HUANG Ju-Fu, WANG Zhi-Ping, WANG Dao-Yong, DONG Zhi-Gang and LI Jia-Ge
Journal of Integrative Plant Biology 1999 41(10)
      
    When the reduced MoFe protein from Azotobacter vinelandii Lipmann was treated with ophenanthroline and air, an inactive protein partially deficient in both FeMoco and P-cluster could be obtained. After incubating the treated protein with a reconstituent solution containing Re2OT, ferric homocitrate, Na2S and dithiothreitol, which had no circular dichroism (CD) signal, the ultraviolet and visible CD spectra, the C2H2 and H+ -reduction activity of the incubated protein were significantly recovered. However, the spectra were somewhat different from those of the reduced MoFe protein. The results showed that: 1) in the incubated protein solution there was possibly a new recombined ReFe protein besides the intact MoFe protein which was not destroyed by the treatment with o-phenanthroline and air; 2) it might be possible that both ReFe protein and MoFe protein exhibited similar ability of nitrogen fixation, although they were somewhat different in structure.
Abstract (Browse 1664)  |  Full Text PDF       
RAPD Study on Interª²species Relationships in Roegneria (Poaceae: Triticeae)
Author: ZHOU Yong-Hong, YANG Jun-Liang, ZHENG You-Liang, YAN Ji, JIA Ji-Zeng and WEI Yu-Ming
Journal of Integrative Plant Biology 1999 41(10)
      
    To assess the relationships among 26 species in Roegneria C. Koch, 34 random decamer primers were screened for RAPD fragments. 28 primers produced polymorphic RAPD products. Data from 16 primers were used for RAPD assay. By NTSYS-pc program, Jaccard' s genetic similarity coefficients were generated and dendrogram was constructed using UPGMA. It is concluded as follows: (1) Distinct genetic differences and extensive genetic diversity were present among the species. (2) There were some genetic differences between StY and StYH genomes, and StY and StYH had a certain degree of differentiations respectively which were related to geographic regions, the farther the geographic distribution between species, the less the similarity to each other. (3) When different accessions in a species, such as species with similar morphological characters, homologous genomes and similar geographic distribution, were clustered together respectively, it suggusted that they had closer relationships. (4) The awnless species R. alashanica Keng and R. magnicaespes (D. F. Cui) L. B. Cai, in Roegneria, were separated from the other species analysed in this study, indicating that these two species had intensive genetic differences from the others. (5) R. caucasica C. Koch, a species from Western Asia, was quite different from the other species contained StY genomes in Roegneria from Eastern Asia and Central Asia. (6) The results were in consistance with that of the analysis of morphology and chromosome pairing in the taxonomic treatments for R. ciliaris (Trin) Nevski and R. japonensis (Honda) Keng, R. tenuispica J. L. Yang et Y. H. Zhou and R. pendulina Nevski, and R. tsukushiensis (Honda) Ohwi and R. kamoji Ohwi. The present study discussed the usefulness of RAPD markers in the systematic study of Roegneria.
Abstract (Browse 1858)  |  Full Text PDF       
Change of Endogenous IAA During Cambial Activity and IAA-binding Protein in Cambial Cells in Broussonetia papyrifera
Author: CUI Ke-Ming, WANG Xiang-Bin, DUAN Jun-Hua and WANG Su-Yun
Journal of Integrative Plant Biology 1999 41(10)
      
    Change in the concentration of endogenous IAA was measured with enzyme-linked immtmosorbent assay (ELISA), and the distribution of IAA-binding protein in the protoplasts in the cambial region was tested with an immuno-fluorescence technique, being improved by the authors, during the cambial active period in Broussonetia papyrifera (L.) Vent. The results showed that the concentration of the endogenous IAA increased rapidly as the immature xylem and phloem were luxuriantly formed from the cambium, and decreased and maintained at a certain lower level when the immature vascular cells differentiated toward maturation. The IAA- binding protein was mainly distributed in the plasmalemma, protoplasm, nuclear envelope and nuclear substance.
Abstract (Browse 1708)  |  Full Text PDF       
Studies on Highly Efficient Planting of Transgenic Cotton
Author: WANG Wei, CHEN Wan-Xin, ZHU Zhen, XU Hong-Lin, GAO Yue-Feng, WU Qian, ZHU Yu, GUO Zhong-Chen, LI Xiang-Hui
Journal of Integrative Plant Biology 1999 41(10)
      
    Foreign insecticidal genes were transferred into 4 upland cotton ( Gossypium hirsutum L. ) cultivars by Agrobacterium-mediated transformation. It was found that planting style has a critical influence on the survival rate of the transgenic plantlets. Direct transplanting, culturing in liquid medium or vermiculite before transplanting were not proper for planting transgenic plantlets. The survival rate was quite low and hardening time was rather long. Graft could greatly enhance the survival rate and efficiently reduced the hardening time, thus paves the way for cotton improvements through genetic engineering and other biotechnology.
Abstract (Browse 1710)  |  Full Text PDF       
A Non segregation F2 Population Derived from the Cross of Triploid¡ÁDiploid in Rice
Author: WU Xian-Jun, WANG Xu-Dong, ZHOU Kai-Da and ZHU Li-Huang
Journal of Integrative Plant Biology 1999 41(10)
      
    A non-segregating F2 population was obtained from the cross of tfiploid ¡Á diploid in rice ( Oryza sativa L. ). The maternal parent used in cross was SAR-3 which is an autotriploid occurring in the field, and the paternal was a restored line Sheng 47. The F2 population from the same F1 plant appears uniform, and so does F3. Results from coefficients of variation (CV) of F2, Ducan¡¯s test of F3 and SSRs marker analysis showed that the F2 of SAR-3 ¡Á Sheng 47 was non-segregating. Markers for either parent could be present in F2 progenies. In some cases, both markers for each parent could be found on one chromosome. The authors presume that after crossing there might be recombination of chromosome followed by homozygotization. Stability of early generation could be utilized in breeding, as it is a new approach to shorten the breeding procedures.
Abstract (Browse 1807)  |  Full Text PDF       
Genetic Variation of Main Parents of Hybrid Rice in China Was Revealed with Simple Sequence Repeat Markers
Author: LI Yun-Hai,XIAO Han, ZHANG Chun-Qing, HU Guo-Cheng, YU Yong-Hong, JIA Ji-Zeng and SUN Zong-Xiu
Journal of Integrative Plant Biology 1999 41(10)
      
    Total thirty throe parents of hybrid rice ( Oryza sativa L. ), including twenty five rice male sterile lines, throe maintainer lines and five restore lines, were analyzed by using twenty SSR (simple sequence repeats) primer pairs which disperse on 12 chromosomes in rice. Those primers detected 102 alleles among 33 parents of hybrid rice. PIC (polymorphic index content) values ranged from 0.274 to 0.773. PIC value is 0.554 on the average. Five SSR primer pairs selected from fifty six primer pairs can distinguish individually all of the rice male sterile lines and restore lines in the present study. As results from cluster analysis, it was concluded as follows. (1) Rice male sterile lines have abundant genetic diversity in China. Genetic background was vulnerable among rice male sterile lines used in large scale. (2) In indica rice, genetic variation of the restored lines is larger than that of the male sterile lines. (3) The restored lines were clustered separately from the male sterile lines which are grown widely for production.
Abstract (Browse 1820)  |  Full Text PDF       
Transmission of 6VS Chromosome in Wheat- Haynaldia villosa Translocation Lines and Genetic Stability of Pm21 Carried by 6VS
Author: LIU Jin-Yuan, TAO Wen-Jing, LIU Da-Jun, CHEN Pei-Du, LI Wan-Long, XIANG Qi-Jun and DUAN Xia-Yu
Journal of Integrative Plant Biology 1999 41(10)
      
    Fa plants from Yang94-138 ¡Á92R149 were analyzed using species-specific probe pHv62 for Haynaldia villosa (L.) Schur and RFLP probe Psrll3 located on the short ann of homoeologous group six for wheat ( Triticum aestivum L. ). The results showed that the transmission rate of 6VS in the F2 was 69.5 %, which was close to the expected value of 75%. 147 F2 plants from the above Fl plant were tested for their resistance in seedling stage to 6 pathogenic strains of powdery mildew. It was observed that Pm21 genes on 6VS inherited dominantly and expressed well when transferred to wheat£­"Yangmai 158" genotype.
Abstract (Browse 1711)  |  Full Text PDF       
Expression Analysis of Polyubiquitin Genes from Bean in Response to Heavy Metals
Author: CHAI Tuan-Yao and ZHANG Yu-Xiu
Journal of Integrative Plant Biology 1999 41(10)
      
    Using differential screening of a leaf eDNA library prepared from a bean cultivar (Phaseolus vulgaris L. tv. Saxa) exposed to HgCl2, the authors have isolated and characterized two heavy metal-regulated eDNA fragments, designated as PvSR5 and PvSRS1 (Phaseolus vulgaris stress-related gene). The sequences of the eDNA inserts and homological analysis showed that both PvSR5 and PvSR51 encode a polyubiquitin respectively. The polyubiquitin genes were constitutively expressed in roots but weakly expressed in stems and leaves. Northern blot analysis revealed a low level of transcripts of polyubiquitin in unstressed bean leaves, but the 'gene expression was strongly stimulated by heavy metals, elevated temperature and salicylic acid, whereas wounding had almost no effect. These suggested that polyubiquitin might play important roles in resistance to heavy metals and various environmental stresses.
Abstract (Browse 1663)  |  Full Text PDF       
Studies on Transfer of Bt Gene into Glycine max
Author: SU Yan-Hui, WANG Hui-Li, YU Mei-Min, L¨· De-Yang and GUO San-Dui
Journal of Integrative Plant Biology 1999 41(10)
      
    Transgenic plants were regenerated from soybean ( Glycine max (L.) Merr. ) immature cotyledons bombarded by gene-gun with plasmid pGB14AB which contained Bacillus thuringiensis Berliner insecticidal crystal protein gene (Bt gene )and GUS gene, and from cotyledon nodes infected with LBA4404 which contained pGB14AB. Immature cotyledons of "Zhonghuang No. 4' (variety) and 8502 (line) differentiated many more somatic embryos than others. Desiccation treatment of somatic embryos from immature cotyledons of "Zhonghuang No. 4" improved significantly their germination frequency. Preculturing of immature cotyledon was beneficial for somatic embryogenesis from the cotyledons infected with Agrobacterium tumefaciens (Smith et Townsend) Conn. High efficiency of transformation is related to selection of genotype and recipient, desiccation treatment of somatic embryos and improvement of transformed system in soybean.
Abstract (Browse 1716)  |  Full Text PDF       
Molecular Cloning of GAFP-1, an Antifungal Protein from Gastrodia elata
Author: WANG Xiao-Chen, Willson Ardiles DIAZ, Guy BAUW, XU Qing, Marc Wan MONTAGU, CHEN Zhang-Liang, Willy DILLEN
Journal of Integrative Plant Biology 1999 41(10)
      
    The cloning and sequencing of a full length cDNA of GAFP-1 ( Gastrodia antifungal protein), an antifungal protein from Gastrodia elata BI. f. fiavida S. Chow is reported. Degenerate primers were designed based on the N-terminal partial sequence from purified GAFP-1 to amplify the corresponding cDNA by rapid amplification of cDNA ends (RACE). A cDNA was obtained that contains an open reading frame for a peptide of 171 amino acids which matches the known peptide sequences. A 5'UTR (untranslated region) of 55 bp was found upstream from the translation initiation site. Two poly(A) adenylation sites were located downstream the stop codon. GAFP-1 cDNA and its deduced amino acid sequence share high homology with the mannose binding lectins from Epipactis helloborine, Listera ovata and snowdrop ( Galanthus nivalis ). The cDNA can now be used for testing the potential of GAFP-1 for engineering fungal resistance in crop plants.
Abstract (Browse 1890)  |  Full Text PDF       
Cloning and Expression of a New Gene Encoding an Antifungal Protein in Phytolacca americana
Author: LIU Ying-Fang, WANG Chun-Xia, ZHAO Jin-Dong and WU Guang-Yao
Journal of Integrative Plant Biology 1999 41(10)
      
    protein (Pa-AFP) with molecular weight about 4 kD was purified from the seeds of Phytolacca americana L. , which obviously inhibits the growth of Rhizoctonia solani Kiihn in vitro. The authors isolated mRNA from the seeds of pokeberry and designed a degenerate PCR primer according to the N-terminal sequence of the purified protein. The full-length cDNA encoding Pa-AFP was cloned by RT-PCR and 5'-RACE and sequenced. The deduced amino acid sequence indicates that a preprotein with 65 amino acid residues is firstly translated and then processed to a mature protein with 38 amino acids. The DNA encoding the mature protein was subcloned into expression vector pGEX-4T1, and expressed efficiently in E. coli BL21 as a GST- Pa-AFP fusion protein. The fusion protein was purified by glutathione-Sepharose 4B affinity colmnn chromatography. The purified fusion protein was specifically digested by thrombin and the Pa-AFP was further purified by filtration column chromatography.
Abstract (Browse 1743)  |  Full Text PDF       
The Role of Calcium in the Fertilization Process in Flowering Plants
Author: YANG Hong-Yuan
Journal of Integrative Plant Biology 1999 41(10)
      
    The fertilization process in flowering plants in a broad sense includes a progamic phase preceding the phase of double fertilization. To our present knowledge, calcium as a second messenger in the signal transduction plays important roles in all the links of this process. The present review attempts to highlight the recent advances in this research field, including: calcium in relation to in vitro pollen tube growth (distribution of calcium in pollen tube tip; regulation of pollen tube growth by calcium; calcium oscillation in pollen tube); distribution of calcium in pistil and its relation to in vivo pollen tube growth (calcium in relation to pollen-pistil recognition; pollen tube growth in pistil; pollen tube entry into embryo sac and the discharge and transportation of sperms); and calcium in relation to sperm-egg fusion and egg cell activation. In conclusion the author summarizes into several main view points, and gives recommendation for further researches on this topic.
Abstract (Browse 2173)  |  Full Text PDF       
 

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