October 2002, Volume 44 Issue 10

 

          Research Articles
A Comparative Study on Detection of the Expression of Alternative Oxidase in Tobacco Callus with the Monoclonal Antibody Against Alternative Oxidase and Antibody Against Synthetic Polypeptide Antibody
Author: YAN Ying Cai, LIN Hong Hui*, LIANG Hou Guo, DU Lin Fang
Journal of Integrative Plant Biology 2002 44(10): 1255-1257
      
    

单克隆抗体和抗合成多肽抗体检测烟草愈伤组织中交替氧化酶表达的比较
晏婴才 林宏辉*  梁厚果 杜林方
(四川大学生命科学学院,成都610064I)


摘要: 从经过不同温度处理的烟草(Nicotiana rustica L.)愈伤组织中提取并纯化线粒体蛋白,分别与交替氧化酶的单克隆抗体和抗合成多肽抗体进行免疫杂交。结果表明:交替氧化酶的含量随温度的下降而显著上升;单克隆抗体的特异性较高于抗合成多肽抗体,但后者与交替氧化酶同样有良好的亲和性。因此,用合成多肽方法制备的抗体可以用于交替氧化酶的研究中。
关键词: 交替氧化酶;单克隆抗体;抗合成多肽抗体;免疫杂交;烟草愈伤组织

Abstract (Browse 1989)  |  Full Text PDF       
Chromosome Location of the Male-sterility and Yellow Seedling Gene in Line 1066A of Foxtail Millet
Author: WANG Run-Qi, GAO Jun-Hua, MAO Li-Ping, DU Rui-Heng, DIAO Xian-Min and SUN Jing-San
Journal of Integrative Plant Biology 2002 44(10): 1209-1212
      
    

Using foxtail millet (Setaria italica(L.) Beauv.) male sterile line 1066A as female parent and Yugu 1 primary trisomic series (1-7) and tetrasomics 8, 9 as male parents, chromosome location of gene for male sterility and yellow seedling in line 1066A was studied by primary trisomic analysis. The plants of F1 generation of trisomics 2-9 were obtained by crossing with a great many plants of 1066A. F1 generation of trisomics was similar to theirmale parent in morphologic characters, the color of their seedling was green, and pollen was partially fertile. The segregation ratio of fertility to sterility is 3∶1 in F2 generation of trisomics 2, 3, 4, 5, 7, 8 and 9; and 14∶1 only in F2 generation of trisomic 6 (χ20.05=0.012). The segregation ratio of green seedling to yellow seedling is 12∶1 only in F2 generation of trisomic 7 (χ20.05=0.31), but in other cases, this ratio is 3∶1. The results indicated that the male sterility gene was located on chromosome 6, and the gene for yellow seedling was monogenic recessive and located on chromosome 7. The rate of trisomics transmission by pollen was tested, trisomics 8 and 9 were the highest in rates of trisomics transmission and followed by trisomics 6 and 4.

谷子雄性不育系1066A 不育基因和黄苗基因的染色体定位
王润奇1* 高俊华1! 毛丽萍2杜瑞恒1刁现民1*孙敬三3

(1. 河北省农林科学院谷子研究所,石家庄050031;2. 石家庄市桥东污水治理工程筹建处,石家庄050031;3. 中国科学院植物研究所,北京100093)

摘要: 以谷子 (Setariaitalica (L .)Beauv .)雄性不育系 10 6 6A为母本 ,豫谷 1号三体 (1~ 7)及四体 8和四体 9作父本进行杂交 ,应用初级三体分析法 ,进行了谷子雄性不育基因和黄苗基因的染色体定位研究。通过配置大量杂交组合和反复授粉 ,利用豫谷 1号三体的极少量花粉 ,获得了三体 2~ 9的F1代杂种 ,各杂种三体的形态与豫谷 1号三体基本相似 ,略有差异 ,苗色呈绿色且可育。杂种F2 植株的苗色和育性都产生分离。结果是三体 3、5、7、8、9的F2 代分离出的可育株与不育株之比为 3∶1,三体 6的可育株与不育株之比为 14∶1(χ2 =0 .0 12 ,P =0 .0 1)。杂种F2分离出的绿苗与黄苗之比只有三体 7为 12∶1(χ2 =0 .36 ,P =0 .0 1) ,其他均为 3∶1。因此 ,可以确定 10 6 6A的不育基因为隐性单基因 ,位于第 6号染色体上 ,该品系的黄苗基因也是隐性单基因 ,位于第 7号染色体上。

关键词: 谷子;雄性不育;黄苗;三体分析法;基因定位

Abstract (Browse 2353)  |  Full Text PDF       
Cell Growth and Ajmalicine Accumulation in a Full Habituated Catharanthus roseusCell Line C20hi
Author: ZHENG Zhen Gui*, ZHOU Yu, LIU Di, HU Zhi Bi
Journal of Integrative Plant Biology 2002 44(10): 1146-1150
      
    

A full habituated cell line C20 hi was screened from 2,4 D dependent line (C20D) of Catharanthus roseus (L.) G. Don. The investigation involved the cell growth, ajmalicine production and enzyme activity related to indole alkaloid biosynthesisin both cell lines. These results indicated that C20 hi cells grew faster than C20D cells, and average ajmalicine content in C20 hi cells was 18.4 times more than that in C20D when cultured in the production medium. In the growth medium, average aj malicine content in C20hi cells was 31.9 times more than that in C20D cells, while the cell growth has no obvious difference. The comparison of enzyme activities in C20hi and C20D cells indicated that tryptophan decarboxylase (TDC),strictosidine synthase (SSS) and geraniol 10 dehydrogenase (G10H) activities have no close relation to ajmalicine accumulation, although the activities of these enzymes were higher when cells were cultured in the production medium than in the growth medium. The C20hi cells are relatively stable in five years of culture.

长春花激素完全适应型细胞的生长和阿玛碱合成特性
郑珍贵* 周 煜 刘 涤 胡之璧
(上海中医药大学中药研究所,上海200032)

摘要: 从长春花激素依赖型细胞系 (C2 0D)筛选出一种激素完全适应型的细胞系 (C2 0 hi) ,考察了两种细胞生长、阿玛碱合成和与吲哚生物碱生物合成相关的酶的活性 ,结果表明 :在生长培养基上二者生长无显著差异 ,而C2 0 hi细胞平均阿玛碱含量是C2 0 D的 31 9倍 ,在生产培养基上C2 0 hi细胞生长较C2 0 D快 ,C2 0 hi平均阿玛碱含量是C2 0 D的1 8 4倍。通过比较生产和生长培养基中C2 0 hi细胞的色氨酸脱羧酶、异胡豆苷合酶和牛儿醇-1 0-脱氢酶活性 ,发现在生产培养基中培养细胞的 3种酶活性均显著高于生长培养基 ,但与阿玛碱积累没有密切关系。研究结果还表明 ,通过 5年的继代培养 ,激素完全适应型细胞系C2 0 hi的阿玛碱含量是比较稳定的。
关键词: 完全适应型细胞;阿玛碱;细胞培养;长春花

通讯作者。Tel.:021-54231633;Fax:021-64177325;E-mail:<zhengzhegnui @ 163.com>

Abstract (Browse 2102)  |  Full Text PDF       
Water Relations on Alhagi sparsifolia in the Southern Fringe of Taklamakan Desert
Author: LI Xiang-Yi, ZHANG Xi-Ming, ZENG Fan-Jiang, Andrea FOETZKI, Frank M. THOMAS, LI Xiao-Ming, Michael RUNGE and HE Xing-Yuan
Journal of Integrative Plant Biology 2002 44(10): 1219-1224
      
    

Water relations of Alhagi sparsifolia Shap. at the transition zone between oases and sandy desert were studied in the southern fringe of Taklamakan Desert. Results showed that A. sparsifolia maintained the positive turgor during the summer. The steady high predawn water potential (ψP) indicated A. sparsifolia had sufficient hydration and water supply in growing season. In July, water deficitcaused by drought stress had no effect on the transpiration of A. sparsifolia. Therefore, drought stress is not a main factor affecting the survival of plants. The physiological adaptation to drought of A. sparsifolia was shown mainlyat the leaf level by significant difference (ΔΠ) and relative water content (RWC)between the osmotic pressure at full turgor and at turgor loss, by occurring of osmotic adjustment, by high percentage of dry mass related water content (WC sat), by RWC at turgor loss point (RWCp) in stable level and low RWC of the saturated symplast (RWCsym). However, the morphological features of transpiring surface reduction and deep root system seem to be the main way for the plant to adapt to the extreme drought environment. Result also suggests that one time of irregular irrigation in summer will not be helpful to recover water status of A. sparsifolia in location where the water table is very low. 

塔干南缘骆驼刺植被水分关系的研究
李向义1,2* 张希明1曾凡江1 Andrea  FOETZKI3 Frank M.THOMAS3 李小明1  Michael RUNCE3  何兴元2

(1. 中国科学院新疆生态与地理研究所,乌鲁木齐830011;2. 中国科学院沈阳应用生态研究所,沈阳110016;3 . 德国哥廷根大学生态与生态系统研究所,哥廷根d-37073)

摘要: 对塔克拉玛干沙漠———绿洲过渡带骆驼刺 (Alhagisparsifolia Shap .)水分关系的研究表明 :骆驼刺在夏季保持了正的膨压 ,一直较高较稳定的清晨水势说明植物水分恢复状况良好 ,植物得到了较好的水分供应 ;在 7月 ,干旱胁迫造成的水分亏缺并未影响植株正常的蒸腾作用 ,因而干旱引起的水分胁迫并未威胁到植被的存在。骆驼刺对干旱胁迫的水分生理适应主要体现在叶水平上 ,表现为饱和枝条的渗透势 (IIo)和膨压消失点的渗透势 (II p)的差值 (Δ II)和相对含水量 (RWC)在膨压消失点间更大的变化、渗透调节的产生、较高较稳定的饱和枝条水分与干物质之比 (WCsat)和膨压消失点的相对含水量 (RWC p) ,以及较低的共质体水在总水分中的相对含量 (RWCsym)。但形态学上的特征 ,主要表现为深而发达的根系和蒸腾面积的减少 ,才是骆驼刺适应极端干旱环境的主要途径。非定期的夏季一次性灌溉对地下水位很低地区的骆驼刺植被水分状况的恢复没有帮助。

关键词: 骆驼刺;水分关系;干旱胁迫;水势;EF 参数;灌溉效果

通讯作者。 Tel.:0991-3842425; E-mail: <xiangyil @ yahoo.com>

Abstract (Browse 2329)  |  Full Text PDF       
The Fusion Patterns During Sexual Cell Fusion
Author: SUN Meng Xiang*, YANG Hong Yuan, Mauro CRESTI
Journal of Integrative Plant Biology 2002 44(10): 1142-1145
      
    

Large scale of sexual cell fusion was carried out in order to observe the fusion pattern during in vitro fertilization with special interest in the influence of cell volume on the membrane behavior. Three patterns could be recognized in sexual cell fusion, which was supported by fluorescent microscopy with the aid of video enhanced system and cooled CCD. It was found that the fusion pattern and cell membrane behavior were related to the cell volume ratio of two fusing cells. The results reported here might be useful for interpreting and evaluating data from in vitro fertilization experiments and for explaining male cytoplasmexclusion during fertilization.

体外受精研究:玉米和烟草性细胞融合的几种模式
孙蒙祥1* , 杨弘远1, Mauro VRESTI2
(1.  武汉大学植物发育生物学教育部重点实验室,武汉430072;2.Department of Environmental Science, Siena Unixersity,Siena 53100,Utaly)

摘要: 分别用玉米(Zea mays  L.)和烟草(Nicotiana tabacum L.)为材料,以单对细胞融合技术广泛开展了各种组合的性细胞的融合实验.重点观察了性细胞融合过程中细胞体积对细胞膜融合行为的影响.视频增差显微术结合细胞膜荧光标记技术显示,性细胞融合过程中受融合双方体积差异大小的调节,细胞膜的融合行为可归纳为3种基本模式发现在体外受精条件下精细胞可以类似被吞噬的方式进入卵细胞,且其融合动作非常迅速.由此提出一种假设机制用以解释体内受精过程中雄性细胞质被排除的可能原因.
 

关键词: 体外受精;玉米;烟草;细胞体积;融合模式;FITC-Con A

通讯作者。E-mail: <mxsun @ whu.edu.cn>.

Abstract (Browse 2027)  |  Full Text PDF       
Elicitation on Artemisinin Biosynthesis in Artemisia annua Hairy Roots by the Oligosaccharide Extract from the Endophytic Colletotrichumsp. B501
Author: WANG Jian Wen, XIA Zhong Hao, TAN Ren Xiang*
Journal of Integrative Plant Biology 2002 44(10): 1233-1238
      
    

The oligosaccharide elicitor from the mycelial wall of an endophytic Colletotrichum sp. B501 promoted the production of artemisinin in Artemisia annua L. hairy root culture. When hairy roots of 22 day old cultures (later growth phase) were exposed to the elicitor (20 mg/L) for 4 d, the maximum content of artemisinin reached 1.15 mg/g, a 64.29% increment over the control. The electron X ray microanalysis disclosed the rapid accumulation of Ca2+ in the elicited cortical cells of hairy root. The electronic microscope observation revealed the high electron density area in vacuole of elicited cells. During the first day of elicitation the peroxidase activity of hairy roots was improved sharply. Some cellular morphological changes including cell shrinkage, condensation of cytoplasm and nuclear fragmentation, coincident with the appearance of DNA ladders, were observed after the third day of elicitation. It was suggested that the oligosacc haride elicitor triggered the programmed cell death, which may provide the subst ance or chemical signal for artemisinin biosynthesis.

内生真菌 Colletotrichum sp. B501的寡糖提取物对黄花蒿发根中青蒿素生物合成的诱导
王剑文 夏仲豪 谭仁祥*

(南京大学功能生物分子研究所,南京210093)

在黄花蒿 (Artemisiaannua L .)发根液体培养中 ,黄花蒿内生炭疽菌 (Colletotrichum sp .B5 0 1)细胞壁寡糖提取物可促进发根青蒿素的合成。经寡糖诱导子 (2 0mg/L)处理 4d后 ,发根青蒿素含量达 1.15mg/g ,比对照高出6 4 .2 9%。诱导作用与诱导子浓度、作用时间相关。诱导处理 1d后 ,X射线能谱分析表明黄花蒿发根细胞中Ca2 + 积累量显著增高 ,电镜观察发现液泡内出现高电子致密物 ,具活性氧清除作用的过氧化物酶表现出高活性 (6 .5unit·min-1·g-1FW)。诱导处理第三天 ,细胞核DNA呈梯度条带降解 ,部分细胞出现程序化死亡。内生菌细胞壁寡糖提取物引起的生理反应有利于细胞中青蒿素的生物合成。
 

关键词: 黄花蒿;内生真菌;寡糖诱导子;青蒿素;诱导反应

 

Abstract (Browse 2850)  |  Full Text PDF       
Cloning and Expression Patterns of a Metallothionein like Gene htMT2 of Helianthus tuberosus
Author: CHANG Tuan Jie, CHEN Lei, LU Zi Xian, CHEN Wan Xin, LIU Xiang, ZHU Zhen*
Journal of Integrative Plant Biology 2002 44(10): 1188-1193
      
    

A novel cDNA sequence htMT2, which encodes a type 2 metallothionein like protein, was isolated from Helianthus tuberosus L. tuber cDNA library. The whole sequence is 509 bp, including an open reading frame (ORF) of 240 bp, a 5′UTR of 62 bp and a 3′UTR of 207 bp. Two genomic sequences covering the coding region of htMT2 were cloned by PCR reaction. Sequence analysis revealed that the genomic sequences htMTG-1 of 986 bp and htMTG-2 of 982 bp were both composed of three exons and two introns. The deduced protein consisted of 79 amino acid residues with a predicted molecular weight of 7.8 ku (kD). Amino terminal and carboxy terminal domains contained 8 and 7 cysteine residues respectively, separated by a central cysteine free spacer. Sequence alignment revealed that the predicted protein of htMT2 was homologous to type 2 metallothioneins (MTs) of plants. Southern blotting analysis indicated that htMT2 was encoded by a small multi gene family in H. tuberosus genome. Northern blotting analysis showed that htMT2 transcripts were detected in stems, leaves and leafstalks, but no transcripts were detected in roots. The expression level in stems was the highest among the above tissues. Transcripts in stems were significantly reduced by Cu2+ treatment. Judging from the homologies between the deduced HtMT2 and other type 2 plant metallothioneins as well as responses to metal ions, we believe that htMT2 encodes a new type 2 metallothionein.

菊芋类金属硫蛋白基因htMT2的克隆及其表达特征分析
常团结 陈 蕾 路子显 陈宛新 刘 翔 朱 祯*

(中国科学院遗传与发育生物学研究所,北京100101)

摘要: 从菊芋 (Helianthustuberosus L .)块茎cDNA文库中得到了一个新的植物类金属硫蛋白基因htMT2的cDNA序列 ,全长 5 0 9bp ,包括 2 4 0bp的开放阅读框、6 2bp的 5′端非翻译区、2 0 7bp的 3′端非翻译区。通过PCR获得了 2个htMT2编码区的部分基因组片段htMTG_1及htMTG_2 ,长度分别为 986bp和 982bp。分析表明两个基因组片段均包含3个外显子及 2个内含子 ,编码一个由 79个氨基酸残基组成的多肽 ,与从htMT2推测的多肽完全一致 ,该多肽具有植物类金属硫蛋白的典型结构特征 ,N端及C端结构域富含Cys ,分别具有 8个和 7个Cys残基 ,上述两个结构域被一个无Cys的中间区分开。Southern杂交结果表明 ,htMT2在菊芋基因组中以小基因家族的形式存在。Northern杂交结果表明htMT2在叶片、叶柄、茎及块茎中均有表达 ,在茎中有较高水平的表达 ,但在根中未检测到杂交信号。经Cu2 + 处理后 ,htMT2在茎中的表达量显著降低。与其他 2型金属硫蛋白的序列同源性比较及htMT2对金属离子处理的反应均表明 ,htMT2是一种新的植物类金属硫蛋白基因。
关键词: 植物类金属硫蛋白;;RB9 序列;基因表达;金属离子处理;内含子

通讯作者。电话:010-64873490;E-mail:<zhou @ genetics.ac.cn>.

Abstract (Browse 2283)  |  Full Text PDF       
Cloning and Characterization of the Na+/H+ Antiport Genes from Triticum aestivum
Author: WANG Zi Ning, ZHANG Jin Song, GUO Bei Hai, HE Si Jie, TIAN Ai Guo, CHEN Shou Yi
Journal of Integrative Plant Biology 2002 44(10): 1203-1208
      
    

The Na+/H+ antiport genes named TaNHX1 and TaNHX2 were cloned by screening a salt stressed wheat cDNA library using rice Na+/H+ antiport cDNA fragment as the probe. Sequencing analysis showed that TaNHX1 was 2 029 bp in length and contained a complete ORF of 1 638 bp. The TaNHX1 encodes a polypeptide of 546 amino acids with a transmembrane domain DIFFIYLLPPI. TaNHX2 was 1 693 bp in length consisting of a partial ORF followed by a 3′ UTR of 808 bp. The amino acid sequence of these two genes were about 70% identical to the known NHX genes from rice, Arabidopsis and Atriplex. ART PCR assay showed that the level of TaNHX1 transcripts was increased and reached a steady higher level in the seedlings after 3 h treatment with 400 mmol/L NaCl.

小麦Na+/h+反转运蛋白基因的克隆和特性
王子宁1,2 张劲松1 郭北海2 何锶洁1 田爱国1 陈受宜1*

(1. 中国科学院遗传研究所植物生物技术重点实验室,北京100101;2. 河北农林科学院粮油作物研究所作物育种室,石家庄050031)

摘要: 以水稻(Oryza sativa L.) Na+/H+反转运蛋白cDNA片段为探针,从小麦盐胁迫cDNA文库中筛选和克隆了2个小麦Na+/H+反转运蛋白基因,分别命名为TaNHX1 TaNHX2.序列分析表明TaNHX1为2 029 bp,包含一个完整的1 638 bp的ORF,编码546个氨基酸,其中含有DIFFIYLLPPI跨膜区.TaNHX2为1 693 bp,包含部分ORF及808 bp的3′-UTR.这2个基因与已知的水稻、拟南芥(Arabidopsis thialiana)和滨藜(Atriplex gmelini)中的同类基因NHX的相似性约为70%.RT-PCR分析表明小麦苗经400 mmol/L NaCl处理1 h后,TaNHX1的转录水平有所提高.

关键词: 小麦;Na+/H+ 反转运蛋白基因;盐诱导

Abstract (Browse 2191)  |  Full Text PDF       
Chemical Constituents of Epimedium platyetalum
Author: WANG Jun-Xian, FAN Xiao-Wen, LU Xiu-Mei, NIU Juan-Fang and ZHU Rong
Journal of Integrative Plant Biology 2002 44(10): 1258-1260
      
    

茂汶淫羊藿化学成分的研究
王军宪* 范晓雯 吕修梅 牛娟芳 朱 蓉
(西安交通大学药学院,西安710061)

摘要: 从小檗科植物茂汶淫羊藿 (Epimediumplatyetalum K .Meyer)的地上部分首次分离得到 5种化合物 (1- 5 ) ,经化学和波谱法 (UV、IR、NMR和FAB-MS)鉴定 ,其中两种为新化合物 ,命名为茂藿甙A (3,5-dihydroxy-4′-methoxy-8-(3″-hydroxyisopentyl)_flavone-7-O-β-D-glucopyranoside ,1)和茂藿甙B (5-hydroxy-4′-methoxy-8-prenyl-flavone-3-O-β-D-glu copyranosyl(1→ 4 -O-α-L-rhamnopyranosyl-7-O-β-D-glucopyranoside ,2 ) ,另三种为淫羊藿甙 (3)、宝藿甙-Ⅰ (4)和胡萝卜甙 (5 )。

关键词: 小檗科;茂汶淫羊藿;茂藿甙A;茂藿甙B

通讯作者。E-mail: <wngjx @ xjtu.edu.cn>.

Abstract (Browse 2223)  |  Full Text PDF       
The Occurrence of Longfengshania in the Early Cambrian from Haikou, Yunnan, China
Author: XU Zhao Liang 
Journal of Integrative Plant Biology 2002 44(10): 1250-1254
      
    

Well preserved Early Cambrian small individual megascopic carbonaceous algal fossils have been found from the Chengjiang Biota. This paper deals with the new megascopic algal fossils in the Early Cambrian also from Chengjiang Biota at Ercai Village, Haikou, Kunming, Yunnan, southwest China. Two new taxa,Longfengshania cordata sp. nov. and Plantulaformis sinensis gen. et sp. nov. are described here in detail. These new findings show that the Chengjiang Biota is high in species diversity of fossil algae and might offer new evidence for a better understanding of the Cambrian explosive biological evolution. Moreover, the fossils described here might belong to the megaalgal fossils according to detailed morphological study on the relationships of Longfengshania. Additionally, the occurrence of Longfengshania in the early Cambrian and the Neoproterozoic probably indicates a wide ecological tolerance and flexibility and apparently reflects its evolutionary conservation and a high degree of inherent genetic stability.

龙凤山藻在澄江早寒武世生物群中的发现
徐兆良
(中国科学院植物研究所,北京100093)

摘要: 主要报道了产自云南昆明海口耳材村早寒武世筇竹寺组玉案山段澄江生物群中可能为自由漂浮生长的宏观藻类化石———心型龙凤山藻 (新种 ) (Longfengshaniacordata sp .nov .)和中华豆芽藻 (新属、新种 ) (Plantulaformissinensis gen .etsp .nov .)。这些化石的发现进一步显示了澄江生物群物种多样性 ,为揭示该生物群爆发性演化提供了新的证据。通过对龙凤山藻属亲缘关系的深入比较研究 ,进一步证明该类化石应归属宏观藻类以及它们遗传上的稳定性、演化上的保守性和环境上的适应性。
 

关键词: 昆明海口;早寒武世;藻类化石

Abstract (Browse 2073)  |  Full Text PDF       
Tempo Spatial Variations in Stomatal Conductance, Aperture and Density of Ligustrum sinense Acclimated to Different Light Environments
Author: ZHANG Shou Ren*, MA Ke Ping, CHEN Ling Zhi
Journal of Integrative Plant Biology 2002 44(10): 1225-1232
      
    

There was heterogeneous distribution in stomatal conductance(gs)and stomatal aperture for both high and low light leaves of Ligustrum sinense Lour. in four designated positions within a leaf. Linear or exponential or polynomial relationships between gs and stomatal aperture were found when regression of gs and stomatal aperture was established. Statistical analysis revealed that the relationship between gs and stomatal aperture for high light leaves was more significant than that of low light leaves. A linear relationship between gs and stomatal aperture existed in both positions 1 and 3 for both high and low light leaves. The stomatal density of the low light leaves was much lower than that of the high light leaves. The sensitivity of stomata to changing environment for high light leaves was higher than that for low light leaves, which may also relate to the higher stomatal density for the high light leaves.

适应不同光环境小蜡叶片气孔导度、气孔开度和气孔密度的时空变化
张守仁* 马克平 陈灵芝

(中国科学院植物研究所植被数量生态学开放研究实验室,北京100093)


摘要: 对生长在强光环境和弱光环境小蜡叶片的气孔参数测定发现:气孔导度和气孔开度在4个取样部位存在异质性分布。气孔导度和气孔开度经回归分析呈线性、指数或多项式分布。统计分析表明:强光下的叶片气孔导度和气孔开度的相关性明显高于弱光环境叶片的数值。无论强光环境还是弱光环境下的叶片,在取样部位编号为1和3,其气孔导度和气孔开度均存在显著的线性关系。弱光环境下叶片的气孔密度要远低于强光环境下的叶片。强光环境下叶片对变化环境的敏感性要大于弱光环境下的叶片,这可能与强光环境叶片具有较高的气孔密度有关。
关键词: 适应;气体交换;光环境;显微镜技术;气孔异质性

通讯作者。Tel:+86-82599519;E-mail:<zsr @ ns.ibcasac.cn>;<zhangsrcn @ hotmail.com>.

Abstract (Browse 2626)  |  Full Text PDF       
Genetic Relationships Among Soluble Carbohydrates, Anthocyanins and Growth Characteristics in Leymus (Gramineae) Detected with Molecular Markers 
Author: HU Zan Min*, Steven R. LARSON, Thomas A. JONES, Richard R
Journal of Integrative Plant Biology 2002 44(10): 1173-1181
      
    

Low temperature soluble carbohydrate accumulations are commonly associated with anthocyanin coloration, attenuated growth and cold adaptation of cool season grasses. The vrn-1 gene has potent effects on vernalization requirement, growth, and soluble carbohydrate accumulations of the winter annual Triticeae species. Two hundred and four unmapped AFLP markers and genome specific DNA markers genetically linked to the vrn-1 gene were used to detect QTL controlling soluble carbohydrate accumulations, anthocyanin coloration and growth characteristics in a segregating population derived from open pollinated Leymus cinereus×L. triticoides hybrids. These perennial Triticeae grasses are distinguished by adaptation and growth habit. As expected, positive trait correlations and pleiotropic gene effects were detected for soluble carbohydrate accumulations and anthocyanin coloration. Likewise, positive trait correlations and pleiotropic gene effects were detected for tillering, leaf development, leaf growth, regrowth and rhizome spread. However, soluble carbohydrate accumulations were not associated with attenuated growth. In fact, several DNA marker alleles, including one near vrn-Xm1, had positive effects on soluble leaf carbohydrate concentrations and low temperature growth. The corresponding DNA marker near vrn-Ns1 had more specific effects on tillering. We speculate that vrn-1 exerts quantitative effects on low temperature soluble leaf carbohydrate accumulations and growth habit of the perennial Leymus. However, a number of other DNA markers displayed highly significant effects on soluble carbohydrate accumulations and various growth characteristics. Findings indicate that anthocyanin coloration may be a useful phenotypic marker for soluble carbohydrate accumulation. Although variation for soluble carbohydrates was not associated with attenuated growth in this population, this trait was under genetic control.

用分子标记研究禾本科赖草属植物可溶性碳水化合物、色素积累和生长特性之间的遗传相关性
胡赞民1,2* Steven R.LARSON 2* Thomas A.JONRS2 Richard R-C.WANC 2

(1.中国科学院遗传与发育生物学研究所,北京!100101;2. U.S.Department of Agriculture, Agriculture Research Service,Research Laboratory Utah State Unixersity,Logan,UT 84322-6300,USA)

摘要: 在低温条件下,冷季草的可溶性碳水化合物通常与紫色素的积累、生长的缓慢性及对寒冷的适应性具有相关性. Vrn-1基因对一年生冬性禾本科物种的春化、生长和可溶性碳水化合物的积累有重要作用.本研究以Leymus cinereus×L. triticoides 的杂种F 1开放授粉获得的F 2群体为材料,用204个未定位的AFLP分子标记和几个基因组特定的与 vrn-1相连锁的DNA标记检测了控制可溶性碳水化合物的积累、紫色素的积累和生长特性等几个数量性状的QTL.根据生长特性和适应性可将 Leymus cinereusL. triticoides区分开来.研究表明可溶性碳水化合物与紫色素的积累呈正相关,而且有关基因对这两种性状具有多效性.与之相类似,分蘖、叶发育、叶生长、草被剪后的再生长和地下茎的蔓延性这些性状之间也呈正相关,控制这些性状的基因具有多效性.但是可溶性碳水化合物的积累与生长的缓慢性无相关性.有几个分子标记包括与vrn-Xm1 邻近的一个分子标记对叶可溶性碳水化合物的浓度和低温生长具有正效应.而与vrn-Ns1邻近的一个DNA标记对分蘖具有更加特别的效应.我们推测vrn-1对多年生赖草低温下叶可溶性碳水化合物的积累及生长习性具有数量效应.发现几个DNA标记对可溶性碳水化合物的积累及多个生长特性有较强的作用.研究结果暗示在赖草中紫色素的积累可作为可溶性碳水化合物积累的形态标记.

关键词: 赖草属;分子标记;遗传相关性

通讯作者。E-mail: <zmhu @ genetics.ac.cn>.

Abstract (Browse 2001)  |  Full Text PDF       
Signal Transduction from Water Stress Perception to ABA Accumulation
Author: JIA Wen Suo, XING Yu, LU Cong Ming, ZHANG Jian Hua*
Journal of Integrative Plant Biology 2002 44(10): 1135-1141
      
    

To cope with unpredictably environmental perturbations and sometimes stresses, plants have evolved with some mechanisms so that these developing stresses can be sensitively perceived and the physiology can be rapidly regulated. Such perception and regulation can be a kind of feed forward mechanism and may involve many biochemical and physiological processes and/or the expression of many genes. Although many dehydration responsive genes have been identified, much fewer of their functions have been known. Such stress induced responses should include the initial perception of the dehydration signal, then the complicated signal transduction and cellular transmission until to the final gene activation or expression. As an important plant stress hormone abscisic acid (ABA) mediates many such responses. We believe that starting from the initial perception of dehydration to the gene expression leading to the stress induced ABA biosynthesis is the most important stress signal transduction pathway among all the plant responses to stresses. Identification of the genes involved and understanding their roles during stress perception and physiological regulation shall be the most important and interesting research field in the coming years.

从水分胁迫的识别到ABA积累的细胞信号转导
贾文锁1  邢 宇1 卢从明2  张建华3*

(1. 中国农业大学园艺系,北京100094;2. 中国科学院植物研究所光合作用研究中心,北京100093;
3.  香港浸会大学生物系,香港)

摘要: 由于植物在生长和发育过程中不可避免地要遭受各种环境胁迫的影响,植物只有通过对环境胁迫的快速感知和主动反应才得以生存和发展.植物这种对环境胁迫的快速感知和主动反应体现在环境胁迫下植物可以通过一系列基因的表达调控来实现各种抗逆的生理生化反应.虽然得以鉴定的水分胁迫应答基因越来越多,但其中只有极少的基因在抗逆中的基本功能已得到初步认识.从细胞对水分胁迫原初信号的感知到基因表达调控包括了一系列复杂的细胞逆境信息传递过程.脱落酸(abscisic acid, ABA)作为重要的细胞逆境信号物质介导了一系列基因表达,因此从细胞对水分胁迫原初信号的感知到编码ABA生物合成关键酶基因的表达是一条最为关键的细胞逆境信息传递途径.逆境应答基因功能的鉴定以及对整个细胞信号传递过程中详尽的分子机制的了解无疑是今后最有趣的也是最为重要的研究课题.

关键词: 水分胁迫;识别;转导;脱落酸

通讯作者。E-mail: <jzhang @ hkbu.edu.hk>.

Abstract (Browse 2366)  |  Full Text PDF       
ubtree, TASS and an Analysis of the Genus Caragana
Author: ZHANG Ming Li*, Pauline Y. LADIGES, Gareth NELSON
Journal of Integrative Plant Biology 2002 44(10): 1213-1218
      
    

Subtree analysis and three area satements (TASS) procedure are used to deal with the area relationship in historical biogeography. On the basis of the taxon cladogram, the procedure could identify and eliminate the paralogy node and determine the informative subtree. The area relationships are generally illustrated in several subtrees and the consensus tree. The distribution pattern of the genus Caragana comprising about 72 species and occurring in 13 areas, was analyzed by using subtree method and TASS procedure in this study. The results showed seven subtrees representing the area relationships of section and series of the genus, and the consensus tree provided the 13 area relationships. These results are congruent with our former result using component analysis for Caragana.

子树分析和TASS程序及其在锦鸡儿属植物中的应用
张明理1* Pauline Y.LADICES 2,Gareth NELSON 2(1.  中国科学院植物研究所,北京100093; 墨尔本大学植物学院,维多利亚3010,澳大利亚)

摘要: 子树分析和三分法(TASS)程序是历史生物地理学中分布区关系的一种分析途径.它以分类群分支图为基础,以剔除其中分布区关系相悖理的结点并确定具信息的子树为目标,以便更有效地利用分布区信息.对分布区关系可以用若干子树和一个分布区分支图来表示.对锦鸡儿属(Caragana)植物72种和13个分布区的子树分析和TASS程序运算后,得到7个具信息的子树,它们分别表达了锦鸡儿属属内组、系所具有的分布区关系.属的分布区分支图也表达了13个分布区的关系.与以前我们对本属成分分析的结果相吻合.

关键词: 子树;TASS 程序;锦鸡儿属;地理分布式样

通讯作者。E-mail: <zhangml @ ns.ibcas.ac.cn>.

Abstract (Browse 2121)  |  Full Text PDF       
Enzymatic Degradation of Parvifloside
Author: JIN Jian Ming, LIU Xi Kui, TENG Rong Wei, YANG Chong Ren*
Journal of Integrative Plant Biology 2002 44(10): 1243-1249
      
    

Parvifloside (1), a new furostanol pentaglycoside, was isolated from the fresh rhizomes of Dioscorea parviflora C. T. Ting. On the basis of spectroscopic and chemical methods, its structure was elucidated as (25R) 26 O β glucopyranosyl furost 5 en 3β,22ξ,26 triol 3 O βD glucopyranosyl (1→3) βD glucopyranosyl (1→4) [αL rhamnopyranosyl (1→2)] βD glucopyranoside. Six prosapogenins (2-7) were obtained from the enzymatic degradation of 1 by cellulase, but only 3 and 4 were obtained by β glucosidase. The structures of all compounds were determined by spectroscopic data. The activity of the isolated compounds on deformation of mycelia germinated from Pyricularia oaryzae P 2b conidia was evaluated.

小花盾叶薯蓣甙的酶降解
金建明 刘锡葵 滕荣伟 杨崇仁*

(中国科学院昆明植物研究所植物化学与西部植物资源持续利用国家重点实验室,昆明650204)

摘要: 从小花盾叶薯蓣 (Dioscoreaparviflora C.T .Ting)的新鲜根状茎中分离到一个新的呋甾烷型配糖体 ,命名为小花盾叶薯蓣甙 (parvifloside) (1) ,其结构通过波谱和化学方法鉴定为 :(2 5R)-2 6-O-β-glucopyranosyl-furost-5-en-3β ,2 2 ξ ,2 6-triol 3-O-β-D-glucopyranosyl (1→ 3)-β-D-glucopyranosyl (1→ 4 )-[α-L-rhamnopyranosyl (1→ 2 ) ]-β-D-glucopyra noside。化合物 1在纤维素酶粗酶和 β_葡萄糖苷酶中进行水解 ,得到降解产物 2 - 7。对 1的酶解现象进行了讨论。同时 ,对所分离的甾体皂甙的抗稻瘟霉菌活性进行了初步筛选。

关键词: 小花盾叶薯蓣;小花盾叶薯蓣甙;甾体皂甙;酶降解

通讯作者。Tel.:+86-871-5223424;Fax: +86-871-5150124;E -mail: <cryang @ public.km.yn. cn>;<glycoside @ maul.kib.ac.cn>.

Abstract (Browse 2200)  |  Full Text PDF       
Cloning and Differential Expression of a 1-Aminocyclopropane-1-Carboxylate Synthase cDNA from Peach
Author: JIN Yong-Feng, ZHU Li-Cheng, ZHANG Yao-Zhou and ZHANG Shang-Long
Journal of Integrative Plant Biology 2002 44(10): 1182-1187
      
    

The ACC synthase is the key enzyme in ethylene biosynthesis and fruit ripening. To study the mechanism of ACC synthase in peach ( Prunus persica (L.) Batsch) fruit ripening, we cloned a full length cDNA of ACC synthase pacs from peach using 5′/3′ RACE PCR. The nucleic acid sequence of pacs was 1848 bp, containing 177bp of 5′untranslated sequence, 1449 bp of an open reading frame, and 219 bp of 3′untranslated sequence (excluding the stop codon TAA). The pacs open reading frame encoded a 483 amino acid polypeptide with a predicted size of 54 kD and a calculated PI of 6.43. The deduced protein from ACC synthase cDNA pacs had 65%, 70%, 75%, and 90% homology with the other deduced proteins from tomato (S19677), plum (AB031026), papaya (U68216) and apple (AB034993), which contained the active site of ACC synthase SLSKDMGFPGFR conserved among these plant ACC synthases. RNA based PCR amplification combined with hybridization analysis with pacs and another ACC synthase cDNA pacs12 (AF467782) cloned by us before as probes, indicated that expression patterns of both clones were very similar. mRNAs of both clones expressed in the alabastrum and petal, and were induced after ethylene treatment. Wounding and IAA treatments could induce ACC synthase expression of both clones in the leaves. However, the wounding treatment of leaves has induced more abundant pacs ACC synthase expression than that of pacs12 . Pacs mRNA expressed in both green mature and ripening fruit, while pacs12 mRNA was little or undetectable in green mature fruit, but apparent in ripening fruit. Both clone mRNAs accumulated more in leaves (following wounding and IAA treatments) and flowers than in fruits.

桃ACC合酶基因的分离及其差异表达
金勇丰1 朱立成1  张耀洲1  张上隆2

(1. 浙江大学生物化学研究所;2. 浙江大学园艺系,杭州310029)

摘要: 利用 5′/ 3′RACEPCR技术 ,从桃 (Prunuspersica (L .)Batsch)果实中克隆了植物乙烯生物合成的关键酶———ACC合酶的全长cDNA pacs,对pacs基因进行全序列测定表明 ,该基因全长 184 8个碱基 ,编码区为 14 4 9个碱基 ,5′端有 177个碱基的非编码区序列 ,3′端有 2 19个碱基的非编码区序列 (不包括终止密码子TAA)。pacs基因编码区共编码 4 83个氨基酸 ,蛋白质大小为 5 4kD ,等电点为 6 .4 3。pacs与番茄 (S196 77)、梅 (AB0 310 2 6 )、番木瓜 (U6 82 16 )、苹果(AB0 34993)等其他植物ACC合酶cDNA氨基酸序列同源性分别为 6 5 %、70 %、75 %、90 % ,并存在与这些ACC合酶氨基酸的活性位点保守序列SLSKDMGFPGFR。RT-PCR结合杂交分析表明 ,pacs和我们以前克隆的桃ACC合酶cDNApacs12 (AF4 6 7782 )在叶片和花中基因表达模式基本一致 ,伤处理和IAA均能诱导叶片pacspacs12基因的表达 ,但pacs在伤处理叶片的表达水平比pacs12高 ;pacs和pacs12基因在果实表达有所不同 ,pacs在绿熟和成熟果实中均有表达 ,而pacs12在绿熟果实中基本检测不到 ,在成熟果实中才有表达 ,两者在果实中的表达水平比伤处理和IAA处理叶片和花中要低。

关键词: 桃ACC合酶;基因分离;差异表达

Abstract (Browse 2135)  |  Full Text PDF       
Discovery and Application of the Companion Fungus Related to Sclerotial Formation from Hyphae of Grifola umbellata
Author: GUO Shun Xing*, WANG Qiu Ying, ZHUANG Wen Ying, ZHANG Ji Hui, XING Xiao Ke
Journal of Integrative Plant Biology 2002 44(10): 1239-1242
      
    

The companion fungus (Grifola sp.) related to sclerotial formation from hyphae of Grifola umbellata (Pers.) Pilát was isolated from the cavity associated with sclerotia of G. umbellata in natural condition. Experimental results showed that the pure culture of G. umbellata was unable to form sclerotia, whereas sclerotia produced easily in flasks or on trunks when the companion fungus was inoculated together with strain of G. umbellata. The companion fungus is critical for sclerotium formation from hyphae of G. umbellata. Morphological differences were found in cultures of the two fungi. The companion fungus possesses thin walled narrow hyphae, while G. umbellata is of wider hyphae which are either thick or thin walled.

红豆杉一内生真菌化学成分的研究
张集慧 郭顺星* 杨峻山 肖培根
(中国医学科学院中国协和医科大学药用植物研究所,北京100094)

 摘要: 从药用植物红豆杉 (Taxuschinensis (Pilg.)Rehd .)的内生真菌———粘帚霉属真菌 (Gliocladium sp .,简称F菌 )菌丝体中分离到 3个化合物 ,根据光谱方法确定了它们的结构。其中 ,(2 0S ,2 2S)-4a-同-2 2-羟基-4-氧杂麦角甾-7,2 4 (2 8)-二烯-3-酮为新化合物 ,化合物 4 ,8,12 ,16_四甲基_1,5 ,9,13_四氧杂环十六烷-2 ,6 ,10 ,14-四酮为首次从该属真菌中分离到 ,6 ,9-环氧麦角甾-7,2 2-二烯-3-羟基为首次从F菌中分离到。

关键词: 红豆杉;(20S,22S&)-4a-同-22-羟基-4-氧杂麦角甾-7,24(28)-二烯-3-1酮;化学成分;粘帚霉属

通讯作者。Tel.:010-62899729.E-mail: <sxguo @ hotmail.com>

Abstract (Browse 2754)  |  Full Text PDF       
The Arabidopsis AS2 Gene Encoding a Predicted Leucin zipper Protein Is Required for the Leaf Polarity Formation
Author: XU Yi, SUN Yue, LIANG Wan Qi, HUANG Hai
Journal of Integrative Plant Biology 2002 44(10): 1194-1202
      
    

The asymmetric leaves2 ( as2 ) is a classical Arabidopsis thaliana(L.) Heynh. mutant that shows leaf lobes and leaflet like structures from the petioles of leaves. Genetic and molecular analyses have demonstrated that the AS2 function is required for repression of meristem related homeobox genes in leaves. In this study, we describe phenotypic characterizations of new as2 alleles that are in the Landsberg erecta (Ler) genetic background. In addition to the as2 phenotypes reported previously, the new as2 mutants have some leaves with petiole growth underneath the leaf blade, showing a lotus leaf structure. More severe rosettes leaves of the as2 mutants form a filament like structure, reflecting a loss of the adaxial abaxial polarity in leaves. Among as2 mutants analyzed in different genetic backgrounds, only those that are in the Ler genetic background resulted in a high frequency of the lotus leaf structure. We have isolated the AS2 gene by map based gene cloning. The predicted AS2 protein contains a leucine zipper motif, and its N terminus shares high levels of sequence similarity to those of a group of predicted proteins with no known biological functions.AS2 transcripts were detected in leaves, flowers and fruits, but absent in stems, consistent with the mutant phenotypes.

拟南芥编码亮氨酸拉链蛋白的AS2基因在叶发育中具有控制极性建立的作用
徐 轶* 孙 越* 梁婉琪 黄 海**

(中国科学院上海生命科学研究院上海植物生理生态研究所植物分子遗传国家重点实验室,上海200032)

摘要: 在拟南芥 (Arabidopsisthaliana (L .)Heynh .)叶发育研究中 ,as2是一个经典突变体。as2典型的表型是叶片开裂或形成一种小叶状结构。遗传学和分子生物学实验证明 ,AS2基因具有抑制KNOX基因在叶中表达的功能。在本文中 ,我们着重研究了新得到的在Landsbergerecta (Ler)遗传背景下的as2突变体。除了前人报道过的as2表型外 ,新as2突变体的部分叶柄长在叶片的下方 ,形成一种荷叶状结构 ,更严重的甚至长成花丝状叶结构。这两种结构都反映了不正常的叶腹背轴极性分化。在我们所收集到的as2等位突变体中 ,只有在Ler背景下这两种结构才以高频率出现。我们通过图位克隆方法分离了AS2基因。该基因编码一个含有亮氨酸拉链结构的蛋白。在拟南芥中 ,AS2同源基因共 4 3个 ,除AS2外 ,其他基因的功能都不清楚。AS2在叶和花中表达 ,在茎中无表达 ,这种表达模式和as2突变体的表型是吻合的。

关键词: 拟南芥;ASYMMETRIC LEAVESI;ASYMMETRIC LEAVES2; ERECTA;亮氨酸拉链结构;极性建立

通讯作者。Tel.:+86-21-64042090;Fax:+86-21-64042385;E-mail:<hhuang @ iris.sipp.ac.cn>.

Abstract (Browse 2429)  |  Full Text PDF       
Modeling the Cysteine Rich Domain of Plant Metallothionein-like Protein
Author: HE Hong-Zhen, ZHU Chun-Ming, L|B Tun, ZHANG Ri-Qing, ZHAO Nan-Ming and LIU Jin-Yuan
Journal of Integrative Plant Biology 2002 44(10): 1155-1159
      
    

With the progress of plant genome research, more than 50 plant metallothionein like (MT-L) genes have been found, but only several MT-L proteins have been detected and no experimental structural information for MT-L proteins has been reported so far. Since detailed knowledge of the protein tertiary structure is required to understand its biological function, a method is needed to determine the structure of these proteins. In this study, the structural data of known mammal MT was used to determine the interatomic distance constraints of the CXC and CXXC motifs and the metal sulfur chelating cluster. Then several possible MT conformations were predicted using a distance geometry algorithm. The statistical analysis was used to select those with much lower target function values and lower conformation energies as the predicted tertiary structural models of the cysteine rich (CR) domains of these proteins. A suitable prediction method for modeling the CR domain of the plant MT-L protein was constructed. The accurately predicted result for the known structure of an MT protein from blue crab suggests that this method is practicable. The tertiary structures of CR domains of rape MT-L protein LSC54 was then modeled with this method.

植物类金属硫蛋白半胱氨酸富含区结构的建模
何红珍 朱春明 吕 暾 张日清 赵南明 刘进元*

(清华大学生物科学与技术系,生物膜与膜生物工程国家重点实验室,北京100084)

摘要: 详细了解蛋白质的三级结构信息有助于理解其生物学功能。随着植物基因组研究的进展 ,已发现了 50多个植物类金属硫蛋白 (Metallothionein-Like ,MT-L)基因。但至今只有少数几个MT-L蛋白得到了纯化 ,而其结构尚无报道 ,因此有必要建立分析这类蛋白结构特征的方法。本研究根据已知的哺乳动物MT的结构数据 ,分析得出了CXC、CXXC模式和金属 硫络合簇结构原子间的距离限制条件 ,并用距离几何算法计算得出预测蛋白可能的构象 ;然后通过统计分析筛选出目标函数值显著较小、构象能低的结构作为这些蛋白半胱氨酸富含区的预测结构 ,由此建成了适合于植物类金属硫蛋白半胱氨酸富含区的结构预测方法。从应用该方法正确地预测出了已知结构的蓝蟹MT的结构来看 ,该方法是可行的。并用该方法预测了油菜MT_L蛋白的半胱氨酸富含区的结构。

关键词: 植物类金属硫蛋白;半胱氨酸富含区;三级结构预测;距离几何算法

通讯作者. E-mail:<liujy @ mail.tsinghua.edu.cn>.

Abstract (Browse 2698)  |  Full Text PDF       
Mechanism of the Effect of Polyamines on the Activity of Tonoplasts of Barley Roots Under Salt Stress
Author: SUN Cheng, LIU You-Liang and ZHANG Wen-Hua
Journal of Integrative Plant Biology 2002 44(10): 1167-1172
      
    

The seeds of barley ( Hordeum vulgare L. cv. Jian 4) were soaked with 0.1 mmol/L putrescine (Put) and 0.5 mmol/L spermidine (Spd), and then the seedlings were treated with 200 mmol/L NaCl. The growth rate (GR), dry matter accumulation, distribution of ions, the amount of polyamines (PAs) bound to tonoplast proteins as well as lipid composition and the activity of tonoplast vesicles isolated from roots were investigated. The results showed that soaking with Put or Spd could retard salt injury, promote GR and dry matter accumulation, and increase K+/Na+ in the roots. Compared with NaCl treated plants, phospholipid content in root tonoplast rose by soaking with Put and Spd, while the level of galactose in lipids was decreased. Moreover, the ratio in noncovalently conjugated PA contents of (Spd+PAx (an unknown PA)) to (Put+Dap (diaminopropane)), and the total contents of covalently and noncovalently conjugated PAs were all increased. Statistical analysis indicated that the ratio of (Spd+PAx)to (Put+Dap) was significantly and positively correlated with the activities of membrane associated enzymes H+ ATPase and H+ PPase.

多胺浸种改善盐胁迫大麦根系液泡膜功能的机理
孙 诚 刘友良* 章文华
(南京农业大学,农业部作物生长调控重点实验室,南京210095)

摘要: 研究了0.1 mmol/L 腐胺 (Put) 和0.5 mmol/L 亚精胺 (Spd) 浸种对200 mmol/L NaCl胁迫下大麦(Hordeum vulgare L.)幼苗生长速率、干物质积累、离子分布、液泡膜蛋白结合多胺含量以及液泡膜膜脂组分与功能的影响.结果表明,Put和Spd浸种均可缓解盐胁迫对大麦幼苗的盐害,促进生长和干物质积累,降低大麦幼苗体内[Na+]/[K+].与盐处理的对照植株相比,Put和Spd浸种均可提高大麦幼苗根系液泡膜磷脂含量,降低糖脂结合半乳糖含量,而膜上非共价结合多胺含量Spd+PAx (一种未知多胺) 与 Put+Dap (二氨基丙烷)之比((Spd+PAx)/(Put+Dap))、共价和非共价结合多胺总量均上升.统计分析结果表明,液泡膜非共价结合多胺(Spd+PAx)/(Put+Dap)与H+-ATPase和H+-PPase活性呈显著正相关关系.

关键词: 大麦;盐胁迫;多胺;膜脂;膜结合酶

通讯作者。E-mail: <zwslsh @ public l.ptt.js.cn>

Abstract (Browse 2183)  |  Full Text PDF       
Mechanism of Oxidative Burst in Tobacco Leaves and Cells Induced by Palmin from Phytophthora palmi
Author: WANG Yi Hua, ZHANG Hong Ming, YU Zhong Lian, CHEN Jia*, WANG Xue Chen
Journal of Integrative Plant Biology 2002 44(10)
      
    In order to reveal the signaling pathways triggered by elicitor in plant microbe interactions, the mechanisms of hypersensitive necrosis responses in Nicotiana tabacum L. cv. Gexin Ⅲ induced by palmin were studied at molecular and cellular level. The burst of superoxide, intercellular diffusion of hydrogen peroxide and process of cell death induced by palmin were investigated in tobacco plants by biochemical methods and Confocal microscopy. The results showed that a large amount of O·[KG-*5]-2 was rapidly generated in tobacco cell elicited by palmin as a result of activation of NADPH oxidase, and the O·[KG-*5]-2 was dismutated into H2O2 immediately by superoxide dismutase (SOD). Accumulation and intercellular diffusion of H2O2 were shown to be a trigger for hypersensitive cell death; and Ca2+and some specific protein kinase were also shown to be involved in the activation of oxidative burst in tobacco cell induced by palmin.
Abstract (Browse 2000)  |  Full Text PDF       
 

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