March 2006, Volume 48 Issue 3, Pages 249-369.

Cover Caption:
Anatomical observation of a mutant floret and fluorescence microscopic observation of pollen tubes’ entering the stigmas reveals that some mutant florets have one more stigma compared with the wild type, and that the pollen tubes normally enter the stigmas in fs-202R just as seen in the wide type. See pages 307-314 for more details.


          Research Articles
Carbon Monoxide Alleviates Salt-Induced Oxidative Damage in Wheat Seedling Leaves
Author: Ben-Kai Huang, Sheng Xu, Wei Xuan, Ming Li, Ze-Yu Cao, Kai-Li Liu, Teng-Fang Ling and Wen-Biao Shen
Journal of Integrative Plant Biology 2006 48(3)
DOI: 10.1111/j.1744-7909.2006.00220.x
    Carbon monoxide (CO), a by-product released during the degradation of heme by heme oxygenases (EC in animals, is regarded as an important physiological messenger or bioactive molecule involved in many biological events that has been recently reported as playing a major role in mediating the cytoprotection against oxidant-induced lung injury. In the present study, we first determined the protective effect of exogenous CO against salt-induced oxidative damage in wheat seedling leaves. Wheat seedlings treated with 0.01 mol/L hematin as the CO donor demonstrated significant reversal of chlorophyll decay, dry weight, and water loss induced by 300 mmol/L NaCl stress. Interestingly, the increase in lipid peroxidation observed in salt-treated leaves was reversed by 0.01μmol/L hematin treatment. Time-course analyses showed that application of 0.01μmol/L hematin enhanced guaiacol peroxidase, superoxide dismutase, ascorbate peroxidase and catalase activities in wheat seedling leaves subjected to salt stress. These effects are specific for CO because the CO scavenger hemoglobin (1.2 mg/L) blocked the actions of the CO donor hematin. However, higher concentration of the CO donor (1.0μmol/L) did not alleviate dry weight and water loss of salt-stressed wheat seedlings. These results suggest that exogenous application of low levels of a CO donor may be advantageous against salinity toxicity.(Author for correspondence.Tel (Fax): 025 8439 6673;E-mail:
Abstract (Browse 3773)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
Global Warming: Can Existing Reserves Really Preserve Current Levels of Biological Diversity?
Author: Mai-He Li, Norbert Kräuchi and Su-Ping Gao
Journal of Integrative Plant Biology 2006 48(3)
DOI: 10.1111/j.1744-7909.2006.00232.x
    Paleoecological evidence and paleoclimatic records indicate that there was a plant poleward migration in latitude and an upward shift in elevation with increased temperatures after the last glaciation. Recent studies have shown that global warming over the past 100 years has been having a noticeable effect on living systems. Current global warming is causing a poleward and upward shift in the range of many plants and animals. Climate change, in connection with other global changes, is threatening the survival of a wide range of plant and animal species. This raises the question: can existing reserves really preserve current levels of biological diversity in the long term given the present rapid pace of climate change? The present paper deals with this question in the context of the responses of plants and animals to global climate change, based on a literature review. Consequently, we recommend expanding reserves towards the poles and/or towards higher altitudes, to permit species to shift their ranges to keep pace with global warming.(Author for correspondence.E-mail:
Abstract (Browse 4029)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
Differentiation of a Miniature Inverted Transposable Element (MITE) System in Asian Rice Cultivars and Its Inference for a Diphyletic Origin of Two Subspecies of Asian Cultivated Rice  
Author: Hao Hu, Jie Mu, Hong-Ji Zhang, Yue-Zhi Tao and Bin Han
Journal of Integrative Plant Biology 2006 48(3): 260-267
DOI: 10.1111/j.1744-7909.2006.00221.x
    In the present study, we report a survey on a Miniature Inverted Transposable Element (MITE) system known as mPing in 102 varieties of Asian cultivated rice (Oryza sativa L.). We found that mPing populations could be generalized into two families, mPing-1 and mPing-2, according to their sequence structures. Further analysis showed that these two families of mPing had significant bias in their distribution pattern in two subspecies of rice, namely O. sativa ssp. japonica and indica. O. sativa japonica has a higher proportion of mPing-1 as a general trait, whereas O. sativa indica has a higher proportion of mPing-2. We also examined the mPing system in a doubled haploid (DH) cross-breeding population of jingxi 17 (japonica) and zhaiyeqing 8 (indica) varieties and observed that the mPing system was not tightly linked to major subspecies-determining genes. Furthermore, we checked the mPing system in 28 accessions of Asian common wild rice O. rufipogon and found the mPing system in O. rufipogon. The distribution pattern of the mPing system in O. rufipogon indicated a diphyletic origin of the Asian cultivated rice O. sativa species. We did not find the mPing system in another 20 Oryza species. These results substantiated a previous hypothesis that O. rufipogon and O. nivara species were the closest relatives of O. sativa and that the two extant subspecies of O. sativa were evolved independently from corresponding ecotypes of O. rufipogon.(Author for correspondence.Tel: 021 6484 5260; Fax: 021 6482 5775; E-mail:
Abstract (Browse 3227)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
Variant Scaling Relationship for Mass-Density Across Tree-Dominated Communities
Author: Hai-Tao Li, Xing-Guo Han and Jian-Guo Wu
Journal of Integrative Plant Biology 2006 48(3)
DOI: 10.1111/j.1744-7909.2006.00222.x
    The past few decades have seen a resurgence of interest in biological allometry. Specifically, a number of recent studies has suggested a –4/3 invariant scaling relationship between mass and density that is universally valid for tree-dominated communities, regardless of their phyletic affiliation or habitat. In the present study, we test this scaling relationship using a comprehensive forest biomass database, including 1 266 plots of six biomes and 17 forest types across China. The present study shows that the scaling exponent of the mass-density relationship varies across different tree-dominated communities and habitats. This great variability in the scaling exponent makes any generalization unwarranted. Although inappropriate regression methods can lead to flawed estimation of the scaling exponent, inconsistency of theoretical framework and empirical patterns may have undermined the validity of previous work.(Author for correspondence.Tel: +86 10 6488 8996; Fax: +86 10 6485 9781; E-mail:
Abstract (Browse 3024)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
Climate Signals from Tree Ring Chronologies of the Upper and Lower Treelines in the Dulan Region of the Northeastern Qinghai-Tibetan Plateau
Author: Lu-San Liu, Xue-Mei Shao and Er-Yuan Liang
Journal of Integrative Plant Biology 2006 48(3)
DOI: 10.1111/j.1744-7909.2006.00158.x
    The radial growth of trees in mountainous areas is subject to environmental conditions associated with changes in elevation. To assess the sensitivity of tree-ring growth to climate variation over a wide range of elevations, we compared the chronological characteristics of Sabina przewalskii Kom. and their relationships with climatic variables at the upper and lower treelines in the Dulan region of the northeastern Qinghai-Tibetan Plateau. It was found that the radial growth in this region was controlled primarily by precipitation in late spring and early summer (from May to June). In addition, a higher temperature from April to June could intensify drought stress and lead to narrow tree rings. The significant similarity in climate-tree growth relationships at both the upper and lower treelines indicated that tree rings of S. przewalskii in this region are able to provide common regional climate information. However, the chronologies at the lower forest limits showed a higher standard deviation and more significant correlations with climatic factors, suggesting that the radial growth there was more significantly influenced by climate variation. The first principal component of the four chronologies showed a common growth response to local climate. The second principal component showed a contrasting growth response between different sampling sites. The third principal component revealed different growth patterns in response to altitudinal variation. Further analysis indicated that the precipitation in late spring and early summer controlled the growth of S. przewalskii on a regional scale and that other factors, such as microenvironment at the sampling sites, also affected the strength of the climatic response of tree growth.(Author for correspondence.Tel: 010 6485 6495; Fax: 010 6485 1844; E-mail:
Abstract (Browse 3330)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
Growth, Gas Exchange, Abscisic Acid, and Calmodulin Response to Salt Stress in Three Poplars
Author: Yu Chang, Shao-Liang Chen, Wei-Lun Yin, Rui-Gang Wang, Yan-Feng Liu, Yong Shi, Yuan-Yuan Shen, Yue Li, Jie Jiang and Yue Liu
Journal of Integrative Plant Biology 2006 48(3)
DOI: 10.1111/j.1744-7909.2006.00194.x
    In the present study, we investigated the effects of increasing salinity on growth, gas exchange, abscisic acid (ABA), calmodulin (CaM), and the relevance to salt tolerance in seedlings of Populus euphratica Oliv. and cuttings of P. “pupularis 35-44” (P. popularis) and P. × euramericana cv. I-214 (P. cv. Italica). The relative growth rates of shoot height (RGRH) for P. cv. Italica and P. popularis were severely reduced by increasing salt stress, whereas the growth reduction was relatively less in P. euphratica. Similarly, P. euphratica maintained higher net photosynthetic rates (Pn) and unit transpiration rates (TRN) than P. cv. Italica and P. popularis under conditions of higher salinity. Salinity caused a significant increase in leaf ABA and CaM in the three genotypes after the onset of stress, but NaCl-induced ABA and CaM accumulation was more pronounced in P. euphratica, suggesting that P. euphratica plants are more sensitive in sensing soil salinity than the other two poplars. Furthermore, P. euphratica maintained relatively higher ABA and CaM concentrations under conditions of high salinity. The higher capacity to synthesize stress signals, namely ABA and CaM, in P. euphratica and the contribution of this to the salt resistance of P. euphratica are discussed.(Author for correspondence.Tel: 010 6233 8129; Fax: 010 6233 7855; E-mail: )
Abstract (Browse 3151)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
Biochemical Properties and Inhibition Kinetics of Phosphatase from Wheat Thylakoid Membranes
Author: Mei-Juan Fei, Jian-Sheng Chen and Xiao-Yun Wang
Journal of Integrative Plant Biology 2006 48(3)
DOI: 10.1111/j.1744-7909.2006.00195.x
    A phosphatase that hydrolyses phosphate monoesters has been isolated from wheat thylakoid membranes. Biochemical properties and inhibition kinetics of the phosphatase were investigated using several ions, organic solvents, and inhibitors. Wheat (Triticum aestivum L. cv. PH82-2-2) thylakoid membrane phosphatase activity was activated by Mg2+, Ca2+, and Fe2+ and was inhibited by Mn2+ and Cu2+. For example, enzyme activity was activated 34.81% by 2 mmol/L Mg2+, but was inhibited 22.3% and 8.5% by 2 and 1 mmol/L Cu2+, respectively. Methanol, ethanol and glycol were all able to activate enzyme activity. Enzyme activity was activated 58.5%, 48.2%, and 8.7% by 40% ethanol, methanol and glycol, respectively. From these results, it can be seen that the degree of activation of the phosphatase was greatest for ethanol and the type of activation was uncompetitive. Moreover, the activity of the thylakoid membrane phosphatase was inhibited by molybdate, vanadate, phosphate, and fluoride and the type of inhibition produced by these elements was uncompetitive, non-competitive, competitive and mixed, respectively.(Author for correspondence.Tel: 0538 824 2656 8430; E-mail:
Abstract (Browse 2709)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
Evidence of Dichogamy in Santalum album L.
Author: Guo-Hua Ma, Eric Bunn, Jing-Feng Zhang and Guo-Jiang Wu
Journal of Integrative Plant Biology 2006 48(3)
DOI: 10.1111/j.1744-7909.2006.00201.x
    Flowering, fruit set, embryological development, and pollination trials were investigated in Santalum album L. Each ovary may have three to four ovules. Microsporogenesis and megasporogenesis in the same flower were synchronized at the earlier stages of flower development. However, at anthesis, when pollen was mature, the magaspore had developed only to the stage of a one- to two-nucleus embryo sac. As the eight-nucleus embryo sac developed, some mamelon cells began to undergo programmed cell death, forming holes into which the eight-nucleus embryo sacs extended, becoming “N” or “S” shaped. The development from a two-nucleus embryo sac to a matured eight-nucleus embryo sac lasted up to 10 d. Fruit-set from open pollination was less than 2%. The endosperm develops prior to division of the zygotic embryo and one to three embryos and endosperms were formed in the same fruit. A mature seed usually germinates to produce one seedling; however, two and three seedlings from one seed were also observed, albeit at a low frequency. Pollination trials showed that no seed sets when inflorescences were covered with a bag; however, artificial pollination could improve fruit set. Our pollination trials and embryological studies proved that the flower of S. album is dichogamous and fruit set has high heterozygosity.(Author for correspondence.Tel: 020 3725 2993; Fax: 020 3725 2703;
Abstract (Browse 3055)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
Phenotypic Characterization of a Female Sterile Mutant in Rice
Author: Shuang-Cheng Li, Li Yang, Qi-Ming Deng, Shi-Quan Wang, Fa-Qiang Wu and Ping Li
Journal of Integrative Plant Biology 2006 48(3)
DOI: 10.1111/j.1744-7909.2006.00228.x
    A female sterile mutant, derived from a spontaneous mutation, was first discovered in rice (Oryza sativa L. ssp. indica) restorer line 202R. With normal flowering, the mutant exhibits an extremely low seed-setting rate. When the mutant is crossed as a pollen donor, the seeds set normally; whereas when it is used as a pollen receiver, no seeds are obtained even with mixed pollen grains of different varieties sprinkled over the stigmas. The floret of the mutant, consisting of six stamens and one pistil, looks the same as that of the wild type in the male-female organs, except that less than 10% of the mutant florets have three stigmas on the ovary. Although the mutant has a low seed-setting rate, its pollen fertility is approximately 87.1%, which is equal to that of the wild type. In addition, more than 90% of the mature embryo sacs of the mutant have complete inner structures. At every stage after pollination, the sperm, embryo, and endosperm are not found in the mutant embryo sac, whereas the disintegration of the egg cell that does not accomplish fertilization is visible. Through observations with a fluorescence microscope, we have found that the pollen grains germinate normally, whereas the pollen tube abnormally elongates in the style-transmitting tissue. The mutant pollen tubes display various defects in the style, such as slower elongation, conversed elongation, distorted elongation, swollen tips, or branched tips. As a result, the growth of the pollen tubes ceases in the style, and, therefore, the pollen tubes cannot reach the embryo sac and the process of double fertilization is blocked. Based on these observations, we conclude that this mutant, designated as fs-202R, is a novel type of female sterile mutation in rice, which causes the arrest of the elongation of the pollen tube.(Author for correspondence.Tel: 028 8272 2497; Fax: 028 8272 6875; E-mail:
Abstract (Browse 3208)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
Construction and Characterization of a cDNA Library from the Pulp of Cara Cara Navel Orange (Citrus sinensis Osbeck)
Author: Neng-Guo Tao, Juan Xu, Yun-Jiang Cheng and Xiu-Xin Deng
Journal of Integrative Plant Biology 2006 48(3)
DOI: 10.1111/j.1744-7909.2006.00225.x
    A cDNA library was constructed and characterized from the pulp of Cara Cara navel orange (Citrus sinensis Osbeck) at different stages of ripening. Tittering results revealed that approximately 5.086×105 independent clones were included in this library. Electrophoresis gel results of 15 randomly selected clones revealed that the size of the insertion fragments ranged from 400 bp to 2 kb, with an average size of 900 bp. Sequencing results of 150 randomly picked clones showed that the recombination rate was 94%. During subsequent sequence analysis, 41 of 139 clones failed to be identified and the amino sequence of 71 clones shared less than 30% identity with related plants in GenBank. Of 27 clones whose amino sequences shared more than 60% identity with other related plants in GenBank, 17 clones showed an 80% identity with the corresponding candidate genes of citrus. The clone recognized as the type III metallothionein-like (MT) gene was observed to occur 13 times, indicating that the protein may play an important role in fruit development and ripening.(Author for correspondence.Tel: 027 8728 1712; Fax: 027 8728 0016; E-mail:
Abstract (Browse 2941)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
Molecular Tagging and Mapping of Quantitative Trait Loci for Lint Percentage and Morphological Marker Genes in Upland Cotton
Author: Wang-Zhen Guo, Guo-Jia Ma, Yi-Chao Zhu, Chen-Xin Yi and Tian-Zhen Zhang
Journal of Integrative Plant Biology 2006 48(3)
DOI: 10.1111/j.1744-7909.2006.00174.x
    Using 219 F2 individuals developed by crossing the genetic standard line TM-1 and the multiple dominant marker line T586 in Gossypium hirsutum L., a genetic linkage map with 19 linkage groups was constructed based on simple sequence repeat (SSR) markers. Compared with our tetraploid backboned molecular genetic map from a (TM-1×Hai 7124)×TM-1 BC1 population, 17 of the 19 linkage groups were combined and anchored to 12 chromosomes (sub-genomes). Of these groups, four morphological marker genes in T586 had been mapped into the molecular linkage map. Meanwhile, three quantitative trait loci for lint percentage were tagged and mapped separately on the A03 linkage group and chromosome 6.(Author for correspondence.Tel (Fax): 025 8439 5307; E-mail:
Abstract (Browse 3066)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
The Putative Ser/Thr Protein Kinase Gene GmAAPK from Soybean is Regulated by Abiotic Stress
Author: Guang-Zuo Luo, Yong-Jun Wang, Zong-Ming Xie, Jun-Yi Gai, Jin-Song Zhang and Shou-Yi Chen
Journal of Integrative Plant Biology 2006 48(3)
DOI: 10.1111/j.1744-7909.2006.00169.x
    Osmotic stress caused by dehydration or high-salt conditions poses a major environmental challenge for plant growth and development. Many studies have shown that protein kinases are important regulators during osmotic stress in plants. In the present study, we describe GmAAPK, a soybean (Glycine max (L.) Merr.) gene coding for a putative serine/threonine protein kinase (abscisic acid (ABA)-activated protein kinase (AAPK)). The cDNA for GmAAPK is 1 409 bp long and contains a single long open reading frame representing a complete coding region of 359 amino acids. GmAAPK was mapped onto the D1a+Q linkage group. Its transcripts are expressed in all tissues, but at high levels in the cotyledon. GmAAPK mRNA was induced by polyethylene glycol, ABA, Ca2+, and Na+, but not cold (4 °C) treatments in soybean leaves. The results suggest that GmAAPK may participate in the regulatory process during osmotic stresses in soybean.(Author for correspondence.Tel: 010 6488 6859; Fax: 010 6487 3428; and
Abstract (Browse 2753)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
Identification of Festuca arundinacea Schreb Cat1 Catalase Gene and Analysis of its Expression Under Abiotic Stresses
Author: Wen-Long Yang, Jing-Mei Liu, Fan Chen, Qiang Liu, Yan-Dao Gong and Nan-Ming Zhao
Journal of Integrative Plant Biology 2006 48(3)
DOI: 10.1111/j.1744-7909.2006.00233.x
    Abiotic stresses, such as drought, high salinity, and cold/freezing, lead plants to produce excess reactive oxygen species. Catalase, a unique hydrogen peroxide-scavenging enzyme, plays a very important role in plants. To characterize the catalase involved in plant response to abiotic stresses, we constructed a cDNA library from 4 °C-treated Festuca arundinacea Schreb seedlings and isolated a catalase gene from this library. The cDNA (FaCat1, 1 735 bp) contained an open reading frame of 1 479 bp. BLAST analysis indicated that the deduced amino acid sequence showed 96% identity with that from wheat TaCat1 and 87% identity with that from maize ZmCat2. Northern blotting analysis showed an obvious increase of FaCat1 transcripts in leaves in contrast with roots. Time-course analysis of the expression of FaCat1 in F. arundinacea leaves showed that FaCat1 expression was upregulated in cold- and salt-stressed leaves, with the FaCat1 transcripts accumulating mostly at 4 or 2 h after cold or salt stress, respectively. No significant changes in FaCat1 transcription were observed in dried leaves and inhibition of FaCat1 transcription was found in abscisic acid (ABA)-treated leaves, indicating that the FaCat1 gene is differentially expressed during cold, high salt, drought, and ABA treatment in F. arundinacea leaves.作者联系方式:Tel: 010 6278 3261; Fax: 010 6279 4214; E-mail:
Abstract (Browse 3198)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
Genetic Analysis and Gene-Mapping of Two Reduced-Culm-Number Mutants in Rice
Author: Hua Jiang, Long-Biao Guo, Da-Wei Xue, Da-Li Zeng, Guang-Heng Zhang, Guo-Jun Dong, Ming-Hong Gu and Qian Qian
Journal of Integrative Plant Biology 2006 48(3)
DOI: 10.1111/j.1744-7909.2006.00224.x
    In the present study, in order to systematically dissect the genetic mechanism of rice (Oryza sativa L.) tilling for the super rice ideotype and the model system of branching development, two ethyl methane sulfonate-induced rice reduced-culm-number (rcn) mutants from the progeny of Nippobare (O. sativa ssp. japonica), namely rcn8 and rcn9, were used. Their maximum tillers were both less than 4. In addition, rcn9 had another major feature of rust-spotted leaves. Allelic tests between these two mutants and seven other recessive few-tiller mutants revealed that they were previously unknown loci. Genetic analysis showed that the rcn traits were all controlled by a pair of different recessive genes, designated as RCN8 and RCN9, respectively. Two F2 populations derived from crosses between the rcn8 or rcn9 mutants and 93-11 were constructed. Linkage analysis using two rcn F2 mapping populations with published simple sequence repeat markers demonstrated that the RCN8 and RCN9 genes were mapped on the long arm of chromosome 1 (119.6 cM) and the short arm of chromosome 6 (63.6 cM), respectively. The results of the present study are beneficial to map-based cloning and functional analysis of the RCN8 and RCN9 genes.(Author for correspondence.Tel: 0571 6337 0537; Fax: 0571 6337 0389;
Abstract (Browse 3365)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
Identification and Preliminary Analysis of Several Centromere-associated Bacterial Artificial Chromosome Clones from a Diploid Wheat Library  
Author: Zhao Liu, Wei Yue, Yu-Shen Dong and Xue-Yong Zhang
Journal of Integrative Plant Biology 2006 48(3): 348-363
DOI: 10.1111/j.1744-7909.2006.00223.x
    Although the centromeres of some plants have been investigated previously, our knowledge of the wheat centromere is still very limited. To understand the structure and function of the wheat centromere, we used two centromeric repeats (RCS1 and CCS1-5ab) to obtain some centromere-associated bacterial artificial chromosome (BAC) clones in 32 RCS1-related BAC clones that had been screened out from a diploid wheat (Triticum boeoticum Boiss.; 2n=2x=14) BAC library. Southern hybridization results indicated that, of the 32 candidates, there were 28 RCS1-positive clones. Based on gel blot patterns, the frequency of RCS1 was approximately one copy every 69.4 kb in these 28 RCS1-positive BAC clones. More bands were detected when the same filter was probed with CCS1-5ab. Furthermore, the CCS1 bands covered all the bands detected by RCS1, which suggests that some CCS1 repeats were distributed together with RCS1. The frequency of CCS1 families was once every 35.8 kb, nearly twice that of RCS1. Fluorescence in situ hybridization (FISH) analysis indicated that the five BAC clones containing RCS1 and CCS1 sequences all detected signals at the centromeric regions in hexaploid wheat, but the signal intensities on the A-genome chromosomes were stronger than those on the B- and/or D-genome chromosomes. The FISH analysis among nine Triticeae cereals indicated that there were A-genome-specific (or rich) sequences dispersing on chromosome arms in the BAC clone TbBAC5. In addition, at the interphase cells, the centromeres of diploid species usually clustered at one pole and formed a ring-like allocation in the period before metaphase.(Author for correspondence.Fax: 010 6213 5294; E-mail:
Abstract (Browse 3346)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
A Novel Flavonoid Glucoside from Anoectochilus roxburghii (Wall.) Lindl.
Author: Chun-Nian He, Chun-Lan Wang, Shun-Xing Guo, Jun-Shan Yang and Pei-Gen Xiao
Journal of Integrative Plant Biology 2006 48(3)
DOI: 10.1111/j.1744-7909.2006.00179.x
    Eight compounds were isolated from the ethyl acetate- and n-butanol-soluble fractions of the ethanolic extract of the whole plant of Anoectochilus roxburghii (Wall.) Lindl. (Orchidaceae). On the basis of spectroscopic methods, the structures of these compounds were elucidated as quercetin-7-O-β-D-[6''''-O-(trans-feruloyl)]-glucopyranoside (compound 1), 8-C-p-Hydroxybenzylquercetin (compound 2), isorhamnetin-7-O-β-D-glucopyranoside (compound 3), isorhamnetin-3-O-β-D-glucopyranoside (compound 4), kaempferol-3-O-β-D-glucopyranoside (compound 5), kaempferol-7-O-β-D-glucopyranoside (compound 6), 5-hydroxy-3'',4'',7-trimethoxyflavonol-3-O-β-D-rutinoside (compound 7), and isorhamnetin-3-O-β-D-rutinoside (compound 8). Of the compounds isolated, compound 1 was a new flavonoid glucoside and exhibited strong scavenging activity against the 1,1-diphenyl-2-picrylhydrazyl free radical, whereas the ethanolic extract showed weak activity. Compounds 2–8 were obtained from this family for the first time.(Author for correspondence.Tel: 010 6282 9619; Fax: 010 6289 5120; E-mail:
Abstract (Browse 3782)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
Norditerpenoid Alkaloids from Aconitum spicatum Stapf
Author: Li-Ming Gao, Hai-Yan Yan, Yang-Qing He and Xiao-Mei Wei
Journal of Integrative Plant Biology 2006 48(3)
DOI: 10.1111/j.1744-7909.2006.00178.x
    To search for pharmacologically and structurally interesting substances from traditional Chinese medicines, we investigated the chemical compounds of Aconitum spicatum Stapf. Two new norditerpenoid alkaloids, namely spicatine A (compound 1) and spicatine B (compound 2), as well as 11 known norditerpenoid alkaloids were isolated from the CHCl3 portion of the 90% ethanol extract of the roots of A. spicatum. The structures of the alkaloids were characterized on the basis of their spectral data. One of the isolated compounds showed significant cytotoxic activities (IC50 values < 200 μmol/L) against the HL-60 cell line.(Author for correspondence.Tel: 0931 797 1396; Fax: 0931 797 1989;
Abstract (Browse 2916)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
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