April 2006, Volume 48 Issue 4, Pages 377-496.

Cover Caption:
AO stained apoptotic nuclei of tobacco protoplasts.
Apoptotic nuclei show as bright orange fluorescence. Further apoptosis, shrinkage of chromatin and nuclear apoptotic bodies become prominent. See pages 433-439 for more details.


          Research Articles
Walnut Staminate Flowers Can Be Explored as a Supplementary Plant Oil Source
Author: Husen Jia and Yinong Xu
Journal of Integrative Plant Biology 2006 48(4)
DOI: 10.1111/j.1744-7909.2006.00191.x
    Fossil fuel is currently the major energy source driving global socio-economy, but its stock is being heavily depleted due to increasing anthropogenic activities worldwide. There are also concerns regarding the burning of fossil fuels, which contributes to global climate warming and air pollution. As such, the development of biodiesel as a non-toxic, biodegradable, and renewable alternative energy source using oil crops such as soybean and rapeseed has quickly emerged in the West countries. However, the production of oil crops in China is far from sufficient to meet the demands of the country¡¯s population of 1.3 billion, and increasing oil crop production is inhibited by a severe shortage of agricultural land, which currently averages 0.2 acre per person and, as such, is less than half the world average. The current national policy in China regarding land use is more towards revering cultivated lands in ravins and hills to forestry, which presents an ideal opportunity to further develop plantations of walnut (Juglans regia L.) trees, a plant that is tolerant to drought and infertile soils and has a high oil content. Study in this paper shows that one ament of walnut staminate flowers produces about 0.168 g dry pollen, and the dry pollen contained 49.67% oil. Based on this discovery, oil yield obtained from staminate flowers is estimated to reach 6.95% of that from walnut nuts. Thus walnut staminate flower is suggested to explore as supplementary plant oil source, and has a great opportunity to utilize as a biodiesel feedstock.(Author for correspondence.E-mail:yinongxu@ibcas.ac.cn)
Abstract (Browse 3060)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
The Cathaysia Flora and the Mixed Late Permian Cathaysian-Angaran Floras in East Asia
Author: Ke-Qin Sun
Journal of Integrative Plant Biology 2006 48(4)
DOI: 10.1111/j.1744-7909.2006.00207.x
    The Cathaysia flora, one of the most prominent floras of the Carboniferous and Permian, was mainly distributed in East Asia and was characterized by numerous endemic elements. China is one of the most important localities of the Cathaysia flora and it is also the center of origin of this flora. This paper reviews and discusses the characteristics of the Cathaysia flora and the biogeographically mixed Permian Cathaysian-Angaran floras of East Asia. In addition, the formative mechanism of the mixed Permian floras is also discussed.(Author for correspondence.Tel: +86(0)10 8232 0628; E-mail: keqins@cugb.edu.cn)
Abstract (Browse 2809)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
Achene Wall Anatomy and Surface Sculpturing of Lactuca L. and Related Genera (Compositae: Lactuceae) with Notes on Their Systematic Significance
Author: Shi-Xin Zhu, Hai-Ning Qin and Chu Shi
Journal of Integrative Plant Biology 2006 48(4)
DOI: 10.1111/j.1744-7909.2006.00245.x
    The achene wall anatomy and surface sculpturing of 14 species representing Lactuca L. and related genera were investigated to evaluate inter- and intrageneric relationships. The achene wall anatomy of the studied species can be divided into two types: winged and ribbed. The winged type is present in Lactuca L., Pterocypsela Shih, Cicerbita Wallr., Chaetoseris Shih and Stenoseris Shih, and is characterized by protruding wings and costae in transverse section. Winged type achene mesocarps are composed of parenchymatous cells and fiber cells, and the distinct fibrous strands are confined to the costae or wings of the achenes. The ribbed type is present in Notoseris Shih and Paraprenanthes Chang ex Shih, and is characterized by only having costae in transverse section. Fiber cells are continuous in the costae and intercostae. In surface sculpturing, the ornamentation and the shape of epidermal cells are different among these genera. The results indicate that L. altaica and L. serriola should be conspecific, and that L. dolichophylla is probably an intermediate taxon between Lactuca and Chaetoseris. The results also support the separation of Pterocypsela, Paraprenanthes, Notoseris, Chaetoseris and Stenoseris from Lactuca and Cicerbita as independent entities. Close affinities between Pterocypsela and Lactuca, Paraprenanthes and Notoseris, and Chaetoseris and Stenoseris are also proposed.(Author for correspondence.Tel: 010-62836023£» E-mail: hainingqin@ns.ibcas.ac.cn)
Abstract (Browse 3005)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
Clonal Plasticity in Response to Reciprocal Patchiness of Light and Nutrients in the Stoloniferous Herb Glechoma longituba L.
Author: Yu Chu, Fei-Hai Yu and Ming Dong
Journal of Integrative Plant Biology 2006 48(4)
DOI: 10.1111/j.1744-7909.2006.00237.x
    Interconnected ramets of clonal plants can functionally specialize in the uptake of resources from aboveground and/or underground sources. Ramet pairs of the clonal stoloniferous herb Glechoma longituba L. were grown in spatially heterogeneous environments with complementary availability of light and nutrients. Plasticity with respect to root-shoot ratio, fitness-related traits (biomass, number of ramets and dry weight per ramet), morphological traits (lamina area, root length) were experimentally examined. The aim was to understand the adaptation of G. longituba to an environment with reciprocal patchiness of light and soil nutrients by plasticity in its root-shoot ratio and clonal morphology. The results showed that the performance of ramets growing in patches with high light intensity and low soil nutrients into the adjacent opposite patches was increased in terms of fitness-related traits. However, the performance of those from patches with low light intensity and high soil nutrients into the adjacent opposite patches was not changed. The root-shoot ratio and clonal morphology were plastic. Morphological traits such as lamina area and root length were altered in a way that enhanced the capture of light resources and soil nutrients. Apparent reciprocal resource transport between the ramets in an environment of reciprocal patchiness of resources can enhance the growth of ramets with complementary resource deficiencies.(Author for correspondence.Tel(Fax): 010 8259 4676£» Email£ºdongming@ibcas.ac.cn)
Abstract (Browse 2755)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
Contribution of Root Respiration to Total Soil Respiration in a Leymus chinensis (Trin.) Tzvel. Grassland of Northeast China
Author: Wei Wang, Ji-Xun Guo, Jiang Feng and Takehisa Oikawa
Journal of Integrative Plant Biology 2006 48(4)
DOI: 10.1111/j.1744-7909.2006.00241.x
    The loss of carbon through root respiration is an important component of grassland carbon budgets. However, few data are available concerning the contribution of root respiration to total soil respiration in grasslands in China. We investigated seasonal variations of soil respiration rate, root biomass, microbial biomass C and organic C content of the soil in a semi-arid Leymus chinensis (Trin.) Tzvel. grassland of northeast China during the 2002 growing season (from May to September). The linear regression relationship between soil respiration rate and root biomass was used to determine the contribution of root respiration to total soil respiration. Soil respiration rate ranged from 2.5 to 11.9 g C/m2 per d with the maximum in late June and minimum in September. The microbial biomass C and organic C content of the soil ranged from 0.3 to 1.5 g C/m2 and from 29 to 34 g C/kg respectively. Root biomass had two peaks, in early June (1.80 kg/m2) and mid-August (1.73 kg/m2). Root respiration rate peaked in mid-August (6.26 g C/m2 per d), whereas microbial respiration rate peaked in late June (7.43 g C/m2 per d). We estimated that the contribution of root respiration to total soil respiration during the growing season ranged from 38% to 76%.(Author for correspondence.Tel: 010 6276 7922; Email:wendy_wangw@tom.com)
Abstract (Browse 2763)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
Effect of Water Deficit on Self-thinning Line in Spring Wheat (Triticum aestivum L.) Populations
Author: Jing Liu, Liang Wei, Chun-Ming Wang, Gen-Xuan Wang and Xiao-Ping Wei
Journal of Integrative Plant Biology 2006 48(4)
DOI: 10.1111/j.1744-7909.2006.00240.x
    Monocultures of spring wheat (Triticum aestivum L.) were grown at overcrowded densities (10 000 and 3 000 plants per m2) under well-watered and water-stressed conditions to investigate the effects of water deficits on self-thinning. The results showed that density reduction in water-stressed populations was delayed compared with that in well-watered populations. Populations grown in well-watered conditions conformed to the ¨C3/2-power law. Compared with the well-watered condition, there was no significant decrease of the self-thinning line under water-stressed conditions in this experiment, although the rate of average shoot biomass accumulation decreased. This result implied that the exponent of the ¨C3/2-power equation is not as sensitive as the rate of average shoot biomass accumulation to water stress. Further analysis indicated that, in each density treatment, the lines of the height versus shoot biomass relationships did not differ significantly between the two water conditions. However, the intercepts of the height versus shoot biomass relationships were greater in the higher-density populations (10 000/m2) than those in the lower-density populations (3 000/m2). These results showed that water deficit did not change plant geometry in this experiment. That is to say, shoot competition for light remains constant at a given biomass, although root competition for water becomes more serious in water deficit conditions. Based on these results and previous reports we propose that, to affect the thinning line slope, changes in symmetric competition are not as efficient as changes in asymmetric competition.(Author for correspondence.Tel(Fax): 0931 8912 893; Emai.:liuj219@163.com)
Abstract (Browse 2702)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
Anthocyanin Accumulation Mediated by Blue Light and Cytokinin in Arabidopsis Seedlings
Author: Da-Qing Chen Zhi-Yang Li Rui-Chi Pan and Xiao-Jing Wang
Journal of Integrative Plant Biology 2006 48(4)
DOI: 10.1111/j.1744-7909.2006.00234.x
    It has been reported that pigmentation in plants is stimulated by light and cytokinin (CTK); however, the signaling pathways and the relationship between light and CTK involved in the regulation of anthocyanin accumulation remain to be elucidated. We investigated (i) the role of blue light (BL) and CTK in anthocyanin accumulation ; and (ii) the relationship between BL and CTK in wild type (WT) and hy4 mutants of Arabidopsis thaliana. Two-d-old seedlings grown on medium with or without kinetin (KT) or zeatin (ZT) in darkness were irradiated using BL at different fluence rates for 3 d before the anthocyanin content was determined using a spectrophotometric method. Anthocyanin accumulation was strongly induced by BL in WT seedlings but not in hy4 seedlings, which demonstrated that CRY1 is the main photoreceptor for BL. Both KT and ZT enhanced the response of the WT seedlings to BL in a dose-dependent manner, whereas they were not sufficient to promote anthocyanin accumulation in darkness. In addition, data from experiments using the hy4 mutant showed that the CTK effect of BL was also CRY1-dependent. The results from experiments with three different treatment programs showed that the relationship between BL and KT in anthocyanin accumulation of Arabidopsis seedlings seems neither multiplicative nor additive coaction, but rather interaction. BL is necessary for anthocyanin accumulation, and KT might be involved in the BL signaling pathway.(Author for correspondence.Tel: +86 (0)20 8521 6417; Fax: +86 (0)20 8521 6417; E-mail:wangxj@scnu.edu.cn)
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Involvement of Hydrogen Peroxide Generated by Polyamine Oxidative Degradation in the Developmentof Lateral Roots in Soybean
Author: Guo-Xing Su, Wen-Hua Zhang and You-Liang Liu
Journal of Integrative Plant Biology 2006 48(4)
DOI: 10.1111/j.1744-7909.2006.00236.x
    In order to determine whether hydrogen peroxide (H2O2) generated by polyamine oxidative degradation is involved in the development of lateral roots in soybean, the length and the number of lateral roots, the activities of polyamine oxidases and diamine oxidases, and the endogenous free polyamine and H2O2 content were analyzed in soybean (Glycine max (Linn.) Merr.) main roots of 2-d-old seedlings after treatments for 2 d with exogenous ¦Â-hydroxyethylhydrazine (an inhibitor of polyamine oxidases), H2O2, putrescine, cyclohexylamine (an inhibitor of spermidine synthase) or N,N''-dimethylthiourea (a scavenger of hydrogen peroxide). ¦Â-hydroxyethylhydrazine treatment strongly inhibited the development of lateral roots in soybean seedlings, reduced the activities of polyamine oxidases and diamine oxidases, decreased H2O2 levels, and led to the accumulation of endogenous polyamines in the main roots. The inhibitory effect of ¦Â-hydroxyethylhydrazine on root development could be alleviated by exogenously applied 10 µmol/L H2O2 (a major product of polyamine oxidation). Treatment with cyclohexylamine and putrescine promoted root growth slightly, but treatment with cyclohexylamine plus N,N''-dimethylthiourea or putrescine plus N,N''-dimethylthiourea prevented the development of soybean lateral roots. The effects of these treatments on the development of soybean lateral roots were consistent with the changes in endogenous H2O2 levels. These results suggest that the development of soybean lateral roots is associated with the oxidative degradation of polyamines, and that their products, especially H2O2, are likely to play an important role in the growth of soybean lateral roots.(Author for correspondence.Tel: +86 (0)25 8439 5347; Fax: +86 (0)25 8439 6671; E-mail:liuyouliang @ njau.edu.cn¡¡or suguox017@yahoo.com.cn)
Abstract (Browse 2795)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
Calcium-Mediated Mitochondrial Permeability Transition Involved in Hydrogen Peroxide-Induced Apoptosis in Tobacco Protoplasts  
Author: Yuan Wang, Jiu-Sheng Lin and Gen-Xuan Wang
Journal of Integrative Plant Biology 2006 48(4): 433-439
DOI: 10.1111/j.1744-7909.2006.00219.x
    In the present study, we focused on whether intracellular free Ca2+ ([Ca2+]i) regulates the formation of mitochondrial permeability transition pore (MPTP) in H2O2-induced apoptosis in tobacco protoplasts. It was shown that the decrease in mitochondrial membrane potential (¡÷¦·m) preceded the appearance of H2O2-induced apoptosis; pretreatment with the specific MPTP inhibitor cyclosporine A, which also inhibits Ca2+ cycling by the mitochondria, effectively retarded apoptosis and the decrease in ¡÷¦·m. Apoptosis and decreased ¡÷¦·m were exacerbated by CaCl2, whereas the plasma membrane voltage-dependent Ca2+ channel blocker lanthanum chloride (LaCl3) attentuated these responses. Chelation of extracellular Ca2+ with EGTA almost totally inhibited apoptosis and the decrease in ¡÷¦·m induced by H2O2. The time-course of changes in [Ca2+]i in apoptosis was detected using the Ca2+ probe Fluo-3 AM. These studies showed that [Ca2+]i was increased at the very early stage of H2O2-induced apoptosis. The EGTA evidently inhibited the increase in [Ca2+]i induced by H2O2, whereas it was only partially inhibited by LaCl3. The results suggest that H2O2 may elevate cytoplasmic free Ca2+ concentrations in tobacco protoplasts, which mainly results from the entry of extracellular Ca2+, to regulate mitochondrial permeability transition. The signaling pathway of [Ca2+]i-mediated mitochondrial permeability transition was associated with H2O2-induced apoptosis in tobacco protoplasts.(Author for correspondence.Fax£º0931 891 2893£»Email£ºwanggx@lzu.edu.cn)
Abstract (Browse 2980)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
Genotyping the Heading Date of Male-Sterile Rice Line II-32A
Author: Jun-Feng Xu, Ling Jiang, Xiang-Jin Wei, Wei-Wen Zhang, Shi-Jia Liu, Liang-Ming Chen, Chun-Ming Wang, Lin-Guang Luo and Jian-Min Wan
Journal of Integrative Plant Biology 2006 48(4)
DOI: 10.1111/j.1744-7909.2006.00247.x
    II-32A, an elite male-sterile line of rice (Oryza sativa L.), has been widely used for the production of hybrid rice seed in China. Heading date in most combinations using II-32A shows transgressive inheritance or similarity to the latter parent, but the genotype of II-32A with respect to major genes for heading time is unknown. This limits the further exploitation of this sterile line in breeding and hybrid seed production. Using a number of major gene heading date isogenic lines and heading date QTL near-isogenic lines, we genetically analyzed II-32B under both long- and short-day conditions. We show that II-32B carries two photoperiod-sensitive genes, E1 and E3, a recessive late-heading gene, ef-1, and a photoperiod-sensitive allele, Se-1u. In addition we identified in II-32B a recessive inhibitor for E1 or Se-1n and other modified photoperiod-sensitive genes. The heading-date constitution of II-32A was determined to be E1e2E3Se-1uef-1i-Se-1.(Author for correspondence.E-mail: wanjm@njau.edu.cn)
Abstract (Browse 2869)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
Genetic Analysis and Mapping of the Dominant Dwarfing Gene D-53 in Rice  
Author: Li-Rong Wei, Ji-Chen Xu, Xiao-Bo Li, Qian Qian and Li-Huang Zhu
Journal of Integrative Plant Biology 2006 48(4): 447-452
DOI: 10.1111/j.1744-7909.2006.00248.x
    The dwarfing gene D-53 is one of a few dominant genes for dwarfing in rice (Oryza sativa L.). In the present study, our genetic analysis confirmed that mutant characteristics including dwarfing, profuse tillering, thin stems and small panicles are all controlled by the dominant D-53 gene. We measured the length of each internode of KL908, a D-53-carrying line, and classified the dwarfism of KL908 into the dn-type. In addition, we measured elongation of the second sheath and -amylase activity in the endosperm, and we characterized KL908 as a dwarf mutant that was neither gibberellic acid-deficient nor gibberellic acid-insensitive. Using a large F2 population obtained by crossing KL908 with a wild-type variety, NJ6, the D-53 gene was mapped to the terminal region of the short arm of chromosome 11, with one simple sequence repeat marker, Ds3, co-segregating, and the other, K81114, located 0.6 cM away.(Author for correspondence.Tel:010 6487 0491; Fax: 010 6487 3428£» E-mail£ºlhzhu@genetics.ac.cn)
Abstract (Browse 3209)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
Expression of the Grifola frondosa Trehalose Synthase Gene and Improvement of Drought-Tolerance in Sugarcane (Saccharum officinarum L.)
Author: Shu-Zhen Zhang, Ben-Peng Yang, Cui-Lian Feng, Ru-Kai Chen, Jing-Ping Luo, Wen-Wei Cai and Fei-Hu Liu
Journal of Integrative Plant Biology 2006 48(4)
DOI: 10.1111/j.1744-7909.2006.00246.x
    Trehalose is a nonreducing disaccharide of glucose that functions as a protectant in the stabilization of biological structures and enhances stress tolerance to abiotic stresses in organisms. We report here the expression of a Grifola frondosa trehalose synthase (TSase) gene for improving drought tolerance in sugarcane (Saccharum officinarum L.). The expression of the transgene was under the control of two tandem copies of the CaMV35S promoter and transferred into sugarcane by Agrobacterium tumefaciens EHA105. The transgenic plants accumulated high levels of trehalose, up to 8.805¨C12.863 mg/g fresh weight, whereas it was present at undetectable level in nontransgenic plants. It has been reported that transgenic plants transformed with Escherichia coli TPS (trehalose-6-phosphatesynthase) and/or TPP (trehalose-6-phosphate phosphatase) are severely stunted and have root morphologic alterations. Interestingly, our transgenic sugarcane plants had no obvious morphological changes and no growth inhibition in the field. Trehalose accumulation in 35S-35S:TSase plants resulted in increased drought tolerance, as shown by the drought and the drought physiological indexes, such as the rate of bound water/free water, plasma membrane permeability, malondialdehyde content, chlorophyll a and b contents, and activity of SOD and POD of the excised leaves. These results suggest that transgenic plants transformed with the TSase gene can accumulate high levels of trehalose and have enhanced tolerance to drought.(Author for correspondence.Tel: 0898 66892735£»E-mail: zhangsz@public.hk.hi.cn)
Abstract (Browse 3018)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
Diversity Suppression-Subtractive Hybridization Array for Profiling Genomic DNA Polymorphisms
Author: Tong-Xiang Li, Jin-Ke Wang, Yun-Fei Bai and Zu-Hong Lu
Journal of Integrative Plant Biology 2006 48(4)
DOI: 10.1111/j.1744-7909.2006.00249.x
    Genomic DNA polymorphisms are very useful for tracing genetic traits and studying biological diversity among species. Here, we present a method we call the ¡°diversity suppression-subtractive hybridization array¡± for effectively profiling genomic DNA polymorphisms. The method first obtains the subtracted gDNA fragments between any two species by suppression subtraction hybridization (SSH) to establish a subtracted gDNA library, from which diversity SSH arrays are created with the selected subtracted clones. The diversity SSH array hybridizes with the DIG-labeled genomic DNA of the organism to be assayed. Six closely related Dendrobium species were studied as model samples. Four Dendrobium species as testers were used to perform SSH. A total of 617 subtracted positive clones were obtained from four Dendrobium species, and the average ratio of positive clones was 80.3%. We demonstrated that the average percentage of polymorphic fragments of pairwise comparisons of four Dendrobium species was up to 42.4%. A dendrogram of the relatedness of six Dendrobium species was produced according to their polymorphic profiles. The results revealed that the diversity SSH array is a highly effective platform for profiling genomic DNA polymorphisms and dendrograms.(Author for correspondenceTel: 025 8361 9983£»Fax: 025 8361 9983£»E-mail: zhlu@seu.edu.cn.)
Abstract (Browse 3052)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
PCR-RFLP and AP-PCR of rbcL and ITS of rDNA Show That ¡ÁTaxodiomeria peizhongii (Taxodium ¡Á Cryptomeria) Is not an Intergeneric Hybrid
Author: Yan Ling, Wei-Feng Lu, Fan Lu, Yu-Guo Wang, Jia-Kuan Chen and Wen-Ju Zhang
Journal of Integrative Plant Biology 2006 48(4)
DOI: 10.1111/j.1744-7909.2006.00209.x
    ¡ÁTaxodiomeria peizhongii Z. J. Ye, J. J. Zhang et S. H. Pan was regarded as a new intergeneric hybrid between Taxodium mucronatum Tenore (as the female donor) and Cryptomeria fortunei Hooibrenk ex Otto et Dietr (as the male donor). To confirm the authenticity of the intergeneric hybrid, we analyzed the rbcL gene and the internal transcribed spacer (ITS) of 26S¨C18S ribosomal RNA gene of the three species using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and arbitrarily primed PCR (AP-PCR), and obtained the following results: i) Taxodiomeria peizhongii had the same RFLP maps of the rbcL gene and the ITS as Taxodium mucronatum, but was different from C. fortunei; ii) a 311-bp PCR amplification product was obtained in C. fortunei by AP-PCR of ITS, but was not found in Taxodiomeria peizhongii. Our results have demonstrated that C. fortunei did not provide any genome for Taxodiomeria peizhongii, implying that T. peizhongii is not an intergeneric hybrid between the two species.(Author for correspondence.Tel£º+86 (0) 21 6564 2819£»Fax: +86 (0) 21 6564 2468£»E-mail: wjzhang@fudan.edu.cn)
Abstract (Browse 3148)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
Two New Cytotoxic Sesquiterpenoids from Eupatorium lindleyanum DC.
Author: Jun Huo, Sheng-Ping Yang, Jian Ding and Jian-Min Yue
Journal of Integrative Plant Biology 2006 48(4)
DOI: 10.1111/j.1744-7909.2006.00226.x
    Two new sesquiterpenoid lactones, namely eupalinilides K and L, were isolated from Eupatorium lindleyanum DC. Their structures were determined by spectral methods, including 1D- and 2D-NMR spectra. Cytotoxic evaluation showed that eupalinilide L exhibited potent cytotoxicity against P-388 and A-549 tumor cell lines with IC50 values of 0.17 and 2.60 ¦Ìmol/L, respectively.(Author for correspondence.E-mail: jmyue@mail.shcnc.ac.cn)
Abstract (Browse 2545)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
Labdane Diterpenoids from Coleus forskohlii (Willd.) Briq.
Author: Ling-Ling Xu and Ling-Yi Kong
Journal of Integrative Plant Biology 2006 48(4)
DOI: 10.1111/j.1744-7909.2006.00177.x
    In order to investigate the active substances of Coleus forskohlii (Willd.) Briq., a new labdane diterpenoid, 12-hydroxy-8,13E-labdadien-15-oic acid, together with two 8,13-epoxylabd-14-ene-11-one diterpenoids, 6-acetyl-1-deoxyforskolin and 6-acetyl-1,9-dideoxyforskolin, was isolated on the basis of various chromatographic techniques and spectroscopic methods.(Author for correspondence.Tel: +86(0)25 8539 1289; Fax: +86(0)25 8530 1528; E-mail: lykong@jlonline.com)
Abstract (Browse 2757)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
Effects of Overexpression of the Endogenous Farnesyl Diphosphate Synthase on the Artemisinin Content in Artemisia annua L.
Author: Jun-Li Han, Ben-Ye Liu, He-Chun Ye, Hong Wang, Zhen-Qiu Li and Guo-Feng Li
Journal of Integrative Plant Biology 2006 48(4)
DOI: 10.1111/j.1744-7909.2006.00208.x
    Artemisinin is a novel effective antimalarial drug extracted from the medicinal plant Artemisia annua L. Owing to the tight market and low yield of artemisinin, there is great interest in enhancing the production of artemisinin. In the present study, farnesyl diphosphate synthase (FPS) was overexpressed in high-yield A. annua to increase the artemisinin content. The FPS activity in transgenic A. annua was two- to threefold greater than that in non-transgenic A. annua. The highest artemisinin content in transgenic A. annua was approximately 0.9% (dry weight), which was 34.4% higher than that in non-transgenic A. annua. The results demonstrate the regulatory role of FPS in artemisinin biosynthesis.(Author for correspondence.Tel:010-62836249; Fax:010-82591016; E-mail:hcye@ibcas.ac.cn)
Abstract (Browse 2926)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
Screening of Fungi from Chinese Medical Plants for Anti-Human Immunodeficiency Virus Type 1 Activity
Author: Zi-Chun Xiang, Yan Jiang and Shun-Xing Guo
Journal of Integrative Plant Biology 2006 48(4)
    In order to isolate anti-human immunodeficiency virus (HIV) agents from natural products, 97 ethanolic extracts of 90 fungi were tested for their inhibitory activity on HIV-1. Most of the extracts tested were relatively non-toxic to human lymphocytic MT-4 cells, but extracts of some fungi exhibited potent anti-HIV activity in an in vitro 3-(4,5-dimethyl-2 thiazoyl)-2,5-diphenyl-2H-tetrazolium bromide assay with a selectivity index greater than 3. Most fungi were isolated from Dendrobium sp. and Taxus sp.(Author for correspondence.Tel: +86 10 6302 4898; Fax: +86 10 6302 4898; E-mail: Jiangyan03@263.net)
Abstract (Browse 2428)       
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