August 2006, Volume 48 Issue 8, Pages 873-1000.


Cover Caption:
The complex of HTA and trypsin (left top). Model of the HTA-b monomer (top) and trypsin, residues 18每22 of HTA-b shown by a ball and stick model (bottom). Details of the interaction (right below). A Connolly surface diagram represents the active binding pocket of trypsin; a stick model represents only residues 18每22 of HTAb. See pages 971每982 for more details.

 

          Research Articles
Putative Nitrogen Sensing Systems in Higher Plants
Author: Hon-Ming Lam, Ying Ann Chiao, Man-Wah Li, Yuk-Kwong Yung and Sang Ji
Journal of Integrative Plant Biology 2006 48(8)
DOI: 10.1111/j.1744-7909.2006.00313.x
      
    Nitrogen (N) metabolism is essential for the biosynthesis of vital biomolecules. N status thus exerts profound effects on plant growth and development, and must be closely monitored. In bacteria and fungi, a few sophisticated N sensing systems have been extensively studied. In animals, the ability to receive amino acid signals has evolved to become an integral part of the nervous coordination system. In this review, we will summarize recent developments in the search for putative N sensing systems in higher plants based on homologous systems in bacteria, fungi, and animals. Apparently, although plants have separated and diversified from other organisms during the evolution process, striking similarities can be found in their N sensing systems compared with those of their counterparts; however, our understanding of these systems is still incomplete. Significant modifications of the N sensing systems (including cross-talk with other signal transduction pathways) in higher plants may be a strategy of adaptation to their unique mode of life.(Author for correspondence.Tel(Fax): +852 2609 6336; E-mail:honming@cuhk.edu.hk)
Abstract (Browse 2344)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
Effect of Seasonality on Distribution of Macroalgae in a Stream System (Xin*an Spring) in Shanxi Province, North China
Author: Bian-Fang Hu and Shu-Lian Xie
Journal of Integrative Plant Biology 2006 48(8)
DOI: 10.1111/j.1744-7909.2006.00317.x
      
    A survey of the seasonal distribution of macroalgae in a stream system in Shanxi Province, north China, was undertaken from July 2004 to April 2005. The relative abundance and percentage cover of macroalgae, and several environmental factors were monitored along a 20-m stretch at each of four sites, at intervals of approximately three months (one sampling per season). Several stream conditions were relatively constant over the sampling period (pH, maximum width and maximum depth), whereas others exhibited a distinct seasonal pattern (water temperature and specific conductance), and some fluctuated with no discernable seasonal pattern (current velocity and dissolved oxygen). Forty-two species of macroalgae were found, with a predominance of Chlorophyta (26 species, 61.9%). Rhodophyta and Charophyta represented the smallest proportion (1 species each, 2.38%). Six macroalgae species were the most widespread, occurring in all four sampling sites. Twelve species were found at only one site each. In terms of seasonality, eight species occurred throughout the year, whereas 16 species were found in only one season each. The macroalgal community at Xin''an Spring was species rich relative to other streams. Species richness per sampling site was negatively correlated with pH. Principal component analysis revealed that no single variable had much influence on the macroalgal seasonal dynamics. We calculated Sorensen similarity indices to compare our study with other continent-wide surveys of stream macroalgae, but the similarity indices were all very low. This study also shows that macroalgae in different locations have significant reproductive isolation.(Author for correspondence.Tel: +86 (0)351 701 8121; Fax: +86 (0)351 701 812; E-mail:xiesl@sxu.edu.cn)
Abstract (Browse 2234)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
Stipa tenacissima Does not Affect the Foliar 汛13C and 汛15N of Introduced Shrub Seedlings in a﹛Mediterranean Semi-arid Steppe
Author: Fernando T. Maestre, Susana Bautista and Jordi Cortina
Journal of Integrative Plant Biology 2006 48(8)
DOI: 10.1111/j.1744-7909.2006.00295.x
      
    Recent studies have shown that the tussock grass Stipa tenacissima L. facilitates the establishment of late-successional shrubs, in what constitutes the first documented case of facilitation of woody plants by grasses. With the aim of increasing our knowledge of this interaction, in the present study we investigated the effects of S. tenacissima on the foliar 汛13C, 汛15N, nitrogen concentration, and carbon : nitrogen ratio of introduced seedlings of Pistacia lentiscus L., Quercus coccifera L., and Medicago arborea L. in a semi-arid Mediterranean steppe. Six months after planting, the values of 汛13C ranged between 每26.9㏑ and 每29.6㏑, whereas those of 汛15N ranged between 每1.9㏑ and 2.7㏑. The foliar C : N ratio ranged between 10.7 and 53.5, and the nitrogen concentration ranged between 1.0% and 4.4%. We found no significant effect of the microsite provided by S. tenacissima on these variables in any of the species evaluated. The values of 汛13C were negatively correlated with predawn water potentials in M. arborea and were positively correlated with relative growth rate in Q. coccifera. The values of 汛15N were positively correlated with the biomass allocation to roots in the latter species. The present results suggest that the modification of environmental conditions in the are surrounding S. tenacissima was not strong enough to modify the foliar isotopic and nitrogen concentration of shrubs during the early stages after planting.(Author for correspondence.Tel: +94 91 488 8511; Fax: +94 91 664 7490; E-mail:fernando.maestre@urjc.es)
Abstract (Browse 2134)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
Different Patterns of Changes in the Dry Season Diameter at Breast Height of Dominant and Evergreen Tree Species in a Mature Subtropical Forest in South China
Author: Jun-Hua Yan, Guo-Yi Zhou, De-Qiang Zhang, Xu-Li Tang and Xu Wang
Journal of Integrative Plant Biology 2006 48(8)
DOI: 10.1111/j.1744-7909.2006.00273.x
      
    Information on changes in diameter at breast height (DBH) is important for net primary production (NPP) estimates, timing of forest inventory, and forest management. In the present study, patterns of DBH change were measured under field conditions during the dry season for three dominant and native tree species in a monsoon evergreen broad-leaved forest in the Dinghushan Biosphere Reserve. For each tree species, different patterns of DBH change were observed. In the case of the fast-growing tree species Castanopsis chinensis Hance, large diurnal fluctuations occur, with a peak DBH in the early morning (around 05:00 h) that decreases to a minimum by about 14:00 h. Both Schima superba Gardn. et Chemp and Cryptocarya chinensis (Hance) Hemsl. exhibited less diurnal swelling and shrinkage. Diurnal fluctuations for these species were observed on a few occasions over the period of observation. Graphical comparisons and statistical analysis of changes in DBH with meteorological variables indicate that for different trees, the different changes in DBH observed responded to different meteorological variables. Large stem changes were found to occur for Ca. chinensis trees that were associated with variations in solar radiation. However, both S. superba and Cr. chinensis were found to be less sensitive to solar radiation. Changes in the DBH of these two species were found to be controlled mainly by soil temperature and soil moisture. During the later dry season, with a lower soil temperature and soil moisture, all three tree species stopped growing and only negligible shrinkage, expansion, or fluctuation occurred, suggesting that the optimum time to measure tree growth in the Dinghushan Biosphere Reserve is the later dry season.(Author for correspondence.Tel: 020 37252720; Fax: 020 37252615; E-mail:jhyan@scib.ac.cn)
Abstract (Browse 2304)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
Effects of Salt Stress on Carbohydrate Metabolism in Desert Soil Alga Microcoleus vaginatus Gom.
Author: Lan-Zhou Chen, Dun-Hai Li, Li-Rong Song, Chun-Xiang Hu, Gao-Hong Wang﹛and Yong-Ding Liu
Journal of Integrative Plant Biology 2006 48(8)
DOI: 10.1111/j.1744-7909.2006.00291.x
      
    The effects of salt stress on carbohydrate metabolism in Microcoleus vaginatus Gom., a cyanobacterium isolated from desert algal crusts, were investigated in the present study. Extracellular total carbohydrates and exopolysaccharides (EPS) in the culture medium produced by M. vaginatus increased significantly during the growth phase and reached a maximum during the stationary phase. The production of extracellular carbohydrates also significantly increased under higher salt concentrations, which was attributed to an increase in low molecular weight carbohydrates. In the presence of NaCl, the production of cellular total carbohydrates decreased and photosynthetic activity was impaired, whereas cellular reducing sugars, water-soluble sugars and sucrose content and sucrose phosphate synthase activity increased, reaching a maximum in the presence of 200 mmol/L NaCl. These parameters were restored to original levels when the algae were transferred to a non-saline medium. Sodium and K+ concentrations of stressed cells decreased significantly and H+-ATPase activity increased after the addition of exogenous sucrose or EPS. The results suggest that EPS and sucrose are synthesized to maintain the cellular osmotic equilibrium between the intra- and extracellular environment, thus protecting algal cells from osmotic damage, which was attributed to the selective exclusion of cellular Na+ and K+ by H+-ATPase.(Author for correspondence.Tel(Fax): +86 (0) 27 6878 0036; E-mail:liuyd@ihb.ac.cn )
Abstract (Browse 2388)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
Effects of Exogenous Spermidine on Photosystem II of Wheat Seedlings Under Water Stress
Author: Hui-Guo Duan, Shu Yuan, Wen-Juan Liu, De-Hui Xi, Dong-Hong Qing,Hou-Guo Liang and Hong-Hui Lin
Journal of Integrative Plant Biology 2006 48(8)
DOI: 10.1111/j.1744-7909.2006.00315.x
      
    The effects of exogenous spermidine (Spd) on lipid peroxidation, relative plasma membrane permeability, photosystem II (PSII) gene expression and PSII photochemical activity in water-stressed wheat seedlings were investigated. The decrease in relative water content (RWC), Chl content, and 2,6-dichlorophenol indophenol (DCIP) photoreduction of PSII, and increases in electrolyte leakage of plasma membranes and malonyldialdehyde (MDA) in water-stressed leaves was alleviated by Spd pretreatment. Furthermore, Western and Northern blot analysis showed that decreases in the PSII major proteins D1, D2 and LHCII and the transcripts of corresponding genes psbA, psbD and cab were also alleviated by Spd pretreatment under water stress. These results suggest that the application of exogenous Spd protects PSII against water stress at both the transcriptional level and the translational level, and allows PSII to retain a higher activity level during water stress. The protective role of Spd in the photosynthetic apparatus also is discussed.(Author for correspondence.Telㄩ028 8541 1175; E-mail:honghuilin@hotmail.com)
Abstract (Browse 2311)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
Enhanced Cadmium Accumulation in Transgenic Tobacco Expressing the Phytochelatin Synthase Gene of Cynodon dactylon L.
Author: Jiangchuan Li, Jiangbo Guo, Wenzhong Xu and Mi Ma
Journal of Integrative Plant Biology 2006 48(8)
DOI: 10.1111/j.1744-7909.2006.00314.x
      
    Bermudagrass (Cynodon dactylon L. cv. Goldensun) is highly resistant to and accumulates large amounts of cadmium (Cd). A phytochelatin synthase (PCS) cDNA (CdPCS1) was isolated from this grass by rapid amplification of cDNA ends. The putative CdPCS1 protein shared a high homology with PCS from other plants, with 79% homology at the N-terminal and 47% homology at the C-terminal. However, 16 Cys residues were found at the C-terminal of CdPCS1, and among these residues, three positions were different from other PCS proteins. Semiquantitative reverse transcription-polymerase chain reaction analysis showed that Cd stress induced CdPCS1 expression in both roots and leaves in Bermudagrass. We verified that CdPCS1 plays an important role in Cd tolerance in yeast cells by expressing the gene in ABDE1, a Cd-sensitive mutant. CdPCS1 was then introduced into tobacco plants. The phytochelatin level in some transgenic tobacco lines increased 3.88-fold more than in wild type plants and Cd accumulation in these transgenic plants was enhanced 3.21-fold accordingly. The results suggested that CdPCS1 could be used as a gene element for phytoremediation in the future.(Author for correspondence. Tel: 86-10-62836255. E-mail: mami@ibcas.ac.cn)
Abstract (Browse 2457)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
Mapping Quantitative Trait Loci for Post-Anthesis Dry Matter Accumulation in Wheat  
Author: Jun-Ying Su, Yi-Ping Tong, Quan-You Liu, Bin Li, Rui-Lian Jing, Ji-Yun Li and Zhen-Sheng Li
Journal of Integrative Plant Biology 2006 48(8): 938-944
DOI: 10.1111/j.1744-7909.2006.00252.x
      
    Post-anthesis photoassimilation is very important for wheat (Triticum aestivum L.) grain filling. The aim of the present study was to map quantitative trait loci (QTL) for post-anthesis dry matter accumulation (DMA). A set of 120 doubled haploid (DH) lines, derived from winter wheat varieties Hanxuan 10 and Lumai 14, was grown under field conditions in two consecutive growing seasons during 2002每2004 in Beijing. Post-anthesis DMA per culm and related traits, including flag leaf greenness (FLG) and flag leaf weight (FLW; dry weight per flag leaf) at flowering, and grain weight per ear (GWE) were investigated. All traits segregated continuously in the DH population in both trials. The DMA was significantly and positively correlated with GWE, with the correlation coefficients being 0.79 and 0.66 in the 2002每2003 and 2003每2004 growing seasons (both P < 0.01), suggesting the importance of DMA in grain filling. Further correlation analysis showed that FLW was more closely correlated with DMA and GWE than FLG in both growing seasons, indicating that FLW was more important than FLG in influencing DMA and GWE. In total, 30 QTLs for these four traits were mapped and distributed on 10 chromosomes. Phenotypic variations explained by an individual QTL were in the range 5.8%每21.3%, 5.9%每17.2%, 5.1%每18.1%, and 5.6%每16.2% for FLG, FLW, DMA, and GWE, respectively. Eight QTLs for DMA were detected, of which four (on chromosome arms 2AS, 4BL, 5AS, and 7AS) were linked with QTLs for GWE; two (on chromosome arms 5BL and 7BL) coincided with QTLs for FLW. These results may provide useful information for developing marker-assisted selection for the improvement of DMA.(Author for correspondence.Telㄩ+86 (0) 10 6488 9381˙Faxㄩ+86 (0) 10 6488 9381˙E-mail:yptong@genetics.ac.cn)
Abstract (Browse 3499)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
Proteomic Analysis of the Response of Liangyoupeijiu (Super High-Yield Hybrid Rice) Seedlings to Cold Stress
Author: Ping-Fang Yang, Xiao-Juan Li, Yu Liang, Yu-Xiang Jing, Shi-Hua Shen and Ting-Yun Kuang
Journal of Integrative Plant Biology 2006 48(8)
DOI: 10.1111/j.1744-7909.2006.00319.x
      
    Liangyoupeijiu is a super high-yield hybrid rice. Despite its advantages with respect to yield and grain quality, it is sensitive to cold, which keeps it from being widely cultivated. We subjected Liangyoupeijiu seedlings to 4 ∼C cold treatment, then extracted the leaf proteins. After 2-D gel electrophoresis separation and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis, a series of differentially displayed proteins were identified. Some metabolism-associated proteins were found among the downregulated proteins, such as carbamoyl phosphate synthetase, transketolase 1, dihydrolipoamide dehydrogenase and glyceraldehyde 3-phosphate dehydrogenase. The upregulated proteins included both stress-resistance proteins such as nucleoside diphosphate kinase I and proteins that are negative for rice growth, such as FtsH-like protein, plastid fusion and/or translocation factor (Pftf) and actin. Our results indicate that cold may inhibit Liangyoupeijiu growth through decreasing metabolic activity and damaging cell structure.(Author for correspondence.Tel:(0)10 6259 6545˙Fax: (0)10 6259 6594E-mail:shshen@ibcas.ac.cn)
Abstract (Browse 2244)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
Cloning of Salt Tolerance-Related cDNAs from the Mangrove Plant Sesuvium portulacastrum L.
Author: Hui-Cai Zeng, Liu-Hong Deng and Chun-Fa Zhang
Journal of Integrative Plant Biology 2006 48(8)
DOI: 10.1111/j.1744-7909.2006.00287.x
      
    In an attempt to isolate and identify the target genes relevant to salt tolerance in a mangrove plant (Sesuvium portulacastrum L.), a subtracted cDNA library was constructed via suppressive subtractive hybridization (SSH), in which the poly(A)+RNA isolated from salt-tolerant S. portulacastrum leaves was used as a tester, whereas the driver was poly(A)+RNA, derived from salt-sensitive S. portulacastrum leaves. Screening of this subtracted cDNA library revealed five clones, of which the expression levels in the salt-tolerant plant were markedly higher than those observed in the salt-sensitive plant, indicating that these candidate clones may be involved in salt-tolerance pathways. Among the clones isolated, P66, P175, and P233 are novel because no significant similarity was obtained upon alignment with the GenBank database. Clone P89 demonstrated high homology with NADPH of Arabidopsis thaliana, whereas clone P152 was highly homologous with the gene encoding late embryogenesis abundant (LEA) protein of A. thaliana. The full-length gene of clone P152, with a predicated 344 amino acid residues, was shown to bear LEA-2 domains, a signature motif for proteins that have been enriched under salty and drought conditions. It is thus implied that clone P152 would be a salt-tolerance gene of S. portulacastrum. In addition, we have also developed a strategy for the extraction of total RNA from mangrove plants.(Author for correspondence.Tel: 0898 6689 2946˙Fax: 0898 6689 0987˙E-mail:zhang_cs@hotmail.com)
Abstract (Browse 2346)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
Characterization of Wheat Random Amplified Polymorphic DNA Markers Associated with the H11 Hessian Fly Resistance Gene
Author: Dhia Bouktila, Maha Mezghani, Mohamed Marrakchi and Hanem Makni
Journal of Integrative Plant Biology 2006 48(8)
DOI: 10.1111/j.1744-7909.2006.00254.x
      
    In Tunisia, the Hessian fly Mayetiola destructor Say is a major pest of durum wheat (Triticum durum Desf.) and bread wheat (T. aestivum L.). Genetic resistance is the most efficient and economical method of control of this pest. To date, 31 resistance genes, designated H1每H31, have been identified in wheat. These genes condition resistance to the insect genes responsible for virulence. Using wheat cultivars differing for the presence of an individual Hessian fly resistance gene and random amplified polymorphic DNA (RAPD) analysis, we have identified a polymorphic 386-bp DNA marker (Xgmib1-1A.1) associated with the H11 Hessian fly resistance gene. Blast analysis showed a high identity with a short region in the wild wheat (T. monococcum) genome, adjacent to the leaf rust resistance Lr10 gene. A genetic linkage was reported between this gene (Lr10) and Hessian fly response in wheat. These data were used for screening Hessian fly resistance in Tunisian wheat germplasm. Xgmib1-1A.1-like fragments were detected in four Tunisian durum and bread wheat varieties. Using these varieties in Hessian fly breeding programs in Tunisia would be of benefit in reducing the damage caused by this fly.(Author for correspondence.Tel: +216 2256 9664; Fax: +216 7086 0432; E-mail:dhia_bouktila2000@yahoo.fr)
Abstract (Browse 2280)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
Proteomic Alterations of Antarctic Ice Microalga Chlamydomonas sp. Under Low-Temperature Stress
Author: Guang-Feng Kan, Jin-Lai Miao, Cui-Juan Shi and Guang-You Li
Journal of Integrative Plant Biology 2006 48(8)
DOI: 10.1111/j.1744-7909.2006.00255.x
      
    Antarctic ice microalga can survive and thrive in cold channels or pores in the Antarctic ice layer. In order to understand the adaptive mechanisms to low temperature, in the present study we compared two-dimensional polyacrylamide gel electrophoresis (2-DE) profiles of normal and low temperature-stressed Antarctic ice microalga Chlamydomonas sp. cells. In addition, new protein spots induced by low temperature were identified with peptide mass fingerprinting based on matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and database searching. Well-resolved and reproducible 2-DE patterns of both normal and low temperature-stressed cells were acquired. A total of 626 spots was detected in control cells and 652 spots were detected in the corresponding low temperature-stressed cells. A total of 598 spots was matched between normal and stressed cells. Two newly synthesized proteins (a and b) in low temperature-stressed cells were characterized. Protein spot A (53 kDa, pI 6.0) was similar to isopropylmalate/homocitrate/citramalate synthases, which act in the transport and metabolism of amino acids. Protein spot b (25 kDa, pI 8.0) was related to glutathione S-transferase, which functions as a scavenger of active oxygen, free radicals, and noxious metabolites. The present study is valuable for the application of ice microalgae, establishing an ice microalga Chlamydomonas sp. proteome database, and screening molecular biomarkers for further studies.(Author for correspondence.Telㄩ(0)631 568 7759ㄛ E-mailㄩkanguangfeng@163.com)
Abstract (Browse 2280)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
Cloning and Functional Analysis of the Bifunctional Agglutinin/Trypsin Inhibitor from Helianthus tuberosus L.  
Author: Tuanjie Chang, Hongli Zhai, Songbiao Chen, Guisheng Song, Honglin Xu,Xiaoli Wei and Zhen Zhu
Journal of Integrative Plant Biology 2006 48(8): 971-982
DOI: 10.1111/j.1744-7909.2006.00321.x
      
    In order to find new insect resistance genes, four homologous cDNAs, hta-a, hta-b, hta-c and hta-d with lengths of 775, 718, 784 and 752 bp, respectively (GenBank accession numbers AF477031每AF477034), were isolated from a tuber cDNA expression library of Helianthus tuberosus L. Sequence analysis revealed that all four cDNAs contain an open reading frame of 444 bp, coding a polypeptide of 147 amino acid residues, and that the sequences of the cDNAs are very similar to those of the mannose-binding agglutinin genes of the jacalin-related family. In hemagglutination reactions and hapten inhibition assays, affinity-purified HTA(Helianthus tuberosus agglutinin) from induced Escherichia coli BL21(DE3) expressing GST-HTA shows hemagglutination ability and a higher carbohydrate-binding ability for mannose than other tested sugars. Trypsin inhibitory activity was detected in the crude extracts of induced E. coli BL21(DE3) expressing HTA, and was further verified by trypsin inhibitory activity staining on native polyacrylamide gel. The mechanism of interaction between HTA and trypsin was studied by molecular modeling. We found that plenty of hydrogen bonds and electrostatic interactions can be formed between the supposed binding sites of HTA-b and the active site of trypsin, and that a stable HTA/trypsin complex can be formed. The results above imply that HTA might be a bifunctional protein with carbohydrate-binding activity and trypsin inhibitory activity. Moreover, Northern blotting analysis demonstrated that hta is predominantly expressed in tubers of H. tuberosus, very weakly expressed in stems, but not expressed at all in other tissues. Southern blotting analysis indicated that hta is encoded by a multi-gene family. The insect resistance traits have been described in another paper.(Author for correspondence.)Tel(Fax): (0)10 6485 2890˙E-mail:zzhu@genetics.ac.cn
Abstract (Browse 2560)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
Palaeophytochemical Constituents of Cretaceous Ginkgo coriacea Florin Leaves
Author: You-Xing Zhao, Cheng-Sen Li, Xiao-Dong Luo, Yu-Fei Wang and Jun Zhou
Journal of Integrative Plant Biology 2006 48(8)
DOI: 10.1111/j.1744-7909.2006.00283.x
      
    Chemical investigation of the organic solvent extract of Cretaceous Ginkgo coriacea Florin leaves by liquid chromatography-mass spectroscopy (LC-MS) and gas chromatography-mass spectrometry (GC-MS), analogous to those from extant leaves of Ginkgo biloba L., led to the detection of a group of natural flavonoids and other volatiles. The similarity of the chemical constituents in these two species of Ginkgo suggest that the secondary metabolism of extant G. biloba is close to that of the Cretaceous species. The remaining natural products may be one explanation why the leaves of the Cretaceous G. coriacea have been preserved morphologically in fossilization. The detection of flavonoids suggests that the leaves of G. coriacea experienced a mild post-depositional environment during their fossilization. This appears to be the oldest occurrence of flavonoids in plant fossils.(Author for correspondence.Tel: (0)871 522 3264; Fax: (0)871 522 3261; E-mail:jzhou@mail.kib.ac.cn)
Abstract (Browse 2312)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
Chemical Constituents of Siegesbeckia orientalis L.
Author: Li-Li Wang and Li-Hong Hu
Journal of Integrative Plant Biology 2006 48(8)
DOI: 10.1111/j.1744-7909.2006.00279.x
      
    The aerial parts of Siegesbeckia have been used as the traditional Chinese medicine in the treatment of rheumatic arthritis, hypertension, malaria, neurasthenia, and snakebite. In order to find new and bioactive compounds, the chemical constituents of the aerial parts of S. orientalis L. were investigated and two new compounds, namely 汕-D-glucopyranosyl-ent-2-oxo-15,16-dihydroxy-pimar-8(14)-en-19-oic-late (compound 1) and [1(10)E,4Z]-8汕-angeloyloxy-9汐-methoxy-6汐,15-dihydroxy-14-oxogermacra-1(10),4,11(13)-trien-12-oic acid 12,6-lactone (compound 2), as well as five known ent-pimarane diterpenes (compounds 3每7) were isolated. The structures of the two new compounds were identified by their physicochemical properties and spectral analysis, particularly one- and two-dimensional nuclear magnetic resonance spectral methods.(Author for correspondence.Tel: 021-50272221; Fax: 021-50272221; E 每mail:simmhulh@mail.shcnc.ac.cn)
Abstract (Browse 2524)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
Water-Soluble Constituents of Cudrania tricuspidata (Carr.) Bur.
Author: Zong-Ping Zheng, Jing-Yu Liang and Li-Hong Hu
Journal of Integrative Plant Biology 2006 48(8)
DOI: 10.1111/j.1744-7909.2006.00227.x
      
    In order to find new structural and biologically active compounds, the constituents of the bark of Cudrania tricuspidata (Carr.) Bur. were investigated and a new 6-p-hydroxybenzyltaxifolin glucoside, named tricusposide (compound 1), together with 16 known compounds, was isolated by solvent partition, macroporous adsorption resin AB-8, silica gel, Sephadex LH-20 chromatography. Using spectroscopic methods, the structures of the compounds were elucidated as 6-p-hydroxybenzyl taxifolin-7-O-汕-D-glucoside (compound 1), dihydroquerctin-7-O-汕-D-glucoside (compound 2), dihydrokaempferol-3-O-汕-D-glucoside (compound 3), dihydroquercetin (compound 4), peonoside (compound 5), sphaerobioside (compound 6), quercimeritrin (compound 7), genistein (compound 8), aromadendrin (compound 9), kaempferol (compound 10), genistin (compound 11), 3,4-dihydroxystyryl alcohol (compound 12), sucrose (compound 13), 1,3,5,6-tetrahydroxyxanthone (compound 14), gericudranin E (compound 15), gericudranin C (compound 16), and orobol (compound 17). Compounds 2每6, 8, 9, 12每14, and 17 were isolated from this genus for the first time.(Author for correspondence.TelㄗFaxㄘㄩ+86 (0) 21 5027 2221˙ E-mail:simmhulh@mail.shcnc.ac.cn)
Abstract (Browse 2511)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
 

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