September 2012, Volume 54 Issue 9, Pages 610ĘC673.


Cover Caption: Heat Shock-Induced DREB2C Expression
About the cover: DREB is a family of transcription factors that function in coping with abiotic stresses. Chen et al. (pp. 640ĘC651) showed using a GUS reporter line that the expression of DREB2 is strongly induced by heat stress. The heat shock-induced expression is predominantly in young tissues and vascular bundles.

 

          Invited Expert Reviews
The Maternal-to-Zygotic Transition in Higher Plants
Author: Haiping Xin, Jing Zhao and Meng-Xiang Sun
Journal of Integrative Plant Biology 2012 54(9): 610-615
Published Online: June 26, 2012
DOI: 10.1111/j.1744-7909.2012.01138.x
      
    

During early embryogenesis in mammals and higher plants, the maternal-to-zygotic transition (MZT) marks the turnover of developmental control from maternal products to de novo zygotic genome transcripts. Intensive studies in animals indicate that early embryonic development is largely maternally controlled. In recent years, the MZT has drawn the attention of botanists, as it is important for understanding the mechanism of embryogenesis and hybrid vigor. In this study, we present a brief overview of some aspects of the MZT in flowering plants. Based on what we have learned from Nicotiana tabacum, we hypothesize that the MZT occurs before zygotic cell division and that the development of the fertilized egg cell in flowering plants can be divided into two phases: the zygote stage, which is mainly controlled maternally, and the one-celled proembryo stage, in which zygotic genome activation (ZGA) occurs and is required for zygote division.

Xin HP, Zhao J, Sun MX (2012) The maternal-to-zygotic transition in higher plants. J. Integr. Plant Biol. 54(9), 610–615.

Abstract (Browse 1662)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
          Cell and Developmental Biology
Genome-wide Transcription Factor Gene Prediction and their Expressional Tissue-Specificities in Maize  
Author: Yi Jiang, Biao Zeng, Hainan Zhao, Mei Zhang, Shaojun Xie and Jinsheng Lai
Journal of Integrative Plant Biology 2012 54(9): 616-630
Published Online: August 3, 2012
DOI: 10.1111/j.1744-7909.2012.01149.x
      
    

Transcription factors (TFs) are important regulators of gene expression. To better understand TF-encoding genes in maize (Zea mays L.), a genome-wide TF prediction was performed using the updated B73 reference genome. A total of 2 298 TF genes were identified, which can be classified into 56 families. The largest family, known as the MYB superfamily, comprises 322 MYB and MYB-related TF genes. The expression patterns of 2 014 (87.64%) TF genes were examined using RNA-seq data, which resulted in the identification of a subset of TFs that are specifically expressed in particular tissues (including root, shoot, leaf, ear, tassel and kernel). Similarly, 98 kernel-specific TF genes were further analyzed, and it was observed that 29 of the kernel-specific genes were preferentially expressed in the early kernel developmental stage, while 69 of the genes were expressed in the late kernel developmental stage. Identification of these TFs, particularly the tissue-specific ones, provides important information for the understanding of development and transcriptional regulation of maize.

Jiang Y, Zeng B, Zhao H, Zhang M, Xie S, Lai J (2012) Genome-wide transcription factor gene prediction and their expressional tissue-specificities in maize. J. Integr. Plant Biol. 54(9), 616–630.

Abstract (Browse 1857)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
          Plant-environmental Interactions
Overexpression of OsPAP10a, A Root-Associated Acid Phosphatase, Increased Extracellular Organic Phosphorus Utilization in Rice
Author: Jingluan Tian, Chuang Wang, Qian Zhang, Xiaowei He, James Whelan and Huixia Shou
Journal of Integrative Plant Biology 2012 54(9): 631-639
Published Online: July 16, 2012
DOI: 10.1111/j.1744-7909.2012.01143.x
      
    

Phosphorus (P) deficiency is a major limitation for plant growth and development. Among the wide set of responses to cope with low soil P, plants increase their level of intracellular and secreted acid phosphatases (APases), which helps to catalyze inorganic phosphate (Pi) hydrolysis from organo-phosphates. In this study we characterized the rice (Oryza sativa) purple acid phosphatase 10a (OsPAP10a). OsPAP10a belongs to group Ia of purple acid phosphatases (PAPs), and clusters with the principal secreted PAPs in a variety of plant species including Arabidopsis. The transcript abundance of OsPAP10a is specifically induced by Pi deficiency and is controlled by OsPHR2, the central transcription factor controlling Pi homeostasis. In gel activity assays of root and shoot protein extracts, it was revealed that OsPAP10a is a major acid phosphatase isoform induced by Pi starvation. Constitutive overexpression of OsPAP10a results in a significant increase of phosphatase activity in both shoot and root protein extracts. In vivo root 5-bromo-4-chloro-3-indolyl-phosphate (BCIP) assays and activity measurements on external media showed that OsPAP10a is a root-associated APase. Furthermore, overexpression of OsPAP10a significantly improved ATP hydrolysis and utilization compared with wild type plants. These results indicate that OsPAP10a can potentially be used for crop breeding to improve the efficiency of P use.

Tian J, Wang C, Zhang Q, He X, Whelan J, Shou H (2012) Overexpression of OsPAP10a, a root-associated acid phosphatase, increased extracellular organic phosphorus utilization in rice. J. Integr. Plant Biol. 54(9), 631–639.

Abstract (Browse 1841)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
A Proximal Promoter Region of Arabidopsis DREB2C Confers Tissue-specific Expression under Heat Stress
Author: Huan Chen, Jihyun Je, Chieun Song, Jung Eun Hwang and Chae Oh Lim
Journal of Integrative Plant Biology 2012 54(9): 640-651
Published Online: June 20, 2012
DOI: 10.1111/j.1744-7909.2012.01137.x
      
    

The dehydration-responsive element-binding factor 2C (DREB2C) is a member of the CBF/DREB subfamily of proteins, which contains a single APETALA2/Ethylene responsive element-binding factor (AP2/ERF) domain. To identify the expression pattern of the DREB2C gene, which contains multiple transcription cis-regulatory elements in its promoter, an approximately 1.4 kb upstream DREB2C sequence was fused to the β-glucuronidase reporter gene (GUS) and the recombinant p1244 construct was transformed into Arabidopsis thaliana (L.) Heynh. The promoter of the gene directed prominent GUS activity in the vasculature in diverse young dividing tissues. Upon applying heat stress (HS), GUS staining was also enhanced in the vasculature of the growing tissues. Analysis of a series of 5′-deletions of the DREB2C promoter revealed that a proximal upstream sequence sufficient for the tissue-specific spatial and temporal induction of GUS expression by HS is localized in the promoter region between −204 and −34 bps relative to the transcriptional start site. Furthermore, electrophoretic mobility shift assay (EMSA) demonstrated that nuclear protein binding activities specific to a −120 to −32 bp promoter fragment increased after HS. These results indicate that the TATA-proximal region and some latent trans-acting factors may cooperate in HS-induced activation of the Arabidopsis DREB2C promoter.

Chen H, Je J, Song C, Hwang JE, Lim CO (2012) A proximal promoter region of Arabidopsis DREB2C confers tissue-specific expression under heat stress. J. Integr. Plant Biol. 54(9), 640–651.

Abstract (Browse 1664)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
Overexpression of Rice Sphingosine-1-Phoshpate Lyase Gene OsSPL1 in Transgenic Tobacco Reduces Salt and Oxidative Stress Tolerance
Author: Huijuan Zhang, Jing Zhai, Jibo Mo, Dayong Li and Fengming Song
Journal of Integrative Plant Biology 2012 54(9): 652-662
Published Online: September 13, 2012
DOI: 10.1111/j.1744-7909.2012.01150.x
      
    

Sphingolipids, including sphingosine-1-phosphate (S1P), have been shown to function as signaling mediators to regulate diverse aspects of plant growth, development, and stress response. In this study, we performed functional analysis of a rice (Oryza sativa) S1P lyase gene OsSPL1 in transgenic tobacco plants and explored its possible involvement in abiotic stress response. Overexpression of OsSPL1 in transgenic tobacco resulted in enhanced sensitivity to exogenous abscisic acid (ABA), and decreased tolerance to salt and oxidative stress, when compared with the wild type. Furthermore, the expression levels of some selected stress-related genes in OsSPL1-overexpressing plants were reduced after application of salt or oxidative stress, indicating that the altered responsiveness of stress-related genes may be responsible for the reduced tolerance in OsSPL1-overexpressing tobacco plants under salt and oxidative stress. Our results suggest that rice OsSPL1 plays an important role in abiotic stress responses.

Zhang H, Zhai J, Mo J, Li D, Song F (2012) Overexpression of rice sphingosine-1-phoshpate lyase gene OsSPL1 in transgenic tobacco reduces salt and oxidative stress tolerance. J. Integr. Plant Biol. 54(9), 652–662.

Abstract (Browse 1469)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
          Molecular Physiology
Proanthocyanidins Inhibit Seed Germination by Maintaining High Level of Abscisic Acid in Arabidopsis thaliana  
Author: Liguo Jia, Qiuyu Wu, Nenghui Ye, Rui Liu, Lu Shi, Weifeng Xu, Hui Zhi, A. N. M. Rubaiyath Bin Rahman, Yiji Xia and Jianhua Zhang
Journal of Integrative Plant Biology 2012 54(9): 663-673
Published Online: July 5, 2012
DOI: 10.1111/j.1744-7909.2012.01142.x
      
    

Proanthocyanidins (PAs) are the main products of the flavonoid biosynthetic pathway in seeds, but their biological function during seed germination is still unclear. We observed that seed germination is delayed with the increase of exogenous PA concentration in Arabidopsis. A similar inhibitory effect occurred in peeled Brassica napus seeds, which was observed by measuring radicle elongation. Using abscisic acid (ABA), a biosynthetic and metabolic inhibitor, and gene expression analysis by real-time polymerase chain reaction, we found that the inhibitory effect of PAs on seed germination is due to their promotion of ABA via de novo biogenesis, rather than by any inhibition of its degradation. Consistent with the relationship between PA content and ABA accumulation in seeds, PA-deficient mutants maintain a lower level of ABA compared with wild-types during germination. Our data suggest that PA distribution in the seed coat can act as a doorkeeper to seed germination. PA regulation of seed germination is mediated by the ABA signaling pathway.

Jia L, Wu Q, Ye N, Liu R, Shi L, Xu W, Zhi H, Rahman ANMRB, Xia Y, Zhang J (2012) Proanthocyanidins inhibit seed germination by maintaining a high level of abscisic acid in Arabidopsis thaliana. J. Integr. Plant Biol. 54(9), 663–673.

Abstract (Browse 2273)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
 

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