January 1900, Volume 待分类 Issue 待分类


          Letters to the Editor
Differential requirement of BAK1 C-terminal tail in development and immunity
Author: Di Wu, Yanan Liu, Fan Xu and Yuelin Zhang
Journal of Integrative Plant Biology 1900 待分类(待分类): 1-6
Published Online: December 11, 2017
DOI: 10.1111/jipb.12623

BRI1-ASSOCIATED RECEPTOR KINASE 1 (BAK1) plays critical roles in plant developmental and immune signaling pathways. BAK1 and a large number of leucine-rich repeat receptor-like kinases (LRR-RLKs) harbor a mysterious carboxyl-terminal tail (CT) beyond their kinase domain. In this study we analyzed the biological significance of this CT region using a unique bak1 mutant allele which causes deletion of the CT region. We showed that BAK1 CT promotes its kinase activity and is required for pathogen-associated molecular pattern (PAMP)-triggered immunity, but it is dispensable for brassinosteroid responses and BAK1/BKK1-inhibited cell death signaling. Therefore the BAK1 C-terminal tail is differentially required for its functions in development and immunity.

Abstract (Browse 85)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
Multigene editing via CRISPR/Cas9 guided by a single-sgRNA seed in Arabidopsis  
Author: Zhiming Yu, Qiyuan Chen, Weiwei Chen, Xian Zhang, Fengling Mei, Pengcheng Zhang, Mei Zhao, Xiaohong Wang, Nongnong Shi, Stephen Jackson and Yiguo Hong
Journal of Integrative Plant Biology 1900 待分类(待分类): 1-7
Published Online: December 11, 2017
DOI: 10.1111/jipb.12622

We report that a solo single-guide RNA (sgRNA) seed is capable of guiding Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR −associated 9 (CRISRP/Cas9) to simultaneously edit multiple genes AtRPL10A, AtRPL10B and AtRPL10C in Arabidopsis. Our results also demonstrate that it is possible to use CRISPR/Cas9 technology to create AtRPL10 triple mutants which otherwise cannot be generated by conventional genetic crossing. Compared to other conventional multiplex CRISPR/Cas systems, a single sgRNA seed has the advantage of reducing off-target gene-editing. Such a gene editing system might be also applicable to modify other homologous genes, or even less-homologous sequences for multiple gene-editing in plants and other organisms.

Abstract (Browse 84)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
Generation of new glutinous rice by CRISPR/Cas9-targeted mutagenesis of the Waxy gene in elite rice varieties
Author: Jinshan Zhang, Hui Zhang, José Ramón Botella and Jian-Kang Zhu
Journal of Integrative Plant Biology 1900 待分类(待分类): 1-7
Published Online: December 6, 2017
DOI: 10.1111/jipb.12620

In rice, amylose content (AC) is controlled by a single dominant Waxy gene. We used Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated 9 (Cas9) to introduce a loss-of-function mutation into the Waxy gene in two widely cultivated elite japonica varieties. Our results show that mutations in the Waxy gene reduce AC and convert the rice into glutinous ones without affecting other desirable agronomic traits, offering an effective and easy strategy to improve glutinosity in elite varieties. Importantly, we successfully removed the transgenes from the progeny. Our study provides an example of generating improved crops with potential for commercialization, by editing a gene of interest directly in elite crop varieties.

Abstract (Browse 90)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
RPL18aB helps maintain suspensor identity during early embryogenesis
Author: Fei Xie, Hailong Yan, Yang Sun, Yameng Wang, Hong Chen, Wanying Mao, Liyao Zhang, Mengxiang Sun and Xiongbo Peng
Journal of Integrative Plant Biology 1900 待分类(待分类): 1-5
Published Online: November 29, 2017
DOI: 10.1111/jipb.12616

During embryogenesis, plants are thought to use a mechanism that allows the suspensor to maintain its identity. Here, we reported that RPL18aB is involved in this mechanism in Arabidopsis thaliana. The suspensor cells proliferated in rpl18aB and formed a multicellular structure rather than undergo programmed cell death, as in wild type. Suspensors of rpl18aB expressed the embryo proper marker, DRN::GFP, but not the suspensor marker, WOX8::GFP. In addition, auxin accumulated throughout the suspensors of rpl18aB proembryos. Suspensor-specific expression of RPL18aB could rescue the cell proliferation defects in rpl18aB suspensors. These findings supported a role for RPL18aB in maintaining suspensor identity.

Abstract (Browse 109)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
Jasmonate signaling makes flowers attractive to pollinators and repellant to florivores in nature
Author: Ran Li, Meredith C. Schuman, Yang Wang, Lucas Cortés Llorca, Julia Bing, Anne Bennion, Rayko Halitschke and Ian T. Baldwin
Journal of Integrative Plant Biology 1900 待分类(待分类): 1-5
Published Online: October 23, 2017
DOI: 10.1111/jipb.12607

Flowers are required for the Darwinian fitness of flowering plants, but flowers’ advertisements for pollination services can attract florivores. Previous glasshouse work with Nicotiana attenuata revealed the role of jasmonate (JA) signaling in flower development, advertisement and defense. However, whether JA signaling mediates flowers’ filtering of floral visitors in nature remained unknown. This field study revealed that silencing JA signaling resulted in flowers that produce less nectar and benzyl acetone, two pollinator-attractive traits. Meanwhile, flowers of defenseless plants were highly attacked by a suite of native herbivores, and damage to buds in native plants correlated negatively with their JA-Ile levels.


Abstract (Browse 120)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
          New Resources
Establishment and evaluation of a peanut association panel and analysis of key nutritional traits
Author: Xiurong Zhang, Suqing Zhu, Kun Zhang, Yongshan Wan, Fengzhen Liu, Qingfang Sun and Yingjie Li
Journal of Integrative Plant Biology 1900 待分类(待分类): 1-21
Published Online: October 4, 2017
DOI: 10.1111/jipb.12601

Breeding programs aim to improve the yield and quality of peanut (Arachis hypogaea L.); using association mapping to identify genetic markers linked to these quantitative traits could facilitate selection efficiency. A peanut association panel was established consisting of 268 lines with extensive phenotypic and genetic variation, meeting the requirements for association analysis. These lines were grown over 3 years and the key agronomic traits, including protein and oil content were examined. Population structure (Q) analysis showed two subpopulations and clustering analysis was consistent with Q-based membership assignment and closely related to botanical type. Relative Kinship (K) indicated that most of the panel members have no or weak familial relatedness, with 52.78% of lines showing K = 0. Linkage disequilibrium (LD) analysis showed a high level of LD occurs in the panel. Model comparisons indicated false positives can be effectively controlled by taking Q and K into consideration and more false positives were generated by K than Q. A preliminary association analysis using a Q + K model found markers significantly associated with oil, protein, oleic acid, and linoleic acid, and identified a set of alleles with positive and negative effects. These results show that this panel is suitable for association analysis, providing a resource for marker-assisted selection for peanut improvement.

Abstract (Browse 110)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
          Plant-abiotic Interactions
Nicotiana attenuata's capacity to interact with arbuscular mycorrhiza alters its competitive ability and elicits major changes in the leaf transcriptome
Author: Ming Wang, Julia Wilde, Ian T. Baldwin and Karin Groten
Journal of Integrative Plant Biology 1900 待分类(待分类): 1-20
Published Online: October 31, 2017
DOI: 10.1111/jipb.12609

To study the local and systemic effects of arbuscular mycorrhizal fungal (AMF) colonization, Nicotiana attenuata plants impaired in their interactions with AMF due to silencing of a calcium- and calmodulin dependent protein kinase (inverted repreat (ir)CCaMK) were grown competitively in pairs with empty vector (EV) plants, with and without two different types of inoculum. When inoculated, EV plants strongly outperformed irCCaMK plants. Foliar transcript profiling revealed that AMF colonization significantly changed gene expression of P-starvation and -transporter genes in irCCaMK plants. The Pht1 family phosphate transporter NaPT5 was not only specifically induced in roots after AMF colonization, but also in leaves of AMF-colonized irCCaMK plants, and in plants grown under low Pi conditions in the absence of AMF. The P-starvation signature of inoculated irCCaMK plants corresponded with increases in selected amino acids and phenolic compounds in leaves. We also found a strong AMF-induced increase in amino acids and phenolic metabolites in roots. Plants impaired in their interactions with AMF clearly have a fitness disadvantage when competing for limited soil nutrients with a fully functional isogenic line. The additional role of the AMF-induced Pht1 family transporter NaPT5 in leaves under P-starvation conditions will require further experiments to fully resolve.

Abstract (Browse 69)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
Characterization of the soybean GmALMT family genes and the function of GmALMT5 in response to phosphate starvation
Author: Wenting Peng, Weiwei Wu, Junchu Peng, Jiaojiao Li, Yan Lin, Yanan Wang, Jiang Tian, Lili Sun, Cuiyue Liang and Hong Liao
Journal of Integrative Plant Biology 1900 待分类(待分类): 1-16
Published Online: October 17, 2017
DOI: 10.1111/jipb.12604

A potential mechanism to enhance utilization of sparingly soluble forms of phosphorus (P) is the root secretion of malate, which is mainly mediated by the ALMT gene family in plants. In this study, a total of 34 GmALMT genes were identified in the soybean genome. Expression patterns diverged considerably among GmALMTs in response to phosphate (Pi) starvation in leaves, roots and flowers, with expression altered by P availability in 26 of the 34 GmALMTs. One root-specific GmALMT whose expression was significantly enhanced by Pi-starvation, GmALMT5, was studied in more detail to determine its possible role in soybean P nutrition. Analysis of GmALMT5 tissue expression patterns, subcellular localization, and malate exudation from transgenic soybean hairy roots overexpressing GmALMT5, demonstrated that GmALMT5 is a plasma membrane protein that mediates malate efflux from roots. Furthermore, both growth and P content of transgenic Arabidopsis overexpressing GmALMT5 were significantly increased when sparingly soluble Ca-P was used as the external P source. Taken together, these results indicate that members of the soybean GmALMT gene family exhibit diverse responses to Pi starvation. One member of this family, GmALMT5, might contribute to soybean P efficiency by enhancing utilization of sparingly soluble P sources under P limited conditions.

Abstract (Browse 83)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
          Molecular Physiology
Quantitative trait loci associated with soybean seed weight and composition under different phosphorus levels
Author: Gokhan Hacisalihoglu, Amy L. Burton, Jeffery L. Gustin, Selim Eker, Safiye Asikli, Elif Hakli Heybet, Levent Ozturk, Ismail Cakmak, Atilla Yazici, Kent O. Burkey, James Orf and A. Mark Settles
Journal of Integrative Plant Biology 1900 待分类(待分类): 1-11
Published Online: November 13, 2017
DOI: 10.1111/jipb.12612

Seed size and composition are important traits in food crops and can be affected by nutrient availability in the soil. Phosphorus (P) is a non-renewable, essential macronutrient, and P deficiency limits soybean (Glycine max) yield and quality. To investigate the associations of seed traits in low- and high-P environments, soybean recombinant inbred lines (RILs) from a cross of cultivars Fiskeby III and Mandarin (Ottawa) were grown under contrasting P availability environments. Traits including individual seed weight, seed number, and intact mature pod weight were significantly affected by soil P levels and showed transgressive segregation among the RILs. Surprisingly, P treatments did not affect seed composition or weight, suggesting that soybean maintains sufficient P in seeds even in low-P soil. Quantitative trait loci (QTLs) were detected for seed weight, intact pods, seed volume, and seed protein, with five significant QTLs identified in low-P environments and one significant QTL found in the optimal-P environment. Broad-sense heritability estimates were 0.78 (individual seed weight), 0.90 (seed protein), 0.34 (seed oil), and 0.98 (seed number). The QTLs identified under low P point to genetic regions that may be useful to improve soybean performance under limiting P conditions.

Abstract (Browse 79)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
          Functional Omics and Systems Biology
Metabolic responses of Eucalyptus species to different temperature regimes  
Author: Joao Benhur Mokochinski, Paulo Mazzafera, Alexandra Christine Helena Frankland Sawaya, Roland Mumm, Ric Cornelis Hendricus de Vos and Robert David Hall
Journal of Integrative Plant Biology 1900 待分类(待分类): 1-15
Published Online: December 16, 2017
DOI: 10.1111/jipb.12626

Species and hybrids of Eucalyptus are the world's most widely planted hardwood trees. They are cultivated across a wide range of latitudes and therefore environmental conditions. In this context, comprehensive metabolomics approaches have been used to assess how different temperature regimes may affect the metabolism of three species of Eucalyptus, E. dunnii, E. grandis and E. pellita. Young plants were grown for 53 d in the greenhouse and then transferred to growth chambers at 10°C, 20°C or 30°C for another 7 d. In all three species the leaf chlorophyll content was positively correlated to temperature, and in E. pellita the highest temperature also resulted in a significant increase in stem biomass. Comprehensive metabolomics was performed using untargeted gas chromatography mass spectrometry (GC-MS) and liquid chromatography (LC)-MS. This approach enabled the comparison of the relative abundance of 88 polar primary metabolites from GC-MS and 625 semi-polar secondary metabolites from LC-MS. Using principal components analysis, a major effect of temperature was observed in each species which was larger than that resulting from the genetic background. Compounds mostly affected by temperature treatment were subsequently selected using partial least squares discriminant analysis and were further identified. These putative annotations indicated that soluble sugars and several polyphenols, including tannins, triterpenes and alkaloids were mostly influenced.

Abstract (Browse 74)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
          Cell and Developmental Biology
Natural variation of hormone levels in Arabidopsis roots and correlations with complex root architecture  
Author: Sangseok Lee, Lidiya I. Sergeeva and Dick Vreugdenhil
Journal of Integrative Plant Biology 1900 待分类(待分类): 1-18
Published Online: December 2, 2017
DOI: 10.1111/jipb.12617

Studies on natural variation are an important tool to unravel the genetic basis of quantitative traits in plants. Despite the significant roles of phytohormones in plant development, including root architecture, hardly any studies have been done to investigate natural variation in endogenous hormone levels in plants. Therefore, in the present study a range of hormones were quantified in root extracts of thirteen Arabidopsis thaliana accessions using a ultra performance liquid chromatography triple quadrupole mass spectrometer. Root system architecture of the set of accessions was quantified, using a new parameter (mature root unit) for complex root systems, and correlated with the phytohormone data. Significant variations in phytohormone levels among the accessions were detected, but were remarkably small, namely less than three-fold difference between extremes. For cytokinins, relatively larger variations were found for ribosides and glucosides, as compared to the free bases. For root phenotyping, length-related traits—lateral root length and total root length—showed larger variations than lateral root number-related ones. For root architecture, antagonistic interactions between hormones, for example, indole-3-acetic acid to trans-zeatin were detected in correlation analysis. These findings provide conclusive evidence for the presence of natural variation in phytohormone levels in Arabidopsis roots, suggesting that quantitative genetic analyses are feasible.

Abstract (Browse 79)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       


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