January 1900, Volume 待分类 Issue 待分类


          Letters to the Editor
Efficient allelic replacement in rice by gene editing: A case study of the NRT1.1B gene  
Author: Jingying Li, Xin Zhang, Yongwei Sun, Jiahui Zhang, Wenming Du, Xiuping Guo, Shaoya Li, Yunde Zhao, Lanqin Xia
Journal of Integrative Plant Biology 1900 待分类(待分类): 1-5
Published Online: March 23, 2018
DOI: 10.1111/jipb.12650

Precise replacement of an existing allele in commercial cultivars with an elite allele is a major goal in crop breeding. A single nucleotide polymorphism in the NRT1.1B gene between japonica and indica rice is responsible for the improved nitrogen use efficiency in indica rice. Herein, we precisely replaced the japonica NRT1.1B allele with the indica allele, in just one generation, using CRISPR/Cas9 gene‐editing technology. No additional selective pressure was needed to enrich the precise replacement events. This work demonstrates the feasibility of replacing any genes with elite alleles within one generation, greatly expanding our ability to improve agriculturally important traits.

Abstract (Browse 186)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
SDG721 and SDG705 are required for rice growth
Author: Pengfei Jiang, Shiliang Wang, Aziz Ul Ikram, Zuntao Xu, Haiyang Jiang, Beijiu Cheng and Yong Ding
Journal of Integrative Plant Biology 1900 待分类(待分类): 1-7
Published Online: February 23, 2018
DOI: 10.1111/jipb.12644

H3K4me3 plays important roles in development, transcription, and environmental responses. Here, we report that SDG721 (SET‐domain group protein 721) and SDG705 are involved in regulating rice development. SDG721 and SDG705 encode TRITHORAX‐like proteins, which appear to modulate H3K4 methylation levels. Loss of SDG721 and SDG705 function resulted in GA‐deficient phenotypes, including semi‐dwarfism, reduced cell length, and reduced panicle branching. The transcripts levels and H3K4me3 levels of GA biosynthesis genes and GA signaling pathway genes were downregulated in the sdg721 sdg705 plants. Together, these results suggest that SDG721 and SDG705 regulate H3K4 methylation, which is crucial for plant development in rice.

Abstract (Browse 220)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
          New Technology
Calcium imaging in Arabidopsis pollen cells using G‐CaMP5
Author: Min Diao, Xiaolu Qu and Shanjin Huang
Journal of Integrative Plant Biology 1900 待分类(待分类): 1-10
Published Online: February 9, 2018
DOI: 10.1111/jipb.12642

Calcium (Ca2+) signaling has been implicated in pollen germination and pollen tube growth. To date, however, we still know very little about how exactly Ca2+ signaling links to various physiological subcellular processes during pollen germination and pollen tube growth. Given that Ca2+ signaling is tightly related to the cytosolic concentration and dynamics of Ca2+, it is vital to trace the dynamic changes in Ca2+ levels in order to decode Ca2+ signaling. Here, we demonstrate that G‐CaMP5 serves well as an indicator for monitoring cytosolic Ca2+ dynamics in pollen cells. Using this probe, we show that cytosolic Ca2+ changes dramatically during pollen germination, and, as reported previously, Ca2+ forms a tip‐focused gradient in the pollen tube and undergoes oscillation in the tip region during pollen tube growth. In particular, using G‐CaMP5 allowed us to capture the dynamic changes in the cytosolic Ca2+ concentration ([Ca2+]cyt) in pollen tubes in response to various exogenous treatments. Our data suggest that G‐CaMP5 is a suitable probe for monitoring the dynamics of [Ca2+]cyt in pollen cells.

Abstract (Browse 197)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
          Molecular Physiology
Two soybean bHLH factors regulate response to iron deficiency
Author: Lin Li, Wenwen Gao, Qi Peng, Bin Zhou, Qihui Kong, Yinghui Ying, Huixia Shou
Journal of Integrative Plant Biology 1900 待分类(待分类): 1-15
Published Online: March 23, 2018
DOI: 10.1111/jipb.12651

Iron is an indispensable micronutrient for plant growth and development. Limited bioavailability of Fe in the soil leads to iron deficiency chlorosis in plants and yield loss. In this study, two soybean basic helix‐loop‐helix transcription factors, GmbHLH57 and GmbHLH300, were identified in response to Fe‐deficiency. Both transcription factors are expressed in roots and nodules, and are induced by Fe deficiency; these patterns were confirmed in transgenic hairy roots expressing constructs of the endogenous promoters fused to a GUS reporter gene. Bimolecular fluorescence complementation, yeast two‐hybrid and coimmunoprecipitation (co‐IP) assays indicated a physical interaction between GmbHLH57 and GmbHLH300. Studies on transgenic soybeans overexpressing GmbHLH57 and GmbHLH300 revealed that overexpression of each transcription factor, alone, results in no change of the responses to Fe deficiency, whereas overexpression of both transcription factors upregulated the downstream Fe uptake genes and increased the Fe content in these transgenic plants. Compared to wild type, these double overexpression transgenic plants were more tolerant to Fe deficiency. Taken together, our findings establish that GmbHLH57 and GmbHLH300 are important transcription factors involved in Fe homeostasis in soybean.

Abstract (Browse 62)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
The Arabidopsis catalase triple mutant reveals important roles of catalases and peroxisome‐derived signaling in plant development
Author: Tong Su, Pingping Wang, Huijuan Li, Yiwu Zhao, Yao Lu, Peng Dai, Tianqi Ren, Xiaofeng Wang, Xuezhi Li, Qun Shao, Dazhong Zhao, Yanxiu Zhao and Changle Ma
Journal of Integrative Plant Biology 1900 待分类(待分类): 1-17
Published Online: March 23, 2018
DOI: 10.1111/jipb.12649

Hydrogen peroxide (H2O2) is generated in many metabolic processes. As a signaling molecule, H2O2 plays important roles in plant growth and development, as well as environmental stress response. In Arabidopsis, there are three catalase genes, CAT1, CAT2, and CAT3. The encoded catalases are predominately peroxisomal proteins and are critical for scavenging H2O2. Since CAT1 and CAT3 are linked on chromosome 1, it has been almost impossible to generate cat1/3 and cat1/2/3 mutants by traditional genetic tools. In this study, we constructed cat1/3 double mutants and cat1/2/3 triple mutants by CRISPR/Cas9 to investigate the role of catalases. The cat1/2/3 triple mutants displayed severe redox disturbance and growth defects under physiological conditions compared with wild‐type and the cat2/3 double mutants. Transcriptome analysis showed a more profound transcriptional response in the cat1/2/3 triple mutants compared to the cat2/3 mutants. These differentially expressed genes are involved in plant growth regulation as well as abiotic and biotic stress responses. In addition, expression of OXI1 (OXIDATIVE SIGNAL INDUCIBLE 1) and several MAPK cascade genes were changed dramatically in the catalase triple mutant, suggesting that H2O2 produced in peroxisomes could serve as a peroxisomal retrograde signal.

Abstract (Browse 105)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
Phot2‐regulated relocation of NPH3 mediates phototropic response to high‐intensity blue light in Arabidopsis thaliana
Author: Xiang Zhao, Qingping Zhao, Chunye Xu, Jin Wang, Jindong Zhu, Baoshuan Shang and Xiao Zhang
Journal of Integrative Plant Biology 1900 待分类(待分类): 1-16
Published Online: February 2, 2018
DOI: 10.1111/jipb.12639

Two redundant blue‐light receptors, known as phototropins (phot1 and phot2), influence a variety of physiological responses, including phototropism, chloroplast positioning, and stomatal opening in Arabidopsis thaliana. Whereas phot1 functions in both low‐ and high‐intensity blue light (HBL), phot2 functions primarily in HBL. Here, we aimed to elucidate phot2‐specific functions by screening for HBL‐insensitive mutants among mutagenized Arabidopsis phot1 mutants. One of the resulting phot2 signaling associated (p2sa) double mutants, phot1 p2sa2, exhibited phototropic defects that could be restored by constitutively expressing NON‐PHOTOTROPIC HYPOCOTYL 3 (NPH3), indicating that P2SA2 was allelic to NPH3. It was observed that NPH3‐GFP signal mainly localized to and clustered on the plasma membrane in darkness. This NPH3 clustering on the plasma membrane was not affected by mutations in genes encoding proteins that interact with NPH3, including PHOT1, PHOT2 and ROOT PHOTOTROPISM 2 (RPT2). However, the HBL irradiation‐mediated release of NPH3 proteins into the cytoplasm was inhibited in phot1 mutants and enhanced in phot2 and rpt2‐2 mutants. Furthermore, HBL‐induced hypocotyl phototropism was enhanced in phot1 mutants and inhibited in the phot2 and rpt2‐2 mutants. Our findings indicate that phot1 regulates the dissociation of NPH3 from the plasma membrane, whereas phot2 mediates the stabilization and relocation of NPH3 to the plasma membrane to acclimate to HBL.

Abstract (Browse 204)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
          Cell and Developmental Biology
Plant G proteins interact with endoplasmic reticulum luminal protein receptors to regulate endoplasmic reticulum retrieval  
Author: Shanshan Wang, Ke Xie, Guoyong Xu, Huarui Zhou, Qiang Guo, Jingyi Wu, Zengwei Liao, Na Liu, Yan Wang and Yule Liu
Journal of Integrative Plant Biology 1900 待分类(待分类): 1-21
Published Online: March 20, 2018
DOI: 10.1111/jipb.12648

Maintaining endoplasmic reticulum (ER) homeostasis is essential for the production of biomolecules. ER retrieval, i.e., the retrograde transport of compounds from the Golgi to the ER, is one of the pathways that ensures ER homeostasis. However, the mechanisms underlying the regulation of ER retrieval in plants remain largely unknown. Plant ERD2‐like proteins (ERD2s) were recently suggested to function as ER luminal protein receptors that mediate ER retrieval. Here, we demonstrate that heterotrimeric G protein signaling is involved in ERD2‐mediated ER retrieval. We show that ERD2s interact with the heterotrimeric G protein Gα and Gγ subunits at the Golgi. Silencing of , , or increased the retention of ER luminal proteins. Furthermore, overexpression of Gα, Gβ, or Gγ caused ER luminal proteins to escape from the ER, as did the co‐silencing of ERD2a and ERD2b. These results suggest that G proteins interact with ER luminal protein receptors to regulate ER retrieval.

Abstract (Browse 70)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
          Functional Omics and Systems Biology
Transcriptional and temporal response of Populus stems to gravi‐stimulation
Author: Matthew Zinkgraf, Suzanne Gerttula, Shutang Zhao, Vladimir Filkov and AndrewLi Groover
Journal of Integrative Plant Biology 1900 待分类(待分类): 1-14
Published Online: February 26, 2018
DOI: 10.1111/jipb.12645

Plants modify development in response to external stimuli, to produce new growth that is appropriate for environmental conditions. For example, gravi‐stimulation of leaning branches in angiosperm trees results in modifications of wood development, to produce tension wood that pulls leaning stems upright. Here, we use gravi‐stimulation and tension wood response to dissect the temporal changes in gene expression underlying wood formation in Populus stems. Using time‐series analysis of seven time points over a 14‐d experiment, we identified 8,919 genes that were differentially expressed between tension wood (upper) and opposite wood (lower) sides of leaning stems. Clustering of differentially expressed genes showed four major transcriptional responses, including gene clusters whose transcript levels were associated with two types of tissue‐specific impulse responses that peaked at about 24–48 h, and gene clusters with sustained changes in transcript levels that persisted until the end of the 14‐d experiment. Functional enrichment analysis of those clusters suggests they reflect temporal changes in pathways associated with hormone regulation, protein localization, cell wall biosynthesis and epigenetic processes. Time‐series analysis of gene expression is an underutilized approach for dissecting complex developmental responses in plants, and can reveal gene clusters and mechanisms influencing development.

Abstract (Browse 114)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
          Plant-abiotic Interactions
Hormone modulation of legume‐rhizobial symbiosis
Author: Huan Liu, Chi Zhang, Jun Yang, Nan Yu and Ertao Wang
Journal of Integrative Plant Biology 1900 待分类(待分类): 1-17
Published Online: March 26, 2018
DOI: 10.1111/jipb.12653

Leguminous plants can establish symbiotic associations with diazotropic rhizobia to form nitrogen‐fixating nodules, which are classified as determinate or indeterminate based on the persistence of nodule meristem. The formation of nitrogen‐fixing nodules requires coordinating rhizobial infection and root nodule organogenesis. The formation of an infection thread and the extent of nodule formation are largely under plant control, but vary with environmental conditions and the physiological state of the host plants. Many achievements in these two areas have been made in recent decades. Phytohormone signaling pathways have gradually emerged as important regulators of root nodule symbiosis. Cytokinin, strigolactones (SLs) and local accumulation of auxin can promote nodule development. Ethylene, jasmonic acid (JA), abscisic acid (ABA) and gibberellic acid (GA) all negatively regulate infection thread formation and nodule development. However, salicylic acid (SA) and brassinosteroids (BRs) have different effects on the formation of these two nodule types. Some peptide hormones are also involved in nodulation. This review summarizes recent findings on the roles of these plant hormones in legume‐rhizobial symbiosis, and we propose that DELLA proteins may function as a node to integrate plant hormones to regulate nodulation.

Abstract (Browse 90)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
          Plant Reproduction Biology
Arabidopsis thaliana NOP10 is required for gametophyte formation
Author: Lin-Xiao Li, Hong-Ze Liao, Li-Xi Jiang, Qing Tan, De Ye and Xue-Qin Zhang
Journal of Integrative Plant Biology 1900 待分类(待分类): 1-15
Published Online: March 26, 2018
DOI: 10.1111/jipb.12652

The female gametophyte is crucial for sexual reproduction of higher plants, yet little is known about the molecular mechanisms underlying its development. Here, we report that Arabidopsis thaliana NOP10 (AtNOP10) is required for female gametophyte formation. AtNOP10 was expressed predominantly in the seedling and reproductive tissues, including anthers, pollen grains, and ovules. Mutations in AtNOP10 interrupted mitosis of the functional megaspore during early development and prevented polar nuclear fusion in the embryo sacs. AtNOP10 shares a high level of amino acid sequence similarity with Saccharomyces cerevisiae (yeast) NOP10 (ScNOP10), an important component of the H/ACA small nucleolar ribonucleoprotein particles (H/ACA snoRNPs) implicated in 18S rRNA synthesis and rRNA pseudouridylation. Heterologous expression of ScNOP10 complemented the mutant phenotype of Atnop10. Thus, AtNOP10 influences functional megaspore mitosis and polar nuclear fusion during gametophyte formation in Arabidopsis.

Abstract (Browse 78)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
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