January 1900, Volume 待分类 Issue 待分类


          Special Issue: Genomics-assisted Germplasm Improvement in Rice
OsIDD2, a zinc finger and INDETERMINATE DOMAIN protein, regulates secondary cell wall formation
Author: Peng Huang, Hideki Yoshida, Kenji Yano, Shunsuke Kinoshita, Kyosuke Kawai, Eriko Koketsu, Masako Hattori, Sayaka Takehara, Ji Huang, Ko Hirano, Reynante Lacsamana Ordonio, Makoto Matsuoka and Miyako Ueguchi-Tanaka
Journal of Integrative Plant Biology 1900 待分类(待分类): 1-15
Published Online: June 2, 2017
DOI: 10.1111/jipb.12557

Previously, we found 123 transcription factors (TFs) as candidate regulators of secondary cell wall (SCW) formation in rice by using phylogenetic and co-expression network analyses. Among them, we examined in this work the role of OsIDD2, a zinc finger and indeterminate domain (IDD) family TF. Its overexpressors showed dwarfism, fragile leaves, and decreased lignin content, which are typical phenotypes of plants defective in SCW formation, whereas its knockout plants showed slightly increased lignin content. The RNA-seq and quantitative reverse transcription polymerase chain reaction analyses confirmed that some lignin biosynthetic genes were downregulated in the OsIDD2-overexpressing plants, and revealed the same case for other genes involved in cellulose synthesis and sucrose metabolism. The transient expression assay using rice protoplasts revealed that OsIDD2 negatively regulates the transcription of genes involved in lignin biosynthesis, cinnamyl alcohol dehydrogenase 2 and 3 (CAD2 and 3), and sucrose metabolism, sucrose synthase 5 (SUS5), whereas an AlphaScreen assay, which can detect the interaction between TFs and their target DNA sequences, directly confirmed the interaction between OsIDD2 and the target sequences located in the promoter regions of CAD2 and CAD3. Based on these observations, we conclude that OsIDD2 is negatively involved in SCW formation and other biological events by downregulating its target genes.

Abstract (Browse 131)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
Ectopic expression of fungal EcGDH improves nitrogen assimilation and grain yield in rice
Author: Dongying Tang, Yuchong Peng, Jianzhong Lin, Changqing Du, Yuanzhu Yang, Dan Wang, Cong Liu, Lu Yan, Xiaoying Zhao, Xia Li, Liangbi Chen and Xuanming Liu
Journal of Integrative Plant Biology 1900 待分类(待分类): 1-5
Published Online: January 6, 2017
DOI: 10.1111/jipb.12519

NADP(H)-dependent glutamate dehydrogenases (GDH) in lower organisms have stronger ammonium affinity than those in higher plants. Here we report that transgenic rice overexpressing the EcGDH from Eurotium cheralieri exhibited significantly enhanced aminating activities. Hydroponic and field tests showed that nitrogen assimilation efficiency and grain yields were markedly increased in these transgenic plants, especially at the low nitrogen conditions. These results suggest that EcGDH may have potential to be used to improve nitrogen assimilation and grain yield in rice.

Abstract (Browse 332)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
QTL editing confers opposing yield performance in different rice varieties
Author: Lan Shen, Chun Wang, Yaping Fu, Junjie Wang, Qing Liu, Xiaoming Zhang, Changjie Yan, Qian Qian and Kejian Wang
Journal of Integrative Plant Biology 1900 待分类(待分类): 1-4
Published Online: September 15, 2016
DOI: 10.1111/jipb.12501

Grain yield is one of the most important and complex trait for genetic improvement in crops; it is known to be controlled by a number of genes known as quantitative trait loci (QTLs). In the past decade, many yield-contributing QTLs have been identified in crops. However, it remains unclear whether those QTLs confer the same yield performance in different genetic backgrounds. Here, we performed CRISPR/Cas9-mediated QTL editing in five widely-cultivated rice varieties and revealed that the same QTL can have diverse, even opposing, effects on grain yield in different genetic backgrounds.

Abstract (Browse 404)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
          Letters to the Editor
Seed dispersal by hornets: An unusual insect-plant mutualism
Author: Gao Chen, Zheng-Wei Wang, Yan Qin and Wei-Bang Sun
Journal of Integrative Plant Biology 1900 待分类(待分类): 1-6
Published Online: July 11, 2017
DOI: 10.1111/jipb.12568

Vespicochory, seed dispersal by hornets, is a rare seed dispersal mechanism in angiosperms and, to date, there are few records of this phenomenon. Through field investigations and behavioral assays conducted in four populations of Stemona tuberosa from 2011–2016, we demonstrate that hornets are the primary seed dispersers of S. tuberosa and play an important role in “long-distance” seed dispersal in this species. Furthermore, some ant species act as secondary dispersers and may transport the seeds to safe sites. Hornets and ants provide complementary seed dispersal at different spatial scales. This unique example of insect-plant mutualism may be an underestimated but important strategy to ensure “long-distance” seed dispersal in other myrmecochorous plants.

Abstract (Browse 57)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
THESEUS1 positively modulates plant defense responses against Botrytis cinerea through GUANINE EXCHANGE FACTOR4 signaling
Author: Shaofeng Qu, Xi Zhang, Yutong Song, Jinxing Lin and Xiaoyi Shan
Journal of Integrative Plant Biology 1900 待分类(待分类): 1-8
Published Online: June 24, 2017
DOI: 10.1111/jipb.12565

The plant cell wall is an important interface for sensing pathogen attack and activating signaling pathways that promote plant immune responses. THESEUS1 (THE1) acts as a sensor of cell wall integrity that controls cell elongation during plant growth. However, no specific role for THE1 in plant defense responses has been reported. Here, we found that THE1 interacts with GUANINE EXCHANGE FACTOR4 (GEF4) and that both proteins play regulatory roles in plant resistance to the necrotrophic fungus Botrytis cinerea. Genetic analysis showed that THE1 and GEF4 function in the same genetic pathway to mediate plant defense responses. In addition, using transcriptome analysis, we identified various genes (such as defense-related, secondary metabolite-related, and transcription factor genes) that are likely downstream targets in the THE1-GEF4 signaling pathway. Our results suggest that THE1 functions as an upstream regulator of GEF4 signaling to positively regulate defense responses against B. cinerea in Arabidopsis.

Abstract (Browse 98)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
Arabidopsis TOR signaling is essential for sugar-regulated callus formation
Author: Kyounghee Lee and Pil Joon Seo
Journal of Integrative Plant Biology 1900 待分类(待分类): 1-6
Published Online: June 17, 2017
DOI: 10.1111/jipb.12560

Dedifferentiation is a remarkable process that produces pluripotent stem cells from differentiated somatic cells to ensure developmental plasticity. Plants have evolved the ability of cellular dedifferentiation, and signaling cascades related to auxin and cytokinin-dependent callus formation have been extensively investigated. However, the molecular mechanism underlying sugar-dependent callus formation remains unknown. Here, we show that sugar-dependent callus formation is mainly regulated by the TOR-E2Fa module in Arabidopsis. Sugar-activated TOR kinase phosphorylates and stabilizes E2Fa proteins to transcriptionally activate S-phase genes during callus formation. In parallel, E2Fa is transcriptionally regulated by the ARF-LBD transcription cascade. Multi-layered regulation of E2Fa by sugar and auxin is likely to shape balanced cellular dedifferentiation capability in Arabidopsis.

Abstract (Browse 117)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
Mutation in a novel gene SMALL AND CORDATE LEAF 1 affects leaf morphology in cucumber
Author: Dongli Gao, Chunzhi Zhang, Shu Zhang, Bowen Hu, Shenhao Wang, Zhonghua Zhang and Sanwen Huang
Journal of Integrative Plant Biology 1900 待分类(待分类): 1-7
Published Online: June 9, 2017
DOI: 10.1111/jipb.12558

Plant species exhibit substantial variation in leaf morphology. We isolated a recessive mutant gene termed small and cordate leaf 1 (scl1) that causes alteration in both leaf size and shape of cucumber. Compared to wild type leaves, the scl1 mutant had fewer numbers of epidermal pavement cells. A single nucleotide polymorphism was associated with this leaf phenotype, which occurred in a putative nucleoside bisphosphate phosphatase. RNA-seq analysis of the wild type and scl1 mutant leaves suggested that SCL1 regulation may not involve known hormonal pathways. Our work identified a candidate gene for SCL1 that may play a role in leaf development.

Abstract (Browse 120)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
          Invited Expert Review
Engineering crop nutrient efficiency for sustainable agriculture
Author: Liyu Chen and Hong Liao
Journal of Integrative Plant Biology 1900 待分类(待分类): 1-27
Published Online: June 10, 2017
DOI: 10.1111/jipb.12559

Increasing crop yields can provide food, animal feed, bioenergy feedstocks and biomaterials to meet increasing global demand; however, the methods used to increase yield can negatively affect sustainability. For example, application of excess fertilizer can generate and maintain high yields but also increases input costs and contributes to environmental damage through eutrophication, soil acidification and air pollution. Improving crop nutrient efficiency can improve agricultural sustainability by increasing yield while decreasing input costs and harmful environmental effects. Here, we review the mechanisms of nutrient efficiency (primarily for nitrogen, phosphorus, potassium and iron) and breeding strategies for improving this trait, along with the role of regulation of gene expression in enhancing crop nutrient efficiency to increase yields. We focus on the importance of root system architecture to improve nutrient acquisition efficiency, as well as the contributions of mineral translocation, remobilization and metabolic efficiency to nutrient utilization efficiency.

Abstract (Browse 129)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
          Cell and Developmental Biology
ARR12 promotes de novo shoot regeneration in Arabidopsis thaliana via activation of WUSCHEL expression
Author: Xuehuan Dai, Zhenhua Liu, Meng Qiao, Juan Li, Shuo Li and Fengning Xiang
Journal of Integrative Plant Biology 1900 待分类(待分类): 1-13
Published Online: July 5, 2017
DOI: 10.1111/jipb.12567

Auxin and cytokinin direct cell proliferation and differentiation during the in vitro culture of plant cells, but the molecular basis of these processes, especially de novo shoot regeneration, has not been fully elucidated. Here, we describe the regulatory control of shoot regeneration in Arabidopsis thaliana (L.) Heynh, based on the interaction of ARABIDOPSIS RESPONSE REGULATOR12 (ARR12) and WUSCHEL (WUS). The major site of ARR12 expression coincided with the location where the shoot apical meristem (SAM) initiated. The arr12 mutants showed severely impaired shoot regeneration and reduced responsiveness to cytokinin; consistent with this, the overexpression of ARR12 enhanced shoot regeneration. Certain shoot meristem specification genes, notably WUSCHEL (WUS) and CLAVATA3, were significantly downregulated in the arr12 explants. Chromatin immunoprecipitation (ChIP) and transient activation assays demonstrated that ARR12 binds to the promoter of WUS. These observations indicate that during shoot regeneration, in vitro, ARR12 functions as a molecular link between cytokinin signaling and the expression of shoot meristem specification genes.

Abstract (Browse 75)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
          Plant-environmental Interactions
Localized micronutrient patches induce lateral root foraging and chemotropism in Nicotiana attenuata
Author: Abigail P. Ferrieri, Ricardo A.R. Machado, Carla C.M. Arce, Danny Kessler, Ian T. Baldwin and Matthias Erb
Journal of Integrative Plant Biology 1900 待分类(待分类): 1-13
Published Online: June 26, 2017
DOI: 10.1111/jipb.12566

Nutrients are distributed unevenly in the soil. Phenotypic plasticity in root growth and proliferation may enable plants to cope with this variation and effectively forage for essential nutrients. However, how micronutrients shape root architecture of plants in their natural environments is poorly understood. We used a combination of field and laboratory-based assays to determine the capacity of Nicotiana attenuata to direct root growth towards localized nutrient patches in its native environment. Plants growing in nature displayed a particular root phenotype consisting of a single primary root and a few long, shallow lateral roots. Analysis of bulk soil surrounding the lateral roots revealed a strong positive correlation between lateral root placement and micronutrient gradients, including copper, iron and zinc. In laboratory assays, the application of localized micronutrient salts close to lateral root tips led to roots bending in the direction of copper and iron. This form of chemotropism was absent in ethylene and jasmonic acid deficient lines, suggesting that it is controlled in part by these two hormones. This work demonstrates that directed root growth underlies foraging behavior, and suggests that chemotropism and micronutrient-guided root placement are important factors that shape root architecture in nature.

Abstract (Browse 68)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       


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