July 2014, Volume 56 Issue 7, Pages 610每711.


Cover Caption: KATANIN in Phragmoplasts
About the cover: The morphology and size of phragmoplast, a novel plant cell-specific apparatus in cytokinesis, are strictly regulated. In this issue, Wan et al. (pp. 622每634) showed in KATANIN P80 over-expression rice plants that the phragmoplast is lengthened while the distance between two daughter nuclei is shortened. Consequently, root growth in those lines is retarded, suggesting an important role of KATANIN in cytokinesis.

 

          New Technology
Performance index: An expeditious tool to screen for improved drought resistance in the Lathyrus genus
Author: Susana Silvestre, Susana de Sousa Araújo, Maria Carlota Vaz Patto and Jorge Marques da Silva
Journal of Integrative Plant Biology 2014 56(7): 610每621
Published Online: February 20, 2014
DOI: 10.1111/jipb.12186
      
    

Some species of the Lathyrus genus are among the most promising crops for marginal lands, with high resilience to drought, flood, and fungal diseases, combined with high yields and seed nutritional value. However, lack of knowledge on the mechanisms underlying its outstanding performance and methodologies to identify elite genotypes has hampered its proper use in breeding. Chlorophyll a fast fluorescence transient (JIP test), was used to evaluate water deficit (WD) resistance in Lathyrus genus. Our results reveal unaltered photochemical values for all studied genotypes showing resistance to mild WD. Under severe WD, two Lathyrus sativus genotypes showed remarkable resilience maintaining the photochemical efficiency, contrary to other genotypes studied. Performance index (PIABS) is the best parameter to screen genotypes with improved performance and grain production under WD. Moreover, we found that JIP indices are good indicators of genotypic grain production under WD. Quantum yield of electron transport (ϕEo) and efficiency with which trapped excitons can move electrons further than QA (ψ0) revealed as important traits related to improved photosynthetic performance and should be exploited in future Lathyrus germplasm improvements. The JIP test herein described showed to be an expeditious tool to screen and to identify elite genotypes with improved drought resistance.

 

Silvestre S, Araújo SS, Vaz Patto MC, Marques da Silva J (2014) Performance index: An expeditious tool to screen for improved drought resistance in the Lathyrus genus. J Integr Plant Biol 56: 610–621. doi: 10.1111/jipb.12186

Abstract (Browse 959)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
          Cell and Developmental Biology
Overexpression of OsKTN80a, a katanin P80 ortholog, caused the repressed cell elongation and stalled cell division mediated by microtubule apparatus defects in primary root in Oryza sativa
Author: Lei Wan, Xiuwen Wang, Shaoqing Li, Jun Hu, Wenchao Huang and Yingguo Zhu
Journal of Integrative Plant Biology 2014 56(7): 622每634
Published Online: January 23, 2014
DOI: 10.1111/jipb.12170
      
    

Katanin, a microtubule-severing enzyme, consists of two subunits: the catalytic subunit P60, and the regulatory subunit P80. In several species, P80 functions in meiotic spindle organization, the flagella biogenesis, the neuronal development, and the male gamete production. However, the P80 function in higher plants remains elusive. In this study, we found that there are three katanin P80 orthologs (OsKTN80a, OsKTN80b, and OsKTN80c) in Oryza sativa L. Overexpression of OsKTN80a caused the retarded root growth of rice seedlings. Further investigation indicates that the retained root growth was caused by the repressed cell elongation in the elongation zone and the stalled cytokinesis in the division zone in the root tip. The in vivo examination suggests that OsKTN80a acts as a microtubule stabilizer. We prove that OsKTN80a, possibly associated with OsKTN60, is involved in root growth via regulating the cell elongation and division.

 

Wan L, Wang X, Li S, Hu J, Huang W, Zhu Y (2014) Overexpression of OsKTN80a, a katanin P80 ortholog, caused the repressed cell elongation and stalled cell division mediated by microtubule apparatus defects in primary root in Oryza sativa. J Integr Plant Biol 56: 622–634. doi: 10.1111/jipb.12170

Abstract (Browse 1142)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
An auxin-responsive endogenous peptide regulates root development in Arabidopsis  
Author: Fengxi Yang, Yu Song, Hao Yang, Zhibin Liu, Genfa Zhu and Yi Yang
Journal of Integrative Plant Biology 2014 56(7): 635每647
Published Online: January 30, 2014
DOI: 10.1111/jipb.12178
      
    

Auxin plays critical roles in root formation and development. The components involved in this process, however, are not well understood. Here, we newly identified a peptide encoding gene, auxin-responsive endogenous polypeptide 1 (AREP1), which is induced by auxin, and mediates root development in Arabidopsis. Expression of AREP1 was specific to the cotyledon and to root and shoot meristem tissues. Amounts of AREP1 transcripts and AREP1-green fluorescent protein fusion proteins were elevated in response to indoleacetic acid treatment. Suppression of AREP1 through RNAi silencing resulted in reduction of primary root length, increase of lateral root number, and expansion of adventitious roots, compared to the observations in wild-type plants in the presence of auxin. By contrast, transgenic plants overexpressing AREP1 showed enhanced growth of the primary root under auxin treatment. Additionally, root morphology, including lateral root number and adventitious roots, differed greatly between transgenic and wild-type plants. Further analysis indicated that the expression of auxin-responsive genes, such as IAA3, IAA7, IAA17, GH3.2, GH3.3, and SAUR-AC1, was significantly higher in AREP1 RNAi plants, and was slightly lower in AREP1 overexpressing plants than in wild-type plants. These results suggest that the novel endogenous peptide AREP1 plays an important role in the process of auxin-mediated root development.

 

Yang F, Song Y, Yang H, Liu Z, Zhu G, Yang Y (2014) An auxin坼responsive endogenous peptide regulates root development in Arabidopsis. J Integr Plant Biol 56: 635–647. doi: 10.1111/jipb.12178

Abstract (Browse 1372)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
          Molecular Ecology and Evolution
Evolutionary dynamics of leucine-rich repeat receptor-like kinases and related genes in plants: A phylogenomic approach  
Author: Tao Shi, Hongwen Huang, Michael J Sanderson and Frans E Tax
Journal of Integrative Plant Biology 2014 56(7): 648每662
Published Online: February 24, 2014
DOI: 10.1111/jipb.12188
      
    
Leucine-rich repeat (LRR) receptor-like kinases (RLKs), evolutionarily related LRR receptor-like proteins (RLPs) and receptor-like cytoplasmic kinases (RLCKs) have important roles in plant signaling, and their gene subfamilies are large with a complicated history of gene duplication and loss. In three pairs of closely related lineages, including Arabidopsis thaliana and A. lyrata (Arabidopsis), Lotus japonicus, and Medicago truncatula (Legumes), Oryza sativa ssp. japonica, and O. sativa ssp. indica (Rice), we find that LRR RLKs comprise the largest group of these LRR-related subfamilies, while the related RLCKs represent the smallest group. In addition, comparison of orthologs indicates a high frequency of reciprocal gene loss of the LRR RLK/LRR RLP/RLCK subfamilies. Furthermore, pairwise comparisons show that reciprocal gene loss is often associated with lineage-specific duplication(s) in the alternative lineage. Last, analysis of genes in A. thaliana involved in development revealed that most are highly conserved orthologs without species-specific duplication in the two Arabidopsis species and originated from older Arabidopsis-specific or rosid-specific duplications. We discuss potential pitfalls related to functional prediction for genes that have undergone frequent turnover (duplications, losses, and domain architecture changes), and conclude that prediction based on phylogenetic relationships will likely outperform that based on sequence similarity alone.
 

Shi T, Huang H, Sanderson MJ, Tax FE (2014) Evolutionary dynamics of leucine坼rich repeat receptor坼like kinases and related genes in plants: A phylogenomic approach. J Integr Plant Biol 56: 648–662. doi: 10.1111/jipb.12188

Abstract (Browse 1031)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
          Plant-environmental Interactions
Identification and characterization of genes involved in the jasmonate biosynthetic and signaling pathways in mulberry (Morus notabilis)
Author: Qing Wang, Bi Ma, Xiwu Qi, Qing Guo, Xuwei Wang, Qiwei Zeng and Ningjia He
Journal of Integrative Plant Biology 2014 56(7): 663每672
Published Online: January 16, 2014
DOI: 10.1111/jipb.12166
      
    

Jasmonate (JA) is an important phytohormone regulating growth, development, and environmental response in plants, particularly defense response against herbivorous insects. Recently, completion of the draft genome of the mulberry (Morus notabilis) in conjunction with genome sequencing of silkworm (Bombyx mori) provides an opportunity to study this unique plant-herbivore interaction. Here, we identified genes involved in JA biosynthetic and signaling pathways in the genome of mulberry for the first time, with the majority of samples showing a tissue-biased expression pattern. The analysis of the representative genes 12-oxophytodienoic acid reductase (OPRs) and jasmonate ZIM-domain (JAZs) was performed and the results indicated that the mulberry genome contains a relatively small number of JA biosynthetic and signaling pathway genes. A gene encoding an important repressor, MnNINJA, was identified as an alternative splicing variant lacking an ethylene-responsive element binding factor-associated amphiphilic repression motif. Having this fundamental information will facilitate future functional study of JA-related genes pertaining to mulberry-silkworm interactions.

 

Wang Q, Ma B, Qi X, Guo Q, Wang X, Zeng Q, He N (2014) Identification and characterization of genes involved in the jasmonate biosynthetic and signaling pathways in mulberry (Morus notabilis). J Integr Plant Biol 56: 663–672. doi: 10.1111/jipb.12166

Abstract (Browse 1128)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
Traits associated with winter wheat grain yield in Central and West Asia
Author: Marta Silva Lopes, Didem Saglam, Mutlu Ozdogan and Matthew Reynolds
Journal of Integrative Plant Biology 2014 56(7): 673每683
Published Online: January 24, 2014
DOI: 10.1111/jipb.12172
      
    
Improved adaptation of winter wheat (Triticum aestivum L.) to drought and heat may be influenced by days to heading, plant height, biomass, canopy temperature (CT) at grain filling, and rate of senescence. This study shows that, under supplemental irrigation or rainfed conditions, days to heading and plant height together explain up to 68% of grain yield (GY) variation, and these associations were further confirmed in several locations across West and Central Asia. Days to heading can be slightly reduced below that of check line Karahan to further improve GY while avoiding the effect of late frosts. Plant height has been decreased in recent germplasm, but further reductions below that of check line Karahan could still improve GY in a wide range of environments. However, in Iranian sites, taller genotypes showed better adaptation with higher biomass and increased reserves for grain filling. Canopy temperature and rate senescence were not associated with GY. A normalized difference vegetation index, used to estimate biomass (Feekes stages 4–5), had intermediate heritability across environments and correlated positively with GY under low plant density and should be explored further as a tool for early selection.

 

Lopes MS, Saglam D, Ozdogan M, Reynolds M (2014) Traits associated with winter wheat grain yield in Central and West Asia. J Integr Plant Biol 56: 673–683. doi: 10.1111/jipb.12172

Abstract (Browse 790)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
AtPEPTIDE RECEPTOR2 mediates the AtPEPTIDE1-induced cytosolic Ca2+ rise, which is required for the suppression of Glutamine Dumper gene expression in Arabidopsis roots
Author: Chunli Ma, Jie Guo, Yan Kang, Kohei Doman, Anthony C. Bryan, Frans E. Tax, Yube Yamaguchi and Zhi Qi
Journal of Integrative Plant Biology 2014 56(7): 684每694
Published Online: January 23, 2014
DOI: 10.1111/jipb.12171
      
    

AtPEPTIDE RECEPTOR2 (AtPEPR2) is a member of leucine-rich repeat receptor-like kinase family and binds to a group of AtPROPEP gene-encoded endogenous peptides, AtPeps. Previously, we found that AtPEPR2 plays a moderate role in the AtPep1-mediated innate immunity responses in Arabidopsis leaf. In this study, we found that AtPEPR2 promoter has strong activity in the vascular tissues of the roots and the atpepr2 mutants showed a moderate but significantly shorter root phenotype. AtPEPR2 partially mediated AtPep1-induced root elongation inhibition. AtPep1-triggered cytosolic Ca2+ transient rise in roots showed partial dependence on AtPEPR2 and fully on extracellular Ca2+ ([Ca2+]ext). Transcriptional profiling analysis found that expression of 75% of AtPep1-modulated genes in roots was fully dependent on AtPEPR2, of which two dramatically induced genes showed partial dependence on the [Ca2+]ext. Arabidopsis genome contains seven Glutamine Dumpers genes (AtGDUs), encoding amino acid exporters. Three of them (AtGDU2, 3, 5) were among the top 10 genes that were downregulated by AtPep1 through AtPEPR2 fully dependent pathway. Treatment with AtPep1 strongly suppressed promoter activity of AtGDU3 in roots, which was relieved by chelating [Ca2+]ext. Arabidopsis overexpressing AtGDU3 showed a shorter root phenotype and decreased sensitivity to the AtPep1-mediated inhibition of root elongation. Taken together, this study demonstrated a significant role of AtPEPR2 in the AtPep1-mediated signaling in the roots.

 

Ma C, Guo J, Kang Y, Doman K, Bryan AC, Tax FE, Yamaguchi Y, Qi Z (2014) AtPEPTIDE RECEPTOR2 mediates the AtPEPTIDE1坼induced cytosolic Ca2+ rise, which is required for the suppression of Glutamine Dumper gene expression in Arabidopsis roots. J Integr Plant Biol 56: 684–694. doi: 10.1111/jipb.12171

Abstract (Browse 994)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
Quantitative genetic analysis of chlorophyll a fluorescence parameters in maize in the field environments
Author: Domagoj Šimić, Hrvoje Lepeduš, Vlatka Jurković, Jasenka Antunović and Vera Cesar
Journal of Integrative Plant Biology 2014 56(7): 695每708
Published Online: February 13, 2014
DOI: 10.1111/jipb.12179
      
    

Chlorophyll fluorescence transient from initial to maximum fluorescence (“P” step) throughout two intermediate steps (“J” and “I”) (JIP-test) is considered a reliable early quantitative indicator of stress in plants. The JIP-test is particularly useful for crop plants when applied in variable field environments. The aim of the present study was to conduct a quantitative trait loci (QTL) analysis for nine JIP-test parameters in maize during flowering in four field environments differing in weather conditions. QTL analysis and identification of putative candidate genes might help to explain the genetic relationship between photosynthesis and different field scenarios in maize plants. The JIP-test parameters were analyzed in the intermated B73 × Mo17 (IBM) maize population of 205 recombinant inbred lines. A set of 2,178 molecular markers across the whole maize genome was used for QTL analysis revealing 10 significant QTLs for seven JIP-test parameters, of which five were co-localized when combined over the four environments indicating polygenic inheritance and pleiotropy. Our results demonstrate that QTL analysis of chlorophyll fluorescence parameters was capable of detecting one pleiotropic locus on chromosome 7, coinciding with the gene gst23 that may be associated with efficient photosynthesis under different field scenarios.

 

Šimic D, Lepeduš H, Jurkovic V, Antunovic J, Cesar V (2014) Quantitative genetic analysis of chlorophyll a fluorescence parameters in maize in the field environments. J Integr Plant Biol 56: 695–708. doi: 10.1111/jipb.12179

Abstract (Browse 822)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
Corrigendum
Author:
Journal of Integrative Plant Biology 2014 56(7): 709每709
Published Online: July 4, 2014
DOI: 10.1111/jipb.12223
Abstract (Browse 375)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
          Obituary
In memory of Professor Ping Wu (1957每2014)  
Author: Guohua Xu and Dong Liu
Journal of Integrative Plant Biology 2014 56(7): 710每711
Published Online: July 4, 2014
DOI: 10.1111/jipb.12232
Abstract (Browse 499)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
 

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