March 2018, Volume 60 Issue 3, Pages 189每261.


Cover Caption: Diversity of peanut genetic resources
Association analysis may provide resource for marker-assisted selection and facilitate peanut breeding. In this issue, Zhang et al. (pp. 195-215) established a peanut association panel with extensive phenotypic and genetic variations. Preliminary analysis allowed them to detect markers related to seed quality and a set of alleles with positive and negative effects.

 

          Letter to the Editor
Jasmonate signaling makes flowers attractive to pollinators and repellant to florivores in nature  
Author: Ran Li, Meredith C. Schuman, Yang Wang, Lucas Cortés Llorca, Julia Bing, Anne Bennion, Rayko Halitschke and Ian T. Baldwin
Journal of Integrative Plant Biology 2018 60(3): 190每194
Published Online: October 23, 2017
DOI: 10.1111/jipb.12607
      
    

Flowers are required for the Darwinian fitness of flowering plants, but flowers’ advertisements for pollination services can attract florivores. Previous glasshouse work with Nicotiana attenuata revealed the role of jasmonate (JA) signaling in flower development, advertisement and defense. However, whether JA signaling mediates flowers’ filtering of floral visitors in nature remained unknown. This field study revealed that silencing JA signaling resulted in flowers that produce less nectar and benzyl acetone, two pollinator-attractive traits. Meanwhile, flowers of defenseless plants were highly attacked by a suite of native herbivores, and damage to buds in native plants correlated negatively with their JA-Ile levels.

Abstract (Browse 399)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
Flowers advertise for pollinator services with nectar, scent and pigments. These traits also make flowers apparent to florivores and nectar robbers. Our field studies reveal how plants use a universal phytohormone signaling pathway to solve a flower's dilemma that advertisements for pollinator services is perceived by mutualists and antagonists alike.
          New Resources
Establishment and evaluation of a peanut association panel and analysis of key nutritional traits  
Author: Xiurong Zhang, Suqing Zhu, Kun Zhang, Yongshan Wan, Fengzhen Liu, Qingfang Sun and Yingjie Li
Journal of Integrative Plant Biology 2018 60(3): 195每215
Published Online: October 4, 2017
DOI: 10.1111/jipb.12601
      
    

Breeding programs aim to improve the yield and quality of peanut (Arachis hypogaea L.); using association mapping to identify genetic markers linked to these quantitative traits could facilitate selection efficiency. A peanut association panel was established consisting of 268 lines with extensive phenotypic and genetic variation, meeting the requirements for association analysis. These lines were grown over 3 years and the key agronomic traits, including protein and oil content were examined. Population structure (Q) analysis showed two subpopulations and clustering analysis was consistent with Q-based membership assignment and closely related to botanical type. Relative Kinship (K) indicated that most of the panel members have no or weak familial relatedness, with 52.78% of lines showing K = 0. Linkage disequilibrium (LD) analysis showed a high level of LD occurs in the panel. Model comparisons indicated false positives can be effectively controlled by taking Q and K into consideration and more false positives were generated by K than Q. A preliminary association analysis using a Q + K model found markers significantly associated with oil, protein, oleic acid, and linoleic acid, and identified a set of alleles with positive and negative effects. These results show that this panel is suitable for association analysis, providing a resource for marker-assisted selection for peanut improvement.

Abstract (Browse 1068)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
The association mapping approach could facilitate marker-assisted selection and breeding improvement. We have established a peanut association panel with extensive phenotypic and genetic variations for further association analysis, detected candidate markers related to seed quality, and identified a set of alleles with positive and negative effects.
          Molecular Physiology
Characterization of the soybean GmALMT family genes and the function of GmALMT5 in response to phosphate starvation
Author: Wenting Peng, Weiwei Wu, Junchu Peng, Jiaojiao Li, Yan Lin, Yanan Wang, Jiang Tian, Lili Sun, Cuiyue Liang and Hong Liao
Journal of Integrative Plant Biology 2018 60(3): 216每231
Published Online: October 17, 2017
DOI: 10.1111/jipb.12604
      
    

A potential mechanism to enhance utilization of sparingly soluble forms of phosphorus (P) is the root secretion of malate, which is mainly mediated by the ALMT gene family in plants. In this study, a total of 34 GmALMT genes were identified in the soybean genome. Expression patterns diverged considerably among GmALMTs in response to phosphate (Pi) starvation in leaves, roots and flowers, with expression altered by P availability in 26 of the 34 GmALMTs. One root-specific GmALMT whose expression was significantly enhanced by Pi-starvation, GmALMT5, was studied in more detail to determine its possible role in soybean P nutrition. Analysis of GmALMT5 tissue expression patterns, subcellular localization, and malate exudation from transgenic soybean hairy roots overexpressing GmALMT5, demonstrated that GmALMT5 is a plasma membrane protein that mediates malate efflux from roots. Furthermore, both growth and P content of transgenic Arabidopsis overexpressing GmALMT5 were significantly increased when sparingly soluble Ca-P was used as the external P source. Taken together, these results indicate that members of the soybean GmALMT gene family exhibit diverse responses to Pi starvation. One member of this family, GmALMT5, might contribute to soybean P efficiency by enhancing utilization of sparingly soluble P sources under P limited conditions.

Abstract (Browse 272)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
A potential mechanism to enhance utilization of sparingly soluble forms of phosphorus (P) is the root secretion of organic acid. Soybean root malate exudation mediated by GmALMT5 might contribute to soybean P efficiency by enhancing utilization of sparingly soluble P sources under P limited conditions.
Quantitative trait loci associated with soybean seed weight and composition under different phosphorus levels
Author: Gokhan Hacisalihoglu, Amy L. Burton, Jeffery L. Gustin, Selim Eker, Safiye Asikli, Elif Hakli Heybet, Levent Ozturk, Ismail Cakmak, Atilla Yazici, Kent O. Burkey, James Orf and A. Mark Settles
Journal of Integrative Plant Biology 2018 60(3): 232每241
Published Online: November 13, 2017
DOI: 10.1111/jipb.12612
      
    

Seed size and composition are important traits in food crops and can be affected by nutrient availability in the soil. Phosphorus (P) is a non-renewable, essential macronutrient, and P deficiency limits soybean (Glycine max) yield and quality. To investigate the associations of seed traits in low- and high-P environments, soybean recombinant inbred lines (RILs) from a cross of cultivars Fiskeby III and Mandarin (Ottawa) were grown under contrasting P availability environments. Traits including individual seed weight, seed number, and intact mature pod weight were significantly affected by soil P levels and showed transgressive segregation among the RILs. Surprisingly, P treatments did not affect seed composition or weight, suggesting that soybean maintains sufficient P in seeds even in low-P soil. Quantitative trait loci (QTLs) were detected for seed weight, intact pods, seed volume, and seed protein, with five significant QTLs identified in low-P environments and one significant QTL found in the optimal-P environment. Broad-sense heritability estimates were 0.78 (individual seed weight), 0.90 (seed protein), 0.34 (seed oil), and 0.98 (seed number). The QTLs identified under low P point to genetic regions that may be useful to improve soybean performance under limiting P conditions.

Abstract (Browse 274)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
Can seed size and composition traits be affected by soil nutrient availability? We mapped quantitative trait loci for protein, volume, and seed weight that provide potential targets for improvement and benefit of soybean (Glycine max) through breeding. We report that low P reduced yield but minimally influenced composition or size.
          Plant-abiotic Interactions
Nicotiana attenuata's capacity to interact with arbuscular mycorrhiza alters its competitive ability and elicits major changes in the leaf transcriptome  
Author: Ming Wang, Julia Wilde, Ian T. Baldwin and Karin Groten
Journal of Integrative Plant Biology 2018 60(3): 242每261
Published Online: October 31, 2017
DOI: 10.1111/jipb.12609
      
    

To study the local and systemic effects of arbuscular mycorrhizal fungal (AMF) colonization, Nicotiana attenuata plants impaired in their interactions with AMF due to silencing of a calcium- and calmodulin dependent protein kinase (inverted repreat (ir)CCaMK) were grown competitively in pairs with empty vector (EV) plants, with and without two different types of inoculum. When inoculated, EV plants strongly outperformed irCCaMK plants. Foliar transcript profiling revealed that AMF colonization significantly changed gene expression of P-starvation and -transporter genes in irCCaMK plants. The Pht1 family phosphate transporter NaPT5 was not only specifically induced in roots after AMF colonization, but also in leaves of AMF-colonized irCCaMK plants, and in plants grown under low Pi conditions in the absence of AMF. The P-starvation signature of inoculated irCCaMK plants corresponded with increases in selected amino acids and phenolic compounds in leaves. We also found a strong AMF-induced increase in amino acids and phenolic metabolites in roots. Plants impaired in their interactions with AMF clearly have a fitness disadvantage when competing for limited soil nutrients with a fully functional isogenic line. The additional role of the AMF-induced Pht1 family transporter NaPT5 in leaves under P-starvation conditions will require further experiments to fully resolve.

Abstract (Browse 852)  |  References  |  Full Text HTML  |  Full Text PDF  |  Cited By       
Plants impaired in their interactions with arbuscular mycorrhiza due to the silencing of a calcium and calmodulin dependent protein kinase (CCaMK) have a fitness disadvantage when competing for limited soil nutrients with a fully functional isogenic line and display the transcriptional signatures of P starvation in their leaves, as revealed by foliar transcriptome and metabolite analysis of competitively grown plants.
 
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