February 1973, Volume 15 Issue 2


          Research Articles
A Study on the Seed Dormancy in Torreya grandis
Author: Shin Chung-li and Chao Tung-fang
Journal of Integrative Plant Biology 1973 15(2)
Abstract (Browse 1786)  |  Full Text PDF       
A Preliminary Observations: Effect of Different Ages Enviromental Conditions of the Needle Structure of Pinus elliottii
Author: Chuang Foo-chui
Journal of Integrative Plant Biology 1973 15(2)
Abstract (Browse 1657)  |  Full Text PDF       
The Morphology of Archangiopteris Christ et Gies. and Its Reletionship with Angiopteris Hoffm.
Author: Chang Chih-y邦
Journal of Integrative Plant Biology 1973 15(2)
    The present paper is devoted to a study of the basic morphological and anatomicalcharacteristics of the endemic Sino-Vietnamese genus Archangiopteris Christ et Gies. The result of the study has shown that Archangiopteris differs from Angiopteris iioffm in a number of important features. Morphologically, it is distinguished from Angiopteris by rather long-creeping, dorsiventral rhizome with distichous leaves(fronds), which are, as a rule, simply impari-pinnate, with usually 1每5 pairs of lateral pinnae; the fleshy stipe being provided with 1 or rarely 4每5 geniculate swellingsat the middle and clad in the lower part in coarsely dentate thin scales; the linear soriconsisting of 60每260 (sometimes 20 in the bipinnate-leaved species) sporangia,situated midway between the costa and leaf margin and mingled from underneathwith branched hair-like malticelinlar paraphyses; and by a spore-output per sporan-glum of 1800每1900 spores which are sphaerotetraedric, translucent, with finelyechinose exospores. Anatomically, Archangiopteris, unlike Angiopteris, has a dictyos-tele consisting of one single vascular cylinder, also dorsiventral in structure., withsingle leaf-trace from its dorsal side and root trace from its ventral side, and the protoxylem elements are endarch both in the rhizome and the petiole. The results of comparison indicated, however, that Archangiopteris is closelyrelated to Angiopteris. It is quite possible that the genus is derived from Angiopterisby way of such simply pinnate-leaved species of Angiopteris as A. pinnata Ching, to Archangiopteris cadicri Tard-Blot et C. Chr. The morphological and anatomical features as indicated above tend to show that the genus is more advanced than Angiopteris. The characteristic fleshy no dose swelling of the stipe in Archangiopteris has been also anatomically studied. It has been considered as a structure of an adaptive nature in response to its feebly lighted habitat, whereby controlling the orientation of fronds in relation to the direction and intensity of the sun's rays and thus enabling the plant to absorb enough light energy for photosynthesis. The problem of the similarity in the external morphology and internal structure of Archangiopteris to the sporeling of Angiopteris has been also studied both in the field and in the laboratory. This phenomenon has been explained as an example of what is known as juvenile maturity in the plant life (neoteny). Archangiopteris, when first established as a new genus by Christ and Giesenhagenin 1899, was considered as a primitive fern genus of Marattiales, a view, which the present study of the genus does not prove to be correct, for, although Archangiopteris,in contrast with Angiopteris, has a much more simple external morphology and internalstructure, this simplification mw not necessarily reflect the primitiveness of the genus, but perhaps rather as a result of juvenile maturity. It may be assumed thatin the course of evolution through great geological ages, the genus in adapting itselfto the particular environment has developed a series of advanced characters as mentioned at the outset. In his study of taxonomy of the genus Archangiopteris, R. C. Ching (1958) pointed out that the genus was a direct derivative from Angiopteris, aview which agrees well with the result of the observations by the present writer.
Abstract (Browse 2067)  |  Full Text PDF       
On the Discovery of Some Plant Fossils from the Mt. Jolmo-Lungma Region, Southern Tibet, and Its Significance
Author: Hs邦 Jen
Journal of Integrative Plant Biology 1973 15(2)
    Plant fossils, Glossopteris communis Feistm., Sphenopteris cf. hughesii (Feistm.) Arber and Pecopteris sp., are recorded from the district Ding-Ri, southern Tibet, at the locality WSluluo, about 30 km north to the Mount Jolmo Lungma of central Himalayas. The plant-bearing beds are correlated with the Raniganj Beds of the Damuda Division of South Asia. The geological age is assigned to early late Permian. The present paper supports the view claimed by B. Sahni (1938) that the northern coastline of the old Gondwana continent was roughly parallel to the. Main Himalayan arc and it was in this region up heaved with the late Tertiary mountain building movements.
Abstract (Browse 1772)  |  Full Text PDF       
On the Eco-geographical Characters and the Problems of Classification of the Wild Fruit-tree Forest in the Ili Valley of Sinkiang
Author: Chang Hsin-shi
Journal of Integrative Plant Biology 1973 15(2)
    The wild fruit-tree forest which is distributed in the Ili Valley of the Tien-Shan Mountains in western Sinkiang, consists of Tien-shan apple (Malus sieversii), wildapricot (Armeniaca vulgaris) and wild walnut (Juglans regia), etc. It is a broad-leaved forest type with "oceanic" climate, which appears in the mountain region of desert zone. Its distribution is closely related to the complex of special ecologicalfactors, such as abundant rainfall of the locality, evident "inversion" in winter, and relief elements in which the cold air current is evaded, etc. The present forest is a valuable "relict" in the mountain region. It is a paleo-geographical phenomenon-the result in which the broad-leaved forest elements of Tertiary paleotemperate zone combine with elements of the boreal forest and meadow. It is evidently distinguished in geographical elements from the fruit-tree forest in the western Tien-shan Mountains of Middle Asia. The classification of wild fruit-tree forest in the Ill Valley is as follows: 1. Tien-shan apple and wild apricot-Tien-shan apple Formation: (1) Malus sieversii-Bromus benekenii Ass. (2) M. sieversii-Brachypodium silvaticum Ass. (3) M. sieversii-Impatiens brachycentra Ass. (4) M. sieversii+Populus tremula-Aegopodium alpestre Ass. (5) M. sieversii-Shrubs-grasses Ass. in valley. (6) M. sieversii + Armeniaca vulgaris-shrubs-grasses Ass. (7) M. sieversii+A, vulgaris-Spiraea hypericifolia-steppe grasses Ass. 2. Wild walnut Formation: (1) Juglans regia-Brachypodium silvaticum + herbs Ass. (2) J. regia-Impatiens braehycentra+Aegopodium podagraria Ass.Sparse wild apricot Formation.
Abstract (Browse 2165)  |  Full Text PDF       
Phenomena of Protoplasmic Withdrawal in Withering Leaves of Higher Plants and the Possible Role of Protoplasmic Movement in Translocation
Author: Lou Chen-hou, Shao Li-mei and Duan Ching-hsia
Journal of Integrative Plant Biology 1973 15(2)
    Ordinarily, translocation in higher plants is mainly concerned with sugar transport in sieve tubes. As the protoplasm retains an appreciable amount of complexproteins in corporating various essential mineral elements, exhaustive evacuation ofcellular content in withering leaves is an important aspect of translocation worthy ofserious consideration. It is not only for its theoretical implications in connection withother aspects of translocation, but also for its significant role in crop productivity. Improvement in yield and quality of cereal grains at the senescence stage of the cropdepends largely upon whether the plant is killed untimely by early frost before exhaustive evacuation of cellular content from withering leaves is completed. The present investigation is a continuation of our previous works and is engaged in asystematic investigation of the sequence of events taking place in partial disintegration and exhaustive evacuation of protoplasm in withering leaf sheaths of Allium andwheat, by employing the easily stainable and recognizable chromatic substance of the nucleus as the main indicator. The. experimental results reaffirm our early proposal that exhaustive evacuation of cellular content in withering leaves is only rendered possible by intercellular movement of protoplasm itself after partial disintegration. Evacuation starts from the migration of the protoplasm into the leaf vein and continues its course in the conducting channel. Frequently, nuclear material may evenintrude into the vessel due to overcrowding. The most conspicuous indication of the partial disintegration of protoplasm is the dissolution of nucleus and the release of its constituents. The released chromatic substance gradually changes its form during it smigration toward the vascular bundle. Appreciable deposition of calcium salt crystalsin various forms which usually appears in the otherwise emptied cells of completelysenescent leaves seems to be the debris left over by decalcification during protoplasmic disintegration. Not only is the transport of cellular content in senescent tissues carried out byinter cellular movement of protoplasm, our early work has shown that such intercellular movement also occurs in the tender growing zone. Intercellular movement of protoplasm in higher plants should be considered a kind of cellular movement inmorphogenesis which is a common phenomenon in animal embryology as well as in slime molds, as the cellular morphogenetic movement in mycellium of higher fungiand in ordinary tissues of higher plants must necessarily traverse through the narrow channels connecting the rigid cellulose compartments. It seems to us the only plausible and effective way of complete withdrawal of cellular content from the deteriorating organ and its subsequent migration into the newly grown is to rely upon inter-cellular movement of protoplasm. On the other hand, storage food such as starchmust first degrade into water-soluble form so as to conform itself to the mass flow of sap. Accordingly, a dual performance of phloem transport is proposed here with: both rapid mass flow of sap and slow protoplasmic migration can be taking placesimultaneously in the phloem. According to evidences presented in recent literatureby Kamiya, Pollard, and others, the various modes of protoplasmic movement inliving cells, whether it be the movement of the whole protoplasm, or the streaming of hyaloplasm within the ectoplasm, are probably ※propelled§ by contractile protein fibrils possessing ATPase activity. Consequently, both types of phloem transport can bedriven by contractile protein fibrils as well if they are under proper patterns of organization in the protoplasm. As to how one mode of action, the export of photosynthates from a normal leaf, is switched over to partial disintegration and evacuation of protoplasm, the key seems to be the againg of leaves which eventually reaches an irreversible state of senescenceand is possibly associated with the appearance of nuclear dissolution.
Abstract (Browse 2179)  |  Full Text PDF       
The Effect of Gibberellin on the Growth and Development of Ramie (Boehmeria nivea) as well as the Quality of Its Fibers
Author: Wu Hsian-jong
Journal of Integrative Plant Biology 1973 15(2)
    The present experiment showed that gibberellin applied on the 20 days seedlings could accelerate the growth rate of its stem, and consequently the yield of the fibers was increased. The promotion of the growth of the stem is not due to theincrement of the number of internodes, but is chiefly due to the elongation of the irinternodes. The accumulation of dry matter of the internodes was increased also. If the fertility of the soil is poor, the treatment of ramie with gibberellin mayinduce the decrease of the fresh weight of leaves and chlorosis, the chlorophyll content is lowered. But if the soil is rich in nitrogen fertilizers, gibberellin may promotethe growth of leaves and the synthesis of chlorophyll. The content of cellulose and protein in the leaves is increased also. Gibberellin is able to inhibit the development of ramie, so the earlier floweringof the plant is prevented, the flowers and fruits are decreased evidently. The weightof the seeds and the percentage of gemination are lower than those of the control. Because the stem has acquired full nourishment, the vegetative growth is enhanced, and in turn this favoured the formation of fibers in the stem. If the application of gibberellin is accompanied by short-day illumination, the effect will be more evident than at long-day illumination. Through the fiber determination, it was shown that the treatment of ramie withgibberellin was able to promote the length and elasticity of the fibers, and the incrementof the thickness of the fibrous cells was not so evident as the increment of their length.
Abstract (Browse 1881)  |  Full Text PDF       
The Occurrence of Some Important Tropane Alkaloids in Chinese Solanaceous Plants
Author: Hsiao Pei-ken, Hsia Kuang-cheng and Ho Li-yi
Journal of Integrative Plant Biology 1973 15(2)
    Hyoscyamine, scopolamine, anisodamine, anisodine are important tropane alkaloidsbecause of their therapeutic value and pharmacological actions. In recent years, thelatter two new tropane alkaloids have also been proved to affect the central or peripheral nerve system. This discovery has prompted further investigation into solanaceous plants with the hope of finding additional sources with higher content of the sealkaloids. The present paper studied the general pattern of distribution in the Chinesesolanaceous plants, of the above mentioned four tropane alkaloids and a non-tropanealkaloid, bellaradine, which may be biogenetically related to the tropane alkaloids. The variation of these alkaloids within a certain population belonging to Scopoliatangutica was studied as well. By means of TLC, 91 samples representing 19 genera and 54 species of the Chinese solanaceous plants were examined for the presence of hyoscyamine, scopolamine, anisodamine, anisodine and bellaradine. The results (Tab. 1) indicated thatthere is a high incidence of occurrence of such alkaloids in Trib. Solaneae, Subtrib.Hyoscyaminae, Subtrib. Mandragorinae and Trib. Datureae, but a notable absence in Trib. Nicandreae, Trib. Solaneae-Subtrib. Lyciinae, Subtrib. Solaninae and Trib. Cestreae (sensu Wettstein). The results pointed out that certain high-incidence so-lanaceous catagories may prove profitable for searching new sources of tropane alkaloids in the future. The hyoscyamine and anisodamine contents in Scopolia tangutica, Przewalskiashebbearei, P. tangutica, Physochlaina physaloides and Physochlaina infundibulumwere determined (Tab. 2). It was found that the roots of Przewalskia shebbeareiand P. tangutica showed a high content of hyoscyamine and anisodamine, amountingto 1.03%每1.65% and 0.12%每0.37%, respectively, in the former plant, and to 1.02% and0.11%每0.38%, respectively, in the latter. Therefore, Przewalskia shebbearei and P.tangutica would be noteworthy as new sources for these two alkaloids. In order to study the variation of chemical ingredients within a population of Scopolia tangutica, we selected 161 individuals at random to detect the presence of these five alkaloids (Tab. 3). The proportions of positive and negative results were: hyoscyamine 112:49, scopolamine 64:97, anisodamine 154:7, anisodine 31:130, bellaradine 135:26. These data revealed that the presence or absence of the five alkaloidsin a given population are not invariant. Furthermore, the alkaloid contents of hyoscyamine and anisodamine are also variable according to different localities (Tab. 4). The results may be of value to chemotaxonomy or systematics of Solanaceae.
Abstract (Browse 2082)  |  Full Text PDF       
A Primary Study on the Vertical Vegetation Belt of Mt. Jolmo-Lungma (Everest) Region and Its Relationship with Horizontal Zone
Author: Chang King-wai and Chiang Shu
Journal of Integrative Plant Biology 1973 15(2)
    Mt. Jolmo-Lungma (Everest) is the highest peak on the earth. The subdivision of vertical vegetation belt there has been reported previously. But between scientistsopinions about its relationship with horizontal vegetation zone are still debatable. This paper deal primary with the observations of the vertical vegetational belt of Mt.Jolmo-Lungma and discussed its nature as compared with the horizontal vegetationzone. The climate of Mt. Jolmo-Lungma region characterized by its intensive solar-radiation, rich sunlight, great diurnal but small annual variation of temperature and higher precipition in the summer. Owing to the great height of the Tibetan plateau and the Himalaya range which intercapts the warm and wet monsoon of Indian Ocean from the south and at the same time hindered the cold currents from the north, there has, naturally, developed a difference of climate and vegetation on either side of Mt. Johno-Lungma. There are more rainfalls on the southern slope than on the north, but temperature, based on corresponding altitude, southern slope is lower than the north. The snow line on the southern side generally is situated between 5500 m每6100 m above sea level, which is lower than northern slope (5800 m 每6200 m above sea level). Due to abundant rain and deep velley on the southern slope of Mt. Jolmo-Lungma region, there developed diversified forest vegetation; but on the north, there are fewer rainfall, the slight-cutting surface of the topography with many broad valleys and basins dominated by steppe. The vertical vegetational belt on the southern slope of Mt. Jolmo-Lungma region may be divided as follows: Below altitude 1000 m.: Monsoon rain forest of Shorea robusta. Between 1000 m每2500 m: Evergreen bra0d-leaf forest of Castanopsis indica and Schima wallichii etc. (the lower part of this belt) and Quercus glauca and Lithocarpus grandifolius, etc. (the upper part of this belt). The dividing line between these two part situated at an altitude of about 2000 m. Between 2500 m每3000 m (3100 m): The belt of mountain needle and broadleaf mixed forest consituted from Tsuga dumosa (= T. yunnanensis) and Quercus semicarpifolia, their pure forest and mixture. From 3000 m (3100 m) up to 3800 m. 4100 m.: Sub-alpine needleaf forest, in which Abies spectabilis is predominant. From this belt upward, there developes the alpine vegetation. Between 3800 m. (每4100 m) 每4500 m. (4600 m): Alpine bushwood of Rhododendron hamaetortum., Rh. setosym and Sabina reeurva, S. wallichiana. Between 4500m. (4600m) 每5200m: Alpine meadows of Kobresia pygmaea, K. kangtingensis, K. royleana and Carex atrata var. glacialis. From this belt upward, Lichen and bare rock belt are found upward to the snow line (5200每5500 m or 5600 m), among which, common species are: Rhizocarpon geographicum, Glypholecia scabra, Pertusaria sp., Umbilicaria tornata, Caloplaca elegans and Parmelia conspersa, etc. The vertical vegetation belt on the northen slope of Mt. Jolmo-Lungma distributes as follows: From 3900 m to 4400 m i. e. from south bank of Tsang-po (Brahmaputra) upward: Steppe formed by Pennisetum flaccidum, etc. Between 4400每5000 m (5100 m): Alpine steppe of Stipa purpurea. Between 5000 m, (5100 m) 每5700m: Alpine meadows of Carex atrata var. glacialis and Kobresia spp. Lichen-bare rock belt distributes between alpine meadow up to the snow line (5800 m每6200 m.), the species of lichen are similar to those of southern slope. Comparing the above with the vertical vegetation belt of temperate zone (Sit. Chang-bat), subtropical zone (mountainous region of Western Szechuan province) and tropical zone (Eastern and Western parts of Mt. Himalaya and mountain of Mexico), the authors hold that the southern slope of Mt. Jolmo-Lung Taa region belongs to the type of tropical (Northern marginal) mountainous vertical vegetation belt.
Abstract (Browse 2157)  |  Full Text PDF       
Changes in the Nucleic Acid and Protein Contents During the Growth of the Root Tips of Pea Seedlings
Author: Chang Wei-ping
Journal of Integrative Plant Biology 1973 15(2)
    The 5 mm long root tips of pea seedlings that had germinated 3 days at 25 ⊥were each cut into twenty 0.25 mm sections. The RNA and DNA contents of the sections were determined by the method of Ogur and Rosen, and their nitrogen con-tents of digested protein, by Conway's method of microdiffusion. The RNA and DNA contents determined were maximum at distances between0.5 mm and 1.5 mm from the tip of the root, whereas the relative content of nucleicacid in the tissue was maximum at the meristem of the root. The relative content of nucleic acid decreased continuously during the growth of the tissue. The elongation growth of the root ceased at the time when the relative content of nucleic acid dropped to a constant level. Possibly, a high content of nucleic acid was essential for maintaining the tissue's capacity for growth. Thus, when the nucleic acid content haddropped to its final constant level, tissue growth stopped. Moreover, under all experimental conditions, the changes in protein content at different distances from the tip of the root were similar to the changes in nucleic acid content.
Abstract (Browse 1715)  |  Full Text PDF       
The Formation of Rubber and Differentiation of Cellular Structures in the Secretory Epidermis of Fruits of Decaisnea fargesii Franch.
Author: Hu Zheng-hao and Tien Lan-hsing
Journal of Integrative Plant Biology 1973 15(2)
    Using the histochemical and cytological method of Bromine-Iodine-Glacial aceticacid fluid, we have observed the location, time, and processes of rubber formation inthe pericarp of Decaisnea fargesii and the ontogenetic changes of the epidermal cellsin these processes of the same plant. The following results are presented: 1. The formation of rubber is limited in the protoplasm of the secretory epidermis of pericarp. Generally, about ten days after blooming and fertilization, the rubbergranules begin to appear in the epidermal cells which are located in the depressionsof the young pericarps. And then, following the formation and development of thesecretory epidermis, the rubber granules increase progressively and stop till 60 daysold. when the secretory epidermis lyses into laticiferous canals, the formation of rubbergranules disappears. The rubber is stored up within the laticiferous canals. 2. During the course of fruiting, the epidermal cells of the carpel wall differentiate into the pericarpial epidermis and the secretory epidermis. To adapt it-self to its peculiar functions, each part forms its own particular feature, which isprominently different from the other. The cells of the secretory epidermis in cluding dense protoplasm, enlarge rapidly, and retain their vigorous activity. During thisperiod, they form rubber granules successively. Finaly when these cells are full of granules, they lose their vital functions andlyse into laticiferous canals. 3. The limitation of distribution and formation period of the rubber in Decaisnea fargesii is closely correlated with the ontogenetic processes of, its secretoryepidermis. This characteristics has never been observed, in other rubber plants. Decaisnea fargesii may provide a satisfactory material for the study of the mechanismof rubber biosynthesis. We hope that the present observation will provide some useful information for the improvement of rubber yield in this plant.
Abstract (Browse 1951)  |  Full Text PDF       
Cytological Studies on the Androgenesis of Triticale
Author: Sun Chin-san, Wang Chin-ch邦 and Chu Zhih-ching
Journal of Integrative Plant Biology 1973 15(2)
    This article deals with a study of the method in inducing pollen plants fromcultured anthers and cytological observations on the in vitro androgenesis of Triticale. The results obtained are summarized as follows: 1. Cell divisions of the uninucleate pollen grains and development of the am-phihaploid plants of Triticale can be readily obtained from anthers cultured on a basicmedium (slightly modified MS medium) supplemented with an appropriate amount of coconut milk and auxins (2,4-D, NAA). 2. The capacity of callus production varied greatly in different Triticalestrains. The pollens in the anthers of non-callus producing strains is potentially capable in producing embryoids, but the embryoids produced by them degenerate in anearly stage of development. 3. A higher proportion of the planted anthers producing shoots can be obtained, if the anthers containing embryoids had been transfered at a suitable time intothe medium for differentiation before the embryoidal cells developed into callus. 4. The two nuclei formed by the first division of the uninucleate microsporeare similar in appearance, they, however, generally differ in physiological functions. One of the nuclei having the nature of generative nucleus may divide and producefree nuclei which are eventually excluded in the course of embryoid development; the other having the nature of vegetative nucleus undergoes normal mitoses andtherefore plays an essential role in the embryoid formation. 5. In the differentiating or meristematic cells the starch accumulation is generallyrare, while in the cells surrounding them there are numerous starch grains.
Abstract (Browse 1841)  |  Full Text PDF       
A Study on the Pollen Morphology of Thalictrum L.
Author: Shi Yi-chen
Journal of Integrative Plant Biology 1973 15(2)
    The pollen morphology of 45 species of Chinese Thalictrum has been studied. According to the data from literature and our own material, we may draw the following conclusion: the pollen grains of Thalictrum are quite homogeneous in morphological characters. The pollen grains are spharoidal, pantoporate, with the dia-meter from 13 to 28米. The pores usually 6每18 in number, are more or less differentin size, number, and arrangement as well as the granular number on membrane. Exinein 1.7每3.1米 in thickness, 2-layered. The whole surface is covered with minute spines, loosely spaced and it is granulate in texture. The outline of the pollen grains isalmost circular in optical section. There is a close resemblance between the pollen grains of Thalictrum and thoseof Altingia, Liquidambar, and Chenopodiaceae. All of them are spharoidal and pantorate. But the pores of the pollen grains in Thalictrum L. are inconspicuous, definition usually indistinct, margin irregular, while the reverse is the case with pollengrains of Altingia and Liquidambar. The difference between the pollen grains of Thalictrum L. and those of Chenopodiaeeae is more marked. In the former the poresare greater in size and fewer in number, in the latter they are small and more nume-rous (30每90). The present study reveals the uniformity in the pollen type of Thalietrum. Thisgenus had been divided into several genera by some taxonomists but W. T. Wang and others reduced it into one genus Thalictrum. The palynological study supportsWang's conclusion. On the basis of the number and shape of chromosome, Thalictrumresembles Aquilegia and Isopyrum. In the external morphology of plants there isa close resemblance between Thalictrum and Enemion. While in the morphologicalcharacters of the pollen grains, Thalictrum is related to Asteropyrum.
Abstract (Browse 1933)  |  Full Text PDF       
Progress in Research of Plant Cell Culture and Somatic Cell Hybridization
Author: Laboratory of Cell Physiology, Institute of Plant Physiology, Shanghai
Journal of Integrative Plant Biology 1973 15(2)
Abstract (Browse 1743)  |  Full Text PDF       
Purification and Crystallization of Mo-Fe Protein, a Component of Nitrogenase from Azotobacter vinelandii
Author: 7th Laboratory, Institute of Botany, Academia Siniea
Journal of Integrative Plant Biology 1973 15(2)
Abstract (Browse 1595)  |  Full Text PDF       
Studies on the Morphological Differentiation of Excised Stems of Mentha Haplocalyx Briq.II. The Effect of MH upon the Excised Stem Tips
Author: Lee Cheng-lee and Kwei Yao-lin
Journal of Integrative Plant Biology 1973 15(2)
    The excised stem tips of Mentha haplocalyx Briq. L. had been cultured in vitro. Seven days after culturing, various aqueous concentrations of MH were added to make 0.1 ppm., 1 ppm., 10 ppm., and 100 ppm, in the culture solution, as soon as some of the adventitious roots had been established at the base. In low concentrations of MH (0.1 ppm, and 1 ppm.), the explanted stem tipsnormally developed and, instead, the growth was slightly promoted. But under the higher concentrations of MH (esp. in 100 ppm.), the activities of apical meristem, after two weeks of treatment, had been greatly reduced or completely inhibited and no further extention in length could be discerned. The distal ends of already grown adventitious roots showed elliptical swellings and simultaneously the roots ceased toelongate. Longitudinal sections of the tumefacient part exhibited remarkable increment in size of the cortical cells which were separated from the stele. Descriptions of the morphological and anatomical changes of the young leaves treated with the higher concentrations of MH were also presented.
Abstract (Browse 2049)  |  Full Text PDF       


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