February 1975, Volume 17 Issue 2


          Research Articles
Haploid Plantlet of Brassica campestris ¡ªFrom Anther Culture in Vitro
Author: Laboratory of Genetics, Kuangtung Institute of Botany
Journal of Integrative Plant Biology 1975 17(2)
Abstract (Browse 1948)  |  Full Text PDF       
Certain Factors Affecting the Frequency of Induction of Wheat (Triticum vulgare) Pollen Plants
Author: Pan Ching-li, Pas Shou-hsin, Kuan Chi-liang and Yu Hui-hsia
Journal of Integrative Plant Biology 1975 17(2)
    A study of certain factors affecting the induction and differentiation of wheat pollen callus cultured in vitro has been made. The results arc summarized as follows: 1. Sucrose in the medium plays an important role as the source of carbon and the regulator of osmotic pressure. The optimum concentration of sucrose is 10%. 2. Casein hydrolysate is obviously beneficial to the callus formation, the subsequent differentiation of organs and the diploidization of chromosomes. 3. When the spike of the wheat is pretreated at 3¨C5 ¡æ for 48 hours, the frequency of induction is greatly increased. 4. Higher temperature may promote the callus induction and the ability of organ differentiation. 5. Genotypic differences have a definite effect on the induction frequency of the pollen plantlets.
Abstract (Browse 1910)  |  Full Text PDF       
Studies of Some Biological Characteristics of Sporophore of Ganoderma lucidum (Leyss. ex Fr.) Karst. in Culture
Author: Lu Wen-liang and Chang Yung-ching
Journal of Integrative Plant Biology 1975 17(2)
    The present article deals with the morphological and the anatomical observations on the mature sporophores in Ganoderma lucidum. The developmental morphology of the exochite and the basidiospores have been investigated, inter alia, in some detail. After investigating the effects of CO2 gas on the sporophore culture the authors consider that when CO2 content in the atmosphere is increased to 0.1% it promotes the growth of the stipe and inhibits the growth and differentiation of the pileus, and this is the main cause of the formation of the deformed sporophores. At the same time it has been observed that the deformed sporophores which did not give rise to pileus could produce pileus if CO2 content in the atmosphere decreased. It is suggested that in the early stage of the sporophore culture, the CO2 content in the atmosphere should be increased adequately in order to promote the growth of the stipe, in the later stage CO2 content should be decreased by ventilation to induce the differentiation and growth of the pileus; thus disadvantageous factors may be converted into advantageous ones. The basidiospore germination has been obtained by the liquid culture with 10%¨C20% sporophore extract.The germination percentage is about 8%¨C15%. Thus, a new method for spawn rejuvenation is found.
Abstract (Browse 2047)  |  Full Text PDF       
Artificial Culture of Embryo in Citrus
Author: Wang Ta-yung and Chang Chin-jen
Journal of Integrative Plant Biology 1975 17(2)
    In an attempt to promote the efficiency in citrus breeding and estimate the possibility of embryo culture applied to citrus production, we carried out the culture technique in the hybrid embryos (Citrus sinensis Osbeek ¡Á Poncirus trifoliata Raf.). The results are summarized as follows: The fifth small embryos of the hybrid developed into seedings on White's medium containing IAA (l ppm) and CH (600 ppm) and on the MT medium containing GA (1 ppm), but some of these embryos cultured on the MT medium containing 2, 4-D (0.7 ppm) and BA (0.23 ppm) produced callus at first, and formed roots and plantlets from the original embryos later on. When the callus tissues excised from the embryos were transferred into the MT medium supplemented with BA (5 ppm) and IAA (1 ppm), they produced buds and developed into shoots, but no root was formed. When they were again transferred into a medium containing NAA (1 ppm) only, they could form roots and they developed into perfect plantlets as well. According to these studies it seems practical that the embryo culture technique could be used to study the distribution of zygote embryos and to overcome the interference of nucellus embryos in citrus breeding.
Abstract (Browse 2045)  |  Full Text PDF       
A Study on the Principle and Practice in the Culturing of Winter Wheat for High Yield
Author: Institute of Crop Science, Peking Academy of Agriculture
Journal of Integrative Plant Biology 1975 17(2)
    Deep plowing and adding of organic fertilizers to improve soil fertility are the foundations of high and stable yield for wheat. In a highly fertilized soil, seeds may be sown at a reasonably early date. In so doing a good growth of individual plants, a uniform population with a good canopy, and a quite high rate of effective tillers can be expected. In our experiments we have adopted the following cultural routine: (a) A good choice of high yielding variety (our choice is A. U. 139). (b) A reasonable early date of sowing. (c) A sowing of 100,000 seedlings per mu (666.6 m2). (d) A total of 700,000 tillers per mu before over-wintering (about 1.2 times the number of heads per mu) and more than 1,200,000 tillers per mu after wintering (about twice the number of heads per mu).(e) Finally, a total of 550,000¨C600,000 heads per mu when ripe. (There will be more than 26 grains per head, a 1,000 grains will weigh 36 g or higher, and the yield will be more than 500 kg per mu). In the district of Peking where winter wheat is usually sown in the autumn, the most important factor for high productivity is the rational control of spring tillering so as to achieve a good canopy. When wheat land is highly fertilized and its moisture condition is good, the final watering (during the stage of grain filling) is applied about 20 days prior to maturity. A reasonable control of soil fertility (mainly available soil N) by proper management of irrigation is a profitable means to regulate the N-C metabolism to attain a higher weight of 1,000 grains.
Abstract (Browse 1975)  |  Full Text PDF       
Studies on ¡°Growth Center¡± and ¡°Growth Center Leaf¡± at Different Stages of Development in the Corn Plant
Author: Jen Chi-yun
Journal of Integrative Plant Biology 1975 17(2)
    The present article gives an account of the relationship between "growth center" and ¡°growth center leaf" (leaf responsible for supply of material for growth) during defferent stages of growth in the corn plant. Knowledge gained from such studies is expected to facilitate devising rational procedures for crop cultivation and management.
Abstract (Browse 1983)  |  Full Text PDF       
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