January 1979, Volume 21 Issue 1

 

          Research Articles
The Variation of the Contents of Two Alkaloids in Anisodus tanguticus
Author: Wang Zhi-bin and Wu Xian-qi
Journal of Integrative Plant Biology 1979 21(1)
Abstract (Browse 1717)  |  Full Text PDF       
The Observation of Microchemical Colour Reaction of Andrographis Pantculata (Burm. F.) Nees Tissue and its Crystals of Lactone
Author: Jiang Rong-lan
Journal of Integrative Plant Biology 1979 21(1)
Abstract (Browse 1770)  |  Full Text PDF       
The Isolation of the Ecdysterone from Taxus Chinensis var. Mairei (Lemee et Levl) Cheng et L. K.Fu
Author: Nie Rui-lin
Journal of Integrative Plant Biology 1979 21(1)
Abstract (Browse 1891)  |  Full Text PDF       
Examination on seed Oils Six Species in Xizhang
Author: Zhang Qing-hua, Meng Shao-jiang, Wang Jing-ping and He Guan-fo
Journal of Integrative Plant Biology 1979 21(1)
Abstract (Browse 1626)  |  Full Text PDF       
The Status and Prospects of Organic Synthetic Works in Phytochemistry
Author: Gao Yi-sheng
Journal of Integrative Plant Biology 1979 21(1)
Abstract (Browse 1851)  |  Full Text PDF       
Influence of Environmental Factors and Age of Plant on the Control of Bermudagrass by Glyphosate
Author: Weed Control Section, Laboratory of Phytohormone, Shanghai Institute of Plant Physiology, Academia Sinica
Journal of Integrative Plant Biology 1979 21(1)
      
    The influence of environmental factors and age of plant on the control of bermudagrass was studied in the phytotron. The results showed that glyphosate gave better control on bermudagrass under high light intensity and high soil hmnidity than under low light intensity and low soil humidity. During the first two weeks the efficiency of glyphosate treatment differed markedly; however, the difference between these different conditions deminished with the increase in concentration or with prolonged treatment. The environmental factors and concentration of glyphosate also exerted a significant influence on the resumption of the plant growth thereafter. The lower the light intensity the less was the potential capacity for resumption of the growth. In our experiments there was no resumption of growth after the treatment with glyphosate at a concentration of 0.4%. The response of bermudagrass at different ages to glyphosate was significantly different. The older the plant the higher was the percentage of the survival and the resumption of the plant growth.
Abstract (Browse 1699)  |  Full Text PDF       
Chemical Components in the Bark of Magnolia rostrata W. W. Smith
Author: Yen Wen-mei
Journal of Integrative Plant Biology 1979 21(1)
      
    From the bark of Magnolia rostrata W. W. Smith (Magnoliaceae), the honokiol(1) mp. 87.5 ⊥ and the magnolol (j) mp. 102 ⊥ was isolated. Moreover, it can be proved that the bark of Magnolia rostrata W. W. Smith contains 汕-eudesmol in the essential oil.
Abstract (Browse 1998)  |  Full Text PDF       
Changes of Photochemical Activities and Photosynthetic Electron Transport of Chloroplasts in the Course of Ageing
Author: Lu Rong-zhao, Yu Yan-li and Wang Shu-zhi
Journal of Integrative Plant Biology 1979 21(1)
      
    Chloroplasts were isolated from Spinacia olerecea L. and Doblichos lablab L. Chloroplasts suspension was stored in refrigerator at 5每8 ⊥. Photochemical activities and chlorophyll content of chloroplasts at different times of storage were deter- mined. The results can be summarized as follows: 1. In the course of chloroplasts ageing, the lost of K3Fe(CN)6 photo reduction activity was more than that of DCPIP photoreduction activity. 2. The activity of K3Fe(CN)6 photoreduction during storage began to decrease markedly after 12 hours, but activity of DCPIP photoreduction began to decrease markedly after 24 hours. 3. The DCPIP photoreduction activity of aged chloroplasts was stimulated by the addition of 1.5-diphenylcarbazide. 4. Destruction of oxidized side of PSj was earlier and higher than that of the other side (from the active center of PSj to the reduced side of PSi). 5. During chloroplasts ageing, the decrease of chlorophyll content was less than the rate of decrease of photochemical activities.
Abstract (Browse 1952)  |  Full Text PDF       
Studies on the Chemical Constituents of Cyclea hypoglauca (Shay.) Diels (Menispermaceae)
Author: Zhou Fa-xing and Xu Xue-jian
Journal of Integrative Plant Biology 1979 21(1)
      
    Five crystalline substances were isotated from the roots and the rhizomas of Cyclea hyboglauca (Shau.) Diels and were identified as d-quercitol, 汕-sitosterol, cycleanine, 1-curine and cyclanoline respectively.
Abstract (Browse 1799)  |  Full Text PDF       
Pollen Morphology of Guttiferae in China
Author: Xi Yi-zhen
Journal of Integrative Plant Biology 1979 21(1)
      
    The present paper deals with the pollen morphology of 23 species belonging to 6 genera of Guttiferae occurred in China. All pollen grains have been observed under light microscope, and those of 11 species in 5 genera have been examined with SEM as well. The pollen grains of Guttiferae are subspheroidal, spheroidal or prolate, (17每43) ℅ (15每26)米 in size. Mostly 3-eolporate, less frequently 4-colporate or 5-colporate. The exine is 2-layered, but the occassionally demarcation of these layers is generally indistinct. The exine is 0.8每2.6 米 in thickness. The oramentations of all the pollen grains is generlly finely reticulate under the light microscope. Pollen morphology of the 11 species of 5 genera observed under the SEM is stressed. Their exine oramentations may be classified into four major groups, ie. reticulate, finely reticulate, baculate and perforate. Calophyllum: pollen grains reticulate and rugulo-reticulate. Cratoxylon: pollen grains reticulate, with finely and densely granulate over entire muri. Gareinia: pollen grains baculate, reticulate, and exine with perforate oramentation; in reticulate grains, muri with spinulation. Hypericum: pollen grains reticulate, perforate and finely reticulate. Mesua: pollen grains finely reticulate. From the comparison of pollen morphology, Hypericaceae has much connection with Guttiferae (sensu stricto). Because their pollen grains are all 3-eolporate and the exine is reticulate or oerforate under light mieroseope, but it is different between the pollen grains of Hypericum and those of Guttiferae.
Abstract (Browse 2090)  |  Full Text PDF       
The Isolation of DNA from Plant Materials
Author: Lin Zhong-ping, Liu Hong-jun, Wu Shi-jun, Ma Cheng, Zhao Yu-jin and Wang Yu-xiu
Journal of Integrative Plant Biology 1979 21(1)
      
    Total DNA was isolated from seedling and tissues of many plants. The nuclei and chloroplasts were prepared from plant tissues, and then the nuclear DNA and chloroplast DNA were isolated from them. According to the chemical analysis and the physical properties determined by ultraviolet absorbance, hyperchromicities, ultracentrifugation, gel electrophoresis and the electro-microscopical observations it is suggested that DNA obtained possessed a considerable purity and to a certain extent retained the natural status of the large molecule.
Abstract (Browse 1811)  |  Full Text PDF       
New Species of the Late Triassic Plants from Yanbian, Sichuan, I
Author: Chen Ye, Duan Shu-ying and Zhang Yu-cheng
Journal of Integrative Plant Biology 1979 21(1)
      
    This paper deals with eight plants, all new to science of late Triassic (Keuper), found in the district Yanbian, Sichuan. These plants are Angiopteris hongniensis, Marattia antiqua, Todites yanbianensis, T. subtilis, Hicriopteris triassica, Kylikipteris simplex, Ctenozamites microloba, C. otoeis.
Abstract (Browse 1807)  |  Full Text PDF       
The Gametophytes and Fertilization in Pseudotaxus
Author: Chen Zu-keng and Wang Fu-xiong
Journal of Integrative Plant Biology 1979 21(1)
      
    The development of the gametophytes and fertilization of Pseudotaxus chienii Cheng has been investigated. Pollination first occurred on April 17 (1964). The pollen grains shed at the uninucleate stage and germination on the nucellus is almost immediate. The pollen tubes approached the freenucleate female gametophyte about May 5. The spermatogenous cell is continuously enlarging with the growth of the pollen tube and two unequal sperms are formed after its division. Occasionally the small sperm may divide further into two smaller ones. During pollination the megaspore mother cell is in meiosis and 3 or 4 megaspores are formed. Generally 2 or 3 megaspores at the micropylar end are going to degenerate while the chalaza] megaspore is rapidly enlarging. After 8 successive simultaneous divisions of the functional megaspore 256 free nuclei are resulted and they are evenly distributed at the bulge of the famale gametophyte. Then the wall formation follows. Sometimes there are more than two, even as many as 5每6 gametophytes developed within a single ovule. The archegonial initials become differentiated at the apical end of the female gametophyte. They are usually single and apical, rarely lateral in position. The number of the archegonia vary from 3 to 7, usually 4每6. There are 2每8 neck cells in each archegonium which is surrounded by a layer of jacket cells. The central cell divided about May 20每26 (1964) and the division of the central cell gives rise to the egg and the ventral canal nucleus, the latter being degenerated soon. There are many proteid vacuoles near the nucleus of the matured egg. The fertilization took place about May 23每26 (1964). At first, the pollen tube discharges its contents into the egg, then the larger sperm fuses with the egg nucleus in the middle part of the archegonium. At the same time the male cytoplasm also fuses with the female cytoplasm and a layer of densely-staining neocytoplasm is formed around the fused nucleus. The smaller sperm, tube nucleus and sterile cell usually remain in the cytoplasm above the egg nucleus for some time. Based upon the observations of the development of the gametophytes and fertilization the authors conclude that Pseudotaxus is more close related to Taxus than any other member of Taxaceae.
Abstract (Browse 1810)  |  Full Text PDF       
The Fusion of Male and Female Nuclei in Fertilization of Higher Plants
Author: Hu Shi-yi and Zhu Cheng
Journal of Integrative Plant Biology 1979 21(1)
      
    Studies on the fusion of male and female nuclei in fertilization of Helianthus an- nuus L., Triticum aestivan L., Gossypium hisutum L., Hosta caerulea Tratt., and Pinus tabulaeformis Carr. were made in the present work. The results are summarized as follows: 1. The essential process of the fusion of male and female nuclei during syngamy in four species of angiosperms studied may' be generalized as follows: (1) the male nucleus made contact with the female one, (2) followed by the fusion of nuclear membranes between the male and female nuclei. (3) then the despiralization of male spireme happened and male nucleolus made its appearance inside of the fertilized egg nucleus (4) the male chromatin dispersed and make its appearance indistinguishable from that of the female chromatin, (5) the male and female nucleoli fused together to form a larger nucleolus as a sign of completion of the fusion of the two nuclei. In the first mitotic division of the zygote there was only one common mitotic spindle. 2. The essential process of the fusion of egg and sperm nuclei during syngamy in a gymnosperm-Pinus tabulaeformis could also be outlined as follows: (1) the sperm nucleus made contact with the egg nucleue, (2) the fusion of nuclear membranes happened between the male and female nuclei, (3) the male and female ehromatins condensed to form two separate groups of chromatin threads together with the very apparent apperance of the male and female nucleoli at this stage, (4) the male and female chromosomes grouped respectively in their own spindles while both nucleoli disappeared, (5) then the two spindles fused together and all the chromosomes arranged to form a common equatorial plate, (6) finally two daugter nuclei resulted from the mitotic division. 3. Based on the facts that there were two different patterns of the fusion of male and female nuclei in fertilization discribed, all of these accounts are in general accord with the condition usually described that there are two types of fertilization, the pre- mitotic and postmitotie syngamy in higher plants. The type of angiosperm fertilization and the mechanism of promoting the zygote to divide after fertilization are discussed, and the nuclear fusion in sexual reproduction has been compared with that of somatic cell hybridization.
Abstract (Browse 4076)  |  Full Text PDF       
Plantlets from Isolated Pollen Cultures of Eggplant (Solanum melongena L.)
Author: Gu Shu-rong
Journal of Integrative Plant Biology 1979 21(1)
      
    The present paper reports the experimental results on isolated pollen cultures of eggplant. The pretreatment of culturiug pollen grains within the anthers for four days before isolation was beneficial to the division of pollen grains and formation of calluses. The best liquid medium tested for isolated pollen cultures was composed of MS maeroelements and supplemented with 800 mg/l glutamine, 100 mg/l serine, 5 g/l myoinositol, 2 mg/l 1, 4-D and 1 mg/l kinetin. The formation of pollen calluses was mostly derived from the division of the vegetative nucleus, and a part of calluses was developed from the equal division of the microspores. After the isolated pollen grown in suspensions for about 20 days under static culture the fresh medium was added and at the same time the cultures were kept under rotating condition. These measures were beneficial to the production and development of calluses. When the calluses grew up to 3每4 mm. in diameter, they were transferred to the semisolid medium in which MS medium was used as the basic one for differentiation of the organs. Plantlets were finally formed from the pollen calluses derived from the cultivar, "the nine-leaved eggplant.
Abstract (Browse 1997)  |  Full Text PDF       
The Research of Giemsa C-Banding Technigue Used in Plant Chromosome Banding Pattern
Author: Chen Rui-yang, Song Wen-qin, Chen Xiao, Li De-cheng, Xu Yue-fan, Fan Hai-ping and Zhou Xian-chang
Journal of Integrative Plant Biology 1979 21(1)
      
    The Giemsa banding technique used by Fiskesjo in Allium cepa (1974) was modified, and used successfully in band showing of Vicia faba, Secale cereale, Zea mays. Hordeum vulgare, Triticum aestivum, and Triticale. The C-banding characteristics of these crops have been analysed.The band showing effects of ItSG method have been compared with those of BSG method.The banding conditions in plant chromosomes have been discussed.
Abstract (Browse 2066)  |  Full Text PDF       
 

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