| Special Issue: Genomics-assisted Germplasm Improvement in Rice|
|| Ectopic expression of fungal EcGDH improves nitrogen assimilation and grain yield in rice
Author: Dongying Tang, Yuchong Peng, Jianzhong Lin, Changqing Du, Yuanzhu Yang, Dan Wang, Cong Liu, Lu Yan, Xiaoying Zhao, Xia Li, Liangbi Chen and Xuanming Liu
Received: December 28, 2016 Accepted: January 5, 2017
Online Date: January 6, 2017
| |Abstract (Browse 114) | References | Full Text HTML | Full Text PDF
NADP(H)-dependent glutamate dehydrogenases (GDH) in lower organisms have stronger ammonium affinity than those in higher plants. Here we report that transgenic rice overexpressing the EcGDH from Eurotium cheralieri exhibited significantly enhanced aminating activities. Hydroponic and field tests showed that nitrogen assimilation efficiency and grain yields were markedly increased in these transgenic plants, especially at the low nitrogen conditions. These results suggest that EcGDH may have potential to be used to improve nitrogen assimilation and grain yield in rice.
|| QTL editing confers opposing yield performance in different rice varieties
Author: Lan Shen, Chun Wang, Yaping Fu, Junjie Wang, Qing Liu, Xiaoming Zhang, Changjie Yan, Qian Qian and Kejian Wang
Received: June 1, 2016 Accepted: September 13, 2016
Online Date: September 15, 2016
| |Abstract (Browse 203) | References | Full Text HTML | Full Text PDF
Grain yield is one of the most important and complex trait for genetic improvement in crops; it is known to be controlled by a number of genes known as quantitative trait loci (QTLs). In the past decade, many yield-contributing QTLs have been identified in crops. However, it remains unclear whether those QTLs confer the same yield performance in different genetic backgrounds. Here, we performed CRISPR/Cas9-mediated QTL editing in five widely-cultivated rice varieties and revealed that the same QTL can have diverse, even opposing, effects on grain yield in different genetic backgrounds.