%A Aixia Li, Wenlong Yang, Xueyuan Lou, Dongcheng Liu, Jiazhu Sun, Xiaoli Guo, Jing Wang, Yiwen Li, Kehui Zhan, Hong-Qing Ling, and Aimin Zhang %T Novel Natural Allelic Variations at the Rht-1 Loci in Wheat %0 Journal Article %D 2013 %J J Integr Plant Biol %R 10.1111/jipb.12103 %P 1026-1037 %V 55 %N 11 %U {https://www.jipb.net/CN/abstract/article_21528.shtml} %8 2013-11-05 %X

Plant height is an important agronomic trait. Dramatic increase in wheat yield during the “green revolution” is mainly due to the widespread utilization of the Reduced height (Rht)-1 gene. We analyzed the natural allelic variations of three homoeologous loci Rht-A1, Rht-B1, and Rht-D1 in Chinese wheat (Triticum aestivum L.) micro-core collections and the Rht-B1/D1 genotypes in over 1,500 bred cultivars and germplasms using a modified EcoTILLING. We identified six new Rht-A1 allelic variations (Rht-A1b–g), eight new Rht-B1 allelic variations (Rht-B1h–o), and six new Rht-D1 allelic variations (Rht-D1e–j). These allelic variations contain single nucleotide polymorphisms (SNPs) or small insertions and deletions in the coding or uncoding regions, involving two frame-shift mutations and 15 missenses. Of which, Rht-D1e and Rht-D1h resulted in the loss of interactions of GID1-DELLA-GID2, Rht-B1i could increase plant height. We found that the Rht-B1h contains the same SNPs and 197 bp fragment insertion as reported in Rht-B1c. Further detection of Rht-B1h in Tibet wheat germplasms and wheat relatives indicated that Rht-B1c may originate from Rht-B1h. These results suggest rich genetic diversity at the Rht-1 loci and provide new resources for wheat breeding.

Li A, Yang W, Lou X, Liu D, Sun J, Guo X, Wang J, Li Y, Zhan K, Ling HQ, Zhang A (2013) Novel natural allelic variations at the Rht‐1 loci in wheat. J. Integr. Plant Biol. 55(11), 1026–1037.