%A Donggiun Kim, So Yun Park, Youngjae Chung, Jongbum Park, Sukchan Lee, and Taek-Kyun Lee %T Biochemical Characterization of Soluble Acid and Alkaline Invertases from Shoots of Etiolated Pea Seedlings %0 Journal Article %D 2010 %J J Integr Plant Biol %R 10.1111/j.1744-7909.2010.00937.x %P 536-548 %V 52 %N 6 %U {https://www.jipb.net/CN/abstract/article_21948.shtml} %8 2010-03-16 %X
Soluble invertase was purified from pea (Pisum sativum L.) by sequential procedures entailing ammonium sulfate precipitation, DEAE-Sepharose column, Con-A- and Green 19-Sepharose affinity columns, hydroxyapatite column, ultra-filtration, and Sephacryl 300 gel filtration. The purified soluble acid (SAC) and alkaline (SALK) invertases had a pH optimum of 5.3 and 7.3, respectively. The temperature optimum of two invertases was 37 °C. The effects of various concentrations of Tris-HCl, HgCl
Kim D, Park SY, Chung Y, Park J, Lee S, Lee TK (2010) Biochemical characterization of soluble acid and alkaline invertases from shoots of etiolated pea seedlings. J. Integr. Plant Biol. 52(6), 536–548.