%A Wang Guo-qiang, Peng Jian-xin, Qian Yue-qin, Li Rong and Zhang Zheng-fu %T Isolation and Characterization of Phosphatase from Thylakoid Membranes of Spinach Chloroplasts %0 Journal Article %D 1995 %J J Integr Plant Biol %R %P -${article.jieShuYe} %V 37 %N 4 %U {https://www.jipb.net/CN/abstract/article_23720.shtml} %8 %X A phosphatase from thylakoid membrane of spinach (Spinacia oleracea L. ) chloroplasts was isolated with the methods of extraction with n-ButanoL centrifugation at 100000 g for 30 min and chromatographic separation through DEAE-Cellulose (DE 52) column.The phosphatase catalyzed hydrolysis of phosphate monoesters (4-nitrophenyl phosphate). The optimal pH for enzyme catalysis was below 7. The peak rate of the enzyme reaction was obtained when it was incubated at 60℃ for 15 min. The phosphatase was inhibited by ATP and phosphate. The results from SDS-PAGE showed that the preparation of enzyme was composed of two proteins.