%A Gu Shu-rong, Zhu Zhi-qing, Zhao Jing-fang and Chen Zheng-hua %T Induction and Differentiation of Callus from Female Gametohyte Explants of Pinus bungeana Zucc. and P. tabulaeformis Carr. %0 Journal Article %D 1995 %J J Integr Plant Biol %R %P -${article.jieShuYe} %V 37 %N 3 %U {https://www.jipb.net/CN/abstract/article_23734.shtml} %8 %X Immature endosperms (female gametophyte) of Pinus bungeana Zucc. and P. tabulaeformis Carr. were used as explants for establishing tissue cultures. Calli induction and differentiation were studied on a modified MS medium containing 3 % sucrose and various concentrations of auxins and cytokinins. Callus tissues of P. bungeana and P. tabulaeforms could be induced on media supplemented with 1--6 mg/L naphthoxyacetic acid and 0.5 mg/L 6-BAP. The highest induction frequency of calli was 250%. Histocytological observation revealed that the callus cell was haploidy with, N= 12. Differentiation of green buds occurred on the medium supplemented with 0. 1 mg/L ABA, whereas no plantlet was developed, however.