%A LUO An-Ding, CHEN Shou-Cai, WU Kun-Xin, FU Shao-Ping %T AFLP Fingerprinting Analysis of Elite Hevea brasiliensis Germplasm %0 Journal Article %D 2001 %J J Integr Plant Biol %R %P 941-947 %V 43 %N 9 %U {https://www.jipb.net/CN/abstract/article_25744.shtml} %8 %X
There are more than 6 000 clones of Hevea brasiliensis Mull. Ary germplasm in the germplasm garden of Chinese National Key Biotechnology Laboratory for Tropical Crops and some of them are elite germplasm demonstrated by production and previous studies. AFLP (amplified fragment length polymorphism) fingerprinting analysis was performed on 25 clones (15 of Wickham clones and 10 of Amazon wild clones which possess phenotypes with high-yielding/low-yielding, cold-tolerance/cold-sensitivity, oidium-resistance/oidium-sensitivity, tapping panel dryness (TPD)/healthy) respectively through a 377 DNA sequencer (P. E. Corp.) and PAGE electrophoresis results were analyzed by using GeneScanTMand GenotypeTM Analysis software (P.E.Corp.). The fragment profiles of different clones were obtained. Five hundred and eighteen fragments were generated by two primer combinations screened from 64 primer combinations and 511 fragments appeared to be polymorphic (98.6%). Genetic distance ranged from 0.25 to 0.81 between clones and ranged from 0.07 to 0.17 within RRIM600 clone. A specific 320 bp fragment of the oidium-resistant clones was found through genotype analysis. These results showed that AFLP fingerprints were highly reproducible and powerful and can be widely used in germplasm identification and genetic diversity analysis of Hevea brasiliensis. In addition, based on the AFLP data, cluster analysis was performed. Cluster results showed that all the clones studied were almost clustered into a group one by one.
AFLP 在橡胶树优异种质研究中的应用
罗安定 陈守才* 吴坤鑫 符少萍
(中国热带农业科学院热带作物生物技术国家重点实验室,海口571101)
关键词: 巴西橡胶;AFLP;种质鉴定;遗传多样性