%A Lu Tie-gang, Sun ,Jing-san and Zheng Guo-chang %T Hormonal Regulation and Effect of L-Proline on Somatic Embryogenesis of Sainfoin %0 Journal Article %D 1990 %J J Integr Plant Biol %R %P -${article.jieShuYe} %V 32 %N 10 %U {https://www.jipb.net/CN/abstract/article_26671.shtml} %8 %X The effects of BA and KT on somatic embryogenesis of sainfoin (Onobrychis viciaefolia Scop) have been studied. Light-yellow callus was able to be formed from sainfoin hypocotyl explants cultured on LS medium containing I mg/l BA+ 1 mg/1 KT, and its somatic embryogenesis was induced by 1 mg/l BA and inhibited by 1 rog/1 KT on LS medium. Analysis of free amino acid in different types of calli indicated that the content of L-proline in embryonic callus was only as much as 2/5 of that in nonembryonic callus. Addition of L-proline to culture medium led to the stimulative effects on somatic embryogenesis in sainfoin and its optimal concentration is 1000 mg/1.